Restriction map for
pF1KSDA0214
(Circular) (Six-base) MAPSORT of: pF1KSDA0214.seq Check: 6327 from: 1 to: 5408
pF1KSDA0214 5408 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1401 1401
Size: 5408
AatII G_ACGT'C
Cuts at: 359 2240 359
Size: 1881 3527
AccI GT'mk_AC
Cuts at: 1417 2446 1417
Size: 1029 4379
Acc65I G'GTAC_C
Cuts at: 2424 2424
Size: 5408
AclI AA'CG_TT
Cuts at: 5248 5248
Size: 5408
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1109 2290 3233 3665 4412 4680 718
Size: 391 1181 943 432 747 268 1446
Fragments arranged by size:
1446 1181 943 747 432 391 268
AflII C'TTAA_G
Cuts at: 211 211
Size: 5408
AflIII A'CryG_T
Cuts at: 302 1556 2363 2760 3864 4841 302
Size: 1254 807 397 1104 977 869
Fragments arranged by size:
1254 1104 977 869 807 397
AgeI A'CCGG_T
Cuts at: 3796 3796
Size: 5408
AleI CACnn'nnGTG
Cuts at: 1683 1683
Size: 5408
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 662 2282 662
Size: 1620 3788
AlwNI CAG_nnn'CTG
Cuts at: 351 2199 2382 2713 4280 351
Size: 1848 183 331 1567 1479
Fragments arranged by size:
1848 1567 1479 331 183
ApaI G_GGCC'C
Cuts at: 1801 1801
Size: 5408
ApaLI G'TGCA_C
Cuts at: 4178 4178
Size: 5408
ApoI r'AATT_y
Cuts at: 1143 1330 2412 4761 5017 1143
Size: 187 1082 2349 256 1534
Fragments arranged by size:
2349 1534 1082 256 187
AseI AT'TA_AT
Cuts at: 20 20
Size: 5408
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5408
AvaI C'yCGr_G
Cuts at: 925 1274 2243 2428 925
Size: 349 969 185 3905
Fragments arranged by size:
3905 969 349 185
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2362 2395 2362
Size: 33 5375
BamHI G'GATC_C
Cuts at: 2433 2433
Size: 5408
BanI G'GyrC_C
Cuts at: 1442 2424 2838 3086 3121 1442
Size: 982 414 248 35 3729
Fragments arranged by size:
3729 982 414 248 35
BanII G_rGCy'C
Cuts at: 577 926 1355 1801 1903 2045 2360 2422 3452
Size: 349 429 446 102 142 315 62 1030
Cuts at: 3452 577
Size: 2533
Fragments arranged by size:
2533 1030 446 429 349 315 142 102
62
BbeI G_GCGC'C
Cuts at: 1446 2842 3090 1446
Size: 1396 248 3764
Fragments arranged by size:
3764 1396 248
BbsI GAAGACnn'nnnn_
Cuts at: 305 1195 1748 305
Size: 890 553 3965
Fragments arranged by size:
3965 890 553
BbvCI CC'TCA_GC
Cuts at: 191 191
Size: 5408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 138 172 138
Size: 34 5374
BciVI GTATCCnnnnn_n'
Cuts at: 3306 4067 5408 3306
Size: 761 1341 3306
Fragments arranged by size:
3306 1341 761
BclI T'GATC_A
Cuts at: 461 2928 461
Size: 2467 2941
BfrBI ATG'CAT
Cuts at: 202 910 202
Size: 708 4700
BglII A'GATC_T
Cuts at: 2923 4960 2923
Size: 2037 3371
BlpI GC'TnA_GC
Cuts at: 2520 2520
Size: 5408
Bme1580I G_kGCm'C
Cuts at: 541 691 1801 3033 3126 4182 541
Size: 150 1110 1232 93 1056 1767
Fragments arranged by size:
1767 1232 1110 1056 150 93
BmrI ACTGGGnnnn_n'
Cuts at: 1170 1555 2620 2701 3036 5180 1170
Size: 385 1065 81 335 2144 1398
Fragments arranged by size:
2144 1398 1065 385 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 885 1188 3775 885
Size: 303 2587 2518
Fragments arranged by size:
2587 2518 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1369 1475 2275 1369
Size: 106 800 4502
Fragments arranged by size:
4502 800 106
Bpu10I CC'TnA_GC
Cuts at: 191 1043 2326 191
Size: 852 1283 3273
Fragments arranged by size:
3273 1283 852
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1346 1486 2584 3463 3955 4253 1346
Size: 140 1098 879 492 298 2501
Fragments arranged by size:
2501 1098 879 492 298 140
BsaI GGTCTCn'nnnn_
Cuts at: 5112 5112
Size: 5408
BsaAI yAC'GTr
Cuts at: 305 2400 2761 305
Size: 2095 361 2952
Fragments arranged by size:
2952 2095 361
BsaBI GATnn'nnATC
Cuts at: 6 2922 4959 6
Size: 2916 2037 455
Fragments arranged by size:
2916 2037 455
BsaHI Gr'CG_yC
Cuts at: 356 1443 1864 2237 2839 3087 3789 5285 356
Size: 1087 421 373 602 248 702 1496 479
Fragments arranged by size:
1496 1087 702 602 479 421 373 248
BsaWI w'CCGG_w
Cuts at: 2597 3118 3796 4070 4217 4628 2597
Size: 521 678 274 147 411 3377
Fragments arranged by size:
3377 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1555 1585 1982 2012 2152 2182 1555
Size: 30 397 30 140 30 4781
Fragments arranged by size:
4781 397 140 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 603 952 1167 1177 1447 1930 2006 2044 603
Size: 349 215 10 270 483 76 38 3967
Fragments arranged by size:
3967 483 349 270 215 76 38 10
BseYI C'CCAG_C
Cuts at: 261 902 990 1876 2389 2833 3591 4168 4664
Size: 641 88 886 513 444 758 577 496
Cuts at: 4664 261
Size: 1005
Fragments arranged by size:
1005 886 758 641 577 513 496 444
88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 1507 1246
Size: 261 5147
BsiEI CG_ry'CG
Cuts at: 104 2950 2996 4204 104
Size: 2846 46 1208 1308
Fragments arranged by size:
2846 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 457 1055 1355 2360 2422 3200 3390 4182 457
Size: 598 300 1005 62 778 190 792 1683
Fragments arranged by size:
1683 1005 792 778 598 300 190 62
BsmI GAATG_Cn'
Cuts at: 204 318 1976 204
Size: 114 1658 3636
Fragments arranged by size:
3636 1658 114
BsmBI CGTCTCn'nnnn_
Cuts at: 871 871
Size: 5408
Bsp1286I G_dGCh'C
Cuts at: 457 541 577 691 926 1055 1355 1801 1903
Size: 84 36 114 235 129 300 446 102
Cuts at: 1903 2045 2360 2422 3033 3126 3200 3390 3452
Size: 142 315 62 611 93 74 190 62
Cuts at: 3452 4182 457
Size: 730 1683
Fragments arranged by size:
1683 730 611 446 315 300 235 190
142 129 114 102 93 84 74 62
62 36
BspEI T'CCGG_A
Cuts at: 4628 4628
Size: 5408
BspHI T'CATG_A
Cuts at: 4584 4584
Size: 5408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1275 1401 2390 2458 2672 2974 3355 1275
Size: 126 989 68 214 302 381 3328
Fragments arranged by size:
3328 989 381 302 214 126 68
BsrBI CCG'CTC
Cuts at: 50 1314 3700 3754 5238 5403 50
Size: 1264 2386 54 1484 165 55
Fragments arranged by size:
2386 1484 1264 165 55 54
BsrDI GCAATG_nn'
Cuts at: 450 1430 2024 3320 450
Size: 980 594 1296 2538
Fragments arranged by size:
2538 1296 980 594
BsrFI r'CCGG_y
Cuts at: 649 1939 2338 3406 3587 3796 649
Size: 1290 399 1068 181 209 2261
Fragments arranged by size:
2261 1290 1068 399 209 181
BssSI C'ACGA_G
Cuts at: 3679 4037 3679
Size: 358 5050
BstAPI GCAn_nnn'nTGC
Cuts at: 1602 2785 1602
Size: 1183 4225
BstBI TT'CG_AA
Cuts at: 110 3769 110
Size: 3659 1749
BstEII G'GTnAC_C
Cuts at: 364 1935 364
Size: 1571 3837
BstXI CCAn_nnnn'nTGG
Cuts at: 750 978 750
Size: 228 5180
BstYI r'GATC_y
Cuts at: 1 2433 2900 2923 3258 3504 4516 4602 4614
Size: 2432 467 23 335 246 1012 86 12
Cuts at: 4614 4960 1
Size: 346 449
Fragments arranged by size:
2432 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1574 1931 1970 1574
Size: 357 39 5012
Fragments arranged by size:
5012 357 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3358 3539 3702 3358
Size: 181 163 5064
Fragments arranged by size:
5064 181 163
BtsI GCAGTG_nn'
Cuts at: 224 1190 1888 2015 2651 224
Size: 966 698 127 636 2981
Fragments arranged by size:
2981 966 698 636 127
ClaI AT'CG_AT
Cuts at: 1005 1005
Size: 5408
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 276 311 276
Size: 35 5373
DraI TTT'AAA
Cuts at: 2407 2407
Size: 5408
DraIII CAC_nnn'GTG
Cuts at: 855 855
Size: 5408
DrdI GACnn_nn'nnGTC
Cuts at: 1672 3114 3972 1672
Size: 1442 858 3108
Fragments arranged by size:
3108 1442 858
EaeI y'GGCC_r
Cuts at: 432 504 546 803 874 1427 1577 2993 3167
Size: 72 42 257 71 553 150 1416 174
Cuts at: 3167 3558 3585 4930 432
Size: 391 27 1345 910
Fragments arranged by size:
1416 1345 910 553 391 257 174 150
72 71 42 27
EagI C'GGCC_G
Cuts at: 2993 2993
Size: 5408
EarI CTCTTCn'nnn_
Cuts at: 142 445 1304 3431 3641 142
Size: 303 859 2127 210 1909
Fragments arranged by size:
2127 1909 859 303 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3616 3926 4072 5218 3616
Size: 310 146 1146 3806
Fragments arranged by size:
3806 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1353 2358 2420 1353
Size: 1005 62 4341
Fragments arranged by size:
4341 1005 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 1109 1369 1475 2275 2290 3233 3665 4412
Size: 391 260 106 800 15 943 432 747
Cuts at: 4412 4680 718
Size: 268 1446
Fragments arranged by size:
1446 943 800 747 432 391 268 260
106 15
EcoNI CCTnn'n_nnAGG
Cuts at: 568 628 1091 2257 568
Size: 60 463 1166 3719
Fragments arranged by size:
3719 1166 463 60
EcoO109I rG'GnC_Cy
Cuts at: 266 1798 1964 2102 2207 2547 266
Size: 1532 166 138 105 340 3127
Fragments arranged by size:
3127 1532 340 166 138 105
EcoRI G'AATT_C
Cuts at: 2412 2412
Size: 5408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 861 3427 861
Size: 2566 2842
FspI TGC'GCA
Cuts at: 3189 3189
Size: 5408
HaeII r_GCGC'y
Cuts at: 287 1103 1446 2115 2842 3090 4112 5091 287
Size: 816 343 669 727 248 1022 979 604
Fragments arranged by size:
1022 979 816 727 669 604 343 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 849 881 1555 1587 2199 2231 3250 3280 3282
Size: 32 674 32 612 32 1019 30 2
Cuts at: 3282 3312 849
Size: 30 2945
Fragments arranged by size:
2945 1019 674 612 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1418 2235 2447 1418
Size: 817 212 4379
Fragments arranged by size:
4379 817 212
KasI G'GCGC_C
Cuts at: 1442 2838 3086 1442
Size: 1396 248 3764
Fragments arranged by size:
3764 1396 248
KpnI G_GTAC'C
Cuts at: 2428 2428
Size: 5408
MfeI C'AATT_G
Cuts at: 516 516
Size: 5408
MluI A'CGCG_T
Cuts at: 4841 4841
Size: 5408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1968 2195 2327 4079 4263 1968
Size: 227 132 1752 184 3113
Fragments arranged by size:
3113 1752 227 184 132
MscI TGG'CCA
Cuts at: 506 548 805 3169 506
Size: 42 257 2364 2745
Fragments arranged by size:
2745 2364 257 42
MslI CAynn'nnrTG
Cuts at: 418 823 1683 1780 3397 3524 418
Size: 405 860 97 1617 127 2302
Fragments arranged by size:
2302 1617 860 405 127 97
MspA1I CmG'CkG
Cuts at: 261 612 758 902 1347 1568 2519 2833 3193
Size: 351 146 144 445 221 951 314 360
Cuts at: 3193 4206 4451 261
Size: 1013 245 1218
Fragments arranged by size:
1218 1013 951 445 360 351 314 245
221 146 144
NaeI GCC'GGC
Cuts at: 651 3589 651
Size: 2938 2470
NarI GG'CG_CC
Cuts at: 1443 2839 3087 1443
Size: 1396 248 3764
Fragments arranged by size:
3764 1396 248
NcoI C'CATG_G
Cuts at: 1931 1970 1931
Size: 39 5369
NgoMIV G'CCGG_C
Cuts at: 649 3587 649
Size: 2938 2470
NsiI A_TGCA'T
Cuts at: 204 912 204
Size: 708 4700
NspI r_CATG'y
Cuts at: 1086 1560 1671 1884 2131 2367 2461 2961 3868
Size: 474 111 213 247 236 94 500 907
Cuts at: 3868 1086
Size: 2626
Fragments arranged by size:
2626 907 500 474 247 236 213 111
94
PasI CC'CwG_GG
Cuts at: 1077 1077
Size: 5408
PciI A'CATG_T
Cuts at: 1556 2363 3864 1556
Size: 807 1501 3100
Fragments arranged by size:
3100 1501 807
PflMI CCAn_nnn'nTGG
Cuts at: 476 2121 476
Size: 1645 3763
PfoI T'CCnGG_A
Cuts at: 275 1736 1960 275
Size: 1461 224 3723
Fragments arranged by size:
3723 1461 224
PmeI GTTT'AAAC
Cuts at: 2407 2407
Size: 5408
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4547 4579 4547
Size: 32 5376
PpuMI rG'GwC_Cy
Cuts at: 1964 2207 1964
Size: 243 5165
PsiI TTA'TAA
Cuts at: 1511 1511
Size: 5408
PspOMI G'GGCC_C
Cuts at: 1797 1797
Size: 5408
PstI C_TGCA'G
Cuts at: 1185 1598 2147 2179 2387 2455 3140 1185
Size: 413 549 32 208 68 685 3453
Fragments arranged by size:
3453 685 549 413 208 68 32
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5408
PvuII CAG'CTG
Cuts at: 261 612 758 1347 2833 3193 261
Size: 351 146 589 1486 360 2476
Fragments arranged by size:
2476 1486 589 360 351 146
RsrII CG'GwC_CG
Cuts at: 1212 3603 1212
Size: 2391 3017
SacI G_AGCT'C
Cuts at: 1355 2360 2422 1355
Size: 1005 62 4341
Fragments arranged by size:
4341 1005 62
SalI G'TCGA_C
Cuts at: 1416 2445 1416
Size: 1029 4379
SapI GCTCTTCn'nnn_
Cuts at: 142 445 1304 3431 3641 142
Size: 303 859 2127 210 1909
Fragments arranged by size:
2127 1909 859 303 210
SbfI CC_TGCA'GG
Cuts at: 2455 2455
Size: 5408
ScaI AGT'ACT
Cuts at: 4925 4925
Size: 5408
SexAI A'CCwGG_T
Cuts at: 1838 1838
Size: 5408
SfcI C'TryA_G
Cuts at: 33 1181 1594 2143 2175 2383 2451 3136 4129
Size: 1148 413 549 32 208 68 685 993
Cuts at: 4129 4320 33
Size: 191 1121
Fragments arranged by size:
1148 1121 993 685 549 413 208 191
68 32
SfoI GGC'GCC
Cuts at: 1444 2840 3088 1444
Size: 1396 248 3764
Fragments arranged by size:
3764 1396 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5408
SmaI CCC'GGG
Cuts at: 2245 2430 2245
Size: 185 5223
SmlI C'TyrA_G
Cuts at: 211 1361 1501 2563 3478 3970 4232 211
Size: 1150 140 1062 915 492 262 1387
Fragments arranged by size:
1387 1150 1062 915 492 262 140
SnaBI TAC'GTA
Cuts at: 2400 2400
Size: 5408
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5408
SphI G_CATG'C
Cuts at: 1086 1884 2131 2461 2961 1086
Size: 798 247 330 500 3533
Fragments arranged by size:
3533 798 500 330 247
StuI AGG'CCT
Cuts at: 556 556
Size: 5408
StyI C'CwwG_G
Cuts at: 1931 1970 2087 2093 2542 2896 1931
Size: 39 117 6 449 354 4443
Fragments arranged by size:
4443 449 354 117 39 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1337 1924 2991 3124 3583 3792 1337
Size: 587 1067 133 459 209 2953
Fragments arranged by size:
2953 1067 587 459 209 133
TatI w'GTAC_w
Cuts at: 929 1452 4923 929
Size: 523 3471 1414
Fragments arranged by size:
3471 1414 523
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1982 2014 4925 4957 1982
Size: 32 2911 32 2433
Fragments arranged by size:
2911 2433 32 32
Tth111I GACn'n_nGTC
Cuts at: 1547 1840 3205 1547
Size: 293 1365 3750
Fragments arranged by size:
3750 1365 293
XbaI T'CTAG_A
Cuts at: 2439 2439
Size: 5408
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 978 1968 2903 978
Size: 990 935 3483
Fragments arranged by size:
3483 990 935
XmaI C'CCGG_G
Cuts at: 2243 2428 2243
Size: 185 5223
XmnI GAAnn'nnTTC
Cuts at: 347 1115 1789 347
Size: 768 674 3966
Fragments arranged by size:
3966 768 674
ZraI GAC'GTC
Cuts at: 357 2238 357
Size: 1881 3527
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AhdI AloI AscI AvrII BglI BmgBI BmtI
BsiWI BsrGI BssHII BstZ17I Bsu36I EcoRV FseI FspAI
HindIII HpaI NdeI NheI NotI NruI PacI PmlI
PshAI PspXI PsrI SacII SanDI SfiI SgrAI SrfI
SspI SwaI XhoI