Restriction map for
pF1KSDA0205
(Circular) (Six-base) MAPSORT of: pF1KSDA0205.seq Check: 862 from: 1 to: 4247
pF1KSDA0205 4247 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1285 1285
Size: 4247
Acc65I G'GTAC_C
Cuts at: 1263 1263
Size: 4247
AclI AA'CG_TT
Cuts at: 4087 4087
Size: 4247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 2072 2504 3251 3519 238
Size: 1834 432 747 268 966
Fragments arranged by size:
1834 966 747 432 268
AflIII A'CryG_T
Cuts at: 966 1599 2703 3680 966
Size: 633 1104 977 1533
Fragments arranged by size:
1533 1104 977 633
AgeI A'CCGG_T
Cuts at: 2635 2635
Size: 4247
AlwNI CAG_nnn'CTG
Cuts at: 606 1552 3119 606
Size: 946 1567 1734
Fragments arranged by size:
1734 1567 946
ApaLI G'TGCA_C
Cuts at: 447 3017 447
Size: 2570 1677
ApoI r'AATT_y
Cuts at: 1251 3600 3856 1251
Size: 2349 256 1642
Fragments arranged by size:
2349 1642 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4247
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
AvaI C'yCGr_G
Cuts at: 1267 1267
Size: 4247
BamHI G'GATC_C
Cuts at: 935 1272 935
Size: 337 3910
BanI G'GyrC_C
Cuts at: 1263 1677 1925 1960 1263
Size: 414 248 35 3550
Fragments arranged by size:
3550 414 248 35
BanII G_rGCy'C
Cuts at: 1261 2291 1261
Size: 1030 3217
BbeI G_GCGC'C
Cuts at: 1681 1929 1681
Size: 248 3999
BbsI GAAGACnn'nnnn_
Cuts at: 1064 1064
Size: 4247
BbvCI CC'TCA_GC
Cuts at: 1141 1141
Size: 4247
BciVI GTATCCnnnnn_n'
Cuts at: 937 2145 2906 4247 937
Size: 1208 761 1341 937
Fragments arranged by size:
1341 1208 937 761
BclI T'GATC_A
Cuts at: 1767 1767
Size: 4247
BglII A'GATC_T
Cuts at: 583 1762 3799 583
Size: 1179 2037 1031
Fragments arranged by size:
2037 1179 1031
BlpI GC'TnA_GC
Cuts at: 1359 1359
Size: 4247
Bme1580I G_kGCm'C
Cuts at: 451 1872 1965 3021 451
Size: 1421 93 1056 1677
Fragments arranged by size:
1677 1421 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1459 1540 1875 4019 1459
Size: 81 335 2144 1687
Fragments arranged by size:
2144 1687 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 4247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 856 1027 451
Size: 405 171 3671
Fragments arranged by size:
3671 405 171
Bpu10I CC'TnA_GC
Cuts at: 1141 1141
Size: 4247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 1423 2302 2794 3092 598
Size: 825 879 492 298 1753
Fragments arranged by size:
1753 879 825 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3951 3951
Size: 4247
BsaAI yAC'GTr
Cuts at: 1239 1600 1239
Size: 361 3886
BsaBI GATnn'nnATC
Cuts at: 6 1761 3798 6
Size: 1755 2037 455
Fragments arranged by size:
2037 1755 455
BsaHI Gr'CG_yC
Cuts at: 1678 1926 2628 4124 1678
Size: 248 702 1496 1801
Fragments arranged by size:
1801 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1436 1957 2635 2909 3056 3467 1436
Size: 521 678 274 147 411 2216
Fragments arranged by size:
2216 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 848 848
Size: 4247
BseYI C'CCAG_C
Cuts at: 156 1672 2430 3007 3503 156
Size: 1516 758 577 496 900
Fragments arranged by size:
1516 900 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1789 1835 3043 104
Size: 1685 46 1208 1308
Fragments arranged by size:
1685 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 451 1261 2039 2229 3021 451
Size: 810 778 190 792 1677
Fragments arranged by size:
1677 810 792 778 190
BsmI GAATG_Cn'
Cuts at: 798 798
Size: 4247
Bsp1286I G_dGCh'C
Cuts at: 451 1261 1872 1965 2039 2229 2291 3021 451
Size: 810 611 93 74 190 62 730 1677
Fragments arranged by size:
1677 810 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3467 3467
Size: 4247
BspHI T'CATG_A
Cuts at: 3423 3423
Size: 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1297 1511 1813 2194 1297
Size: 214 302 381 3350
Fragments arranged by size:
3350 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2539 2593 4077 4242 50
Size: 2489 54 1484 165 55
Fragments arranged by size:
2489 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1224 2159 1224
Size: 935 3312
BsrFI r'CCGG_y
Cuts at: 2245 2426 2635 2245
Size: 181 209 3857
Fragments arranged by size:
3857 209 181
BsrGI T'GTAC_A
Cuts at: 969 969
Size: 4247
BssSI C'ACGA_G
Cuts at: 2518 2876 2518
Size: 358 3889
BstAPI GCAn_nnn'nTGC
Cuts at: 1624 1624
Size: 4247
BstBI TT'CG_AA
Cuts at: 110 2608 110
Size: 2498 1749
BstYI r'GATC_y
Cuts at: 1 583 935 980 1272 1739 1762 2097 2343
Size: 582 352 45 292 467 23 335 246
Cuts at: 2343 3355 3441 3453 3799 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 582 467 449 352 346 335 292
246 86 45 23 12
Bsu36I CC'TnA_GG
Cuts at: 688 688
Size: 4247
BtgI C'CryG_G
Cuts at: 125 151 125
Size: 26 4221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2197 2378 2541 2197
Size: 181 163 3903
Fragments arranged by size:
3903 181 163
BtsI GCAGTG_nn'
Cuts at: 416 1490 416
Size: 1074 3173
DraI TTT'AAA
Cuts at: 1246 1246
Size: 4247
DrdI GACnn_nn'nnGTC
Cuts at: 1953 2811 1953
Size: 858 3389
EaeI y'GGCC_r
Cuts at: 1832 2006 2397 2424 3769 1832
Size: 174 391 27 1345 2310
Fragments arranged by size:
2310 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1832 1832
Size: 4247
EarI CTCTTCn'nnn_
Cuts at: 688 2270 2480 688
Size: 1582 210 2455
Fragments arranged by size:
2455 1582 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2455 2765 2911 4057 2455
Size: 310 146 1146 2645
Fragments arranged by size:
2645 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1259 1259
Size: 4247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 451 856 1027 2072 2504 3251 3519 238
Size: 213 405 171 1045 432 747 268 966
Fragments arranged by size:
1045 966 747 432 405 268 213 171
EcoO109I rG'GnC_Cy
Cuts at: 1386 1386
Size: 4247
EcoRI G'AATT_C
Cuts at: 1251 1251
Size: 4247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 639 2266 639
Size: 1627 2620
FspI TGC'GCA
Cuts at: 2028 2028
Size: 4247
HaeII r_GCGC'y
Cuts at: 1681 1929 2951 3930 1681
Size: 248 1022 979 1998
Fragments arranged by size:
1998 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 633 665 844 876 885 917 1051 1083 2089
Size: 32 179 32 9 32 134 32 1006
Cuts at: 2089 2119 2121 2151 633
Size: 30 2 30 2729
Fragments arranged by size:
2729 1006 179 134 32 32 32 32
30 30 9 2
HincII GTy'rAC
Cuts at: 207 1286 207
Size: 1079 3168
KasI G'GCGC_C
Cuts at: 1677 1925 1677
Size: 248 3999
KpnI G_GTAC'C
Cuts at: 1267 1267
Size: 4247
MluI A'CGCG_T
Cuts at: 3680 3680
Size: 4247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2918 3102 2918
Size: 184 4063
MscI TGG'CCA
Cuts at: 2008 2008
Size: 4247
MslI CAynn'nnrTG
Cuts at: 454 1076 2236 2363 454
Size: 622 1160 127 2338
Fragments arranged by size:
2338 1160 622 127
MspA1I CmG'CkG
Cuts at: 603 1041 1358 1672 2032 3045 3290 603
Size: 438 317 314 360 1013 245 1560
Fragments arranged by size:
1560 1013 438 360 317 314 245
NaeI GCC'GGC
Cuts at: 2428 2428
Size: 4247
NarI GG'CG_CC
Cuts at: 1678 1926 1678
Size: 248 3999
NcoI C'CATG_G
Cuts at: 125 125
Size: 4247
NgoMIV G'CCGG_C
Cuts at: 2426 2426
Size: 4247
NspI r_CATG'y
Cuts at: 970 1300 1800 2707 970
Size: 330 500 907 2510
Fragments arranged by size:
2510 907 500 330
PciI A'CATG_T
Cuts at: 966 2703 966
Size: 1737 2510
PflMI CCAn_nnn'nTGG
Cuts at: 769 769
Size: 4247
PfoI T'CCnGG_A
Cuts at: 467 467
Size: 4247
PmeI GTTT'AAAC
Cuts at: 1246 1246
Size: 4247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3386 3418 3386
Size: 32 4215
PsiI TTA'TAA
Cuts at: 571 571
Size: 4247
PstI C_TGCA'G
Cuts at: 1294 1979 1294
Size: 685 3562
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4247
PvuII CAG'CTG
Cuts at: 603 1672 2032 603
Size: 1069 360 2818
Fragments arranged by size:
2818 1069 360
RsrII CG'GwC_CG
Cuts at: 2442 2442
Size: 4247
SacI G_AGCT'C
Cuts at: 1261 1261
Size: 4247
SalI G'TCGA_C
Cuts at: 1284 1284
Size: 4247
SapI GCTCTTCn'nnn_
Cuts at: 2270 2480 2270
Size: 210 4037
SbfI CC_TGCA'GG
Cuts at: 1294 1294
Size: 4247
ScaI AGT'ACT
Cuts at: 3764 3764
Size: 4247
SexAI A'CCwGG_T
Cuts at: 212 212
Size: 4247
SfcI C'TryA_G
Cuts at: 33 917 1290 1975 2968 3159 33
Size: 884 373 685 993 191 1121
Fragments arranged by size:
1121 993 884 685 373 191
SfoI GGC'GCC
Cuts at: 1679 1927 1679
Size: 248 3999
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4247
SmaI CCC'GGG
Cuts at: 1269 1269
Size: 4247
SmlI C'TyrA_G
Cuts at: 613 1402 2317 2809 3071 613
Size: 789 915 492 262 1789
Fragments arranged by size:
1789 915 789 492 262
SnaBI TAC'GTA
Cuts at: 1239 1239
Size: 4247
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4247
SphI G_CATG'C
Cuts at: 1300 1800 1300
Size: 500 3747
StuI AGG'CCT
Cuts at: 869 869
Size: 4247
StyI C'CwwG_G
Cuts at: 125 763 939 1103 1381 1735 125
Size: 638 176 164 278 354 2637
Fragments arranged by size:
2637 638 354 278 176 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1830 1963 2422 2631 1830
Size: 133 459 209 3446
Fragments arranged by size:
3446 459 209 133
TatI w'GTAC_w
Cuts at: 524 969 3762 524
Size: 445 2793 1009
Fragments arranged by size:
2793 1009 445
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 347 379 3764 3796 347
Size: 32 3385 32 798
Fragments arranged by size:
3385 798 32 32
Tth111I GACn'n_nGTC
Cuts at: 2044 2044
Size: 4247
XbaI T'CTAG_A
Cuts at: 1278 1278
Size: 4247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1742 1742
Size: 4247
XmaI C'CCGG_G
Cuts at: 1267 1267
Size: 4247
XmnI GAAnn'nnTTC
Cuts at: 543 543
Size: 4247
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI SspI SwaI XhoI
ZraI