Restriction map for
pF1KSDA0202
(Circular) (Six-base) MAPSORT of: pF1KSDA0202.seq Check: 3096 from: 1 to: 4424
pF1KSDA0202 4424 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 906 906
Size: 4424
AccI GT'mk_AC
Cuts at: 1462 1462
Size: 4424
Acc65I G'GTAC_C
Cuts at: 1440 1440
Size: 4424
AclI AA'CG_TT
Cuts at: 4264 4264
Size: 4424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 553 637 1068 1222 1264 2249 2681 3428
Size: 316 84 431 154 42 985 432 747
Cuts at: 3428 3696 237
Size: 268 965
Fragments arranged by size:
985 965 747 432 431 316 268 154
84 42
AfeI AGC'GCT
Cuts at: 148 148
Size: 4424
AflIII A'CryG_T
Cuts at: 1776 2880 3857 1776
Size: 1104 977 2343
Fragments arranged by size:
2343 1104 977
AgeI A'CCGG_T
Cuts at: 2812 2812
Size: 4424
AlwNI CAG_nnn'CTG
Cuts at: 1386 1729 3296 1386
Size: 343 1567 2514
Fragments arranged by size:
2514 1567 343
ApaLI G'TGCA_C
Cuts at: 3194 3194
Size: 4424
ApoI r'AATT_y
Cuts at: 1428 3777 4033 1428
Size: 2349 256 1819
Fragments arranged by size:
2349 1819 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4424
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
AvaI C'yCGr_G
Cuts at: 1444 1444
Size: 4424
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 880 913 880
Size: 33 4391
BamHI G'GATC_C
Cuts at: 572 1449 572
Size: 877 3547
BanI G'GyrC_C
Cuts at: 1440 1854 2102 2137 1440
Size: 414 248 35 3727
Fragments arranged by size:
3727 414 248 35
BanII G_rGCy'C
Cuts at: 173 713 1022 1223 1438 2468 173
Size: 540 309 201 215 1030 2129
Fragments arranged by size:
2129 1030 540 309 215 201
BbeI G_GCGC'C
Cuts at: 1858 2106 1858
Size: 248 4176
BbsI GAAGACnn'nnnn_
Cuts at: 989 989
Size: 4424
BciVI GTATCCnnnnn_n'
Cuts at: 2322 3083 4424 2322
Size: 761 1341 2322
Fragments arranged by size:
2322 1341 761
BclI T'GATC_A
Cuts at: 1944 1944
Size: 4424
BglI GCCn_nnn'nGGC
Cuts at: 606 606
Size: 4424
BglII A'GATC_T
Cuts at: 627 764 1939 3976 627
Size: 137 1175 2037 1075
Fragments arranged by size:
2037 1175 1075 137
BlpI GC'TnA_GC
Cuts at: 1536 1536
Size: 4424
Bme1580I G_kGCm'C
Cuts at: 611 2049 2142 3198 611
Size: 1438 93 1056 1837
Fragments arranged by size:
1837 1438 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1636 1717 2052 4196 1636
Size: 81 335 2144 1864
Fragments arranged by size:
2144 1864 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 168 2791 168
Size: 2623 1801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 379 1216 1318 163
Size: 216 837 102 3269
Fragments arranged by size:
3269 837 216 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 1600 2479 2971 3269 400
Size: 1200 879 492 298 1555
Fragments arranged by size:
1555 1200 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 274 1300 4128 274
Size: 1026 2828 570
Fragments arranged by size:
2828 1026 570
BsaAI yAC'GTr
Cuts at: 1416 1777 1416
Size: 361 4063
BsaBI GATnn'nnATC
Cuts at: 6 730 964 1938 3975 6
Size: 724 234 974 2037 455
Fragments arranged by size:
2037 974 724 455 234
BsaHI Gr'CG_yC
Cuts at: 1855 2103 2805 4301 1855
Size: 248 702 1496 1978
Fragments arranged by size:
1978 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1613 2134 2812 3086 3233 3644 1613
Size: 521 678 274 147 411 2393
Fragments arranged by size:
2393 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 682 712 682
Size: 30 4394
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 541 871 1057 1276 1315 1318 541
Size: 330 186 219 39 3 3647
Fragments arranged by size:
3647 330 219 186 39 3
BseYI C'CCAG_C
Cuts at: 1849 2607 3184 3680 1849
Size: 758 577 496 2593
Fragments arranged by size:
2593 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 934 430
Size: 504 3920
BsiEI CG_ry'CG
Cuts at: 104 1966 2012 3220 104
Size: 1862 46 1208 1308
Fragments arranged by size:
1862 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 713 891 1022 1223 1241 1438 2216 2406 3198
Size: 178 131 201 18 197 778 190 792
Cuts at: 3198 713
Size: 1939
Fragments arranged by size:
1939 792 778 201 197 190 178 131
18
BsmI GAATG_Cn'
Cuts at: 162 825 162
Size: 663 3761
Bsp1286I G_dGCh'C
Cuts at: 173 611 713 891 1022 1223 1241 1438 2049
Size: 438 102 178 131 201 18 197 611
Cuts at: 2049 2142 2216 2406 2468 3198 173
Size: 93 74 190 62 730 1399
Fragments arranged by size:
1399 730 611 438 201 197 190 178
131 102 93 74 62 18
BspEI T'CCGG_A
Cuts at: 3644 3644
Size: 4424
BspHI T'CATG_A
Cuts at: 815 3600 815
Size: 2785 1639
BspMI ACCTGCnnnn'nnnn_
Cuts at: 906 1474 1688 1990 2371 906
Size: 568 214 302 381 2959
Fragments arranged by size:
2959 568 381 302 214
BsrBI CCG'CTC
Cuts at: 50 545 2716 2770 4254 4419 50
Size: 495 2171 54 1484 165 55
Fragments arranged by size:
2171 1484 495 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2336 2336
Size: 4424
BsrFI r'CCGG_y
Cuts at: 283 1007 1025 2422 2603 2812 283
Size: 724 18 1397 181 209 1895
Fragments arranged by size:
1895 1397 724 209 181 18
BssSI C'ACGA_G
Cuts at: 2695 3053 2695
Size: 358 4066
BstAPI GCAn_nnn'nTGC
Cuts at: 1362 1801 1362
Size: 439 3985
BstBI TT'CG_AA
Cuts at: 110 2785 110
Size: 2675 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 141 141
Size: 4424
BstYI r'GATC_y
Cuts at: 1 536 572 627 764 1449 1916 1939 2274
Size: 535 36 55 137 685 467 23 335
Cuts at: 2274 2520 3532 3618 3630 3976 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 685 535 467 449 346 335 246
137 86 55 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 1387 1387
Size: 4424
BtgI C'CryG_G
Cuts at: 125 437 779 839 125
Size: 312 342 60 3710
Fragments arranged by size:
3710 342 312 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 663 1079 2374 2555 2718 663
Size: 416 1295 181 163 2369
Fragments arranged by size:
2369 1295 416 181 163
BtsI GCAGTG_nn'
Cuts at: 933 1667 933
Size: 734 3690
ClaI AT'CG_AT
Cuts at: 497 497
Size: 4424
DraI TTT'AAA
Cuts at: 1423 1423
Size: 4424
DrdI GACnn_nn'nnGTC
Cuts at: 226 2130 2988 226
Size: 1904 858 1662
Fragments arranged by size:
1904 1662 858
EaeI y'GGCC_r
Cuts at: 131 192 2009 2183 2574 2601 3946 131
Size: 61 1817 174 391 27 1345 609
Fragments arranged by size:
1817 1345 609 391 174 61 27
EagI C'GGCC_G
Cuts at: 2009 2009
Size: 4424
EarI CTCTTCn'nnn_
Cuts at: 322 556 1145 2447 2657 322
Size: 234 589 1302 210 2089
Fragments arranged by size:
2089 1302 589 234 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 529 2632 2942 3088 4234 529
Size: 2103 310 146 1146 719
Fragments arranged by size:
2103 1146 719 310 146
EcoICRI GAG'CTC
Cuts at: 711 1020 1221 1436 711
Size: 309 201 215 3699
Fragments arranged by size:
3699 309 215 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 237 379 553 637 1068 1216 1222 1264
Size: 74 142 174 84 431 148 6 42
Cuts at: 1264 1318 2249 2681 3428 3696 163
Size: 54 931 432 747 268 891
Fragments arranged by size:
931 891 747 432 431 268 174 148
142 84 74 54 42 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1390 1390
Size: 4424
EcoO109I rG'GnC_Cy
Cuts at: 1344 1563 1344
Size: 219 4205
EcoRI G'AATT_C
Cuts at: 1428 1428
Size: 4424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2443 2443
Size: 4424
FspI TGC'GCA
Cuts at: 503 2205 503
Size: 1702 2722
HaeII r_GCGC'y
Cuts at: 150 1032 1290 1858 2106 3128 4107 150
Size: 882 258 568 248 1022 979 467
Fragments arranged by size:
1022 979 882 568 467 258 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 682 714 2266 2296 2298 2328 682
Size: 32 1552 30 2 30 2778
Fragments arranged by size:
2778 1552 32 30 30 2
HincII GTy'rAC
Cuts at: 489 1173 1463 489
Size: 684 290 3450
Fragments arranged by size:
3450 684 290
KasI G'GCGC_C
Cuts at: 1854 2102 1854
Size: 248 4176
KpnI G_GTAC'C
Cuts at: 1444 1444
Size: 4424
MluI A'CGCG_T
Cuts at: 3857 3857
Size: 4424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1019 3095 3279 1019
Size: 2076 184 2164
Fragments arranged by size:
2164 2076 184
MscI TGG'CCA
Cuts at: 2185 2185
Size: 4424
MslI CAynn'nnrTG
Cuts at: 304 2413 2540 304
Size: 2109 127 2188
Fragments arranged by size:
2188 2109 127
MspA1I CmG'CkG
Cuts at: 225 1535 1849 2209 3222 3467 225
Size: 1310 314 360 1013 245 1182
Fragments arranged by size:
1310 1182 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1009 2605 1009
Size: 1596 2828
NarI GG'CG_CC
Cuts at: 1855 2103 1855
Size: 248 4176
NcoI C'CATG_G
Cuts at: 125 125
Size: 4424
NgoMIV G'CCGG_C
Cuts at: 1007 2603 1007
Size: 1596 2828
NspI r_CATG'y
Cuts at: 365 1477 1977 2884 365
Size: 1112 500 907 1905
Fragments arranged by size:
1905 1112 907 500
PasI CC'CwG_GG
Cuts at: 186 902 186
Size: 716 3708
PciI A'CATG_T
Cuts at: 2880 2880
Size: 4424
PfoI T'CCnGG_A
Cuts at: 395 395
Size: 4424
PmeI GTTT'AAAC
Cuts at: 1423 1423
Size: 4424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 3563
Size: 32 4392
PstI C_TGCA'G
Cuts at: 524 1139 1352 1471 2156 524
Size: 615 213 119 685 2792
Fragments arranged by size:
2792 685 615 213 119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4424
PvuII CAG'CTG
Cuts at: 225 1849 2209 225
Size: 1624 360 2440
Fragments arranged by size:
2440 1624 360
RsrII CG'GwC_CG
Cuts at: 2619 2619
Size: 4424
SacI G_AGCT'C
Cuts at: 713 1022 1223 1438 713
Size: 309 201 215 3699
Fragments arranged by size:
3699 309 215 201
SalI G'TCGA_C
Cuts at: 1461 1461
Size: 4424
SapI GCTCTTCn'nnn_
Cuts at: 556 2447 2657 556
Size: 1891 210 2323
Fragments arranged by size:
2323 1891 210
SbfI CC_TGCA'GG
Cuts at: 1471 1471
Size: 4424
ScaI AGT'ACT
Cuts at: 3941 3941
Size: 4424
SfcI C'TryA_G
Cuts at: 33 520 1135 1348 1467 2152 3145 3336 33
Size: 487 615 213 119 685 993 191 1121
Fragments arranged by size:
1121 993 685 615 487 213 191 119
SfoI GGC'GCC
Cuts at: 1856 2104 1856
Size: 248 4176
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4424
SmaI CCC'GGG
Cuts at: 1446 1446
Size: 4424
SmlI C'TyrA_G
Cuts at: 415 1579 2494 2986 3248 415
Size: 1164 915 492 262 1591
Fragments arranged by size:
1591 1164 915 492 262
SnaBI TAC'GTA
Cuts at: 1416 1416
Size: 4424
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4424
SphI G_CATG'C
Cuts at: 365 1477 1977 365
Size: 1112 500 2812
Fragments arranged by size:
2812 1112 500
StuI AGG'CCT
Cuts at: 1360 1360
Size: 4424
StyI C'CwwG_G
Cuts at: 125 683 1558 1912 125
Size: 558 875 354 2637
Fragments arranged by size:
2637 875 558 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 228 2007 2140 2599 2808 228
Size: 1779 133 459 209 1844
Fragments arranged by size:
1844 1779 459 209 133
TatI w'GTAC_w
Cuts at: 3939 3939
Size: 4424
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3941 3973 3941
Size: 32 4392
Tth111I GACn'n_nGTC
Cuts at: 2221 2221
Size: 4424
XbaI T'CTAG_A
Cuts at: 624 1455 624
Size: 831 3593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1919 1919
Size: 4424
XmaI C'CCGG_G
Cuts at: 1444 1444
Size: 4424
XmnI GAAnn'nnTTC
Cuts at: 1120 1120
Size: 4424
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI SwaI XhoI ZraI