Restriction map for
pF1KSDA0201
(Circular) (Six-base) MAPSORT of: pF1KSDA0201.seq Check: 8811 from: 1 to: 5711
pF1KSDA0201 5711 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2749 2749
Size: 5711
Acc65I G'GTAC_C
Cuts at: 2727 2727
Size: 5711
AclI AA'CG_TT
Cuts at: 5551 5551
Size: 5711
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 1385 1676 2162 2273 2307 3536 3968 4715
Size: 865 291 486 111 34 1229 432 747
Cuts at: 4715 4983 520
Size: 268 1248
Fragments arranged by size:
1248 1229 865 747 486 432 291 268
111 34
AflIII A'CryG_T
Cuts at: 1961 2509 3063 4167 5144 1961
Size: 548 554 1104 977 2528
Fragments arranged by size:
2528 1104 977 554 548
AgeI A'CCGG_T
Cuts at: 221 4099 221
Size: 3878 1833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2318 2318
Size: 5711
AlwNI CAG_nnn'CTG
Cuts at: 577 1705 1834 2140 2292 3016 4583 577
Size: 1128 129 306 152 724 1567 1705
Fragments arranged by size:
1705 1567 1128 724 306 152 129
ApaLI G'TGCA_C
Cuts at: 225 4481 225
Size: 4256 1455
ApoI r'AATT_y
Cuts at: 671 865 1163 1192 1297 2089 2115 2676 2715
Size: 194 298 29 105 792 26 561 39
Cuts at: 2715 5064 5320 671
Size: 2349 256 1062
Fragments arranged by size:
2349 1062 792 561 298 256 194 105
39 29 26
AseI AT'TA_AT
Cuts at: 20 2209 20
Size: 2189 3522
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
AvaI C'yCGr_G
Cuts at: 179 2731 179
Size: 2552 3159
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 907 940 907
Size: 33 5678
BamHI G'GATC_C
Cuts at: 2454 2736 2454
Size: 282 5429
BanI G'GyrC_C
Cuts at: 2727 3141 3389 3424 2727
Size: 414 248 35 5014
Fragments arranged by size:
5014 414 248 35
BanII G_rGCy'C
Cuts at: 154 961 2725 3755 154
Size: 807 1764 1030 2110
Fragments arranged by size:
2110 1764 1030 807
BbeI G_GCGC'C
Cuts at: 3145 3393 3145
Size: 248 5463
BbsI GAAGACnn'nnnn_
Cuts at: 1643 2150 2618 1643
Size: 507 468 4736
Fragments arranged by size:
4736 507 468
BciVI GTATCCnnnnn_n'
Cuts at: 3609 4370 5711 3609
Size: 761 1341 3609
Fragments arranged by size:
3609 1341 761
BclI T'GATC_A
Cuts at: 2448 3231 2448
Size: 783 4928
BglII A'GATC_T
Cuts at: 3226 5263 3226
Size: 2037 3674
BlpI GC'TnA_GC
Cuts at: 2823 2823
Size: 5711
Bme1580I G_kGCm'C
Cuts at: 229 3336 3429 4485 229
Size: 3107 93 1056 1455
Fragments arranged by size:
3107 1455 1056 93
BmgBI CAC'GTC
Cuts at: 147 147
Size: 5711
BmrI ACTGGGnnnn_n'
Cuts at: 802 2923 3004 3339 5483 802
Size: 2121 81 335 2144 1030
Fragments arranged by size:
2144 2121 1030 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4078 4078
Size: 5711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1853 2210 1853
Size: 357 5354
Bpu10I CC'TnA_GC
Cuts at: 2429 2471 2429
Size: 42 5669
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 508 2887 3766 4258 4556 405
Size: 103 2379 879 492 298 1560
Fragments arranged by size:
2379 1560 879 492 298 103
BsaI GGTCTCn'nnnn_
Cuts at: 190 1677 5415 190
Size: 1487 3738 486
Fragments arranged by size:
3738 1487 486
BsaAI yAC'GTr
Cuts at: 2467 2703 3064 2467
Size: 236 361 5114
Fragments arranged by size:
5114 361 236
BsaBI GATnn'nnATC
Cuts at: 6 2453 3225 5262 6
Size: 2447 772 2037 455
Fragments arranged by size:
2447 2037 772 455
BsaHI Gr'CG_yC
Cuts at: 3142 3390 4092 5588 3142
Size: 248 702 1496 3265
Fragments arranged by size:
3265 1496 702 248
BsaWI w'CCGG_w
Cuts at: 221 1018 2900 3421 4099 4373 4520 4931 221
Size: 797 1882 521 678 274 147 411 1001
Fragments arranged by size:
1882 1001 797 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 896 926 896
Size: 30 5681
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1651 2119 1651
Size: 468 5243
BseYI C'CCAG_C
Cuts at: 3136 3894 4471 4967 3136
Size: 758 577 496 3880
Fragments arranged by size:
3880 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 881 1154 1514 587
Size: 294 273 360 4784
Fragments arranged by size:
4784 360 294 273
BsiEI CG_ry'CG
Cuts at: 104 222 3253 3299 4507 104
Size: 118 3031 46 1208 1308
Fragments arranged by size:
3031 1308 1208 118 46
BsiHKAI G_wGCw'C
Cuts at: 229 912 961 2472 2725 3503 3693 4485 229
Size: 683 49 1511 253 778 190 792 1455
Fragments arranged by size:
1511 1455 792 778 683 253 190 49
BsmI GAATG_Cn'
Cuts at: 346 997 2430 346
Size: 651 1433 3627
Fragments arranged by size:
3627 1433 651
Bsp1286I G_dGCh'C
Cuts at: 154 229 912 961 2472 2725 3336 3429 3503
Size: 75 683 49 1511 253 611 93 74
Cuts at: 3503 3693 3755 4485 154
Size: 190 62 730 1380
Fragments arranged by size:
1511 1380 730 683 611 253 190 93
75 74 62 49
BspEI T'CCGG_A
Cuts at: 1018 4931 1018
Size: 3913 1798
BspHI T'CATG_A
Cuts at: 1374 2420 4887 1374
Size: 1046 2467 2198
Fragments arranged by size:
2467 2198 1046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2761 2975 3277 3658 2761
Size: 214 302 381 4814
Fragments arranged by size:
4814 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4003 4057 5541 5706 50
Size: 3953 54 1484 165 55
Fragments arranged by size:
3953 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3623 3623
Size: 5711
BsrFI r'CCGG_y
Cuts at: 221 3709 3890 4099 221
Size: 3488 181 209 1833
Fragments arranged by size:
3488 1833 209 181
BsrGI T'GTAC_A
Cuts at: 437 437
Size: 5711
BssSI C'ACGA_G
Cuts at: 3982 4340 3982
Size: 358 5353
BstAPI GCAn_nnn'nTGC
Cuts at: 3088 3088
Size: 5711
BstBI TT'CG_AA
Cuts at: 110 4072 110
Size: 3962 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 132 132
Size: 5711
BstYI r'GATC_y
Cuts at: 1 684 2454 2736 3203 3226 3561 3807 4819
Size: 683 1770 282 467 23 335 246 1012
Cuts at: 4819 4905 4917 5263 1
Size: 86 12 346 449
Fragments arranged by size:
1770 1012 683 467 449 346 335 282
246 86 23 12
BtgI C'CryG_G
Cuts at: 1584 1584
Size: 5711
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 153 186 3661 3842 4005 153
Size: 33 3475 181 163 1859
Fragments arranged by size:
3475 1859 181 163 33
BtsI GCAGTG_nn'
Cuts at: 2954 2954
Size: 5711
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2055 2090 2055
Size: 35 5676
DraI TTT'AAA
Cuts at: 870 1287 2710 870
Size: 417 1423 3871
Fragments arranged by size:
3871 1423 417
DrdI GACnn_nn'nnGTC
Cuts at: 3417 4275 3417
Size: 858 4853
EaeI y'GGCC_r
Cuts at: 339 2260 3296 3470 3861 3888 5233 339
Size: 1921 1036 174 391 27 1345 817
Fragments arranged by size:
1921 1345 1036 817 391 174 27
EagI C'GGCC_G
Cuts at: 3296 3296
Size: 5711
EarI CTCTTCn'nnn_
Cuts at: 3734 3944 3734
Size: 210 5501
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3919 4229 4375 5521 3919
Size: 310 146 1146 4109
Fragments arranged by size:
4109 1146 310 146
EcoICRI GAG'CTC
Cuts at: 959 2723 959
Size: 1764 3947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 1385 1676 1853 2162 2210 2273 2307 3536
Size: 865 291 177 309 48 63 34 1229
Cuts at: 3536 3968 4715 4983 520
Size: 432 747 268 1248
Fragments arranged by size:
1248 1229 865 747 432 309 291 268
177 63 48 34
EcoNI CCTnn'n_nnAGG
Cuts at: 815 815
Size: 5711
EcoO109I rG'GnC_Cy
Cuts at: 1438 2850 1438
Size: 1412 4299
EcoRI G'AATT_C
Cuts at: 1163 2715 1163
Size: 1552 4159
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 399 1102 1457 1972 3730 399
Size: 703 355 515 1758 2380
Fragments arranged by size:
2380 1758 703 515 355
FspI TGC'GCA
Cuts at: 3492 3492
Size: 5711
HaeII r_GCGC'y
Cuts at: 1157 3145 3393 4415 5394 1157
Size: 1988 248 1022 979 1474
Fragments arranged by size:
1988 1474 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 571 603 880 912 1547 1579 2433
Size: 32 403 32 277 32 635 32 854
Cuts at: 2433 2465 3553 3583 3585 3615 136
Size: 32 1088 30 2 30 2232
Fragments arranged by size:
2232 1088 854 635 403 277 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 491 1578 1860 2193 2750 491
Size: 1087 282 333 557 3452
Fragments arranged by size:
3452 1087 557 333 282
HindIII A'AGCT_T
Cuts at: 1454 1454
Size: 5711
KasI G'GCGC_C
Cuts at: 3141 3389 3141
Size: 248 5463
KpnI G_GTAC'C
Cuts at: 2731 2731
Size: 5711
MluI A'CGCG_T
Cuts at: 5144 5144
Size: 5711
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 122 402 866 1079 1128 2180 4382 4566 122
Size: 280 464 213 49 1052 2202 184 1267
Fragments arranged by size:
2202 1267 1052 464 280 213 184 49
MscI TGG'CCA
Cuts at: 3472 3472
Size: 5711
MslI CAynn'nnrTG
Cuts at: 130 857 1260 2354 2648 3700 3827 130
Size: 727 403 1094 294 1052 127 2014
Fragments arranged by size:
2014 1094 1052 727 403 294 127
MspA1I CmG'CkG
Cuts at: 652 1171 2317 2822 3136 3496 4509 4754 652
Size: 519 1146 505 314 360 1013 245 1609
Fragments arranged by size:
1609 1146 1013 519 505 360 314 245
NaeI GCC'GGC
Cuts at: 3892 3892
Size: 5711
NarI GG'CG_CC
Cuts at: 3142 3390 3142
Size: 248 5463
NcoI C'CATG_G
Cuts at: 1584 1584
Size: 5711
NdeI CA'TA_TG
Cuts at: 2082 2187 2082
Size: 105 5606
NgoMIV G'CCGG_C
Cuts at: 3890 3890
Size: 5711
NspI r_CATG'y
Cuts at: 1965 2513 2764 3264 4171 1965
Size: 548 251 500 907 3505
Fragments arranged by size:
3505 907 548 500 251
PciI A'CATG_T
Cuts at: 1961 2509 4167 1961
Size: 548 1658 3505
Fragments arranged by size:
3505 1658 548
PmeI GTTT'AAAC
Cuts at: 2710 2710
Size: 5711
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4850 4882 4850
Size: 32 5679
PsiI TTA'TAA
Cuts at: 677 677
Size: 5711
PstI C_TGCA'G
Cuts at: 2297 2758 3443 2297
Size: 461 685 4565
Fragments arranged by size:
4565 685 461
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5711
PvuII CAG'CTG
Cuts at: 652 1171 2317 3136 3496 652
Size: 519 1146 819 360 2867
Fragments arranged by size:
2867 1146 819 519 360
RsrII CG'GwC_CG
Cuts at: 3906 3906
Size: 5711
SacI G_AGCT'C
Cuts at: 961 2725 961
Size: 1764 3947
SalI G'TCGA_C
Cuts at: 2748 2748
Size: 5711
SapI GCTCTTCn'nnn_
Cuts at: 3734 3944 3734
Size: 210 5501
SbfI CC_TGCA'GG
Cuts at: 2758 2758
Size: 5711
ScaI AGT'ACT
Cuts at: 5228 5228
Size: 5711
SfcI C'TryA_G
Cuts at: 33 2293 2754 3439 4432 4623 33
Size: 2260 461 685 993 191 1121
Fragments arranged by size:
2260 1121 993 685 461 191
SfoI GGC'GCC
Cuts at: 3143 3391 3143
Size: 248 5463
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
SmaI CCC'GGG
Cuts at: 181 2733 181
Size: 2552 3159
SmlI C'TyrA_G
Cuts at: 384 523 2866 3781 4273 4535 384
Size: 139 2343 915 492 262 1560
Fragments arranged by size:
2343 1560 915 492 262 139
SnaBI TAC'GTA
Cuts at: 2703 2703
Size: 5711
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5711
SphI G_CATG'C
Cuts at: 2764 3264 2764
Size: 500 5211
SrfI GCCC'GGGC
Cuts at: 181 181
Size: 5711
SspI AAT'ATT
Cuts at: 2586 2586
Size: 5711
StyI C'CwwG_G
Cuts at: 1473 1584 2845 3199 1473
Size: 111 1261 354 3985
Fragments arranged by size:
3985 1261 354 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 433 1597 2568 3294 3427 3886 4095 433
Size: 1164 971 726 133 459 209 2049
Fragments arranged by size:
2049 1164 971 726 459 209 133
TatI w'GTAC_w
Cuts at: 437 878 5226 437
Size: 441 4348 922
Fragments arranged by size:
4348 922 441
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 960 992 5228 5260 960
Size: 32 4236 32 1411
Fragments arranged by size:
4236 1411 32 32
Tth111I GACn'n_nGTC
Cuts at: 3508 3508
Size: 5711
XbaI T'CTAG_A
Cuts at: 1552 2742 1552
Size: 1190 4521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 1893 3206 132
Size: 1761 1313 2637
Fragments arranged by size:
2637 1761 1313
XmaI C'CCGG_G
Cuts at: 179 2731 179
Size: 2552 3159
XmnI GAAnn'nnTTC
Cuts at: 1370 1370
Size: 5711
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmtI BsiWI
BsmBI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI StuI
SwaI XhoI ZraI