Restriction map for
pF1KSDA0190
(Circular) (Six-base) MAPSORT of: pF1KSDA0190.seq Check: 3986 from: 1 to: 5546
pF1KSDA0190 5546 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AatII G_ACGT'C
Cuts at: 2402 2402
Size: 5546
AccI GT'mk_AC
Cuts at: 1698 2354 2584 1698
Size: 656 230 4660
Fragments arranged by size:
4660 656 230
Acc65I G'GTAC_C
Cuts at: 964 2562 964
Size: 1598 3948
AclI AA'CG_TT
Cuts at: 5386 5386
Size: 5546
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 3371 3803 4550 4818 206
Size: 3165 432 747 268 934
Fragments arranged by size:
3165 934 747 432 268
AflIII A'CryG_T
Cuts at: 2898 4002 4979 2898
Size: 1104 977 3465
Fragments arranged by size:
3465 1104 977
AgeI A'CCGG_T
Cuts at: 3934 3934
Size: 5546
AhdI GACnn_n'nnGTC
Cuts at: 930 930
Size: 5546
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1891 1923 1891
Size: 32 5514
AlwNI CAG_nnn'CTG
Cuts at: 499 793 916 2851 4418 499
Size: 294 123 1935 1567 1627
Fragments arranged by size:
1935 1627 1567 294 123
ApaI G_GGCC'C
Cuts at: 877 1894 877
Size: 1017 4529
ApaLI G'TGCA_C
Cuts at: 4316 4316
Size: 5546
ApoI r'AATT_y
Cuts at: 190 406 724 2313 2550 4899 5155 190
Size: 216 318 1589 237 2349 256 581
Fragments arranged by size:
2349 1589 581 318 256 237 216
AseI AT'TA_AT
Cuts at: 20 1418 20
Size: 1398 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5546
AvaI C'yCGr_G
Cuts at: 877 1017 1090 2566 877
Size: 140 73 1476 3857
Fragments arranged by size:
3857 1476 140 73
BamHI G'GATC_C
Cuts at: 1308 2571 1308
Size: 1263 4283
BanI G'GyrC_C
Cuts at: 964 1135 2228 2562 2976 3224 3259 964
Size: 171 1093 334 414 248 35 3251
Fragments arranged by size:
3251 1093 414 334 248 171 35
BanII G_rGCy'C
Cuts at: 877 951 1126 1643 1894 2560 3590 877
Size: 74 175 517 251 666 1030 2833
Fragments arranged by size:
2833 1030 666 517 251 175 74
BbeI G_GCGC'C
Cuts at: 2980 3228 2980
Size: 248 5298
BbsI GAAGACnn'nnnn_
Cuts at: 2224 2394 2224
Size: 170 5376
BbvCI CC'TCA_GC
Cuts at: 1712 1712
Size: 5546
BciVI GTATCCnnnnn_n'
Cuts at: 3444 4205 5546 3444
Size: 761 1341 3444
Fragments arranged by size:
3444 1341 761
BclI T'GATC_A
Cuts at: 1391 2456 3066 1391
Size: 1065 610 3871
Fragments arranged by size:
3871 1065 610
BglII A'GATC_T
Cuts at: 3061 5098 3061
Size: 2037 3509
BlpI GC'TnA_GC
Cuts at: 950 2658 950
Size: 1708 3838
Bme1580I G_kGCm'C
Cuts at: 877 1030 1894 3171 3264 4320 877
Size: 153 864 1277 93 1056 2103
Fragments arranged by size:
2103 1277 1056 864 153 93
BmrI ACTGGGnnnn_n'
Cuts at: 335 2758 2839 3174 5318 335
Size: 2423 81 335 2144 563
Fragments arranged by size:
2423 2144 563 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3913 3913
Size: 5546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1360 1646 2101 2279 1360
Size: 286 455 178 4627
Fragments arranged by size:
4627 455 286 178
Bpu10I CC'TnA_GC
Cuts at: 1712 1712
Size: 5546
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2722 3601 4093 4391 2722
Size: 879 492 298 3877
Fragments arranged by size:
3877 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5250 5250
Size: 5546
BsaAI yAC'GTr
Cuts at: 2538 2899 2538
Size: 361 5185
BsaBI GATnn'nnATC
Cuts at: 6 3060 5097 6
Size: 3054 2037 455
Fragments arranged by size:
3054 2037 455
BsaHI Gr'CG_yC
Cuts at: 2399 2977 3225 3927 5423 2399
Size: 578 248 702 1496 2522
Fragments arranged by size:
2522 1496 702 578 248
BsaWI w'CCGG_w
Cuts at: 1296 2735 3256 3934 4208 4355 4766 1296
Size: 1439 521 678 274 147 411 2076
Fragments arranged by size:
2076 1439 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 203 1191 203
Size: 988 4558
BseYI C'CCAG_C
Cuts at: 364 943 2971 3729 4306 4802 364
Size: 579 2028 758 577 496 1108
Fragments arranged by size:
2028 1108 758 579 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 2176 731
Size: 1445 4101
BsiEI CG_ry'CG
Cuts at: 104 3088 3134 4342 104
Size: 2984 46 1208 1308
Fragments arranged by size:
2984 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 834 2560 3338 3528 4320 834
Size: 1726 778 190 792 2060
Fragments arranged by size:
2060 1726 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 834 877 951 1030 1126 1643 1894 2560 3171
Size: 43 74 79 96 517 251 666 611
Cuts at: 3171 3264 3338 3528 3590 4320 834
Size: 93 74 190 62 730 2060
Fragments arranged by size:
2060 730 666 611 517 251 190 96
93 79 74 74 62 43
BspEI T'CCGG_A
Cuts at: 4766 4766
Size: 5546
BspHI T'CATG_A
Cuts at: 1179 1746 4722 1179
Size: 567 2976 2003
Fragments arranged by size:
2976 2003 567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1260 2537 2596 2810 3112 3493 1260
Size: 1277 59 214 302 381 3313
Fragments arranged by size:
3313 1277 381 302 214 59
BsrBI CCG'CTC
Cuts at: 50 3838 3892 5376 5541 50
Size: 3788 54 1484 165 55
Fragments arranged by size:
3788 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3458 3458
Size: 5546
BsrFI r'CCGG_y
Cuts at: 1206 1948 3544 3725 3934 1206
Size: 742 1596 181 209 2818
Fragments arranged by size:
2818 1596 742 209 181
BsrGI T'GTAC_A
Cuts at: 421 421
Size: 5546
BssSI C'ACGA_G
Cuts at: 2185 3817 4175 2185
Size: 1632 358 3556
Fragments arranged by size:
3556 1632 358
BstAPI GCAn_nnn'nTGC
Cuts at: 718 1441 2923 718
Size: 723 1482 3341
Fragments arranged by size:
3341 1482 723
BstBI TT'CG_AA
Cuts at: 110 3907 110
Size: 3797 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1044 1044
Size: 5546
BstYI r'GATC_y
Cuts at: 1 1308 2571 3038 3061 3396 3642 4654 4740
Size: 1307 1263 467 23 335 246 1012 86
Cuts at: 4740 4752 5098 1
Size: 12 346 449
Fragments arranged by size:
1307 1263 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 395 395
Size: 5546
BtgI C'CryG_G
Cuts at: 140 1058 1383 140
Size: 918 325 4303
Fragments arranged by size:
4303 918 325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 656 3496 3677 3840 656
Size: 2840 181 163 2362
Fragments arranged by size:
2840 2362 181 163
BtsI GCAGTG_nn'
Cuts at: 846 1426 2354 2789 846
Size: 580 928 435 3603
Fragments arranged by size:
3603 928 580 435
ClaI AT'CG_AT
Cuts at: 2435 2435
Size: 5546
DraI TTT'AAA
Cuts at: 2319 2545 2319
Size: 226 5320
DrdI GACnn_nn'nnGTC
Cuts at: 2446 3252 4110 2446
Size: 806 858 3882
Fragments arranged by size:
3882 858 806
EaeI y'GGCC_r
Cuts at: 604 675 2382 3131 3305 3696 3723 5068 604
Size: 71 1707 749 174 391 27 1345 1082
Fragments arranged by size:
1707 1345 1082 749 391 174 71 27
EagI C'GGCC_G
Cuts at: 3131 3131
Size: 5546
EarI CTCTTCn'nnn_
Cuts at: 1200 1835 2148 3569 3779 1200
Size: 635 313 1421 210 2967
Fragments arranged by size:
2967 1421 635 313 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 550 1939 3754 4064 4210 5356 550
Size: 1389 1815 310 146 1146 740
Fragments arranged by size:
1815 1389 1146 740 310 146
EcoICRI GAG'CTC
Cuts at: 2558 2558
Size: 5546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 1360 1646 2101 2279 3371 3803 4550 4818
Size: 1154 286 455 178 1092 432 747 268
Cuts at: 4818 206
Size: 934
Fragments arranged by size:
1154 1092 934 747 455 432 286 268
178
EcoO109I rG'GnC_Cy
Cuts at: 399 873 874 1891 2685 399
Size: 474 1 1017 794 3260
Fragments arranged by size:
3260 1017 794 474 1
EcoRI G'AATT_C
Cuts at: 190 2550 190
Size: 2360 3186
EcoRV GAT'ATC
Cuts at: 2043 2043
Size: 5546
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1306 2305 3565 1306
Size: 999 1260 3287
Fragments arranged by size:
3287 1260 999
FspI TGC'GCA
Cuts at: 2431 3327 2431
Size: 896 4650
HaeII r_GCGC'y
Cuts at: 2980 3228 4250 5229 2980
Size: 248 1022 979 3297
Fragments arranged by size:
3297 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 544 576 637 669 916 948 1084 1116 1589
Size: 32 61 32 247 32 136 32 473
Cuts at: 1589 1621 2032 2064 2090 2122 3388 3418 3420
Size: 32 411 32 26 32 1266 30 2
Cuts at: 3420 3450 544
Size: 30 2640
Fragments arranged by size:
2640 1266 473 411 247 136 61 32
32 32 32 32 32 32 30 30
26 2
HincII GTy'rAC
Cuts at: 1514 1671 1699 2585 1514
Size: 157 28 886 4475
Fragments arranged by size:
4475 886 157 28
HindIII A'AGCT_T
Cuts at: 1771 2236 1771
Size: 465 5081
HpaI GTT'AAC
Cuts at: 1671 1671
Size: 5546
KasI G'GCGC_C
Cuts at: 2976 3224 2976
Size: 248 5298
KpnI G_GTAC'C
Cuts at: 968 2566 968
Size: 1598 3948
MluI A'CGCG_T
Cuts at: 4979 4979
Size: 5546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1832 2018 4217 4401 1832
Size: 186 2199 184 2977
Fragments arranged by size:
2977 2199 186 184
MscI TGG'CCA
Cuts at: 677 3307 677
Size: 2630 2916
MslI CAynn'nnrTG
Cuts at: 1561 3535 3662 1561
Size: 1974 127 3445
Fragments arranged by size:
3445 1974 127
MspA1I CmG'CkG
Cuts at: 943 2657 2971 3331 4344 4589 943
Size: 1714 314 360 1013 245 1900
Fragments arranged by size:
1900 1714 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3727 3727
Size: 5546
NarI GG'CG_CC
Cuts at: 2977 3225 2977
Size: 248 5298
NcoI C'CATG_G
Cuts at: 140 140
Size: 5546
NgoMIV G'CCGG_C
Cuts at: 3725 3725
Size: 5546
NspI r_CATG'y
Cuts at: 741 2599 3099 4006 741
Size: 1858 500 907 2281
Fragments arranged by size:
2281 1858 907 500
PciI A'CATG_T
Cuts at: 4002 4002
Size: 5546
PflMI CCAn_nnn'nTGG
Cuts at: 244 1163 244
Size: 919 4627
PfoI T'CCnGG_A
Cuts at: 2069 2069
Size: 5546
PmeI GTTT'AAAC
Cuts at: 2545 2545
Size: 5546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4685 4717 4685
Size: 32 5514
PshAI GACnn'nnGTC
Cuts at: 1909 1909
Size: 5546
PspOMI G'GGCC_C
Cuts at: 873 1890 873
Size: 1017 4529
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1396 1428 1396
Size: 32 5514
PstI C_TGCA'G
Cuts at: 858 912 1433 2593 3278 858
Size: 54 521 1160 685 3126
Fragments arranged by size:
3126 1160 685 521 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5546
PvuII CAG'CTG
Cuts at: 943 2971 3331 943
Size: 2028 360 3158
Fragments arranged by size:
3158 2028 360
RsrII CG'GwC_CG
Cuts at: 3741 3741
Size: 5546
SacI G_AGCT'C
Cuts at: 2560 2560
Size: 5546
SalI G'TCGA_C
Cuts at: 1697 2583 1697
Size: 886 4660
SapI GCTCTTCn'nnn_
Cuts at: 1835 3569 3779 1835
Size: 1734 210 3602
Fragments arranged by size:
3602 1734 210
SbfI CC_TGCA'GG
Cuts at: 2593 2593
Size: 5546
ScaI AGT'ACT
Cuts at: 5063 5063
Size: 5546
SexAI A'CCwGG_T
Cuts at: 2468 2468
Size: 5546
SfcI C'TryA_G
Cuts at: 33 369 854 908 1313 1429 2393 2589 3274
Size: 336 485 54 405 116 964 196 685
Cuts at: 3274 4267 4458 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 964 685 485 405 336 196
191 116 54
SfoI GGC'GCC
Cuts at: 2978 3226 2978
Size: 248 5298
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5546
SgrAI Cr'CCGG_yG
Cuts at: 1206 1206
Size: 5546
SmaI CCC'GGG
Cuts at: 879 2568 879
Size: 1689 3857
SmlI C'TyrA_G
Cuts at: 2701 3616 4108 4370 2701
Size: 915 492 262 3877
Fragments arranged by size:
3877 915 492 262
SnaBI TAC'GTA
Cuts at: 2538 2538
Size: 5546
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5546
SphI G_CATG'C
Cuts at: 2599 3099 2599
Size: 500 5046
SspI AAT'ATT
Cuts at: 2250 2250
Size: 5546
StuI AGG'CCT
Cuts at: 1503 1503
Size: 5546
StyI C'CwwG_G
Cuts at: 140 984 2109 2680 3034 140
Size: 844 1125 571 354 2652
Fragments arranged by size:
2652 1125 844 571 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 721 1533 1818 2216 2401 3129 3262 3721 3930
Size: 812 285 398 185 728 133 459 209
Cuts at: 3930 721
Size: 2337
Fragments arranged by size:
2337 812 728 459 398 285 209 185
133
TatI w'GTAC_w
Cuts at: 421 5061 421
Size: 4640 906
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5063 5095 5063
Size: 32 5514
Tth111I GACn'n_nGTC
Cuts at: 793 3343 793
Size: 2550 2996
XbaI T'CTAG_A
Cuts at: 2577 2577
Size: 5546
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 663 1251 3041 663
Size: 588 1790 3168
Fragments arranged by size:
3168 1790 588
XmaI C'CCGG_G
Cuts at: 877 2566 877
Size: 1689 3857
XmnI GAAnn'nnTTC
Cuts at: 410 1552 2203 410
Size: 1142 651 3753
Fragments arranged by size:
3753 1142 651
ZraI GAC'GTC
Cuts at: 2400 2400
Size: 5546
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PshAI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AscI AvrII BaeI
BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BssHII BstEII BstZ17I CspCI DraIII EcoNI FseI
FspAI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PsiI PspXI SacII SanDI SfiI
SrfI SwaI XhoI