Restriction map for
pF1KSDA0186
(Circular) (Six-base) MAPSORT of: pF1KSDA0186.seq Check: 6550 from: 1 to: 3725
pF1KSDA0186 3725 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 763 763
Size: 3725
Acc65I G'GTAC_C
Cuts at: 741 741
Size: 3725
AclI AA'CG_TT
Cuts at: 3565 3565
Size: 3725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 605 1550 1982 2729 2997 470
Size: 135 945 432 747 268 1198
Fragments arranged by size:
1198 945 747 432 268 135
AflIII A'CryG_T
Cuts at: 1077 2181 3158 1077
Size: 1104 977 1644
Fragments arranged by size:
1644 1104 977
AgeI A'CCGG_T
Cuts at: 2113 2113
Size: 3725
AhdI GACnn_n'nnGTC
Cuts at: 691 691
Size: 3725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 206 206
Size: 3725
AlwNI CAG_nnn'CTG
Cuts at: 1030 2597 1030
Size: 1567 2158
ApaLI G'TGCA_C
Cuts at: 2495 2495
Size: 3725
ApoI r'AATT_y
Cuts at: 313 598 729 3078 3334 313
Size: 285 131 2349 256 704
Fragments arranged by size:
2349 704 285 256 131
AseI AT'TA_AT
Cuts at: 20 20
Size: 3725
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
AvaI C'yCGr_G
Cuts at: 745 745
Size: 3725
BamHI G'GATC_C
Cuts at: 750 750
Size: 3725
BanI G'GyrC_C
Cuts at: 741 1155 1403 1438 741
Size: 414 248 35 3028
Fragments arranged by size:
3028 414 248 35
BanII G_rGCy'C
Cuts at: 739 1769 739
Size: 1030 2695
BbeI G_GCGC'C
Cuts at: 1159 1407 1159
Size: 248 3477
BciVI GTATCCnnnnn_n'
Cuts at: 1623 2384 3725 1623
Size: 761 1341 1623
Fragments arranged by size:
1623 1341 761
BclI T'GATC_A
Cuts at: 680 1245 680
Size: 565 3160
BfrBI ATG'CAT
Cuts at: 427 427
Size: 3725
BglI GCCn_nnn'nGGC
Cuts at: 180 180
Size: 3725
BglII A'GATC_T
Cuts at: 481 1240 3277 481
Size: 759 2037 929
Fragments arranged by size:
2037 929 759
BlpI GC'TnA_GC
Cuts at: 837 837
Size: 3725
Bme1580I G_kGCm'C
Cuts at: 1350 1443 2499 1350
Size: 93 1056 2576
Fragments arranged by size:
2576 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 517 937 1018 1353 3497 517
Size: 420 81 335 2144 745
Fragments arranged by size:
2144 745 420 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 3725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 718 244
Size: 474 3251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 1780 2272 2570 901
Size: 879 492 298 2056
Fragments arranged by size:
2056 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3429 3429
Size: 3725
BsaAI yAC'GTr
Cuts at: 717 1078 717
Size: 361 3364
BsaBI GATnn'nnATC
Cuts at: 6 1239 3276 6
Size: 1233 2037 455
Fragments arranged by size:
2037 1233 455
BsaHI Gr'CG_yC
Cuts at: 1156 1404 2106 3602 1156
Size: 248 702 1496 1279
Fragments arranged by size:
1496 1279 702 248
BsaWI w'CCGG_w
Cuts at: 575 914 1435 2113 2387 2534 2945 575
Size: 339 521 678 274 147 411 1355
Fragments arranged by size:
1355 678 521 411 339 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 241 527 241
Size: 286 3439
BseYI C'CCAG_C
Cuts at: 1150 1908 2485 2981 1150
Size: 758 577 496 1894
Fragments arranged by size:
1894 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 333
Size: 3725
BsiEI CG_ry'CG
Cuts at: 104 1267 1313 2521 104
Size: 1163 46 1208 1308
Fragments arranged by size:
1308 1208 1163 46
BsiHKAI G_wGCw'C
Cuts at: 393 704 739 1517 1707 2499 393
Size: 311 35 778 190 792 1619
Fragments arranged by size:
1619 792 778 311 190 35
Bsp1286I G_dGCh'C
Cuts at: 393 704 739 1350 1443 1517 1707 1769 2499
Size: 311 35 611 93 74 190 62 730
Cuts at: 2499 393
Size: 1619
Fragments arranged by size:
1619 730 611 311 190 93 74 62
35
BspEI T'CCGG_A
Cuts at: 2945 2945
Size: 3725
BspHI T'CATG_A
Cuts at: 2901 2901
Size: 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 775 989 1291 1672 775
Size: 214 302 381 2828
Fragments arranged by size:
2828 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2017 2071 3555 3720 50
Size: 1967 54 1484 165 55
Fragments arranged by size:
1967 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1637 1637
Size: 3725
BsrFI r'CCGG_y
Cuts at: 1723 1904 2113 1723
Size: 181 209 3335
Fragments arranged by size:
3335 209 181
BssHII G'CGCG_C
Cuts at: 170 170
Size: 3725
BssSI C'ACGA_G
Cuts at: 1996 2354 1996
Size: 358 3367
BstAPI GCAn_nnn'nTGC
Cuts at: 1102 1102
Size: 3725
BstBI TT'CG_AA
Cuts at: 110 2086 110
Size: 1976 1749
BstYI r'GATC_y
Cuts at: 1 481 750 1217 1240 1575 1821 2833 2919
Size: 480 269 467 23 335 246 1012 86
Cuts at: 2919 2931 3277 1
Size: 12 346 449
Fragments arranged by size:
1012 480 467 449 346 335 269 246
86 23 12
BtgI C'CryG_G
Cuts at: 143 143
Size: 3725
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 151 1675 1856 2019 151
Size: 1524 181 163 1857
Fragments arranged by size:
1857 1524 181 163
BtsI GCAGTG_nn'
Cuts at: 968 968
Size: 3725
DraI TTT'AAA
Cuts at: 724 724
Size: 3725
DrdI GACnn_nn'nnGTC
Cuts at: 1431 2289 1431
Size: 858 2867
EaeI y'GGCC_r
Cuts at: 1310 1484 1875 1902 3247 1310
Size: 174 391 27 1345 1788
Fragments arranged by size:
1788 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1310 1310
Size: 3725
EarI CTCTTCn'nnn_
Cuts at: 1748 1958 1748
Size: 210 3515
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1933 2243 2389 3535 1933
Size: 310 146 1146 2123
Fragments arranged by size:
2123 1146 310 146
EcoICRI GAG'CTC
Cuts at: 737 737
Size: 3725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 470 605 718 1550 1982 2729 2997 244
Size: 226 135 113 832 432 747 268 972
Fragments arranged by size:
972 832 747 432 268 226 135 113
EcoO109I rG'GnC_Cy
Cuts at: 864 864
Size: 3725
EcoRI G'AATT_C
Cuts at: 729 729
Size: 3725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1744 1744
Size: 3725
FspI TGC'GCA
Cuts at: 1506 1506
Size: 3725
HaeII r_GCGC'y
Cuts at: 1159 1407 2429 3408 1159
Size: 248 1022 979 1476
Fragments arranged by size:
1476 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 677 709 1567 1597 1599 1629 677
Size: 32 858 30 2 30 2773
Fragments arranged by size:
2773 858 32 30 30 2
HincII GTy'rAC
Cuts at: 764 764
Size: 3725
HindIII A'AGCT_T
Cuts at: 236 236
Size: 3725
KasI G'GCGC_C
Cuts at: 1155 1403 1155
Size: 248 3477
KpnI G_GTAC'C
Cuts at: 745 745
Size: 3725
MluI A'CGCG_T
Cuts at: 3158 3158
Size: 3725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2396 2580 2396
Size: 184 3541
MscI TGG'CCA
Cuts at: 1486 1486
Size: 3725
MslI CAynn'nnrTG
Cuts at: 396 1714 1841 396
Size: 1318 127 2280
Fragments arranged by size:
2280 1318 127
MspA1I CmG'CkG
Cuts at: 678 836 1150 1510 2523 2768 678
Size: 158 314 360 1013 245 1635
Fragments arranged by size:
1635 1013 360 314 245 158
NaeI GCC'GGC
Cuts at: 1906 1906
Size: 3725
NarI GG'CG_CC
Cuts at: 1156 1404 1156
Size: 248 3477
NcoI C'CATG_G
Cuts at: 143 143
Size: 3725
NgoMIV G'CCGG_C
Cuts at: 1904 1904
Size: 3725
NsiI A_TGCA'T
Cuts at: 429 429
Size: 3725
NspI r_CATG'y
Cuts at: 778 1278 2185 778
Size: 500 907 2318
Fragments arranged by size:
2318 907 500
PciI A'CATG_T
Cuts at: 2181 2181
Size: 3725
PfoI T'CCnGG_A
Cuts at: 695 695
Size: 3725
PmeI GTTT'AAAC
Cuts at: 724 724
Size: 3725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2864 2896 2864
Size: 32 3693
PsiI TTA'TAA
Cuts at: 476 476
Size: 3725
PstI C_TGCA'G
Cuts at: 772 1457 772
Size: 685 3040
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3725
PvuII CAG'CTG
Cuts at: 678 1150 1510 678
Size: 472 360 2893
Fragments arranged by size:
2893 472 360
RsrII CG'GwC_CG
Cuts at: 1920 1920
Size: 3725
SacI G_AGCT'C
Cuts at: 739 739
Size: 3725
SalI G'TCGA_C
Cuts at: 762 762
Size: 3725
SapI GCTCTTCn'nnn_
Cuts at: 1748 1958 1748
Size: 210 3515
SbfI CC_TGCA'GG
Cuts at: 772 772
Size: 3725
ScaI AGT'ACT
Cuts at: 3242 3242
Size: 3725
SfcI C'TryA_G
Cuts at: 33 353 768 1453 2446 2637 33
Size: 320 415 685 993 191 1121
Fragments arranged by size:
1121 993 685 415 320 191
SfoI GGC'GCC
Cuts at: 1157 1405 1157
Size: 248 3477
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
SmaI CCC'GGG
Cuts at: 747 747
Size: 3725
SmlI C'TyrA_G
Cuts at: 880 1795 2287 2549 880
Size: 915 492 262 2056
Fragments arranged by size:
2056 915 492 262
SnaBI TAC'GTA
Cuts at: 717 717
Size: 3725
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3725
SphI G_CATG'C
Cuts at: 778 1278 778
Size: 500 3225
StyI C'CwwG_G
Cuts at: 143 859 1213 143
Size: 716 354 2655
Fragments arranged by size:
2655 716 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1308 1441 1900 2109 1308
Size: 133 459 209 2924
Fragments arranged by size:
2924 459 209 133
TatI w'GTAC_w
Cuts at: 3240 3240
Size: 3725
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 3693
Tth111I GACn'n_nGTC
Cuts at: 1522 1522
Size: 3725
XbaI T'CTAG_A
Cuts at: 756 756
Size: 3725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1220 1220
Size: 3725
XmaI C'CCGG_G
Cuts at: 745 745
Size: 3725
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI ZraI