Restriction map for
pF1KSDA0181
(Circular) (Six-base) MAPSORT of: pF1KSDA0181.seq Check: 1714 from: 1 to: 9326
pF1KSDA0181 9326 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1789 1827 1789
Size: 38 9288
AccI GT'mk_AC
Cuts at: 5313 6364 5313
Size: 1051 8275
Acc65I G'GTAC_C
Cuts at: 5889 6342 5889
Size: 453 8873
AclI AA'CG_TT
Cuts at: 4835 9166 4835
Size: 4331 4995
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3058 3545 3812 4508 4934 5702 6206 6236 7151
Size: 487 267 696 426 768 504 30 915
Cuts at: 7151 7583 8330 8598 3058
Size: 432 747 268 3786
Fragments arranged by size:
3786 915 768 747 696 504 487 432
426 268 267 30
AflIII A'CryG_T
Cuts at: 3582 6678 7782 8759 3582
Size: 3096 1104 977 4149
Fragments arranged by size:
4149 3096 1104 977
AgeI A'CCGG_T
Cuts at: 7714 7714
Size: 9326
AhdI GACnn_n'nnGTC
Cuts at: 5320 5522 5320
Size: 202 9124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3395 3395
Size: 9326
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3301 3333 3301
Size: 32 9294
AlwNI CAG_nnn'CTG
Cuts at: 2424 2487 4459 4734 6631 8198 2424
Size: 63 1972 275 1897 1567 3552
Fragments arranged by size:
3552 1972 1897 1567 275 63
ApaI G_GGCC'C
Cuts at: 1358 1975 2085 2141 2191 2504 3304 5812 1358
Size: 617 110 56 50 313 800 2508 4872
Fragments arranged by size:
4872 2508 800 617 313 110 56 50
ApaLI G'TGCA_C
Cuts at: 8096 8096
Size: 9326
ApoI r'AATT_y
Cuts at: 594 1028 1752 1883 3019 3684 3918 4056 4167
Size: 434 724 131 1136 665 234 138 111
Cuts at: 4167 4323 4410 4557 5245 5600 6030 6330 8679
Size: 156 87 147 688 355 430 300 2349
Cuts at: 8679 8935 594
Size: 256 985
Fragments arranged by size:
2349 1136 985 724 688 665 434 430
355 300 256 234 156 147 138 131
111 87
AseI AT'TA_AT
Cuts at: 20 3893 20
Size: 3873 5453
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9326
AvaI C'yCGr_G
Cuts at: 428 681 2121 2649 5811 6346 428
Size: 253 1440 528 3162 535 3408
Fragments arranged by size:
3408 3162 1440 535 528 253
AvrII C'CTAG_G
Cuts at: 1647 3910 1647
Size: 2263 7063
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5873 5906 5873
Size: 33 9293
BamHI G'GATC_C
Cuts at: 452 777 1438 3249 4521 5989 6351 452
Size: 325 661 1811 1272 1468 362 3427
Fragments arranged by size:
3427 1811 1468 1272 661 362 325
BanI G'GyrC_C
Cuts at: 711 1059 1722 2202 3108 5889 6342 6756 7004
Size: 348 663 480 906 2781 453 414 248
Cuts at: 7004 7039 711
Size: 35 2998
Fragments arranged by size:
2998 2781 906 663 480 453 414 348
248 35
BanII G_rGCy'C
Cuts at: 395 797 1101 1358 1764 1975 2085 2141 2191
Size: 402 304 257 406 211 110 56 50
Cuts at: 2191 2504 2706 2779 3304 3478 4626 5717 5812
Size: 313 202 73 525 174 1148 1091 95
Cuts at: 5812 5903 6134 6340 7370 395
Size: 91 231 206 1030 2351
Fragments arranged by size:
2351 1148 1091 1030 525 406 402 313
304 257 231 211 206 202 174 110
95 91 73 56 50
BbeI G_GCGC'C
Cuts at: 2206 6760 7008 2206
Size: 4554 248 4524
Fragments arranged by size:
4554 4524 248
BbsI GAAGACnn'nnnn_
Cuts at: 161 197 4730 5366 161
Size: 36 4533 636 4121
Fragments arranged by size:
4533 4121 636 36
BbvCI CC'TCA_GC
Cuts at: 1766 1942 3155 5457 5823 1766
Size: 176 1213 2302 366 5269
Fragments arranged by size:
5269 2302 1213 366 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1692 1726 4574 4608 1692
Size: 34 2848 34 6410
Fragments arranged by size:
6410 2848 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7224 7985 9326 7224
Size: 761 1341 7224
Fragments arranged by size:
7224 1341 761
BclI T'GATC_A
Cuts at: 2955 3936 6846 2955
Size: 981 2910 5435
Fragments arranged by size:
5435 2910 981
BglI GCCn_nnn'nGGC
Cuts at: 2232 2499 5807 2232
Size: 267 3308 5751
Fragments arranged by size:
5751 3308 267
BglII A'GATC_T
Cuts at: 2910 4659 6841 8878 2910
Size: 1749 2182 2037 3358
Fragments arranged by size:
3358 2182 2037 1749
BlpI GC'TnA_GC
Cuts at: 6438 6438
Size: 9326
Bme1580I G_kGCm'C
Cuts at: 344 1135 1358 1975 2015 2085 2141 2191 2504
Size: 791 223 617 40 70 56 50 313
Cuts at: 2504 3304 5812 5818 6951 7044 8100 344
Size: 800 2508 6 1133 93 1056 1570
Fragments arranged by size:
2508 1570 1133 1056 800 791 617 313
223 93 70 56 50 40 6
BmrI ACTGGGnnnn_n'
Cuts at: 1032 2743 3173 4532 5548 6538 6619 6954 9098
Size: 1711 430 1359 1016 990 81 335 2144
Cuts at: 9098 1032
Size: 1260
Fragments arranged by size:
2144 1711 1359 1260 1016 990 430 335
81
BmtI G_CTAG'C
Cuts at: 5441 5441
Size: 9326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3531 5922 7693 3531
Size: 2391 1771 5164
Fragments arranged by size:
5164 2391 1771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1055 1097 1115 1180 2993 4100 4640 4661 4838
Size: 42 18 65 1813 1107 540 21 177
Cuts at: 4838 5265 5363 6112 6243 1055
Size: 427 98 749 131 4138
Fragments arranged by size:
4138 1813 1107 749 540 427 177 131
98 65 42 21 18
Bpu10I CC'TnA_GC
Cuts at: 1212 1766 1942 3155 5326 5457 5823 1212
Size: 554 176 1213 2171 131 366 4715
Fragments arranged by size:
4715 2171 1213 554 366 176 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1916 3638 5662 6502 7381 7873 8171 1916
Size: 1722 2024 840 879 492 298 3071
Fragments arranged by size:
3071 2024 1722 879 840 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 9030 9030
Size: 9326
BsaAI yAC'GTr
Cuts at: 6318 6679 6318
Size: 361 8965
BsaBI GATnn'nnATC
Cuts at: 6 6840 8877 6
Size: 6834 2037 455
Fragments arranged by size:
6834 2037 455
BsaHI Gr'CG_yC
Cuts at: 1228 2203 6757 7005 7707 9203 1228
Size: 975 4554 248 702 1496 1351
Fragments arranged by size:
4554 1496 1351 975 702 248
BsaWI w'CCGG_w
Cuts at: 6515 7036 7714 7988 8135 8546 6515
Size: 521 678 274 147 411 7295
Fragments arranged by size:
7295 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 3301 3331 5361 5391 5691 5721 1178
Size: 30 2093 30 2030 30 300 30 4783
Fragments arranged by size:
4783 2093 2030 300 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 737 775 1100 1319 1404 1413 1811 3077 4253
Size: 38 325 219 85 9 398 1266 1176
Cuts at: 4253 5992 737
Size: 1739 4071
Fragments arranged by size:
4071 1739 1266 1176 398 325 219 85
38 9
BseYI C'CCAG_C
Cuts at: 885 899 1870 1964 2084 2180 2429 2503 2519
Size: 14 971 94 120 96 249 74 16
Cuts at: 2519 2934 3668 3694 3814 5769 5833 6010 6751
Size: 415 734 26 120 1955 64 177 741
Cuts at: 6751 7509 8086 8582 885
Size: 758 577 496 1629
Fragments arranged by size:
1955 1629 971 758 741 734 577 496
415 249 177 120 120 96 94 74
64 26 16 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2413 2644 5425 5687 2413
Size: 231 2781 262 6052
Fragments arranged by size:
6052 2781 262 231
BsiEI CG_ry'CG
Cuts at: 104 6868 6914 8122 104
Size: 6764 46 1208 1308
Fragments arranged by size:
6764 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2706 4626 5853 5903 6340 7118 7308 8100 2706
Size: 1920 1227 50 437 778 190 792 3932
Fragments arranged by size:
3932 1920 1227 792 778 437 190 50
BsmI GAATG_Cn'
Cuts at: 1661 2471 2591 3332 3419 1661
Size: 810 120 741 87 7568
Fragments arranged by size:
7568 810 741 120 87
Bsp1286I G_dGCh'C
Cuts at: 344 395 797 1101 1135 1358 1764 1975 2015
Size: 51 402 304 34 223 406 211 40
Cuts at: 2015 2085 2141 2191 2504 2706 2779 3304 3478
Size: 70 56 50 313 202 73 525 174
Cuts at: 3478 4626 5717 5812 5818 5853 5903 6134 6340
Size: 1148 1091 95 6 35 50 231 206
Cuts at: 6340 6951 7044 7118 7308 7370 8100 344
Size: 611 93 74 190 62 730 1570
Fragments arranged by size:
1570 1148 1091 730 611 525 406 402
313 304 231 223 211 206 202 190
174 95 93 74 73 70 62 56
51 50 50 40 35 34 6
BspEI T'CCGG_A
Cuts at: 8546 8546
Size: 9326
BspHI T'CATG_A
Cuts at: 3236 8502 3236
Size: 5266 4060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1789 1827 2226 3387 4106 6255 6376 6590 6892
Size: 38 399 1161 719 2149 121 214 302
Cuts at: 6892 7273 1789
Size: 381 3842
Fragments arranged by size:
3842 2149 1161 719 399 381 302 214
121 38
BsrBI CCG'CTC
Cuts at: 50 444 7618 7672 9156 9321 50
Size: 394 7174 54 1484 165 55
Fragments arranged by size:
7174 1484 394 165 55 54
BsrDI GCAATG_nn'
Cuts at: 779 7238 779
Size: 6459 2867
BsrFI r'CCGG_y
Cuts at: 3029 5543 7324 7505 7714 3029
Size: 2514 1781 181 209 4641
Fragments arranged by size:
4641 2514 1781 209 181
BssSI C'ACGA_G
Cuts at: 5333 7597 7955 5333
Size: 2264 358 6704
Fragments arranged by size:
6704 2264 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2010 2241 2631 4459 6703 2010
Size: 231 390 1828 2244 4633
Fragments arranged by size:
4633 2244 1828 390 231
BstBI TT'CG_AA
Cuts at: 110 7687 110
Size: 7577 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 132 1878 2134 2527 4266 5745 132
Size: 1746 256 393 1739 1479 3713
Fragments arranged by size:
3713 1746 1739 1479 393 256
BstYI r'GATC_y
Cuts at: 1 452 777 826 1438 2910 3249 4521 4659
Size: 451 325 49 612 1472 339 1272 138
Cuts at: 4659 4731 5989 6351 6818 6841 7176 7422 8434
Size: 72 1258 362 467 23 335 246 1012
Cuts at: 8434 8520 8532 8878 1
Size: 86 12 346 449
Fragments arranged by size:
1472 1272 1258 1012 612 467 451 449
362 346 339 335 325 246 138 86
72 49 23 12
Bsu36I CC'TnA_GG
Cuts at: 822 2228 4391 5444 822
Size: 1406 2163 1053 4704
Fragments arranged by size:
4704 2163 1406 1053
BtgI C'CryG_G
Cuts at: 125 581 1217 1845 2670 125
Size: 456 636 628 825 6781
Fragments arranged by size:
6781 825 636 628 456
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3620 7276 7457 7620 3620
Size: 3656 181 163 5326
Fragments arranged by size:
5326 3656 181 163
BtsI GCAGTG_nn'
Cuts at: 3354 3543 4723 5816 6061 6132 6569 3354
Size: 189 1180 1093 245 71 437 6111
Fragments arranged by size:
6111 1180 1093 437 245 189 71
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 252 254 287 289 5919 5954 252
Size: 2 33 2 5630 35 3624
Fragments arranged by size:
5630 3624 35 33 2 2
DraI TTT'AAA
Cuts at: 3923 6325 3923
Size: 2402 6924
DrdI GACnn_nn'nnGTC
Cuts at: 7032 7890 7032
Size: 858 8468
EaeI y'GGCC_r
Cuts at: 2491 3031 3705 4268 5595 6911 7085 7476 7503
Size: 540 674 563 1327 1316 174 391 27
Cuts at: 7503 8848 2491
Size: 1345 2969
Fragments arranged by size:
2969 1345 1327 1316 674 563 540 391
174 27
EagI C'GGCC_G
Cuts at: 6911 6911
Size: 9326
EarI CTCTTCn'nnn_
Cuts at: 1333 2942 3883 4541 4884 5480 5487 6182 7349
Size: 1609 941 658 343 596 7 695 1167
Cuts at: 7349 7559 1333
Size: 210 3100
Fragments arranged by size:
3100 1609 1167 941 695 658 596 343
210 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3520 5898 7534 7844 7990 9136 3520
Size: 2378 1636 310 146 1146 3710
Fragments arranged by size:
3710 2378 1636 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2704 4624 5901 6338 2704
Size: 1920 1277 437 5692
Fragments arranged by size:
5692 1920 1277 437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1055 1097 1115 1180 2993 3058 3545 3812 4100
Size: 42 18 65 1813 65 487 267 288
Cuts at: 4100 4508 4640 4661 4838 4934 5265 5363 5702
Size: 408 132 21 177 96 331 98 339
Cuts at: 5702 6112 6206 6236 6243 7151 7583 8330 8598
Size: 410 94 30 7 908 432 747 268
Cuts at: 8598 1055
Size: 1783
Fragments arranged by size:
1813 1783 908 747 487 432 410 408
339 331 288 268 267 177 132 98
96 94 65 65 42 30 21 18
7
EcoNI CCTnn'n_nnAGG
Cuts at: 5974 5974
Size: 9326
EcoO109I rG'GnC_Cy
Cuts at: 718 1354 1355 1365 1971 2081 2138 2187 2224
Size: 636 1 10 606 110 57 49 37
Cuts at: 2224 2437 2484 2500 3004 3300 3301 3376 4695
Size: 213 47 16 504 296 1 75 1319
Cuts at: 4695 5531 5830 6465 718
Size: 836 299 635 3579
Fragments arranged by size:
3579 1319 836 636 635 606 504 299
296 213 110 75 57 49 47 37
16 10 1 1
EcoRI G'AATT_C
Cuts at: 1028 1752 1883 4056 4323 4410 6330 1028
Size: 724 131 2173 267 87 1920 4024
Fragments arranged by size:
4024 2173 1920 724 267 131 87
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1185 4395 7345 1185
Size: 3210 2950 3166
Fragments arranged by size:
3210 3166 2950
FspI TGC'GCA
Cuts at: 7107 7107
Size: 9326
HaeII r_GCGC'y
Cuts at: 2206 6760 7008 8030 9009 2206
Size: 4554 248 1022 979 2523
Fragments arranged by size:
4554 2523 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 444 476 1623 1655 2115 2147 2211
Size: 32 216 32 1147 32 460 32 64
Cuts at: 2211 2243 3072 3104 3241 3273 5041 5073 5306
Size: 32 829 32 137 32 1768 32 233
Cuts at: 5306 5338 5508 5540 5719 5751 7168 7198 7200
Size: 32 170 32 179 32 1417 30 2
Cuts at: 7200 7230 196
Size: 30 2292
Fragments arranged by size:
2292 1768 1417 1147 829 460 233 216
179 170 137 64 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1464 2790 4603 5003 5314 5492 5609 6365 1464
Size: 1326 1813 400 311 178 117 756 4425
Fragments arranged by size:
4425 1813 1326 756 400 311 178 117
HpaI GTT'AAC
Cuts at: 1464 1464
Size: 9326
KasI G'GCGC_C
Cuts at: 2202 6756 7004 2202
Size: 4554 248 4524
Fragments arranged by size:
4554 4524 248
KpnI G_GTAC'C
Cuts at: 5893 6346 5893
Size: 453 8873
MluI A'CGCG_T
Cuts at: 8759 8759
Size: 9326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1531 2271 3979 7997 8181 1531
Size: 740 1708 4018 184 2676
Fragments arranged by size:
4018 2676 1708 740 184
MscI TGG'CCA
Cuts at: 4270 5597 7087 4270
Size: 1327 1490 6509
Fragments arranged by size:
6509 1490 1327
MslI CAynn'nnrTG
Cuts at: 670 1597 1799 4781 7315 7442 670
Size: 927 202 2982 2534 127 2554
Fragments arranged by size:
2982 2554 2534 927 202 127
MspA1I CmG'CkG
Cuts at: 439 903 1086 1770 1986 2938 5062 6437 6751
Size: 464 183 684 216 952 2124 1375 314
Cuts at: 6751 7111 8124 8369 439
Size: 360 1013 245 1396
Fragments arranged by size:
2124 1396 1375 1013 952 684 464 360
314 245 216 183
NaeI GCC'GGC
Cuts at: 7507 7507
Size: 9326
NarI GG'CG_CC
Cuts at: 2203 6757 7005 2203
Size: 4554 248 4524
Fragments arranged by size:
4554 4524 248
NcoI C'CATG_G
Cuts at: 125 581 2670 125
Size: 456 2089 6781
Fragments arranged by size:
6781 2089 456
NgoMIV G'CCGG_C
Cuts at: 7505 7505
Size: 9326
NheI G'CTAG_C
Cuts at: 5437 5437
Size: 9326
NspI r_CATG'y
Cuts at: 1798 1953 1998 2379 3106 3576 3586 5621 6379
Size: 155 45 381 727 470 10 2035 758
Cuts at: 6379 6879 7786 1798
Size: 500 907 3338
Fragments arranged by size:
3338 2035 907 758 727 500 470 381
155 45 10
PasI CC'CwG_GG
Cuts at: 1119 1518 1747 2019 4156 4648 1119
Size: 399 229 272 2137 492 5797
Fragments arranged by size:
5797 2137 492 399 272 229
PciI A'CATG_T
Cuts at: 3582 7782 3582
Size: 4200 5126
PflMI CCAn_nnn'nTGG
Cuts at: 2109 2220 3628 4156 2109
Size: 111 1408 528 7279
Fragments arranged by size:
7279 1408 528 111
PfoI T'CCnGG_A
Cuts at: 636 636
Size: 9326
PmeI GTTT'AAAC
Cuts at: 6325 6325
Size: 9326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3301 3333 8465 8497 3301
Size: 32 5132 32 4130
Fragments arranged by size:
5132 4130 32 32
PpuMI rG'GwC_Cy
Cuts at: 1365 2484 3376 4695 5531 5830 1365
Size: 1119 892 1319 836 299 4861
Fragments arranged by size:
4861 1319 1119 892 836 299
PshAI GACnn'nnGTC
Cuts at: 2409 2409
Size: 9326
PspOMI G'GGCC_C
Cuts at: 1354 1971 2081 2137 2187 2500 3300 5808 1354
Size: 617 110 56 50 313 800 2508 4872
Fragments arranged by size:
4872 2508 800 617 313 110 56 50
PstI C_TGCA'G
Cuts at: 737 908 2817 4347 4730 5166 6068 6373 7058
Size: 171 1909 1530 383 436 902 305 685
Cuts at: 7058 737
Size: 3005
Fragments arranged by size:
3005 1909 1530 902 685 436 383 305
171
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9326
PvuII CAG'CTG
Cuts at: 903 1086 1770 1986 2938 5062 6751 7111 903
Size: 183 684 216 952 2124 1689 360 3118
Fragments arranged by size:
3118 2124 1689 952 684 360 216 183
RsrII CG'GwC_CG
Cuts at: 7521 7521
Size: 9326
SacI G_AGCT'C
Cuts at: 2706 4626 5903 6340 2706
Size: 1920 1277 437 5692
Fragments arranged by size:
5692 1920 1277 437
SalI G'TCGA_C
Cuts at: 5312 6363 5312
Size: 1051 8275
SanDI GG'GwC_CC
Cuts at: 1365 1365
Size: 9326
SapI GCTCTTCn'nnn_
Cuts at: 4884 6182 7349 7559 4884
Size: 1298 1167 210 6651
Fragments arranged by size:
6651 1298 1167 210
SbfI CC_TGCA'GG
Cuts at: 6373 6373
Size: 9326
ScaI AGT'ACT
Cuts at: 3590 8843 3590
Size: 5253 4073
SexAI A'CCwGG_T
Cuts at: 3036 3036
Size: 9326
SfcI C'TryA_G
Cuts at: 33 733 904 2543 2813 4343 4726 5162 6064
Size: 700 171 1639 270 1530 383 436 902
Cuts at: 6064 6369 7054 8047 8238 33
Size: 305 685 993 191 1121
Fragments arranged by size:
1639 1530 1121 993 902 700 685 436
383 305 270 191 171
SfiI GGCCn_nnn'nGGCC
Cuts at: 2499 2499
Size: 9326
SfoI GGC'GCC
Cuts at: 2204 6758 7006 2204
Size: 4554 248 4524
Fragments arranged by size:
4554 4524 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9326
SmaI CCC'GGG
Cuts at: 430 683 5813 6348 430
Size: 253 5130 535 3408
Fragments arranged by size:
5130 3408 535 253
SmlI C'TyrA_G
Cuts at: 1931 3653 5641 6481 7396 7888 8150 1931
Size: 1722 1988 840 915 492 262 3107
Fragments arranged by size:
3107 1988 1722 915 840 492 262
SnaBI TAC'GTA
Cuts at: 6318 6318
Size: 9326
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9326
SphI G_CATG'C
Cuts at: 1798 1998 6379 6879 1798
Size: 200 4381 500 4245
Fragments arranged by size:
4381 4245 500 200
SrfI GCCC'GGGC
Cuts at: 5813 5813
Size: 9326
SspI AAT'ATT
Cuts at: 329 3897 329
Size: 3568 5758
StuI AGG'CCT
Cuts at: 1646 3947 6070 1646
Size: 2301 2123 4902
Fragments arranged by size:
4902 2301 2123
StyI C'CwwG_G
Cuts at: 125 581 1149 1489 1647 2670 3295 3437 3910
Size: 456 568 340 158 1023 625 142 473
Cuts at: 3910 5612 5688 6460 6814 125
Size: 1702 76 772 354 2637
Fragments arranged by size:
2637 1702 1023 772 625 568 473 456
354 340 158 142 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 863 2178 3813 5790 6909 7042 7501 7710 863
Size: 1315 1635 1977 1119 133 459 209 2479
Fragments arranged by size:
2479 1977 1635 1315 1119 459 209 133
TatI w'GTAC_w
Cuts at: 378 3588 8841 378
Size: 3210 5253 863
Fragments arranged by size:
5253 3210 863
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 346 378 1278 1310 2375 2407 3536 3568 4832
Size: 32 900 32 1065 32 1129 32 1264
Cuts at: 4832 4864 4920 4952 5691 5723 8843 8875 346
Size: 32 56 32 739 32 3120 32 797
Fragments arranged by size:
3120 1264 1129 1065 900 797 739 56
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7123 7123
Size: 9326
XbaI T'CTAG_A
Cuts at: 6357 6357
Size: 9326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 1090 1798 2511 3498 6087 6821 270
Size: 820 708 713 987 2589 734 2775
Fragments arranged by size:
2775 2589 987 820 734 713 708
XmaI C'CCGG_G
Cuts at: 428 681 5811 6346 428
Size: 253 5130 535 3408
Fragments arranged by size:
5130 3408 535 253
XmnI GAAnn'nnTTC
Cuts at: 3447 3447
Size: 9326
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AscI BfrBI BmgBI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoRV
FseI FspAI HindIII MfeI NdeI NotI NruI NsiI
PacI PmlI PsiI PspXI PsrI SacII SgrAI SwaI
XhoI ZraI