Restriction map for
pF1KSDA0180
(Circular) (Six-base) MAPSORT of: pF1KSDA0180.seq Check: 6 from: 1 to: 4301
pF1KSDA0180 4301 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1043 1142 1339 1043
Size: 99 197 4005
Fragments arranged by size:
4005 197 99
Acc65I G'GTAC_C
Cuts at: 1317 1317
Size: 4301
AclI AA'CG_TT
Cuts at: 4141 4141
Size: 4301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 618 901 1006 1141 2126 2558 3305 3573 618
Size: 283 105 135 985 432 747 268 1346
Fragments arranged by size:
1346 985 747 432 283 268 135 105
AflIII A'CryG_T
Cuts at: 1653 2757 3734 1653
Size: 1104 977 2220
Fragments arranged by size:
2220 1104 977
AgeI A'CCGG_T
Cuts at: 2689 2689
Size: 4301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 856 856
Size: 4301
AlwNI CAG_nnn'CTG
Cuts at: 1606 3173 1606
Size: 1567 2734
ApaLI G'TGCA_C
Cuts at: 3071 3071
Size: 4301
ApoI r'AATT_y
Cuts at: 1305 3654 3910 1305
Size: 2349 256 1696
Fragments arranged by size:
2349 1696 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4301
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
AvaI C'yCGr_G
Cuts at: 189 1321 189
Size: 1132 3169
BamHI G'GATC_C
Cuts at: 1326 1326
Size: 4301
BanI G'GyrC_C
Cuts at: 130 462 1317 1731 1979 2014 130
Size: 332 855 414 248 35 2417
Fragments arranged by size:
2417 855 414 332 248 35
BanII G_rGCy'C
Cuts at: 208 286 410 711 1082 1315 2345 208
Size: 78 124 301 371 233 1030 2164
Fragments arranged by size:
2164 1030 371 301 233 124 78
BbeI G_GCGC'C
Cuts at: 134 1735 1983 134
Size: 1601 248 2452
Fragments arranged by size:
2452 1601 248
BbsI GAAGACnn'nnnn_
Cuts at: 544 790 1235 544
Size: 246 445 3610
Fragments arranged by size:
3610 445 246
BciVI GTATCCnnnnn_n'
Cuts at: 2199 2960 4301 2199
Size: 761 1341 2199
Fragments arranged by size:
2199 1341 761
BclI T'GATC_A
Cuts at: 1821 1821
Size: 4301
BglI GCCn_nnn'nGGC
Cuts at: 142 142
Size: 4301
BglII A'GATC_T
Cuts at: 1816 3853 1816
Size: 2037 2264
BlpI GC'TnA_GC
Cuts at: 154 1413 154
Size: 1259 3042
Bme1580I G_kGCm'C
Cuts at: 147 547 1926 2019 3075 147
Size: 400 1379 93 1056 1373
Fragments arranged by size:
1379 1373 1056 400 93
BmgBI CAC'GTC
Cuts at: 542 542
Size: 4301
BmrI ACTGGGnnnn_n'
Cuts at: 711 1029 1513 1594 1929 4073 711
Size: 318 484 81 335 2144 939
Fragments arranged by size:
2144 939 484 335 318 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 689 2668 689
Size: 1979 2322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 424 1213 397
Size: 27 789 3485
Fragments arranged by size:
3485 789 27
Bpu10I CC'TnA_GC
Cuts at: 1076 1271 1076
Size: 195 4106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1477 2356 2848 3146 1477
Size: 879 492 298 2632
Fragments arranged by size:
2632 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4005 4005
Size: 4301
BsaAI yAC'GTr
Cuts at: 1293 1654 1293
Size: 361 3940
BsaBI GATnn'nnATC
Cuts at: 6 1815 3852 6
Size: 1809 2037 455
Fragments arranged by size:
2037 1809 455
BsaHI Gr'CG_yC
Cuts at: 131 1732 1980 2682 4178 131
Size: 1601 248 702 1496 254
Fragments arranged by size:
1601 1496 702 254 248
BsaWI w'CCGG_w
Cuts at: 687 1490 2011 2689 2963 3110 3521 687
Size: 803 521 678 274 147 411 1467
Fragments arranged by size:
1467 803 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1174 1204 1174
Size: 30 4271
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1182 1182
Size: 4301
BseYI C'CCAG_C
Cuts at: 518 711 1726 2484 3061 3557 518
Size: 193 1015 758 577 496 1262
Fragments arranged by size:
1262 1015 758 577 496 193
BsiEI CG_ry'CG
Cuts at: 104 151 221 1843 1889 3097 104
Size: 47 70 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 70 47 46
BsiHKAI G_wGCw'C
Cuts at: 1082 1315 2093 2283 3075 1082
Size: 233 778 190 792 2308
Fragments arranged by size:
2308 792 778 233 190
BsmI GAATG_Cn'
Cuts at: 833 833
Size: 4301
Bsp1286I G_dGCh'C
Cuts at: 147 208 286 410 547 711 1082 1315 1926
Size: 61 78 124 137 164 371 233 611
Cuts at: 1926 2019 2093 2283 2345 3075 147
Size: 93 74 190 62 730 1373
Fragments arranged by size:
1373 730 611 371 233 190 164 137
124 93 78 74 62 61
BspEI T'CCGG_A
Cuts at: 3521 3521
Size: 4301
BspHI T'CATG_A
Cuts at: 3477 3477
Size: 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 1351 1565 1867 2248 236
Size: 1115 214 302 381 2289
Fragments arranged by size:
2289 1115 381 302 214
BsrBI CCG'CTC
Cuts at: 50 186 1221 2593 2647 4131 4296 50
Size: 136 1035 1372 54 1484 165 55
Fragments arranged by size:
1484 1372 1035 165 136 55 54
BsrDI GCAATG_nn'
Cuts at: 2213 2213
Size: 4301
BsrFI r'CCGG_y
Cuts at: 220 2299 2480 2689 220
Size: 2079 181 209 1832
Fragments arranged by size:
2079 1832 209 181
BsrGI T'GTAC_A
Cuts at: 1148 1148
Size: 4301
BssSI C'ACGA_G
Cuts at: 2572 2930 2572
Size: 358 3943
BstAPI GCAn_nnn'nTGC
Cuts at: 1678 1678
Size: 4301
BstBI TT'CG_AA
Cuts at: 110 2662 110
Size: 2552 1749
BstEII G'GTnAC_C
Cuts at: 223 223
Size: 4301
BstXI CCAn_nnnn'nTGG
Cuts at: 1177 1177
Size: 4301
BstYI r'GATC_y
Cuts at: 1 992 1326 1793 1816 2151 2397 3409 3495
Size: 991 334 467 23 335 246 1012 86
Cuts at: 3495 3507 3853 1
Size: 12 346 449
Fragments arranged by size:
1012 991 467 449 346 335 334 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1127 1127
Size: 4301
BtgI C'CryG_G
Cuts at: 125 125
Size: 4301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2251 2432 2595 2251
Size: 181 163 3957
Fragments arranged by size:
3957 181 163
BtsI GCAGTG_nn'
Cuts at: 928 1199 1544 928
Size: 271 345 3685
Fragments arranged by size:
3685 345 271
DraI TTT'AAA
Cuts at: 1300 1300
Size: 4301
DrdI GACnn_nn'nnGTC
Cuts at: 2007 2865 2007
Size: 858 3443
EaeI y'GGCC_r
Cuts at: 148 218 1158 1886 2060 2451 2478 3823 148
Size: 70 940 728 174 391 27 1345 626
Fragments arranged by size:
1345 940 728 626 391 174 70 27
EagI C'GGCC_G
Cuts at: 148 218 1886 148
Size: 70 1668 2563
Fragments arranged by size:
2563 1668 70
EarI CTCTTCn'nnn_
Cuts at: 1096 2324 2534 1096
Size: 1228 210 2863
Fragments arranged by size:
2863 1228 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2509 2819 2965 4111 2509
Size: 310 146 1146 2699
Fragments arranged by size:
2699 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1080 1313 1080
Size: 233 4068
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 424 618 901 1006 1141 1213 2126 2558
Size: 27 194 283 105 135 72 913 432
Cuts at: 2558 3305 3573 397
Size: 747 268 1125
Fragments arranged by size:
1125 913 747 432 283 268 194 135
105 72 27
EcoO109I rG'GnC_Cy
Cuts at: 213 1224 1265 1440 213
Size: 1011 41 175 3074
Fragments arranged by size:
3074 1011 175 41
EcoRI G'AATT_C
Cuts at: 1305 1305
Size: 4301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 4301
FspI TGC'GCA
Cuts at: 844 2082 844
Size: 1238 3063
HaeII r_GCGC'y
Cuts at: 134 255 1735 1983 3005 3984 134
Size: 121 1480 248 1022 979 451
Fragments arranged by size:
1480 1022 979 451 248 121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1021 1053 2143 2173 2175 2205 1021
Size: 32 1090 30 2 30 3117
Fragments arranged by size:
3117 1090 32 30 30 2
HincII GTy'rAC
Cuts at: 1143 1340 1143
Size: 197 4104
KasI G'GCGC_C
Cuts at: 130 1731 1979 130
Size: 1601 248 2452
Fragments arranged by size:
2452 1601 248
KpnI G_GTAC'C
Cuts at: 1321 1321
Size: 4301
MluI A'CGCG_T
Cuts at: 3734 3734
Size: 4301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 590 1107 2972 3156 590
Size: 517 1865 184 1735
Fragments arranged by size:
1865 1735 517 184
MscI TGG'CCA
Cuts at: 2062 2062
Size: 4301
MslI CAynn'nnrTG
Cuts at: 2290 2417 2290
Size: 127 4174
MspA1I CmG'CkG
Cuts at: 153 949 1412 1726 2086 3099 3344 153
Size: 796 463 314 360 1013 245 1110
Fragments arranged by size:
1110 1013 796 463 360 314 245
NaeI GCC'GGC
Cuts at: 2482 2482
Size: 4301
NarI GG'CG_CC
Cuts at: 131 1732 1980 131
Size: 1601 248 2452
Fragments arranged by size:
2452 1601 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4301
NgoMIV G'CCGG_C
Cuts at: 2480 2480
Size: 4301
NspI r_CATG'y
Cuts at: 1354 1854 2761 1354
Size: 500 907 2894
Fragments arranged by size:
2894 907 500
PciI A'CATG_T
Cuts at: 2757 2757
Size: 4301
PflMI CCAn_nnn'nTGG
Cuts at: 473 473
Size: 4301
PmeI GTTT'AAAC
Cuts at: 1300 1300
Size: 4301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 4269
PpuMI rG'GwC_Cy
Cuts at: 213 1224 213
Size: 1011 3290
PstI C_TGCA'G
Cuts at: 897 1348 2033 897
Size: 451 685 3165
Fragments arranged by size:
3165 685 451
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4301
PvuII CAG'CTG
Cuts at: 1726 2086 1726
Size: 360 3941
RsrII CG'GwC_CG
Cuts at: 2496 2496
Size: 4301
SacI G_AGCT'C
Cuts at: 1082 1315 1082
Size: 233 4068
SalI G'TCGA_C
Cuts at: 1141 1338 1141
Size: 197 4104
SanDI GG'GwC_CC
Cuts at: 213 213
Size: 4301
SapI GCTCTTCn'nnn_
Cuts at: 1096 2324 2534 1096
Size: 1228 210 2863
Fragments arranged by size:
2863 1228 210
SbfI CC_TGCA'GG
Cuts at: 1348 1348
Size: 4301
ScaI AGT'ACT
Cuts at: 394 3818 394
Size: 3424 877
SfcI C'TryA_G
Cuts at: 33 645 748 893 933 1344 2029 3022 3213
Size: 612 103 145 40 411 685 993 191
Cuts at: 3213 33
Size: 1121
Fragments arranged by size:
1121 993 685 612 411 191 145 103
40
SfoI GGC'GCC
Cuts at: 132 1733 1981 132
Size: 1601 248 2452
Fragments arranged by size:
2452 1601 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
SmaI CCC'GGG
Cuts at: 191 1323 191
Size: 1132 3169
SmlI C'TyrA_G
Cuts at: 1456 2371 2863 3125 1456
Size: 915 492 262 2632
Fragments arranged by size:
2632 915 492 262
SnaBI TAC'GTA
Cuts at: 1293 1293
Size: 4301
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4301
SphI G_CATG'C
Cuts at: 1354 1854 1354
Size: 500 3801
StyI C'CwwG_G
Cuts at: 125 317 331 1435 1789 125
Size: 192 14 1104 354 2637
Fragments arranged by size:
2637 1104 354 192 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 480 484 1005 1029 1884 2017 2476 2685 480
Size: 4 521 24 855 133 459 209 2096
Fragments arranged by size:
2096 855 521 459 209 133 24 4
TatI w'GTAC_w
Cuts at: 392 755 1148 3816 392
Size: 363 393 2668 877
Fragments arranged by size:
2668 877 393 363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 4269
Tth111I GACn'n_nGTC
Cuts at: 2098 2098
Size: 4301
XbaI T'CTAG_A
Cuts at: 1332 1332
Size: 4301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1796 1796
Size: 4301
XmaI C'CCGG_G
Cuts at: 189 1321 189
Size: 1132 3169
XmnI GAAnn'nnTTC
Cuts at: 394 394
Size: 4301
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BsgI
BsiWI BsmBI BssHII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SrfI SspI StuI SwaI XhoI ZraI