Restriction map for
pF1KSDA0174
(Circular) (Six-base) MAPSORT of: pF1KSDA0174.seq Check: 7667 from: 1 to: 4229
pF1KSDA0174 4229 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 427 1267 427
Size: 840 3389
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4229
AclI AA'CG_TT
Cuts at: 4069 4069
Size: 4229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1181 2054 2486 3233 3501 1181
Size: 873 432 747 268 1909
Fragments arranged by size:
1909 873 747 432 268
AfeI AGC'GCT
Cuts at: 154 154
Size: 4229
AflII C'TTAA_G
Cuts at: 156 156
Size: 4229
AflIII A'CryG_T
Cuts at: 1581 2685 3662 1581
Size: 1104 977 2148
Fragments arranged by size:
2148 1104 977
AgeI A'CCGG_T
Cuts at: 2617 2617
Size: 4229
AlwNI CAG_nnn'CTG
Cuts at: 1159 1534 3101 1159
Size: 375 1567 2287
Fragments arranged by size:
2287 1567 375
ApaLI G'TGCA_C
Cuts at: 2999 2999
Size: 4229
ApoI r'AATT_y
Cuts at: 165 1233 3582 3838 165
Size: 1068 2349 256 556
Fragments arranged by size:
2349 1068 556 256
AseI AT'TA_AT
Cuts at: 20 983 20
Size: 963 3266
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
AvaI C'yCGr_G
Cuts at: 1249 1249
Size: 4229
BamHI G'GATC_C
Cuts at: 284 1254 284
Size: 970 3259
BanI G'GyrC_C
Cuts at: 807 1141 1245 1659 1907 1942 807
Size: 334 104 414 248 35 3094
Fragments arranged by size:
3094 414 334 248 104 35
BanII G_rGCy'C
Cuts at: 136 282 1243 2273 136
Size: 146 961 1030 2092
Fragments arranged by size:
2092 1030 961 146
BbeI G_GCGC'C
Cuts at: 1663 1911 1663
Size: 248 3981
BbsI GAAGACnn'nnnn_
Cuts at: 317 1169 317
Size: 852 3377
BbvCI CC'TCA_GC
Cuts at: 1152 1152
Size: 4229
BciVI GTATCCnnnnn_n'
Cuts at: 2127 2888 4229 2127
Size: 761 1341 2127
Fragments arranged by size:
2127 1341 761
BclI T'GATC_A
Cuts at: 489 1749 489
Size: 1260 2969
BglII A'GATC_T
Cuts at: 705 1744 3781 705
Size: 1039 2037 1153
Fragments arranged by size:
2037 1153 1039
BlpI GC'TnA_GC
Cuts at: 149 1341 149
Size: 1192 3037
Bme1580I G_kGCm'C
Cuts at: 1854 1947 3003 1854
Size: 93 1056 3080
Fragments arranged by size:
3080 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1441 1522 1857 4001 1441
Size: 81 335 2144 1669
Fragments arranged by size:
2144 1669 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 439 361
Size: 78 4151
Bpu10I CC'TnA_GC
Cuts at: 1152 1152
Size: 4229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 2284 2776 3074 1405
Size: 879 492 298 2560
Fragments arranged by size:
2560 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3933 3933
Size: 4229
BsaAI yAC'GTr
Cuts at: 1221 1582 1221
Size: 361 3868
BsaBI GATnn'nnATC
Cuts at: 6 1743 3780 6
Size: 1737 2037 455
Fragments arranged by size:
2037 1737 455
BsaHI Gr'CG_yC
Cuts at: 1660 1908 2610 4106 1660
Size: 248 702 1496 1783
Fragments arranged by size:
1783 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1418 1939 2617 2891 3038 3449 1418
Size: 521 678 274 147 411 2198
Fragments arranged by size:
2198 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 437 677 773 437
Size: 240 96 3893
Fragments arranged by size:
3893 240 96
BseYI C'CCAG_C
Cuts at: 129 262 1654 2412 2989 3485 129
Size: 133 1392 758 577 496 873
Fragments arranged by size:
1392 873 758 577 496 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 992 992
Size: 4229
BsiEI CG_ry'CG
Cuts at: 104 1771 1817 3025 104
Size: 1667 46 1208 1308
Fragments arranged by size:
1667 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 282 299 1243 2021 2211 3003 282
Size: 17 944 778 190 792 1508
Fragments arranged by size:
1508 944 792 778 190 17
Bsp1286I G_dGCh'C
Cuts at: 136 282 299 1243 1854 1947 2021 2211 2273
Size: 146 17 944 611 93 74 190 62
Cuts at: 2273 3003 136
Size: 730 1362
Fragments arranged by size:
1362 944 730 611 190 146 93 74
62 17
BspEI T'CCGG_A
Cuts at: 3449 3449
Size: 4229
BspHI T'CATG_A
Cuts at: 3405 3405
Size: 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 365 1074 1279 1493 1795 2176 365
Size: 709 205 214 302 381 2418
Fragments arranged by size:
2418 709 381 302 214 205
BsrBI CCG'CTC
Cuts at: 50 2521 2575 4059 4224 50
Size: 2471 54 1484 165 55
Fragments arranged by size:
2471 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2141 2141
Size: 4229
BsrFI r'CCGG_y
Cuts at: 2227 2408 2617 2227
Size: 181 209 3839
Fragments arranged by size:
3839 209 181
BssSI C'ACGA_G
Cuts at: 319 2500 2858 319
Size: 2181 358 1690
Fragments arranged by size:
2181 1690 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1606 1606
Size: 4229
BstBI TT'CG_AA
Cuts at: 110 2590 110
Size: 2480 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 606 606
Size: 4229
BstYI r'GATC_y
Cuts at: 1 284 335 705 1254 1721 1744 2079 2325
Size: 283 51 370 549 467 23 335 246
Cuts at: 2325 3337 3423 3435 3781 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 549 467 449 370 346 335 283
246 86 51 23 12
BtgI C'CryG_G
Cuts at: 329 329
Size: 4229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2179 2360 2523 2179
Size: 181 163 3885
Fragments arranged by size:
3885 181 163
BtsI GCAGTG_nn'
Cuts at: 862 1472 862
Size: 610 3619
DraI TTT'AAA
Cuts at: 144 1228 144
Size: 1084 3145
DrdI GACnn_nn'nnGTC
Cuts at: 670 1087 1935 2793 670
Size: 417 848 858 2106
Fragments arranged by size:
2106 858 848 417
EaeI y'GGCC_r
Cuts at: 1814 1988 2379 2406 3751 1814
Size: 174 391 27 1345 2292
Fragments arranged by size:
2292 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1814 1814
Size: 4229
EarI CTCTTCn'nnn_
Cuts at: 750 1008 1193 2252 2462 750
Size: 258 185 1059 210 2517
Fragments arranged by size:
2517 1059 258 210 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2437 2747 2893 4039 2437
Size: 310 146 1146 2627
Fragments arranged by size:
2627 1146 310 146
EcoICRI GAG'CTC
Cuts at: 280 1241 280
Size: 961 3268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 439 1181 2054 2486 3233 3501 361
Size: 78 742 873 432 747 268 1089
Fragments arranged by size:
1089 873 747 742 432 268 78
EcoNI CCTnn'n_nnAGG
Cuts at: 322 755 322
Size: 433 3796
EcoO109I rG'GnC_Cy
Cuts at: 748 1368 748
Size: 620 3609
EcoRI G'AATT_C
Cuts at: 1233 1233
Size: 4229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 4229
FspI TGC'GCA
Cuts at: 2010 2010
Size: 4229
HaeII r_GCGC'y
Cuts at: 156 1663 1911 2933 3912 156
Size: 1507 248 1022 979 473
Fragments arranged by size:
1507 1022 979 473 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 269 301 1005 1037 1090 1122 2071 2101 2103
Size: 32 704 32 53 32 949 30 2
Cuts at: 2103 2133 269
Size: 30 2365
Fragments arranged by size:
2365 949 704 53 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1268 1268
Size: 4229
HindIII A'AGCT_T
Cuts at: 1051 1051
Size: 4229
KasI G'GCGC_C
Cuts at: 1659 1907 1659
Size: 248 3981
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4229
MluI A'CGCG_T
Cuts at: 3662 3662
Size: 4229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 699 2900 3084 699
Size: 2201 184 1844
Fragments arranged by size:
2201 1844 184
MscI TGG'CCA
Cuts at: 1990 1990
Size: 4229
MslI CAynn'nnrTG
Cuts at: 823 829 2218 2345 823
Size: 6 1389 127 2707
Fragments arranged by size:
2707 1389 127 6
MspA1I CmG'CkG
Cuts at: 1340 1654 2014 3027 3272 1340
Size: 314 360 1013 245 2297
Fragments arranged by size:
2297 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2410 2410
Size: 4229
NarI GG'CG_CC
Cuts at: 1660 1908 1660
Size: 248 3981
NcoI C'CATG_G
Cuts at: 329 329
Size: 4229
NgoMIV G'CCGG_C
Cuts at: 2408 2408
Size: 4229
NspI r_CATG'y
Cuts at: 1282 1782 2689 1282
Size: 500 907 2822
Fragments arranged by size:
2822 907 500
PciI A'CATG_T
Cuts at: 2685 2685
Size: 4229
PflMI CCAn_nnn'nTGG
Cuts at: 790 790
Size: 4229
PfoI T'CCnGG_A
Cuts at: 305 338 1023 1186 305
Size: 33 685 163 3348
Fragments arranged by size:
3348 685 163 33
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 4197
PshAI GACnn'nnGTC
Cuts at: 456 456
Size: 4229
PstI C_TGCA'G
Cuts at: 1071 1276 1961 1071
Size: 205 685 3339
Fragments arranged by size:
3339 685 205
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4229
PvuII CAG'CTG
Cuts at: 1654 2014 1654
Size: 360 3869
RsrII CG'GwC_CG
Cuts at: 2424 2424
Size: 4229
SacI G_AGCT'C
Cuts at: 282 1243 282
Size: 961 3268
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4229
SapI GCTCTTCn'nnn_
Cuts at: 1193 2252 2462 1193
Size: 1059 210 2960
Fragments arranged by size:
2960 1059 210
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4229
ScaI AGT'ACT
Cuts at: 3746 3746
Size: 4229
SfcI C'TryA_G
Cuts at: 33 971 1067 1272 1957 2950 3141 33
Size: 938 96 205 685 993 191 1121
Fragments arranged by size:
1121 993 938 685 205 191 96
SfoI GGC'GCC
Cuts at: 1661 1909 1661
Size: 248 3981
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
SmaI CCC'GGG
Cuts at: 1251 1251
Size: 4229
SmlI C'TyrA_G
Cuts at: 156 1384 2299 2791 3053 156
Size: 1228 915 492 262 1332
Fragments arranged by size:
1332 1228 915 492 262
SnaBI TAC'GTA
Cuts at: 1221 1221
Size: 4229
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4229
SphI G_CATG'C
Cuts at: 1282 1782 1282
Size: 500 3729
SspI AAT'ATT
Cuts at: 927 927
Size: 4229
StuI AGG'CCT
Cuts at: 916 916
Size: 4229
StyI C'CwwG_G
Cuts at: 329 1363 1717 329
Size: 1034 354 2841
Fragments arranged by size:
2841 1034 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 612 1812 1945 2404 2613 612
Size: 1200 133 459 209 2228
Fragments arranged by size:
2228 1200 459 209 133
TatI w'GTAC_w
Cuts at: 347 3744 347
Size: 3397 832
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 587 619 3746 3778 587
Size: 32 3127 32 1038
Fragments arranged by size:
3127 1038 32 32
Tth111I GACn'n_nGTC
Cuts at: 2026 2026
Size: 4229
XbaI T'CTAG_A
Cuts at: 1260 1260
Size: 4229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 336 606 1724 336
Size: 270 1118 2841
Fragments arranged by size:
2841 1118 270
XmaI C'CCGG_G
Cuts at: 1249 1249
Size: 4229
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PshAI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SwaI XhoI XmnI ZraI