Restriction map for
pF1KSDA0173
(Circular) (Six-base) MAPSORT of: pF1KSDA0173.seq Check: 7274 from: 1 to: 6734
pF1KSDA0173 6734 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 988 3772 988
Size: 2784 3950
Acc65I G'GTAC_C
Cuts at: 3750 3750
Size: 6734
AclI AA'CG_TT
Cuts at: 1871 6574 1871
Size: 4703 2031
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 862 1670 2653 3426 3527 4559 4991 5738
Size: 176 808 983 773 101 1032 432 747
Cuts at: 5738 6006 686
Size: 268 1414
Fragments arranged by size:
1414 1032 983 808 773 747 432 268
176 101
AflIII A'CryG_T
Cuts at: 4086 5190 6167 4086
Size: 1104 977 4653
Fragments arranged by size:
4653 1104 977
AgeI A'CCGG_T
Cuts at: 5122 5122
Size: 6734
AleI CACnn'nnGTG
Cuts at: 811 811
Size: 6734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 829 3065 829
Size: 2236 4498
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1114 1146 1114
Size: 32 6702
AlwNI CAG_nnn'CTG
Cuts at: 211 248 431 516 688 840 1621 2978 4039
Size: 37 183 85 172 152 781 1357 1061
Cuts at: 4039 5606 211
Size: 1567 1339
Fragments arranged by size:
1567 1357 1339 1061 781 183 172 152
85 37
ApaI G_GGCC'C
Cuts at: 331 331
Size: 6734
ApaLI G'TGCA_C
Cuts at: 908 5504 908
Size: 4596 2138
ApoI r'AATT_y
Cuts at: 1799 3089 3200 3738 6087 6343 1799
Size: 1290 111 538 2349 256 2190
Fragments arranged by size:
2349 2190 1290 538 256 111
AseI AT'TA_AT
Cuts at: 20 20
Size: 6734
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6734
AvaI C'yCGr_G
Cuts at: 1898 2303 3754 1898
Size: 405 1451 4878
Fragments arranged by size:
4878 1451 405
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2359 2392 2359
Size: 33 6701
BamHI G'GATC_C
Cuts at: 1110 3759 1110
Size: 2649 4085
BanI G'GyrC_C
Cuts at: 933 1330 3750 4164 4412 4447 933
Size: 397 2420 414 248 35 3220
Fragments arranged by size:
3220 2420 414 397 248 35
BanII G_rGCy'C
Cuts at: 331 544 1418 2735 3611 3721 3748 4778 331
Size: 213 874 1317 876 110 27 1030 2287
Fragments arranged by size:
2287 1317 1030 876 874 213 110 27
BbeI G_GCGC'C
Cuts at: 4168 4416 4168
Size: 248 6486
BbsI GAAGACnn'nnnn_
Cuts at: 618 1356 1691 3515 3541 618
Size: 738 335 1824 26 3811
Fragments arranged by size:
3811 1824 738 335 26
BbvCI CC'TCA_GC
Cuts at: 132 1455 132
Size: 1323 5411
BciVI GTATCCnnnnn_n'
Cuts at: 4632 5393 6734 4632
Size: 761 1341 4632
Fragments arranged by size:
4632 1341 761
BclI T'GATC_A
Cuts at: 3096 3638 4254 3096
Size: 542 616 5576
Fragments arranged by size:
5576 616 542
BfrBI ATG'CAT
Cuts at: 3112 3112
Size: 6734
BglI GCCn_nnn'nGGC
Cuts at: 341 607 1063 1519 2180 341
Size: 266 456 456 661 4895
Fragments arranged by size:
4895 661 456 456 266
BglII A'GATC_T
Cuts at: 3318 4249 6286 3318
Size: 931 2037 3766
Fragments arranged by size:
3766 2037 931
BlpI GC'TnA_GC
Cuts at: 3846 3846
Size: 6734
Bme1580I G_kGCm'C
Cuts at: 331 912 938 975 2631 4359 4452 5508 331
Size: 581 26 37 1656 1728 93 1056 1557
Fragments arranged by size:
1728 1656 1557 1056 581 93 37 26
BmgBI CAC'GTC
Cuts at: 3426 3426
Size: 6734
BmrI ACTGGGnnnn_n'
Cuts at: 2202 3566 3946 4027 4362 6506 2202
Size: 1364 380 81 335 2144 2430
Fragments arranged by size:
2430 2144 1364 380 335 81
BmtI G_CTAG'C
Cuts at: 998 998
Size: 6734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1608 1743 1755 5101 1608
Size: 135 12 3346 3241
Fragments arranged by size:
3346 3241 135 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 471 538 920 1463 1612 2853 3020 3362 471
Size: 67 382 543 149 1241 167 342 3843
Fragments arranged by size:
3843 1241 543 382 342 167 149 67
Bpu10I CC'TnA_GC
Cuts at: 132 1455 2653 132
Size: 1323 1198 4213
Fragments arranged by size:
4213 1323 1198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2737 2809 3524 3910 4789 5281 5579 2737
Size: 72 715 386 879 492 298 3892
Fragments arranged by size:
3892 879 715 492 386 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 1560 3392 6438 1560
Size: 1832 3046 1856
Fragments arranged by size:
3046 1856 1832
BsaAI yAC'GTr
Cuts at: 2164 3726 4087 2164
Size: 1562 361 4811
Fragments arranged by size:
4811 1562 361
BsaBI GATnn'nnATC
Cuts at: 6 4248 6285 6
Size: 4242 2037 455
Fragments arranged by size:
4242 2037 455
BsaHI Gr'CG_yC
Cuts at: 344 2231 2678 4165 4413 5115 6611 344
Size: 1887 447 1487 248 702 1496 467
Fragments arranged by size:
1887 1496 1487 702 467 447 248
BsaWI w'CCGG_w
Cuts at: 1043 3326 3923 4444 5122 5396 5543 5954 1043
Size: 2283 597 521 678 274 147 411 1823
Fragments arranged by size:
2283 1823 678 597 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 298 328 431 461 469 499 1114 1144 298
Size: 30 103 30 8 30 615 30 5888
Fragments arranged by size:
5888 615 103 30 30 30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 642 1443 1588 1728 1729 1732 3210 642
Size: 801 145 140 1 3 1478 4166
Fragments arranged by size:
4166 1478 801 145 140 3 1
BseYI C'CCAG_C
Cuts at: 353 376 638 688 957 1222 1545 1759 2037
Size: 23 262 50 269 265 323 214 278
Cuts at: 2037 2045 2971 3465 3610 4159 4917 5494 5990
Size: 8 926 494 145 549 758 577 496
Cuts at: 5990 353
Size: 1097
Fragments arranged by size:
1097 926 758 577 549 496 494 323
278 269 265 262 214 145 50 23
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3035 3035
Size: 6734
BsiEI CG_ry'CG
Cuts at: 104 4276 4322 5530 104
Size: 4172 46 1208 1308
Fragments arranged by size:
4172 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 912 1086 1738 1963 3649 3748 4526 4716 5508
Size: 174 652 225 1686 99 778 190 792
Cuts at: 5508 912
Size: 2138
Fragments arranged by size:
2138 1686 792 778 652 225 190 174
99
BsmI GAATG_Cn'
Cuts at: 1385 2580 1385
Size: 1195 5539
Bsp1286I G_dGCh'C
Cuts at: 331 544 912 938 975 1086 1418 1738 1963
Size: 213 368 26 37 111 332 320 225
Cuts at: 1963 2631 2735 3611 3649 3721 3748 4359 4452
Size: 668 104 876 38 72 27 611 93
Cuts at: 4452 4526 4716 4778 5508 331
Size: 74 190 62 730 1557
Fragments arranged by size:
1557 876 730 668 611 368 332 320
225 213 190 111 104 93 74 72
62 38 37 27 26
BspEI T'CCGG_A
Cuts at: 3326 5954 3326
Size: 2628 4106
BspHI T'CATG_A
Cuts at: 1824 5910 1824
Size: 4086 2648
BspMI ACCTGCnnnn'nnnn_
Cuts at: 219 2426 2920 3784 3998 4300 4681 219
Size: 2207 494 864 214 302 381 2272
Fragments arranged by size:
2272 2207 864 494 381 302 214
BsrBI CCG'CTC
Cuts at: 50 485 5026 5080 6564 6729 50
Size: 435 4541 54 1484 165 55
Fragments arranged by size:
4541 1484 435 165 55 54
BsrDI GCAATG_nn'
Cuts at: 410 4646 410
Size: 4236 2498
BsrFI r'CCGG_y
Cuts at: 2141 4732 4913 5122 2141
Size: 2591 181 209 3753
Fragments arranged by size:
3753 2591 209 181
BssSI C'ACGA_G
Cuts at: 5005 5363 5005
Size: 358 6376
BstAPI GCAn_nnn'nTGC
Cuts at: 4111 4111
Size: 6734
BstBI TT'CG_AA
Cuts at: 110 5095 110
Size: 4985 1749
BstEII G'GTnAC_C
Cuts at: 2050 2050
Size: 6734
BstXI CCAn_nnnn'nTGG
Cuts at: 1545 1587 1545
Size: 42 6692
BstYI r'GATC_y
Cuts at: 1 737 863 1110 2423 3318 3759 4226 4249
Size: 736 126 247 1313 895 441 467 23
Cuts at: 4249 4584 4830 5842 5928 5940 6286 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1313 1012 895 736 467 449 441 346
335 247 246 126 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 198 254 1292 1571 198
Size: 56 1038 279 5361
Fragments arranged by size:
5361 1038 279 56
BtgI C'CryG_G
Cuts at: 125 401 620 632 644 3183 3294 125
Size: 276 219 12 12 2539 111 3565
Fragments arranged by size:
3565 2539 276 219 111 12 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4684 4865 5028 4684
Size: 181 163 6390
Fragments arranged by size:
6390 181 163
BtsI GCAGTG_nn'
Cuts at: 596 3047 3977 596
Size: 2451 930 3353
Fragments arranged by size:
3353 2451 930
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 171 206 1005 1040 171
Size: 35 799 35 5865
Fragments arranged by size:
5865 799 35 35
DraI TTT'AAA
Cuts at: 3733 3733
Size: 6734
DrdI GACnn_nn'nnGTC
Cuts at: 1360 4440 5298 1360
Size: 3080 858 2796
Fragments arranged by size:
3080 2796 858
EaeI y'GGCC_r
Cuts at: 796 1520 1772 4319 4493 4884 4911 6256 796
Size: 724 252 2547 174 391 27 1345 1274
Fragments arranged by size:
2547 1345 1274 724 391 252 174 27
EagI C'GGCC_G
Cuts at: 4319 4319
Size: 6734
EarI CTCTTCn'nnn_
Cuts at: 703 759 1035 1539 1706 2515 4757 4967 703
Size: 56 276 504 167 809 2242 210 2470
Fragments arranged by size:
2470 2242 809 504 276 210 167 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 154 2166 4942 5252 5398 6544 154
Size: 2012 2776 310 146 1146 344
Fragments arranged by size:
2776 2012 1146 344 310 146
EcoICRI GAG'CTC
Cuts at: 3746 3746
Size: 6734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 471 538 686 862 920 1463 1612 1670 2653
Size: 67 148 176 58 543 149 58 983
Cuts at: 2653 2853 3020 3362 3426 3527 4559 4991 5738
Size: 200 167 342 64 101 1032 432 747
Cuts at: 5738 6006 471
Size: 268 1199
Fragments arranged by size:
1199 1032 983 747 543 432 342 268
200 176 167 149 148 101 67 64
58 58
EcoNI CCTnn'n_nnAGG
Cuts at: 2224 2224
Size: 6734
EcoO109I rG'GnC_Cy
Cuts at: 837 1320 1483 2357 2835 3873 837
Size: 483 163 874 478 1038 3698
Fragments arranged by size:
3698 1038 874 483 478 163
EcoRI G'AATT_C
Cuts at: 3738 3738
Size: 6734
EcoRV GAT'ATC
Cuts at: 2886 2958 2886
Size: 72 6662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3565 4753 3565
Size: 1188 5546
FspI TGC'GCA
Cuts at: 2245 4515 2245
Size: 2270 4464
HaeII r_GCGC'y
Cuts at: 4168 4416 5438 6417 4168
Size: 248 1022 979 4485
Fragments arranged by size:
4485 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 729 761 1081 1113 1291 1323 1832
Size: 32 399 32 320 32 178 32 509
Cuts at: 1832 1864 4576 4606 4608 4638 298
Size: 32 2712 30 2 30 2394
Fragments arranged by size:
2712 2394 509 399 320 178 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 924 2847 3773 924
Size: 1923 926 3885
Fragments arranged by size:
3885 1923 926
HindIII A'AGCT_T
Cuts at: 3304 3304
Size: 6734
KasI G'GCGC_C
Cuts at: 4164 4412 4164
Size: 248 6486
KpnI G_GTAC'C
Cuts at: 3754 3754
Size: 6734
MluI A'CGCG_T
Cuts at: 6167 6167
Size: 6734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1138 1331 1863 5405 5589 1138
Size: 193 532 3542 184 2283
Fragments arranged by size:
3542 2283 532 193 184
MscI TGG'CCA
Cuts at: 798 1522 1774 4495 798
Size: 724 252 2721 3037
Fragments arranged by size:
3037 2721 724 252
MslI CAynn'nnrTG
Cuts at: 811 1409 4723 4850 811
Size: 598 3314 127 2695
Fragments arranged by size:
3314 2695 598 127
MspA1I CmG'CkG
Cuts at: 685 961 1545 2402 2978 3845 4159 4519 5532
Size: 276 584 857 576 867 314 360 1013
Cuts at: 5532 5777 685
Size: 245 1642
Fragments arranged by size:
1642 1013 867 857 584 576 360 314
276 245
NaeI GCC'GGC
Cuts at: 4915 4915
Size: 6734
NarI GG'CG_CC
Cuts at: 4165 4413 4165
Size: 248 6486
NcoI C'CATG_G
Cuts at: 125 620 632 644 3294 125
Size: 495 12 12 2650 3565
Fragments arranged by size:
3565 2650 495 12 12
NgoMIV G'CCGG_C
Cuts at: 4913 4913
Size: 6734
NheI G'CTAG_C
Cuts at: 994 994
Size: 6734
NsiI A_TGCA'T
Cuts at: 3114 3114
Size: 6734
NspI r_CATG'y
Cuts at: 1408 3046 3787 4287 5194 1408
Size: 1638 741 500 907 2948
Fragments arranged by size:
2948 1638 907 741 500
PasI CC'CwG_GG
Cuts at: 1925 2831 3013 1925
Size: 906 182 5646
Fragments arranged by size:
5646 906 182
PciI A'CATG_T
Cuts at: 5190 5190
Size: 6734
PflMI CCAn_nnn'nTGG
Cuts at: 516 645 3278 516
Size: 129 2633 3972
Fragments arranged by size:
3972 2633 129
PfoI T'CCnGG_A
Cuts at: 449 2837 449
Size: 2388 4346
PmeI GTTT'AAAC
Cuts at: 3733 3733
Size: 6734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 656 688 1114 1146 5873 5905 656
Size: 32 426 32 4727 32 1485
Fragments arranged by size:
4727 1485 426 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1320 1483 2835 1320
Size: 163 1352 5219
Fragments arranged by size:
5219 1352 163
PshAI GACnn'nnGTC
Cuts at: 3023 3023
Size: 6734
PspOMI G'GGCC_C
Cuts at: 327 327
Size: 6734
PspXI vC'TCGA_Gb
Cuts at: 2303 2303
Size: 6734
PstI C_TGCA'G
Cuts at: 2983 3781 4466 2983
Size: 798 685 5251
Fragments arranged by size:
5251 798 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6734
PvuII CAG'CTG
Cuts at: 961 1545 2978 4159 4519 961
Size: 584 1433 1181 360 3176
Fragments arranged by size:
3176 1433 1181 584 360
RsrII CG'GwC_CG
Cuts at: 4929 4929
Size: 6734
SacI G_AGCT'C
Cuts at: 3748 3748
Size: 6734
SalI G'TCGA_C
Cuts at: 3771 3771
Size: 6734
SapI GCTCTTCn'nnn_
Cuts at: 703 2515 4757 4967 703
Size: 1812 2242 210 2470
Fragments arranged by size:
2470 2242 1812 210
SbfI CC_TGCA'GG
Cuts at: 3781 3781
Size: 6734
ScaI AGT'ACT
Cuts at: 6251 6251
Size: 6734
SfcI C'TryA_G
Cuts at: 33 150 492 1854 2556 2775 2979 3777 4462
Size: 117 342 1362 702 219 204 798 685
Cuts at: 4462 5455 5646 33
Size: 993 191 1121
Fragments arranged by size:
1362 1121 993 798 702 685 342 219
204 191 117
SfoI GGC'GCC
Cuts at: 4166 4414 4166
Size: 248 6486
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6734
SmaI CCC'GGG
Cuts at: 3756 3756
Size: 6734
SmlI C'TyrA_G
Cuts at: 2303 2752 2824 3539 3889 4804 5296 5558 2303
Size: 449 72 715 350 915 492 262 3479
Fragments arranged by size:
3479 915 715 492 449 350 262 72
SnaBI TAC'GTA
Cuts at: 3726 3726
Size: 6734
SpeI A'CTAG_T
Cuts at: 67 1668 67
Size: 1601 5133
SphI G_CATG'C
Cuts at: 3787 4287 3787
Size: 500 6234
StuI AGG'CCT
Cuts at: 335 335
Size: 6734
StyI C'CwwG_G
Cuts at: 125 620 632 644 1343 2526 3294 3868 4222
Size: 495 12 12 699 1183 768 574 354
Cuts at: 4222 125
Size: 2637
Fragments arranged by size:
2637 1183 768 699 574 495 354 12
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 873 1299 3289 4317 4450 4909 5118 873
Size: 426 1990 1028 133 459 209 2489
Fragments arranged by size:
2489 1990 1028 459 426 209 133
TatI w'GTAC_w
Cuts at: 6249 6249
Size: 6734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 412 444 848 880 994 1026 6251 6283 412
Size: 32 404 32 114 32 5225 32 863
Fragments arranged by size:
5225 863 404 114 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 312 4531 312
Size: 4219 2515
XbaI T'CTAG_A
Cuts at: 1783 3765 1783
Size: 1982 4752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 618 1767 2791 2877 4229 618
Size: 1149 1024 86 1352 3123
Fragments arranged by size:
3123 1352 1149 1024 86
XhoI C'TCGA_G
Cuts at: 2303 2303
Size: 6734
XmaI C'CCGG_G
Cuts at: 3754 3754
Size: 6734
XmnI GAAnn'nnTTC
Cuts at: 2191 2191
Size: 6734
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AscI AvrII BcgI
BsiWI BsmBI BsrGI BssHII BstZ17I ClaI DraIII FseI
FspAI HpaI MfeI NdeI NotI NruI PacI PmlI
PsiI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI SwaI ZraI