Restriction map for
pF1KSDA0172
(Circular) (Six-base) MAPSORT of: pF1KSDA0172.seq Check: 7878 from: 1 to: 7193
pF1KSDA0172 7193 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 160 911 954 160
Size: 751 43 6399
Fragments arranged by size:
6399 751 43
AatII G_ACGT'C
Cuts at: 4150 4150
Size: 7193
AccI GT'mk_AC
Cuts at: 4231 4231
Size: 7193
Acc65I G'GTAC_C
Cuts at: 4209 4209
Size: 7193
AclI AA'CG_TT
Cuts at: 2344 7033 2344
Size: 4689 2504
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 967 1493 2792 2845 3067 3091 5018 5450
Size: 222 526 1299 53 222 24 1927 432
Cuts at: 5450 6197 6465 745
Size: 747 268 1473
Fragments arranged by size:
1927 1473 1299 747 526 432 268 222
222 53 24
AflII C'TTAA_G
Cuts at: 2877 2877
Size: 7193
AflIII A'CryG_T
Cuts at: 1730 1732 4545 5649 6626 1730
Size: 2 2813 1104 977 2297
Fragments arranged by size:
2813 2297 1104 977 2
AgeI A'CCGG_T
Cuts at: 5581 5581
Size: 7193
AleI CACnn'nnGTG
Cuts at: 877 877
Size: 7193
AlwNI CAG_nnn'CTG
Cuts at: 1280 1388 1922 2278 2622 2643 4498 6065 1280
Size: 108 534 356 344 21 1855 1567 2408
Fragments arranged by size:
2408 1855 1567 534 356 344 108 21
ApaI G_GGCC'C
Cuts at: 1140 1140
Size: 7193
ApaLI G'TGCA_C
Cuts at: 2164 5963 2164
Size: 3799 3394
ApoI r'AATT_y
Cuts at: 4197 6546 6802 4197
Size: 2349 256 4588
Fragments arranged by size:
4588 2349 256
AseI AT'TA_AT
Cuts at: 20 3122 20
Size: 3102 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7193
AvaI C'yCGr_G
Cuts at: 1133 2066 3254 4213 1133
Size: 933 1188 959 4113
Fragments arranged by size:
4113 1188 959 933
AvrII C'CTAG_G
Cuts at: 4129 4129
Size: 7193
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 762 795 762
Size: 33 7160
BamHI G'GATC_C
Cuts at: 4218 4218
Size: 7193
BanI G'GyrC_C
Cuts at: 697 1336 3272 4123 4209 4623 4871 4906 697
Size: 639 1936 851 86 414 248 35 2984
Fragments arranged by size:
2984 1936 851 639 414 248 86 35
BanII G_rGCy'C
Cuts at: 838 862 895 1140 1301 1496 2707 3928 4207
Size: 24 33 245 161 195 1211 1221 279
Cuts at: 4207 5237 838
Size: 1030 2794
Fragments arranged by size:
2794 1221 1211 1030 279 245 195 161
33 24
BbeI G_GCGC'C
Cuts at: 4627 4875 4627
Size: 248 6945
BbsI GAAGACnn'nnnn_
Cuts at: 621 2395 3254 4150 621
Size: 1774 859 896 3664
Fragments arranged by size:
3664 1774 896 859
BbvCI CC'TCA_GC
Cuts at: 2779 2779
Size: 7193
BciVI GTATCCnnnnn_n'
Cuts at: 763 2623 3224 5091 5852 7193 763
Size: 1860 601 1867 761 1341 763
Fragments arranged by size:
1867 1860 1341 763 761 601
BclI T'GATC_A
Cuts at: 4713 4713
Size: 7193
BfrBI ATG'CAT
Cuts at: 1857 3022 1857
Size: 1165 6028
BglI GCCn_nnn'nGGC
Cuts at: 1642 2113 3895 1642
Size: 471 1782 4940
Fragments arranged by size:
4940 1782 471
BglII A'GATC_T
Cuts at: 995 1529 4708 6745 995
Size: 534 3179 2037 1443
Fragments arranged by size:
3179 2037 1443 534
BlpI GC'TnA_GC
Cuts at: 4305 4305
Size: 7193
Bme1580I G_kGCm'C
Cuts at: 436 700 1140 2168 3275 3962 4126 4818 4911
Size: 264 440 1028 1107 687 164 692 93
Cuts at: 4911 5967 436
Size: 1056 1662
Fragments arranged by size:
1662 1107 1056 1028 692 687 440 264
164 93
BmgBI CAC'GTC
Cuts at: 2034 2034
Size: 7193
BmrI ACTGGGnnnn_n'
Cuts at: 871 4107 4405 4486 4821 6965 871
Size: 3236 298 81 335 2144 1099
Fragments arranged by size:
3236 2144 1099 335 298 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1308 2079 5560 1308
Size: 771 3481 2941
Fragments arranged by size:
3481 2941 771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 970 976 1276 1365 2569 2963 3463 520
Size: 450 6 300 89 1204 394 500 4250
Fragments arranged by size:
4250 1204 500 450 394 300 89 6
Bpu10I CC'TnA_GC
Cuts at: 856 861 1300 1811 2779 2900 856
Size: 5 439 511 968 121 5149
Fragments arranged by size:
5149 968 511 439 121 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1957 2549 4369 5248 5740 6038 1957
Size: 592 1820 879 492 298 3112
Fragments arranged by size:
3112 1820 879 592 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 182 220 496 598 1035 1183 1688 2449 6897
Size: 38 276 102 437 148 505 761 4448
Cuts at: 6897 182
Size: 478
Fragments arranged by size:
4448 761 505 478 437 276 148 102
38
BsaAI yAC'GTr
Cuts at: 1733 3963 4185 4546 1733
Size: 2230 222 361 4380
Fragments arranged by size:
4380 2230 361 222
BsaBI GATnn'nnATC
Cuts at: 6 4707 6744 6
Size: 4701 2037 455
Fragments arranged by size:
4701 2037 455
BsaHI Gr'CG_yC
Cuts at: 4147 4624 4872 5574 7070 4147
Size: 477 248 702 1496 4270
Fragments arranged by size:
4270 1496 702 477 248
BsaWI w'CCGG_w
Cuts at: 4382 4903 5581 5855 6002 6413 4382
Size: 521 678 274 147 411 5162
Fragments arranged by size:
5162 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 456 486 548 578 1363 1393 2293 2323 2961
Size: 30 62 30 785 30 900 30 638
Cuts at: 2961 2991 3910 3940 456
Size: 30 919 30 3709
Fragments arranged by size:
3709 919 900 785 638 62 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 973 1189 1281 1357 1960 2301 3244 3247 3250
Size: 216 92 76 603 341 943 3 3
Cuts at: 3250 3253 973
Size: 3 4913
Fragments arranged by size:
4913 943 603 341 216 92 76 3
3 3
BseYI C'CCAG_C
Cuts at: 804 1115 1646 1870 2558 2894 3989 4618 5376
Size: 311 531 224 688 336 1095 629 758
Cuts at: 5376 5953 6449 804
Size: 577 496 1548
Fragments arranged by size:
1548 1095 758 688 629 577 531 496
336 311 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1705 2193 1705
Size: 488 6705
BsiEI CG_ry'CG
Cuts at: 104 4735 4781 5989 104
Size: 4631 46 1208 1308
Fragments arranged by size:
4631 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 838 862 986 1207 1301 1496 2168 3956 4207
Size: 24 124 221 94 195 672 1788 251
Cuts at: 4207 4985 5175 5967 838
Size: 778 190 792 2064
Fragments arranged by size:
2064 1788 792 778 672 251 221 195
190 124 94 24
BsmI GAATG_Cn'
Cuts at: 3810 3810
Size: 7193
BsmBI CGTCTCn'nnnn_
Cuts at: 2179 2257 4154 2179
Size: 78 1897 5218
Fragments arranged by size:
5218 1897 78
Bsp1286I G_dGCh'C
Cuts at: 436 700 838 862 895 986 1140 1207 1301
Size: 264 138 24 33 91 154 67 94
Cuts at: 1301 1496 2168 2707 3275 3928 3956 3962 4126
Size: 195 672 539 568 653 28 6 164
Cuts at: 4126 4207 4818 4911 4985 5175 5237 5967 436
Size: 81 611 93 74 190 62 730 1662
Fragments arranged by size:
1662 730 672 653 611 568 539 264
195 190 164 154 138 94 93 91
81 74 67 62 33 28 24 6
BspEI T'CCGG_A
Cuts at: 6413 6413
Size: 7193
BspHI T'CATG_A
Cuts at: 2753 3056 6369 2753
Size: 303 3313 3577
Fragments arranged by size:
3577 3313 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 160 911 954 3318 4243 4457 4759 5140 160
Size: 751 43 2364 925 214 302 381 2213
Fragments arranged by size:
2364 2213 925 751 381 302 214 43
BsrBI CCG'CTC
Cuts at: 50 5485 5539 7023 7188 50
Size: 5435 54 1484 165 55
Fragments arranged by size:
5435 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 929 5105 929
Size: 4176 3017
BsrFI r'CCGG_y
Cuts at: 1319 5191 5372 5581 1319
Size: 3872 181 209 2931
Fragments arranged by size:
3872 2931 209 181
BsrGI T'GTAC_A
Cuts at: 3025 3025
Size: 7193
BssSI C'ACGA_G
Cuts at: 3484 5464 5822 3484
Size: 1980 358 4855
Fragments arranged by size:
4855 1980 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1775 2643 4570 1775
Size: 868 1927 4398
Fragments arranged by size:
4398 1927 868
BstBI TT'CG_AA
Cuts at: 110 5554 110
Size: 5444 1749
BstEII G'GTnAC_C
Cuts at: 4006 4006
Size: 7193
BstXI CCAn_nnnn'nTGG
Cuts at: 781 3729 781
Size: 2948 4245
BstYI r'GATC_y
Cuts at: 1 866 995 1265 1529 4218 4685 4708 5043
Size: 865 129 270 264 2689 467 23 335
Cuts at: 5043 5289 6301 6387 6399 6745 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2689 1012 865 467 449 346 335 270
264 246 129 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 159 159
Size: 7193
BtgI C'CryG_G
Cuts at: 125 827 1748 3527 3929 125
Size: 702 921 1779 402 3389
Fragments arranged by size:
3389 1779 921 702 402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4056 5143 5324 5487 4056
Size: 1087 181 163 5762
Fragments arranged by size:
5762 1087 181 163
BtsI GCAGTG_nn'
Cuts at: 159 436 1685 2123 2641 4031 4436 159
Size: 277 1249 438 518 1390 405 2916
Fragments arranged by size:
2916 1390 1249 518 438 405 277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1056 1091 2462 2497 1056
Size: 35 1371 35 5752
Fragments arranged by size:
5752 1371 35 35
DraI TTT'AAA
Cuts at: 4192 4192
Size: 7193
DraIII CAC_nnn'GTG
Cuts at: 655 655
Size: 7193
DrdI GACnn_nn'nnGTC
Cuts at: 1702 2038 2397 4899 5757 1702
Size: 336 359 2502 858 3138
Fragments arranged by size:
3138 2502 858 359 336
EaeI y'GGCC_r
Cuts at: 677 926 2189 2355 4778 4952 5343 5370 6715
Size: 249 1263 166 2423 174 391 27 1345
Cuts at: 6715 677
Size: 1155
Fragments arranged by size:
2423 1345 1263 1155 391 249 174 166
27
EagI C'GGCC_G
Cuts at: 4778 4778
Size: 7193
EarI CTCTTCn'nnn_
Cuts at: 313 1007 2768 3180 3221 5216 5426 313
Size: 694 1761 412 41 1995 210 2080
Fragments arranged by size:
2080 1995 1761 694 412 210 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 339 832 5401 5711 5857 7003 339
Size: 493 4569 310 146 1146 529
Fragments arranged by size:
4569 1146 529 493 310 146
EcoICRI GAG'CTC
Cuts at: 836 860 1299 1494 4205 836
Size: 24 439 195 2711 3824
Fragments arranged by size:
3824 2711 439 195 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 745 967 970 976 1276 1365 1493 2569
Size: 225 222 3 6 300 89 128 1076
Cuts at: 2569 2792 2845 2963 3067 3091 3463 5018 5450
Size: 223 53 118 104 24 372 1555 432
Cuts at: 5450 6197 6465 520
Size: 747 268 1248
Fragments arranged by size:
1555 1248 1076 747 432 372 300 268
225 223 222 128 118 104 89 53
24 6 3
EcoO109I rG'GnC_Cy
Cuts at: 1385 1742 1807 2856 2915 3879 4332 1385
Size: 357 65 1049 59 964 453 4246
Fragments arranged by size:
4246 1049 964 453 357 65 59
EcoRI G'AATT_C
Cuts at: 4197 4197
Size: 7193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1567 1998 2590 3194 5212 1567
Size: 431 592 604 2018 3548
Fragments arranged by size:
3548 2018 604 592 431
FspI TGC'GCA
Cuts at: 4974 4974
Size: 7193
HaeII r_GCGC'y
Cuts at: 4627 4875 5897 6876 4627
Size: 248 1022 979 4944
Fragments arranged by size:
4944 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 296 328 1306 1338 2293 2325 2362 2394 3145
Size: 32 978 32 955 32 37 32 751
Cuts at: 3145 3157 3177 3189 3450 3482 3910 3942 5035
Size: 12 20 12 261 32 428 32 1093
Cuts at: 5035 5065 5067 5097 296
Size: 30 2 30 2392
Fragments arranged by size:
2392 1093 978 955 751 428 261 37
32 32 32 32 32 32 30 30
20 12 12 2
HincII GTy'rAC
Cuts at: 147 1629 2031 2483 3906 4098 4232 147
Size: 1482 402 452 1423 192 134 3108
Fragments arranged by size:
3108 1482 1423 452 402 192 134
HindIII A'AGCT_T
Cuts at: 2675 3437 2675
Size: 762 6431
HpaI GTT'AAC
Cuts at: 147 147
Size: 7193
KasI G'GCGC_C
Cuts at: 4623 4871 4623
Size: 248 6945
KpnI G_GTAC'C
Cuts at: 4213 4213
Size: 7193
MluI A'CGCG_T
Cuts at: 6626 6626
Size: 7193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 434 1521 2319 3668 5864 6048 434
Size: 1087 798 1349 2196 184 1579
Fragments arranged by size:
2196 1579 1349 1087 798 184
MscI TGG'CCA
Cuts at: 679 928 2357 4954 679
Size: 249 1429 2597 2918
Fragments arranged by size:
2918 2597 1429 249
MslI CAynn'nnrTG
Cuts at: 643 877 979 1363 2116 5182 5309 643
Size: 234 102 384 753 3066 127 2527
Fragments arranged by size:
3066 2527 753 384 234 127 102
MspA1I CmG'CkG
Cuts at: 543 808 1038 1119 1280 1402 2383 3010 3745
Size: 265 230 81 161 122 981 627 735
Cuts at: 3745 4304 4618 4978 5991 6236 543
Size: 559 314 360 1013 245 1500
Fragments arranged by size:
1500 1013 981 735 627 559 360 314
265 245 230 161 122 81
NaeI GCC'GGC
Cuts at: 5374 5374
Size: 7193
NarI GG'CG_CC
Cuts at: 4624 4872 4624
Size: 248 6945
NcoI C'CATG_G
Cuts at: 125 827 3527 125
Size: 702 2700 3791
Fragments arranged by size:
3791 2700 702
NgoMIV G'CCGG_C
Cuts at: 5372 5372
Size: 7193
NsiI A_TGCA'T
Cuts at: 1859 3024 1859
Size: 1165 6028
NspI r_CATG'y
Cuts at: 1248 3026 3404 3768 4246 4746 5653 1248
Size: 1778 378 364 478 500 907 2788
Fragments arranged by size:
2788 1778 907 500 478 378 364
PciI A'CATG_T
Cuts at: 5649 5649
Size: 7193
PflMI CCAn_nnn'nTGG
Cuts at: 2259 2259
Size: 7193
PfoI T'CCnGG_A
Cuts at: 1268 3911 1268
Size: 2643 4550
PmeI GTTT'AAAC
Cuts at: 4192 4192
Size: 7193
PmlI CAC'GTG
Cuts at: 1733 3963 1733
Size: 2230 4963
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 6332 6364 114
Size: 32 6186 32 943
Fragments arranged by size:
6186 943 32 32
PpuMI rG'GwC_Cy
Cuts at: 1385 1742 2915 1385
Size: 357 1173 5663
Fragments arranged by size:
5663 1173 357
PspOMI G'GGCC_C
Cuts at: 1136 1136
Size: 7193
PstI C_TGCA'G
Cuts at: 908 1604 2133 4240 4925 908
Size: 696 529 2107 685 3176
Fragments arranged by size:
3176 2107 696 685 529
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7193
PvuII CAG'CTG
Cuts at: 543 808 1038 1119 1280 1402 2383 4618 4978
Size: 265 230 81 161 122 981 2235 360
Cuts at: 4978 543
Size: 2758
Fragments arranged by size:
2758 2235 981 360 265 230 161 122
81
RsrII CG'GwC_CG
Cuts at: 5388 5388
Size: 7193
SacI G_AGCT'C
Cuts at: 838 862 1301 1496 4207 838
Size: 24 439 195 2711 3824
Fragments arranged by size:
3824 2711 439 195 24
SalI G'TCGA_C
Cuts at: 4230 4230
Size: 7193
SapI GCTCTTCn'nnn_
Cuts at: 2768 3180 5216 5426 2768
Size: 412 2036 210 4535
Fragments arranged by size:
4535 2036 412 210
SbfI CC_TGCA'GG
Cuts at: 4240 4240
Size: 7193
ScaI AGT'ACT
Cuts at: 6710 6710
Size: 7193
SexAI A'CCwGG_T
Cuts at: 1721 1721
Size: 7193
SfcI C'TryA_G
Cuts at: 33 904 1095 1403 1600 2129 2279 2459 2534
Size: 871 191 308 197 529 150 180 75
Cuts at: 2534 2658 4236 4921 5914 6105 33
Size: 124 1578 685 993 191 1121
Fragments arranged by size:
1578 1121 993 871 685 529 308 197
191 191 180 150 124 75
SfoI GGC'GCC
Cuts at: 4625 4873 4625
Size: 248 6945
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7193
SmaI CCC'GGG
Cuts at: 1135 2068 4215 1135
Size: 933 2147 4113
Fragments arranged by size:
4113 2147 933
SmlI C'TyrA_G
Cuts at: 1972 2564 2877 4348 5263 5755 6017 1972
Size: 592 313 1471 915 492 262 3148
Fragments arranged by size:
3148 1471 915 592 492 313 262
SnaBI TAC'GTA
Cuts at: 4185 4185
Size: 7193
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7193
SphI G_CATG'C
Cuts at: 3404 4246 4746 3404
Size: 842 500 5851
Fragments arranged by size:
5851 842 500
SspI AAT'ATT
Cuts at: 2421 3288 2421
Size: 867 6326
StuI AGG'CCT
Cuts at: 1288 1288
Size: 7193
StyI C'CwwG_G
Cuts at: 125 774 827 3527 4129 4327 4681 125
Size: 649 53 2700 602 198 354 2637
Fragments arranged by size:
2700 2637 649 602 354 198 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1324 1579 1877 2266 4177 4776 4909 5368 5577
Size: 255 298 389 1911 599 133 459 209
Cuts at: 5577 1324
Size: 2940
Fragments arranged by size:
2940 1911 599 459 389 298 255 209
133
TatI w'GTAC_w
Cuts at: 1545 3025 3040 6708 1545
Size: 1480 15 3668 2030
Fragments arranged by size:
3668 2030 1480 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 548 580 1363 1395 6710 6742 548
Size: 32 783 32 5315 32 999
Fragments arranged by size:
5315 999 783 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1360 2325 4990 1360
Size: 965 2665 3563
Fragments arranged by size:
3563 2665 965
XbaI T'CTAG_A
Cuts at: 4224 4224
Size: 7193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 597 763 825 1698 3007 3525 4688 597
Size: 166 62 873 1309 518 1163 3102
Fragments arranged by size:
3102 1309 1163 873 518 166 62
XmaI C'CCGG_G
Cuts at: 1133 2066 4213 1133
Size: 933 2147 4113
Fragments arranged by size:
4113 2147 933
ZraI GAC'GTC
Cuts at: 4148 4148
Size: 7193
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AloI AscI BcgI BmtI BsiWI
BssHII BstZ17I ClaI EcoNI EcoRV FseI FspAI MfeI
NdeI NheI NotI NruI PacI PasI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SrfI SwaI
XhoI XmnI