Restriction map for
pF1KSDA0171
(Circular) (Six-base) MAPSORT of: pF1KSDA0171.seq Check: 8165 from: 1 to: 5012
pF1KSDA0171 5012 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 2050 2050
Size: 5012
Acc65I G'GTAC_C
Cuts at: 2028 2028
Size: 5012
AclI AA'CG_TT
Cuts at: 4852 4852
Size: 5012
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1043 1055 1370 2837 3269 4016 4284 1043
Size: 12 315 1467 432 747 268 1771
Fragments arranged by size:
1771 1467 747 432 315 268 12
AflIII A'CryG_T
Cuts at: 134 2364 3468 4445 134
Size: 2230 1104 977 701
Fragments arranged by size:
2230 1104 977 701
AgeI A'CCGG_T
Cuts at: 3400 3400
Size: 5012
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1558 1558
Size: 5012
AlwNI CAG_nnn'CTG
Cuts at: 1186 1279 2317 3884 1186
Size: 93 1038 1567 2314
Fragments arranged by size:
2314 1567 1038 93
ApaLI G'TGCA_C
Cuts at: 3782 3782
Size: 5012
ApoI r'AATT_y
Cuts at: 535 871 1380 1446 1980 2016 4365 4621 535
Size: 336 509 66 534 36 2349 256 926
Fragments arranged by size:
2349 926 534 509 336 256 66 36
AseI AT'TA_AT
Cuts at: 20 20
Size: 5012
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5012
AvaI C'yCGr_G
Cuts at: 2032 2032
Size: 5012
BamHI G'GATC_C
Cuts at: 2037 2037
Size: 5012
BanI G'GyrC_C
Cuts at: 1120 1801 2028 2442 2690 2725 1120
Size: 681 227 414 248 35 3407
Fragments arranged by size:
3407 681 414 248 227 35
BanII G_rGCy'C
Cuts at: 665 1411 2026 3056 665
Size: 746 615 1030 2621
Fragments arranged by size:
2621 1030 746 615
BbeI G_GCGC'C
Cuts at: 2446 2694 2446
Size: 248 4764
BbsI GAAGACnn'nnnn_
Cuts at: 806 1049 806
Size: 243 4769
BciVI GTATCCnnnnn_n'
Cuts at: 2910 3671 5012 2910
Size: 761 1341 2910
Fragments arranged by size:
2910 1341 761
BclI T'GATC_A
Cuts at: 2532 2532
Size: 5012
BglII A'GATC_T
Cuts at: 856 2527 4564 856
Size: 1671 2037 1304
Fragments arranged by size:
2037 1671 1304
BlpI GC'TnA_GC
Cuts at: 1179 2124 1179
Size: 945 4067
Bme1580I G_kGCm'C
Cuts at: 2637 2730 3786 2637
Size: 93 1056 3863
Fragments arranged by size:
3863 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1559 2224 2305 2640 4784 1559
Size: 665 81 335 2144 1787
Fragments arranged by size:
2144 1787 665 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3379 3379
Size: 5012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1269 791
Size: 478 4534
Bpu10I CC'TnA_GC
Cuts at: 659 659
Size: 5012
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2188 3067 3559 3857 2188
Size: 879 492 298 3343
Fragments arranged by size:
3343 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 925 4716 925
Size: 3791 1221
BsaAI yAC'GTr
Cuts at: 2004 2365 2004
Size: 361 4651
BsaBI GATnn'nnATC
Cuts at: 6 2526 4563 6
Size: 2520 2037 455
Fragments arranged by size:
2520 2037 455
BsaHI Gr'CG_yC
Cuts at: 2443 2691 3393 4889 2443
Size: 248 702 1496 2566
Fragments arranged by size:
2566 1496 702 248
BsaWI w'CCGG_w
Cuts at: 782 2201 2722 3400 3674 3821 4232 782
Size: 1419 521 678 274 147 411 1562
Fragments arranged by size:
1562 1419 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 697 901 697
Size: 204 4808
BseYI C'CCAG_C
Cuts at: 1619 1888 2437 3195 3772 4268 1619
Size: 269 549 758 577 496 2363
Fragments arranged by size:
2363 758 577 549 496 269
BsiEI CG_ry'CG
Cuts at: 104 2554 2600 3808 104
Size: 2450 46 1208 1308
Fragments arranged by size:
2450 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1465 2026 2804 2994 3786 1465
Size: 561 778 190 792 2691
Fragments arranged by size:
2691 792 778 561 190
BsmI GAATG_Cn'
Cuts at: 1038 1038
Size: 5012
Bsp1286I G_dGCh'C
Cuts at: 665 1411 1465 2026 2637 2730 2804 2994 3056
Size: 746 54 561 611 93 74 190 62
Cuts at: 3056 3786 665
Size: 730 1891
Fragments arranged by size:
1891 746 730 611 561 190 93 74
62 54
BspEI T'CCGG_A
Cuts at: 782 4232 782
Size: 3450 1562
BspHI T'CATG_A
Cuts at: 4188 4188
Size: 5012
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2062 2276 2578 2959 2062
Size: 214 302 381 4115
Fragments arranged by size:
4115 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3304 3358 4842 5007 50
Size: 3254 54 1484 165 55
Fragments arranged by size:
3254 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2924 2924
Size: 5012
BsrFI r'CCGG_y
Cuts at: 3010 3191 3400 3010
Size: 181 209 4622
Fragments arranged by size:
4622 209 181
BssSI C'ACGA_G
Cuts at: 3283 3641 3283
Size: 358 4654
BstAPI GCAn_nnn'nTGC
Cuts at: 2389 2389
Size: 5012
BstBI TT'CG_AA
Cuts at: 110 3373 110
Size: 3263 1749
BstEII G'GTnAC_C
Cuts at: 1096 1096
Size: 5012
BstXI CCAn_nnnn'nTGG
Cuts at: 1812 1812
Size: 5012
BstYI r'GATC_y
Cuts at: 1 856 2037 2504 2527 2862 3108 4120 4206
Size: 855 1181 467 23 335 246 1012 86
Cuts at: 4206 4218 4564 1
Size: 12 346 449
Fragments arranged by size:
1181 1012 855 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1805 1805
Size: 5012
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 842 2962 3143 3306 842
Size: 2120 181 163 2548
Fragments arranged by size:
2548 2120 181 163
BtsI GCAGTG_nn'
Cuts at: 1314 2255 1314
Size: 941 4071
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 156 191 156
Size: 35 4977
DraI TTT'AAA
Cuts at: 2011 2011
Size: 5012
DrdI GACnn_nn'nnGTC
Cuts at: 2718 3576 2718
Size: 858 4154
EaeI y'GGCC_r
Cuts at: 2597 2771 3162 3189 4534 2597
Size: 174 391 27 1345 3075
Fragments arranged by size:
3075 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2597 2597
Size: 5012
EarI CTCTTCn'nnn_
Cuts at: 351 560 843 878 3035 3245 351
Size: 209 283 35 2157 210 2118
Fragments arranged by size:
2157 2118 283 210 209 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3220 3530 3676 4822 3220
Size: 310 146 1146 3410
Fragments arranged by size:
3410 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2024 2024
Size: 5012
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1043 1055 1269 1370 2837 3269 4016 4284
Size: 252 12 214 101 1467 432 747 268
Cuts at: 4284 791
Size: 1519
Fragments arranged by size:
1519 1467 747 432 268 252 214 101
12
EcoO109I rG'GnC_Cy
Cuts at: 238 1276 1765 2151 238
Size: 1038 489 386 3099
Fragments arranged by size:
3099 1038 489 386
EcoRI G'AATT_C
Cuts at: 871 2016 871
Size: 1145 3867
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 5012
FspI TGC'GCA
Cuts at: 2793 2793
Size: 5012
HaeII r_GCGC'y
Cuts at: 2446 2694 3716 4695 2446
Size: 248 1022 979 2763
Fragments arranged by size:
2763 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 436 468 652 684 1162 1194 2854
Size: 32 220 32 184 32 478 32 1660
Cuts at: 2854 2884 2886 2916 184
Size: 30 2 30 2280
Fragments arranged by size:
2280 1660 478 220 184 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1133 2051 1133
Size: 918 4094
KasI G'GCGC_C
Cuts at: 2442 2690 2442
Size: 248 4764
KpnI G_GTAC'C
Cuts at: 2032 2032
Size: 5012
MfeI C'AATT_G
Cuts at: 758 758
Size: 5012
MluI A'CGCG_T
Cuts at: 4445 4445
Size: 5012
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 606 3683 3867 606
Size: 3077 184 1751
Fragments arranged by size:
3077 1751 184
MscI TGG'CCA
Cuts at: 2773 2773
Size: 5012
MslI CAynn'nnrTG
Cuts at: 440 1774 1810 1864 1876 1936 3001 3128 440
Size: 1334 36 54 12 60 1065 127 2324
Fragments arranged by size:
2324 1334 1065 127 60 54 36 12
MspA1I CmG'CkG
Cuts at: 1147 1183 1324 1885 2123 2437 2797 3810 4055
Size: 36 141 561 238 314 360 1013 245
Cuts at: 4055 1147
Size: 2104
Fragments arranged by size:
2104 1013 561 360 314 245 238 141
36
NaeI GCC'GGC
Cuts at: 3193 3193
Size: 5012
NarI GG'CG_CC
Cuts at: 2443 2691 2443
Size: 248 4764
NcoI C'CATG_G
Cuts at: 1805 1805
Size: 5012
NgoMIV G'CCGG_C
Cuts at: 3191 3191
Size: 5012
NspI r_CATG'y
Cuts at: 138 315 1728 1785 1926 2065 2565 3472 138
Size: 177 1413 57 141 139 500 907 1678
Fragments arranged by size:
1678 1413 907 500 177 141 139 57
PciI A'CATG_T
Cuts at: 134 3468 134
Size: 3334 1678
PmeI GTTT'AAAC
Cuts at: 2011 2011
Size: 5012
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4151 4183 4151
Size: 32 4980
PpuMI rG'GwC_Cy
Cuts at: 238 1765 238
Size: 1527 3485
PsiI TTA'TAA
Cuts at: 365 365
Size: 5012
PstI C_TGCA'G
Cuts at: 2059 2744 2059
Size: 685 4327
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5012
PvuII CAG'CTG
Cuts at: 1147 1183 2437 2797 1147
Size: 36 1254 360 3362
Fragments arranged by size:
3362 1254 360 36
RsrII CG'GwC_CG
Cuts at: 3207 3207
Size: 5012
SacI G_AGCT'C
Cuts at: 2026 2026
Size: 5012
SalI G'TCGA_C
Cuts at: 2049 2049
Size: 5012
SanDI GG'GwC_CC
Cuts at: 1765 1765
Size: 5012
SapI GCTCTTCn'nnn_
Cuts at: 560 3035 3245 560
Size: 2475 210 2327
Fragments arranged by size:
2475 2327 210
SbfI CC_TGCA'GG
Cuts at: 2059 2059
Size: 5012
ScaI AGT'ACT
Cuts at: 4529 4529
Size: 5012
SexAI A'CCwGG_T
Cuts at: 1596 1596
Size: 5012
SfcI C'TryA_G
Cuts at: 33 2055 2740 3733 3924 33
Size: 2022 685 993 191 1121
Fragments arranged by size:
2022 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2444 2692 2444
Size: 248 4764
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5012
SmaI CCC'GGG
Cuts at: 2034 2034
Size: 5012
SmlI C'TyrA_G
Cuts at: 2167 3082 3574 3836 2167
Size: 915 492 262 3343
Fragments arranged by size:
3343 915 492 262
SnaBI TAC'GTA
Cuts at: 2004 2004
Size: 5012
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5012
SphI G_CATG'C
Cuts at: 1785 1926 2065 2565 1785
Size: 141 139 500 4232
Fragments arranged by size:
4232 500 141 139
SspI AAT'ATT
Cuts at: 507 507
Size: 5012
StyI C'CwwG_G
Cuts at: 232 269 1805 2146 2500 232
Size: 37 1536 341 354 2744
Fragments arranged by size:
2744 1536 354 341 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2595 2728 3187 3396 2595
Size: 133 459 209 4211
Fragments arranged by size:
4211 459 209 133
TatI w'GTAC_w
Cuts at: 4527 4527
Size: 5012
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 670 702 948 980 4529 4561 670
Size: 32 246 32 3549 32 1121
Fragments arranged by size:
3549 1121 246 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2809 2809
Size: 5012
XbaI T'CTAG_A
Cuts at: 2043 2043
Size: 5012
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2507 2507
Size: 5012
XmaI C'CCGG_G
Cuts at: 2032 2032
Size: 5012
XmnI GAAnn'nnTTC
Cuts at: 293 293
Size: 5012
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PspOMI PspXI PsrI SacII
SfiI SgrAI SrfI StuI SwaI XhoI ZraI