Restriction map for
pF1KSDA0155
(Circular) (Six-base) MAPSORT of: pF1KSDA0155.seq Check: 5054 from: 1 to: 6656
pF1KSDA0155 6656 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AatII G_ACGT'C
Cuts at: 3081 3081
Size: 6656
AccI GT'mk_AC
Cuts at: 475 3694 475
Size: 3219 3437
Acc65I G'GTAC_C
Cuts at: 3672 3672
Size: 6656
AclI AA'CG_TT
Cuts at: 6496 6496
Size: 6656
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1432 1760 1783 2297 2368 2473 2487 2831 4481
Size: 328 23 514 71 105 14 344 1650
Cuts at: 4481 4913 5660 5928 1432
Size: 432 747 268 2160
Fragments arranged by size:
2160 1650 747 514 432 344 328 268
105 71 23 14
AflIII A'CryG_T
Cuts at: 1952 2358 4008 5112 6089 1952
Size: 406 1650 1104 977 2519
Fragments arranged by size:
2519 1650 1104 977 406
AgeI A'CCGG_T
Cuts at: 5044 5044
Size: 6656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1065 1227 1065
Size: 162 6494
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 827 859 827
Size: 32 6624
AlwNI CAG_nnn'CTG
Cuts at: 996 3961 5528 996
Size: 2965 1567 2124
Fragments arranged by size:
2965 2124 1567
ApaI G_GGCC'C
Cuts at: 2304 2763 2304
Size: 459 6197
ApaLI G'TGCA_C
Cuts at: 5426 5426
Size: 6656
ApoI r'AATT_y
Cuts at: 1262 1660 2881 3660 6009 6265 1262
Size: 398 1221 779 2349 256 1653
Fragments arranged by size:
2349 1653 1221 779 398 256
AseI AT'TA_AT
Cuts at: 20 1805 20
Size: 1785 4871
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6656
AvaI C'yCGr_G
Cuts at: 1982 2297 2612 3676 1982
Size: 315 315 1064 4962
Fragments arranged by size:
4962 1064 315 315
AvrII C'CTAG_G
Cuts at: 1524 1734 1524
Size: 210 6446
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2490 2523 2490
Size: 33 6623
BamHI G'GATC_C
Cuts at: 3589 3681 3589
Size: 92 6564
BanI G'GyrC_C
Cuts at: 1411 1498 2364 3672 4086 4334 4369 1411
Size: 87 866 1308 414 248 35 3698
Fragments arranged by size:
3698 1308 866 414 248 87 35
BanII G_rGCy'C
Cuts at: 139 672 798 2304 2763 3670 4700 139
Size: 533 126 1506 459 907 1030 2095
Fragments arranged by size:
2095 1506 1030 907 533 459 126
BbeI G_GCGC'C
Cuts at: 4090 4338 4090
Size: 248 6408
BbsI GAAGACnn'nnnn_
Cuts at: 2782 3015 3363 2782
Size: 233 348 6075
Fragments arranged by size:
6075 348 233
BciVI GTATCCnnnnn_n'
Cuts at: 378 1358 4554 5315 6656 378
Size: 980 3196 761 1341 378
Fragments arranged by size:
3196 1341 980 761 378
BclI T'GATC_A
Cuts at: 504 4176 504
Size: 3672 2984
BfrBI ATG'CAT
Cuts at: 1009 1204 1009
Size: 195 6461
BglII A'GATC_T
Cuts at: 4171 6208 4171
Size: 2037 4619
BlpI GC'TnA_GC
Cuts at: 3768 3768
Size: 6656
Bme1580I G_kGCm'C
Cuts at: 1416 1503 2304 2619 2763 4281 4374 5430 1416
Size: 87 801 315 144 1518 93 1056 2642
Fragments arranged by size:
2642 1518 1056 801 315 144 93 87
BmgBI CAC'GTC
Cuts at: 1622 1622
Size: 6656
BmrI ACTGGGnnnn_n'
Cuts at: 2363 2592 2757 3868 3949 4284 6428 2363
Size: 229 165 1111 81 335 2144 2591
Fragments arranged by size:
2591 2144 1111 335 229 165 81
BmtI G_CTAG'C
Cuts at: 1063 1942 1063
Size: 879 5777
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3501 5023 3501
Size: 1522 5134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1460 2503 1460
Size: 1043 5613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 3832 4711 5203 5501 565
Size: 3267 879 492 298 1720
Fragments arranged by size:
3267 1720 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 347 6360 347
Size: 6013 643
BsaAI yAC'GTr
Cuts at: 328 3648 4009 328
Size: 3320 361 2975
Fragments arranged by size:
3320 2975 361
BsaBI GATnn'nnATC
Cuts at: 6 4170 6207 6
Size: 4164 2037 455
Fragments arranged by size:
4164 2037 455
BsaHI Gr'CG_yC
Cuts at: 1552 3078 4087 4335 5037 6533 1552
Size: 1526 1009 248 702 1496 1675
Fragments arranged by size:
1675 1526 1496 1009 702 248
BsaWI w'CCGG_w
Cuts at: 198 3415 3845 4366 5044 5318 5465 5876 198
Size: 3217 430 521 678 274 147 411 978
Fragments arranged by size:
3217 978 678 521 430 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 197 227 815 845 197
Size: 30 588 30 6008
Fragments arranged by size:
6008 588 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 680 2882 2989 3018 680
Size: 2202 107 29 4318
Fragments arranged by size:
4318 2202 107 29
BseYI C'CCAG_C
Cuts at: 3492 4081 4839 5416 5912 3492
Size: 589 758 577 496 4236
Fragments arranged by size:
4236 758 589 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 1480 3466 232
Size: 1248 1986 3422
Fragments arranged by size:
3422 1986 1248
BsiEI CG_ry'CG
Cuts at: 104 4198 4244 5452 104
Size: 4094 46 1208 1308
Fragments arranged by size:
4094 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 672 1151 2022 3670 4448 4638 5430 672
Size: 479 871 1648 778 190 792 1898
Fragments arranged by size:
1898 1648 871 792 778 479 190
BsmI GAATG_Cn'
Cuts at: 784 1009 2335 784
Size: 225 1326 5105
Fragments arranged by size:
5105 1326 225
BsmBI CGTCTCn'nnnn_
Cuts at: 2722 2722
Size: 6656
Bsp1286I G_dGCh'C
Cuts at: 139 672 798 1151 1416 1503 2022 2304 2619
Size: 533 126 353 265 87 519 282 315
Cuts at: 2619 2763 3670 4281 4374 4448 4638 4700 5430
Size: 144 907 611 93 74 190 62 730
Cuts at: 5430 139
Size: 1365
Fragments arranged by size:
1365 907 730 611 533 519 353 315
282 265 190 144 126 93 87 74
62
BspEI T'CCGG_A
Cuts at: 5876 5876
Size: 6656
BspHI T'CATG_A
Cuts at: 5832 5832
Size: 6656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3706 3920 4222 4603 3706
Size: 214 302 381 5759
Fragments arranged by size:
5759 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2640 4948 5002 6486 6651 50
Size: 2590 2308 54 1484 165 55
Fragments arranged by size:
2590 2308 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 691 741 4568 691
Size: 50 3827 2779
Fragments arranged by size:
3827 2779 50
BsrFI r'CCGG_y
Cuts at: 3334 4654 4835 5044 3334
Size: 1320 181 209 4946
Fragments arranged by size:
4946 1320 209 181
BsrGI T'GTAC_A
Cuts at: 405 405
Size: 6656
BssSI C'ACGA_G
Cuts at: 2123 4927 5285 2123
Size: 2804 358 3494
Fragments arranged by size:
3494 2804 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4033 4033
Size: 6656
BstBI TT'CG_AA
Cuts at: 110 5017 110
Size: 4907 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 819 1826 3565 819
Size: 1007 1739 3910
Fragments arranged by size:
3910 1739 1007
BstYI r'GATC_y
Cuts at: 1 447 3031 3535 3589 3681 4148 4171 4506
Size: 446 2584 504 54 92 467 23 335
Cuts at: 4506 4752 5764 5850 5862 6208 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2584 1012 504 467 449 446 346 335
246 92 86 54 23 12
BstZ17I GTA'TAC
Cuts at: 476 476
Size: 6656
BtgI C'CryG_G
Cuts at: 1742 1742
Size: 6656
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1666 4606 4787 4950 1666
Size: 2940 181 163 3372
Fragments arranged by size:
3372 2940 181 163
BtsI GCAGTG_nn'
Cuts at: 2578 2958 3899 2578
Size: 380 941 5335
Fragments arranged by size:
5335 941 380
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1110 1145 1110
Size: 35 6621
DraI TTT'AAA
Cuts at: 3655 3655
Size: 6656
DraIII CAC_nnn'GTG
Cuts at: 1921 2371 1921
Size: 450 6206
DrdI GACnn_nn'nnGTC
Cuts at: 4362 5220 4362
Size: 858 5798
EaeI y'GGCC_r
Cuts at: 482 2024 3555 4241 4415 4806 4833 6178 482
Size: 1542 1531 686 174 391 27 1345 960
Fragments arranged by size:
1542 1531 1345 960 686 391 174 27
EagI C'GGCC_G
Cuts at: 4241 4241
Size: 6656
EarI CTCTTCn'nnn_
Cuts at: 293 519 1802 2311 2630 2702 2726 2926 3189
Size: 226 1283 509 319 72 24 200 263
Cuts at: 3189 4679 4889 293
Size: 1490 210 2060
Fragments arranged by size:
2060 1490 1283 509 319 263 226 210
200 72 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4864 5174 5320 6466 4864
Size: 310 146 1146 5054
Fragments arranged by size:
5054 1146 310 146
EcoICRI GAG'CTC
Cuts at: 670 3668 670
Size: 2998 3658
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1432 1460 1760 1783 2297 2368 2473 2487 2503
Size: 28 300 23 514 71 105 14 16
Cuts at: 2503 2831 4481 4913 5660 5928 1432
Size: 328 1650 432 747 268 2160
Fragments arranged by size:
2160 1650 747 514 432 328 300 268
105 71 28 23 16 14
EcoNI CCTnn'n_nnAGG
Cuts at: 544 1528 544
Size: 984 5672
EcoO109I rG'GnC_Cy
Cuts at: 1870 2301 3410 3795 1870
Size: 431 1109 385 4731
Fragments arranged by size:
4731 1109 431 385
EcoRI G'AATT_C
Cuts at: 1660 3660 1660
Size: 2000 4656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1779 4675 1779
Size: 2896 3760
FspI TGC'GCA
Cuts at: 4437 4437
Size: 6656
HaeII r_GCGC'y
Cuts at: 271 2225 4090 4338 5360 6339 271
Size: 1954 1865 248 1022 979 588
Fragments arranged by size:
1954 1865 1022 979 588 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1038 1070 1813 1827 1845 1859 2997 3029 3361
Size: 32 743 14 18 14 1138 32 332
Cuts at: 3361 3379 3393 3411 3424 3456 3508 3520 3540
Size: 18 14 18 13 32 52 12 20
Cuts at: 3540 3552 3601 3633 4498 4528 4530 4560 1038
Size: 12 49 32 865 30 2 30 3134
Fragments arranged by size:
3134 1138 865 743 332 52 49 32
32 32 32 30 30 20 18 18
18 14 14 14 13 12 12 2
HincII GTy'rAC
Cuts at: 1719 3695 1719
Size: 1976 4680
HindIII A'AGCT_T
Cuts at: 632 1097 1232 1364 632
Size: 465 135 132 5924
Fragments arranged by size:
5924 465 135 132
KasI G'GCGC_C
Cuts at: 4086 4334 4086
Size: 248 6408
KpnI G_GTAC'C
Cuts at: 3676 3676
Size: 6656
MluI A'CGCG_T
Cuts at: 6089 6089
Size: 6656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 296 813 3617 5327 5511 296
Size: 517 2804 1710 184 1441
Fragments arranged by size:
2804 1710 1441 517 184
MscI TGG'CCA
Cuts at: 2026 3557 4417 2026
Size: 1531 860 4265
Fragments arranged by size:
4265 1531 860
MslI CAynn'nnrTG
Cuts at: 1209 1820 2195 2197 4645 4772 1209
Size: 611 375 2 2448 127 3093
Fragments arranged by size:
3093 2448 611 375 127 2
MspA1I CmG'CkG
Cuts at: 718 2757 3363 3767 4081 4441 5454 5699 718
Size: 2039 606 404 314 360 1013 245 1675
Fragments arranged by size:
2039 1675 1013 606 404 360 314 245
NaeI GCC'GGC
Cuts at: 3336 4837 3336
Size: 1501 5155
NarI GG'CG_CC
Cuts at: 4087 4335 4087
Size: 248 6408
NcoI C'CATG_G
Cuts at: 1742 1742
Size: 6656
NdeI CA'TA_TG
Cuts at: 253 253
Size: 6656
NgoMIV G'CCGG_C
Cuts at: 3334 4835 3334
Size: 1501 5155
NheI G'CTAG_C
Cuts at: 1059 1938 1059
Size: 879 5777
NsiI A_TGCA'T
Cuts at: 1011 1206 1011
Size: 195 6461
NspI r_CATG'y
Cuts at: 2362 3709 4209 5116 2362
Size: 1347 500 907 3902
Fragments arranged by size:
3902 1347 907 500
PciI A'CATG_T
Cuts at: 2358 5112 2358
Size: 2754 3902
PflMI CCAn_nnn'nTGG
Cuts at: 519 996 1159 2769 519
Size: 477 163 1610 4406
Fragments arranged by size:
4406 1610 477 163
PfoI T'CCnGG_A
Cuts at: 155 155
Size: 6656
PmeI GTTT'AAAC
Cuts at: 3655 3655
Size: 6656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5795 5827 5795
Size: 32 6624
PpuMI rG'GwC_Cy
Cuts at: 1870 1870
Size: 6656
PshAI GACnn'nnGTC
Cuts at: 3363 3363
Size: 6656
PspOMI G'GGCC_C
Cuts at: 2300 2759 2300
Size: 459 6197
PstI C_TGCA'G
Cuts at: 3703 4388 3703
Size: 685 5971
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6656
PvuII CAG'CTG
Cuts at: 4081 4441 4081
Size: 360 6296
RsrII CG'GwC_CG
Cuts at: 4851 4851
Size: 6656
SacI G_AGCT'C
Cuts at: 672 3670 672
Size: 2998 3658
SalI G'TCGA_C
Cuts at: 3693 3693
Size: 6656
SapI GCTCTTCn'nnn_
Cuts at: 519 1802 2630 2726 4679 4889 519
Size: 1283 828 96 1953 210 2286
Fragments arranged by size:
2286 1953 1283 828 210 96
SbfI CC_TGCA'GG
Cuts at: 3703 3703
Size: 6656
ScaI AGT'ACT
Cuts at: 1108 2042 2462 6173 1108
Size: 934 420 3711 1591
Fragments arranged by size:
3711 1591 934 420
SfcI C'TryA_G
Cuts at: 33 348 2558 3699 4384 5377 5568 33
Size: 315 2210 1141 685 993 191 1121
Fragments arranged by size:
2210 1141 1121 993 685 315 191
SfoI GGC'GCC
Cuts at: 4088 4336 4088
Size: 248 6408
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6656
SmaI CCC'GGG
Cuts at: 2299 2614 3678 2299
Size: 315 1064 5277
Fragments arranged by size:
5277 1064 315
SmlI C'TyrA_G
Cuts at: 544 3811 4726 5218 5480 544
Size: 3267 915 492 262 1720
Fragments arranged by size:
3267 1720 915 492 262
SnaBI TAC'GTA
Cuts at: 3648 3648
Size: 6656
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6656
SphI G_CATG'C
Cuts at: 3709 4209 3709
Size: 500 6156
SrfI GCCC'GGGC
Cuts at: 2299 2614 2299
Size: 315 6341
SspI AAT'ATT
Cuts at: 612 1543 2787 612
Size: 931 1244 4481
Fragments arranged by size:
4481 1244 931
StuI AGG'CCT
Cuts at: 3604 3604
Size: 6656
StyI C'CwwG_G
Cuts at: 1316 1524 1734 1742 1944 2399 3558 3790 4144
Size: 208 210 8 202 455 1159 232 354
Cuts at: 4144 1316
Size: 3828
Fragments arranged by size:
3828 1159 455 354 232 210 208 202
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1486 2382 4239 4372 4831 5040 1486
Size: 896 1857 133 459 209 3102
Fragments arranged by size:
3102 1857 896 459 209 133
TatI w'GTAC_w
Cuts at: 405 588 1106 2040 2213 2283 2460 6171 405
Size: 183 518 934 173 70 177 3711 890
Fragments arranged by size:
3711 934 890 518 183 177 173 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6173 6205 6173
Size: 32 6624
Tth111I GACn'n_nGTC
Cuts at: 169 4453 169
Size: 4284 2372
XbaI T'CTAG_A
Cuts at: 846 3687 846
Size: 2841 3815
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1513 3319 4151 1513
Size: 1806 832 4018
Fragments arranged by size:
4018 1806 832
XmaI C'CCGG_G
Cuts at: 2297 2612 3676 2297
Size: 315 1064 5277
Fragments arranged by size:
5277 1064 315
XmnI GAAnn'nnTTC
Cuts at: 1796 1796
Size: 6656
ZraI GAC'GTC
Cuts at: 3079 3079
Size: 6656
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AscI BbvCI BcgI
BglI Bpu10I BsiWI BssHII BstEII Bsu36I ClaI EcoRV
FseI FspAI HpaI MfeI NotI NruI PacI PasI
PmlI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SwaI XhoI