Restriction map for
pF1KSDA0152
(Circular) (Six-base) MAPSORT of: pF1KSDA0152.seq Check: 7432 from: 1 to: 4013
pF1KSDA0152 4013 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 515 1051 515
Size: 536 3477
Acc65I G'GTAC_C
Cuts at: 1029 1029
Size: 4013
AclI AA'CG_TT
Cuts at: 3853 3853
Size: 4013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1838 2270 3017 3285 772
Size: 1066 432 747 268 1500
Fragments arranged by size:
1500 1066 747 432 268
AflIII A'CryG_T
Cuts at: 1365 2469 3446 1365
Size: 1104 977 1932
Fragments arranged by size:
1932 1104 977
AgeI A'CCGG_T
Cuts at: 2401 2401
Size: 4013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 397 397
Size: 4013
AlwNI CAG_nnn'CTG
Cuts at: 417 1318 2885 417
Size: 901 1567 1545
Fragments arranged by size:
1567 1545 901
ApaLI G'TGCA_C
Cuts at: 310 743 2783 310
Size: 433 2040 1540
Fragments arranged by size:
2040 1540 433
ApoI r'AATT_y
Cuts at: 502 1017 3366 3622 502
Size: 515 2349 256 893
Fragments arranged by size:
2349 893 515 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
AvaI C'yCGr_G
Cuts at: 211 259 1033 211
Size: 48 774 3191
Fragments arranged by size:
3191 774 48
BamHI G'GATC_C
Cuts at: 317 1038 317
Size: 721 3292
BanI G'GyrC_C
Cuts at: 1029 1443 1691 1726 1029
Size: 414 248 35 3316
Fragments arranged by size:
3316 414 248 35
BanII G_rGCy'C
Cuts at: 218 1027 2057 218
Size: 809 1030 2174
Fragments arranged by size:
2174 1030 809
BbeI G_GCGC'C
Cuts at: 1447 1695 1447
Size: 248 3765
BbsI GAAGACnn'nnnn_
Cuts at: 960 960
Size: 4013
BciVI GTATCCnnnnn_n'
Cuts at: 1911 2672 4013 1911
Size: 761 1341 1911
Fragments arranged by size:
1911 1341 761
BclI T'GATC_A
Cuts at: 1533 1533
Size: 4013
BglI GCCn_nnn'nGGC
Cuts at: 232 241 353 232
Size: 9 112 3892
Fragments arranged by size:
3892 112 9
BglII A'GATC_T
Cuts at: 1528 3565 1528
Size: 2037 1976
BlpI GC'TnA_GC
Cuts at: 1125 1125
Size: 4013
Bme1580I G_kGCm'C
Cuts at: 314 467 747 1638 1731 2787 314
Size: 153 280 891 93 1056 1540
Fragments arranged by size:
1540 1056 891 280 153 93
BmgBI CAC'GTC
Cuts at: 309 567 309
Size: 258 3755
BmrI ACTGGGnnnn_n'
Cuts at: 1225 1306 1641 3785 1225
Size: 81 335 2144 1453
Fragments arranged by size:
2144 1453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4013
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1189 2068 2560 2858 1189
Size: 879 492 298 2344
Fragments arranged by size:
2344 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 439 3717 439
Size: 3278 735
BsaAI yAC'GTr
Cuts at: 489 1005 1366 489
Size: 516 361 3136
Fragments arranged by size:
3136 516 361
BsaBI GATnn'nnATC
Cuts at: 6 1527 3564 6
Size: 1521 2037 455
Fragments arranged by size:
2037 1521 455
BsaHI Gr'CG_yC
Cuts at: 1444 1692 2394 3890 1444
Size: 248 702 1496 1567
Fragments arranged by size:
1567 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1202 1723 2401 2675 2822 3233 1202
Size: 521 678 274 147 411 1982
Fragments arranged by size:
1982 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 457 490 457
Size: 33 3980
BseYI C'CCAG_C
Cuts at: 129 530 760 1438 2196 2773 3269 129
Size: 401 230 678 758 577 496 873
Fragments arranged by size:
873 758 678 577 496 401 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 907
Size: 4013
BsiEI CG_ry'CG
Cuts at: 104 1555 1601 2809 104
Size: 1451 46 1208 1308
Fragments arranged by size:
1451 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 314 747 1027 1805 1995 2787 314
Size: 433 280 778 190 792 1540
Fragments arranged by size:
1540 792 778 433 280 190
Bsp1286I G_dGCh'C
Cuts at: 218 314 467 747 1027 1638 1731 1805 1995
Size: 96 153 280 280 611 93 74 190
Cuts at: 1995 2057 2787 218
Size: 62 730 1444
Fragments arranged by size:
1444 730 611 280 280 190 153 96
93 74 62
BspEI T'CCGG_A
Cuts at: 3233 3233
Size: 4013
BspHI T'CATG_A
Cuts at: 3189 3189
Size: 4013
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1063 1277 1579 1960 1063
Size: 214 302 381 3116
Fragments arranged by size:
3116 381 302 214
BsrBI CCG'CTC
Cuts at: 50 432 2305 2359 3843 4008 50
Size: 382 1873 54 1484 165 55
Fragments arranged by size:
1873 1484 382 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1925 1925
Size: 4013
BsrFI r'CCGG_y
Cuts at: 195 226 235 995 2011 2192 2401 195
Size: 31 9 760 1016 181 209 1807
Fragments arranged by size:
1807 1016 760 209 181 31 9
BssSI C'ACGA_G
Cuts at: 2284 2642 2284
Size: 358 3655
BstAPI GCAn_nnn'nTGC
Cuts at: 1390 1390
Size: 4013
BstBI TT'CG_AA
Cuts at: 110 2374 110
Size: 2264 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 954 954
Size: 4013
BstYI r'GATC_y
Cuts at: 1 317 413 1038 1505 1528 1863 2109 3121
Size: 316 96 625 467 23 335 246 1012
Cuts at: 3121 3207 3219 3565 1
Size: 86 12 346 449
Fragments arranged by size:
1012 625 467 449 346 335 316 246
96 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 404 404
Size: 4013
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1963 2144 2307 1963
Size: 181 163 3669
Fragments arranged by size:
3669 181 163
BtsI GCAGTG_nn'
Cuts at: 1256 1256
Size: 4013
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 270 305 270
Size: 35 3978
DraI TTT'AAA
Cuts at: 1012 1012
Size: 4013
DraIII CAC_nnn'GTG
Cuts at: 570 570
Size: 4013
DrdI GACnn_nn'nnGTC
Cuts at: 1719 2577 1719
Size: 858 3155
EaeI y'GGCC_r
Cuts at: 224 233 561 1598 1772 2163 2190 3535 224
Size: 9 328 1037 174 391 27 1345 702
Fragments arranged by size:
1345 1037 702 391 328 174 27 9
EagI C'GGCC_G
Cuts at: 1598 1598
Size: 4013
EarI CTCTTCn'nnn_
Cuts at: 812 988 2036 2246 812
Size: 176 1048 210 2579
Fragments arranged by size:
2579 1048 210 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2221 2531 2677 3823 2221
Size: 310 146 1146 2411
Fragments arranged by size:
2411 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1025 1025
Size: 4013
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1838 2270 3017 3285 772
Size: 1066 432 747 268 1500
Fragments arranged by size:
1500 1066 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 341 341
Size: 4013
EcoO109I rG'GnC_Cy
Cuts at: 208 333 895 1152 208
Size: 125 562 257 3069
Fragments arranged by size:
3069 562 257 125
EcoRI G'AATT_C
Cuts at: 1017 1017
Size: 4013
EcoRV GAT'ATC
Cuts at: 588 588
Size: 4013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 4013
FspI TGC'GCA
Cuts at: 1794 1794
Size: 4013
HaeII r_GCGC'y
Cuts at: 166 1447 1695 2717 3696 166
Size: 1281 248 1022 979 483
Fragments arranged by size:
1281 1022 979 483 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 200 232 405 437 835 867 1855 1885 1887
Size: 32 173 32 398 32 988 30 2
Cuts at: 1887 1917 200
Size: 30 2296
Fragments arranged by size:
2296 988 398 173 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 282 1052 282
Size: 770 3243
HindIII A'AGCT_T
Cuts at: 784 784
Size: 4013
KasI G'GCGC_C
Cuts at: 1443 1691 1443
Size: 248 3765
KpnI G_GTAC'C
Cuts at: 1033 1033
Size: 4013
MluI A'CGCG_T
Cuts at: 3446 3446
Size: 4013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 422 1000 2684 2868 422
Size: 578 1684 184 1567
Fragments arranged by size:
1684 1567 578 184
MscI TGG'CCA
Cuts at: 563 1774 563
Size: 1211 2802
MslI CAynn'nnrTG
Cuts at: 952 2002 2129 952
Size: 1050 127 2836
Fragments arranged by size:
2836 1050 127
MspA1I CmG'CkG
Cuts at: 157 1124 1438 1798 2811 3056 157
Size: 967 314 360 1013 245 1114
Fragments arranged by size:
1114 1013 967 360 314 245
NaeI GCC'GGC
Cuts at: 197 228 237 2194 197
Size: 31 9 1957 2016
Fragments arranged by size:
2016 1957 31 9
NarI GG'CG_CC
Cuts at: 1444 1692 1444
Size: 248 3765
NgoMIV G'CCGG_C
Cuts at: 195 226 235 2192 195
Size: 31 9 1957 2016
Fragments arranged by size:
2016 1957 31 9
NspI r_CATG'y
Cuts at: 304 1066 1566 2473 304
Size: 762 500 907 1844
Fragments arranged by size:
1844 907 762 500
PciI A'CATG_T
Cuts at: 2469 2469
Size: 4013
PflMI CCAn_nnn'nTGG
Cuts at: 570 570
Size: 4013
PfoI T'CCnGG_A
Cuts at: 798 798
Size: 4013
PmeI GTTT'AAAC
Cuts at: 1012 1012
Size: 4013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3184 3152
Size: 32 3981
PpuMI rG'GwC_Cy
Cuts at: 208 333 208
Size: 125 3888
PstI C_TGCA'G
Cuts at: 1060 1745 1060
Size: 685 3328
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4013
PvuII CAG'CTG
Cuts at: 1438 1798 1438
Size: 360 3653
RsrII CG'GwC_CG
Cuts at: 2208 2208
Size: 4013
SacI G_AGCT'C
Cuts at: 1027 1027
Size: 4013
SalI G'TCGA_C
Cuts at: 1050 1050
Size: 4013
SanDI GG'GwC_CC
Cuts at: 208 208
Size: 4013
SapI GCTCTTCn'nnn_
Cuts at: 2036 2246 2036
Size: 210 3803
SbfI CC_TGCA'GG
Cuts at: 1060 1060
Size: 4013
ScaI AGT'ACT
Cuts at: 3530 3530
Size: 4013
SfcI C'TryA_G
Cuts at: 33 1056 1741 2734 2925 33
Size: 1023 685 993 191 1121
Fragments arranged by size:
1121 1023 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 232 353 232
Size: 121 3892
SfoI GGC'GCC
Cuts at: 1445 1693 1445
Size: 248 3765
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4013
SgrAI Cr'CCGG_yG
Cuts at: 195 195
Size: 4013
SmaI CCC'GGG
Cuts at: 213 1035 213
Size: 822 3191
SmlI C'TyrA_G
Cuts at: 1168 2083 2575 2837 1168
Size: 915 492 262 2344
Fragments arranged by size:
2344 915 492 262
SnaBI TAC'GTA
Cuts at: 1005 1005
Size: 4013
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4013
SphI G_CATG'C
Cuts at: 1066 1566 1066
Size: 500 3513
StyI C'CwwG_G
Cuts at: 734 1147 1501 734
Size: 413 354 3246
Fragments arranged by size:
3246 413 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 921 1596 1729 2188 2397 921
Size: 675 133 459 209 2537
Fragments arranged by size:
2537 675 459 209 133
TatI w'GTAC_w
Cuts at: 3528 3528
Size: 4013
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 143 175 645 677 3530 3562 143
Size: 32 470 32 2853 32 594
Fragments arranged by size:
2853 594 470 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1810 1810
Size: 4013
XbaI T'CTAG_A
Cuts at: 1044 1044
Size: 4013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1508 1508
Size: 4013
XmaI C'CCGG_G
Cuts at: 211 1033 211
Size: 822 3191
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I BtgI ClaI FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SrfI
SspI StuI SwaI XhoI XmnI ZraI