Restriction map for
pF1KSDA0146
(Circular) (Six-base) MAPSORT of: pF1KSDA0146.seq Check: 5527 from: 1 to: 5882
pF1KSDA0146 5882 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2630 2630
Size: 5882
AccI GT'mk_AC
Cuts at: 2920 2920
Size: 5882
Acc65I G'GTAC_C
Cuts at: 2056 2898 2056
Size: 842 5040
AclI AA'CG_TT
Cuts at: 5722 5722
Size: 5882
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 347 2125 2264 2335 3707 4139 4886 5154
Size: 63 1778 139 71 1372 432 747 268
Cuts at: 5154 284
Size: 1012
Fragments arranged by size:
1778 1372 1012 747 432 268 139 71
63
AflII C'TTAA_G
Cuts at: 2025 2025
Size: 5882
AflIII A'CryG_T
Cuts at: 2519 3234 4338 5315 2519
Size: 715 1104 977 3086
Fragments arranged by size:
3086 1104 977 715
AgeI A'CCGG_T
Cuts at: 4270 4270
Size: 5882
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1769 1801 2324 2356 1769
Size: 32 523 32 5295
Fragments arranged by size:
5295 523 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1564 3187 4754 1564
Size: 1623 1567 2692
Fragments arranged by size:
2692 1623 1567
ApaLI G'TGCA_C
Cuts at: 1714 4652 1714
Size: 2938 2944
ApoI r'AATT_y
Cuts at: 550 665 876 1031 2886 5235 5491 550
Size: 115 211 155 1855 2349 256 941
Fragments arranged by size:
2349 1855 941 256 211 155 115
AseI AT'TA_AT
Cuts at: 20 20
Size: 5882
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5882
AvaI C'yCGr_G
Cuts at: 146 1667 2588 2902 146
Size: 1521 921 314 3126
Fragments arranged by size:
3126 1521 921 314
BamHI G'GATC_C
Cuts at: 2907 2907
Size: 5882
BanI G'GyrC_C
Cuts at: 2056 2563 2618 2898 3312 3560 3595 2056
Size: 507 55 280 414 248 35 4343
Fragments arranged by size:
4343 507 414 280 248 55 35
BanII G_rGCy'C
Cuts at: 154 433 2242 2896 3926 154
Size: 279 1809 654 1030 2110
Fragments arranged by size:
2110 1809 1030 654 279
BbeI G_GCGC'C
Cuts at: 2567 3316 3564 2567
Size: 749 248 4885
Fragments arranged by size:
4885 749 248
BbsI GAAGACnn'nnnn_
Cuts at: 340 1988 2561 2622 340
Size: 1648 573 61 3600
Fragments arranged by size:
3600 1648 573 61
BbvCI CC'TCA_GC
Cuts at: 2704 2704
Size: 5882
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2476 2510 2476
Size: 34 5848
BciVI GTATCCnnnnn_n'
Cuts at: 3780 4541 5882 3780
Size: 761 1341 3780
Fragments arranged by size:
3780 1341 761
BclI T'GATC_A
Cuts at: 3402 3402
Size: 5882
BglII A'GATC_T
Cuts at: 946 2135 3397 5434 946
Size: 1189 1262 2037 1394
Fragments arranged by size:
2037 1394 1262 1189
BlpI GC'TnA_GC
Cuts at: 2994 2994
Size: 5882
Bme1580I G_kGCm'C
Cuts at: 1469 1718 3507 3600 4656 1469
Size: 249 1789 93 1056 2695
Fragments arranged by size:
2695 1789 1056 249 93
BmrI ACTGGGnnnn_n'
Cuts at: 516 3094 3175 3510 5654 516
Size: 2578 81 335 2144 744
Fragments arranged by size:
2578 2144 744 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 247 1532 2053 4249 247
Size: 1285 521 2196 1880
Fragments arranged by size:
2196 1880 1285 521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 417 854 1154 1586 1904 1994 2023 2143 2272
Size: 437 300 432 318 90 29 120 129
Cuts at: 2272 417
Size: 4027
Fragments arranged by size:
4027 437 432 318 300 129 120 90
29
Bpu10I CC'TnA_GC
Cuts at: 274 1641 2704 274
Size: 1367 1063 3452
Fragments arranged by size:
3452 1367 1063
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2596 2631 3058 3937 4429 4727 2596
Size: 35 427 879 492 298 3751
Fragments arranged by size:
3751 879 492 427 298 35
BsaI GGTCTCn'nnnn_
Cuts at: 235 814 2177 2379 5586 235
Size: 579 1363 202 3207 531
Fragments arranged by size:
3207 1363 579 531 202
BsaAI yAC'GTr
Cuts at: 753 2874 3235 753
Size: 2121 361 3400
Fragments arranged by size:
3400 2121 361
BsaBI GATnn'nnATC
Cuts at: 6 3396 5433 6
Size: 3390 2037 455
Fragments arranged by size:
3390 2037 455
BsaHI Gr'CG_yC
Cuts at: 2564 3313 3561 4263 5759 2564
Size: 749 248 702 1496 2687
Fragments arranged by size:
2687 1496 749 702 248
BsaWI w'CCGG_w
Cuts at: 1205 1332 3071 3592 4270 4544 4691 5102 1205
Size: 127 1739 521 678 274 147 411 1985
Fragments arranged by size:
1985 1739 678 521 411 274 147 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 420 450 1485 1515 1553 1559 1583 1589 420
Size: 30 1035 30 38 6 24 6 4713
Fragments arranged by size:
4713 1035 38 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1098 1907 2191 1098
Size: 809 284 4789
Fragments arranged by size:
4789 809 284
BseYI C'CCAG_C
Cuts at: 1603 1656 1851 2235 2263 3307 4065 4642 5138
Size: 53 195 384 28 1044 758 577 496
Cuts at: 5138 1603
Size: 2347
Fragments arranged by size:
2347 1044 758 577 496 384 195 53
28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 2679 2867 775
Size: 1904 188 3790
Fragments arranged by size:
3790 1904 188
BsiEI CG_ry'CG
Cuts at: 104 3424 3470 4678 104
Size: 3320 46 1208 1308
Fragments arranged by size:
3320 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 433 1718 2494 2896 3674 3864 4656 433
Size: 1285 776 402 778 190 792 1659
Fragments arranged by size:
1659 1285 792 778 776 402 190
BsmI GAATG_Cn'
Cuts at: 187 187
Size: 5882
Bsp1286I G_dGCh'C
Cuts at: 154 433 1469 1718 2242 2494 2896 3507 3600
Size: 279 1036 249 524 252 402 611 93
Cuts at: 3600 3674 3864 3926 4656 154
Size: 74 190 62 730 1380
Fragments arranged by size:
1380 1036 730 611 524 402 279 252
249 190 93 74 62
BspEI T'CCGG_A
Cuts at: 1205 1332 5102 1205
Size: 127 3770 1985
Fragments arranged by size:
3770 1985 127
BspHI T'CATG_A
Cuts at: 5058 5058
Size: 5882
BspMI ACCTGCnnnn'nnnn_
Cuts at: 204 217 2616 2630 2932 3146 3448 3829 204
Size: 13 2399 14 302 214 302 381 2257
Fragments arranged by size:
2399 2257 381 302 302 214 14 13
BsrBI CCG'CTC
Cuts at: 50 2645 4174 4228 5712 5877 50
Size: 2595 1529 54 1484 165 55
Fragments arranged by size:
2595 1529 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3794 3794
Size: 5882
BsrFI r'CCGG_y
Cuts at: 2716 3880 4061 4270 2716
Size: 1164 181 209 4328
Fragments arranged by size:
4328 1164 209 181
BssSI C'ACGA_G
Cuts at: 1551 4153 4511 1551
Size: 2602 358 2922
Fragments arranged by size:
2922 2602 358
BstAPI GCAn_nnn'nTGC
Cuts at: 769 3259 769
Size: 2490 3392
BstBI TT'CG_AA
Cuts at: 110 4243 110
Size: 4133 1749
BstEII G'GTnAC_C
Cuts at: 1091 1091
Size: 5882
BstYI r'GATC_y
Cuts at: 1 798 946 1208 2135 2907 3374 3397 3732
Size: 797 148 262 927 772 467 23 335
Cuts at: 3732 3978 4990 5076 5088 5434 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 927 797 772 467 449 346 335
262 246 148 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1180 1507 1180
Size: 327 5555
BtgI C'CryG_G
Cuts at: 132 1461 2078 132
Size: 1329 617 3936
Fragments arranged by size:
3936 1329 617
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3832 4013 4176 3832
Size: 181 163 5538
Fragments arranged by size:
5538 181 163
BtsI GCAGTG_nn'
Cuts at: 208 2463 2492 2662 3125 208
Size: 2255 29 170 463 2965
Fragments arranged by size:
2965 2255 463 170 29
DraI TTT'AAA
Cuts at: 2881 2881
Size: 5882
DraIII CAC_nnn'GTG
Cuts at: 1844 1844
Size: 5882
DrdI GACnn_nn'nnGTC
Cuts at: 3588 4446 3588
Size: 858 5024
EaeI y'GGCC_r
Cuts at: 1555 1875 3467 3641 4032 4059 5404 1555
Size: 320 1592 174 391 27 1345 2033
Fragments arranged by size:
2033 1592 1345 391 320 174 27
EagI C'GGCC_G
Cuts at: 3467 3467
Size: 5882
EarI CTCTTCn'nnn_
Cuts at: 216 323 1159 1604 1889 2101 2290 2737 3905
Size: 107 836 445 285 212 189 447 1168
Cuts at: 3905 4115 216
Size: 210 1983
Fragments arranged by size:
1983 1168 836 447 445 285 212 210
189 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4090 4400 4546 5692 4090
Size: 310 146 1146 4280
Fragments arranged by size:
4280 1146 310 146
EcoICRI GAG'CTC
Cuts at: 431 2894 431
Size: 2463 3419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 347 417 854 1154 1586 1904 1994 2023
Size: 63 70 437 300 432 318 90 29
Cuts at: 2023 2125 2143 2264 2272 2335 3707 4139 4886
Size: 102 18 121 8 63 1372 432 747
Cuts at: 4886 5154 284
Size: 268 1012
Fragments arranged by size:
1372 1012 747 437 432 432 318 300
268 121 102 90 70 63 63 29
18 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2755 2755
Size: 5882
EcoO109I rG'GnC_Cy
Cuts at: 2855 3021 2855
Size: 166 5716
EcoRI G'AATT_C
Cuts at: 2886 2886
Size: 5882
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1379 3901 1379
Size: 2522 3360
FspI TGC'GCA
Cuts at: 3663 3663
Size: 5882
HaeII r_GCGC'y
Cuts at: 2567 3316 3564 4586 5565 2567
Size: 749 248 1022 979 2884
Fragments arranged by size:
2884 1022 979 749 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 456 488 619 651 664 696 718 750 1200
Size: 32 131 32 13 32 22 32 450
Cuts at: 1200 1232 1553 1585 1890 1922 2034 2066 2629
Size: 32 321 32 305 32 112 32 563
Cuts at: 2629 2654 2661 2686 3724 3754 3756 3786 456
Size: 25 7 25 1038 30 2 30 2552
Fragments arranged by size:
2552 1038 563 450 321 305 131 112
32 32 32 32 32 32 32 32
30 30 25 25 22 13 7 2
HincII GTy'rAC
Cuts at: 2445 2921 2445
Size: 476 5406
HindIII A'AGCT_T
Cuts at: 892 1996 892
Size: 1104 4778
KasI G'GCGC_C
Cuts at: 2563 3312 3560 2563
Size: 749 248 4885
Fragments arranged by size:
4885 749 248
KpnI G_GTAC'C
Cuts at: 2060 2902 2060
Size: 842 5040
MluI A'CGCG_T
Cuts at: 5315 5315
Size: 5882
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 151 431 617 1730 2296 4553 4737 151
Size: 280 186 1113 566 2257 184 1296
Fragments arranged by size:
2257 1296 1113 566 280 186 184
MscI TGG'CCA
Cuts at: 1557 3643 1557
Size: 2086 3796
MslI CAynn'nnrTG
Cuts at: 3871 3998 3871
Size: 127 5755
MspA1I CmG'CkG
Cuts at: 134 250 325 1660 1816 2021 2103 2813 2993
Size: 116 75 1335 156 205 82 710 180
Cuts at: 2993 3307 3667 4680 4925 134
Size: 314 360 1013 245 1091
Fragments arranged by size:
1335 1091 1013 710 360 314 245 205
180 156 116 82 75
NaeI GCC'GGC
Cuts at: 4063 4063
Size: 5882
NarI GG'CG_CC
Cuts at: 2564 3313 3561 2564
Size: 749 248 4885
Fragments arranged by size:
4885 749 248
NcoI C'CATG_G
Cuts at: 1461 1461
Size: 5882
NgoMIV G'CCGG_C
Cuts at: 4061 4061
Size: 5882
NspI r_CATG'y
Cuts at: 2523 2935 3435 4342 2523
Size: 412 500 907 4063
Fragments arranged by size:
4063 907 500 412
PciI A'CATG_T
Cuts at: 2519 4338 2519
Size: 1819 4063
PflMI CCAn_nnn'nTGG
Cuts at: 1119 1564 1119
Size: 445 5437
PfoI T'CCnGG_A
Cuts at: 195 1520 2249 2633 195
Size: 1325 729 384 3444
Fragments arranged by size:
3444 1325 729 384
PmeI GTTT'AAAC
Cuts at: 2881 2881
Size: 5882
PmlI CAC'GTG
Cuts at: 753 753
Size: 5882
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5021 5053 5021
Size: 32 5850
PshAI GACnn'nnGTC
Cuts at: 2155 2312 2155
Size: 157 5725
PsiI TTA'TAA
Cuts at: 1994 1994
Size: 5882
PspXI vC'TCGA_Gb
Cuts at: 1667 1667
Size: 5882
PstI C_TGCA'G
Cuts at: 215 935 1173 2458 2627 2865 2929 3614 215
Size: 720 238 1285 169 238 64 685 2483
Fragments arranged by size:
2483 1285 720 685 238 238 169 64
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5882
PvuII CAG'CTG
Cuts at: 250 325 1660 2103 2813 3307 3667 250
Size: 75 1335 443 710 494 360 2465
Fragments arranged by size:
2465 1335 710 494 443 360 75
RsrII CG'GwC_CG
Cuts at: 4077 4077
Size: 5882
SacI G_AGCT'C
Cuts at: 433 2896 433
Size: 2463 3419
SacII CC_GC'GG
Cuts at: 135 135
Size: 5882
SalI G'TCGA_C
Cuts at: 2919 2919
Size: 5882
SapI GCTCTTCn'nnn_
Cuts at: 216 3905 4115 216
Size: 3689 210 1983
Fragments arranged by size:
3689 1983 210
SbfI CC_TGCA'GG
Cuts at: 2627 2929 2627
Size: 302 5580
ScaI AGT'ACT
Cuts at: 5399 5399
Size: 5882
SfcI C'TryA_G
Cuts at: 33 211 659 931 1169 1442 1789 2454 2623
Size: 178 448 272 238 273 347 665 169
Cuts at: 2623 2861 2925 3610 4603 4794 33
Size: 238 64 685 993 191 1121
Fragments arranged by size:
1121 993 685 665 448 347 273 272
238 238 191 178 169 64
SfoI GGC'GCC
Cuts at: 2565 3314 3562 2565
Size: 749 248 4885
Fragments arranged by size:
4885 749 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5882
SgrAI Cr'CCGG_yG
Cuts at: 2716 2716
Size: 5882
SmaI CCC'GGG
Cuts at: 2590 2904 2590
Size: 314 5568
SmlI C'TyrA_G
Cuts at: 1667 2025 2611 2646 3037 3952 4444 4706 1667
Size: 358 586 35 391 915 492 262 2843
Fragments arranged by size:
2843 915 586 492 391 358 262 35
SnaBI TAC'GTA
Cuts at: 2874 2874
Size: 5882
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5882
SphI G_CATG'C
Cuts at: 2935 3435 2935
Size: 500 5382
StuI AGG'CCT
Cuts at: 1131 1131
Size: 5882
StyI C'CwwG_G
Cuts at: 802 1160 1461 3016 3370 802
Size: 358 301 1555 354 3314
Fragments arranged by size:
3314 1555 358 354 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2167 3465 3598 4057 4266 2167
Size: 1298 133 459 209 3783
Fragments arranged by size:
3783 1298 459 209 133
TatI w'GTAC_w
Cuts at: 1325 5397 1325
Size: 4072 1810
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 420 452 1397 1429 1559 1591 1932 1964 2310
Size: 32 945 32 130 32 341 32 346
Cuts at: 2310 2342 2747 2779 5399 5431 420
Size: 32 405 32 2620 32 871
Fragments arranged by size:
2620 945 871 405 346 341 130 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1201 3679 1201
Size: 2478 3404
XbaI T'CTAG_A
Cuts at: 2913 2913
Size: 5882
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2205 3377 2205
Size: 1172 4710
XhoI C'TCGA_G
Cuts at: 1667 1667
Size: 5882
XmaI C'CCGG_G
Cuts at: 2588 2902 2588
Size: 314 5568
XmnI GAAnn'nnTTC
Cuts at: 290 290
Size: 5882
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI ApaI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PpuMI PspOMI PsrI SanDI SexAI SfiI SrfI
SspI SwaI ZraI