Restriction map for
pF1KSDA0142
(Circular) (Six-base) MAPSORT of: pF1KSDA0142.seq Check: 9863 from: 1 to: 5077
pF1KSDA0142 5077 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AatII G_ACGT'C
Cuts at: 221 221
Size: 5077
AccI GT'mk_AC
Cuts at: 2113 2113
Size: 5077
Acc65I G'GTAC_C
Cuts at: 2091 2091
Size: 5077
AclI AA'CG_TT
Cuts at: 4917 4917
Size: 5077
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 445 1655 2044 2900 3332 4081 4349 376
Size: 69 1210 389 856 432 749 268 1104
Fragments arranged by size:
1210 1104 856 749 432 389 268 69
AflIII A'CryG_T
Cuts at: 1037 2427 3533 4510 1037
Size: 1390 1106 977 1604
Fragments arranged by size:
1604 1390 1106 977
AgeI A'CCGG_T
Cuts at: 3465 3465
Size: 5077
AleI CACnn'nnGTG
Cuts at: 235 1333 235
Size: 1098 3979
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 319 622 319
Size: 303 4774
AlwNI CAG_nnn'CTG
Cuts at: 975 1050 1287 1731 2380 3949 975
Size: 75 237 444 649 1569 2103
Fragments arranged by size:
2103 1569 649 444 237 75
ApaLI G'TGCA_C
Cuts at: 3847 3847
Size: 5077
ApoI r'AATT_y
Cuts at: 2079 4430 4686 2079
Size: 2351 256 2470
Fragments arranged by size:
2470 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5077
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5077
AvaI C'yCGr_G
Cuts at: 850 2095 850
Size: 1245 3832
AvrII C'CTAG_G
Cuts at: 1135 1135
Size: 5077
BamHI G'GATC_C
Cuts at: 2100 2100
Size: 5077
BanI G'GyrC_C
Cuts at: 760 1480 2091 2505 2753 2788 760
Size: 720 611 414 248 35 3049
Fragments arranged by size:
3049 720 611 414 248 35
BanII G_rGCy'C
Cuts at: 364 851 1621 1989 2089 3119 364
Size: 487 770 368 100 1030 2322
Fragments arranged by size:
2322 1030 770 487 368 100
BbeI G_GCGC'C
Cuts at: 2509 2757 2509
Size: 248 4829
BbvCI CC'TCA_GC
Cuts at: 1581 1581
Size: 5077
BciVI GTATCCnnnnn_n'
Cuts at: 1893 2973 3736 5077 1893
Size: 1080 763 1341 1893
Fragments arranged by size:
1893 1341 1080 763
BclI T'GATC_A
Cuts at: 2595 2595
Size: 5077
BfrBI ATG'CAT
Cuts at: 1225 1912 1225
Size: 687 4390
BglI GCCn_nnn'nGGC
Cuts at: 281 281
Size: 5077
BglII A'GATC_T
Cuts at: 2590 4629 2590
Size: 2039 3038
BlpI GC'TnA_GC
Cuts at: 617 2187 617
Size: 1570 3507
Bme1580I G_kGCm'C
Cuts at: 265 2700 2793 3851 265
Size: 2435 93 1058 1491
Fragments arranged by size:
2435 1491 1058 93
BmgBI CAC'GTC
Cuts at: 1331 1331
Size: 5077
BmrI ACTGGGnnnn_n'
Cuts at: 1005 1512 2287 2368 2703 4849 1005
Size: 507 775 81 335 2146 1233
Fragments arranged by size:
2146 1233 775 507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3442 3442
Size: 5077
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1534 1560 2020 1534
Size: 26 460 4591
Fragments arranged by size:
4591 460 26
Bpu10I CC'TnA_GC
Cuts at: 799 1546 1581 799
Size: 747 35 4295
Fragments arranged by size:
4295 747 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1219 2251 3130 3624 3922 982
Size: 237 1032 879 494 298 2137
Fragments arranged by size:
2137 1032 879 494 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 745 2047 4781 745
Size: 1302 2734 1041
Fragments arranged by size:
2734 1302 1041
BsaAI yAC'GTr
Cuts at: 303 680 2067 2428 303
Size: 377 1387 361 2952
Fragments arranged by size:
2952 1387 377 361
BsaBI GATnn'nnATC
Cuts at: 6 2589 4628 6
Size: 2583 2039 455
Fragments arranged by size:
2583 2039 455
BsaHI Gr'CG_yC
Cuts at: 218 1441 2506 2754 3458 4954 218
Size: 1223 1065 248 704 1496 341
Fragments arranged by size:
1496 1223 1065 704 341 248
BsaWI w'CCGG_w
Cuts at: 944 989 2264 2785 3465 3739 3886 4297 944
Size: 45 1275 521 680 274 147 411 1724
Fragments arranged by size:
1724 1275 680 521 411 274 147 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1672 1702 1672
Size: 30 5047
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 745 1693 745
Size: 948 4129
BseYI C'CCAG_C
Cuts at: 292 2500 3258 3837 4333 292
Size: 2208 758 579 496 1036
Fragments arranged by size:
2208 1036 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1737 1737
Size: 5077
BsiEI CG_ry'CG
Cuts at: 104 276 2617 2663 3873 104
Size: 172 2341 46 1210 1308
Fragments arranged by size:
2341 1308 1210 172 46
BsiHKAI G_wGCw'C
Cuts at: 851 1307 2089 2867 3057 3851 851
Size: 456 782 778 190 794 2077
Fragments arranged by size:
2077 794 782 778 456 190
BsmI GAATG_Cn'
Cuts at: 601 601
Size: 5077
BsmBI CGTCTCn'nnnn_
Cuts at: 209 209
Size: 5077
Bsp1286I G_dGCh'C
Cuts at: 265 364 851 1307 1621 1989 2089 2700 2793
Size: 99 487 456 314 368 100 611 93
Cuts at: 2793 2867 3057 3119 3851 265
Size: 74 190 62 732 1491
Fragments arranged by size:
1491 732 611 487 456 368 314 190
100 99 93 74 62
BspEI T'CCGG_A
Cuts at: 944 989 4297 944
Size: 45 3308 1724
Fragments arranged by size:
3308 1724 45
BspHI T'CATG_A
Cuts at: 4253 4253
Size: 5077
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2125 2339 2641 3022 2125
Size: 214 302 381 4180
Fragments arranged by size:
4180 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1254 3367 3421 4907 5072 50
Size: 1204 2113 54 1486 165 55
Fragments arranged by size:
2113 1486 1204 165 55 54
BsrDI GCAATG_nn'
Cuts at: 490 2987 490
Size: 2497 2580
BsrFI r'CCGG_y
Cuts at: 280 3073 3254 3465 280
Size: 2793 181 211 1892
Fragments arranged by size:
2793 1892 211 181
BssSI C'ACGA_G
Cuts at: 781 3346 3706 781
Size: 2565 360 2152
Fragments arranged by size:
2565 2152 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1287 2452 1287
Size: 1165 3912
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5077
BstYI r'GATC_y
Cuts at: 1 971 1284 1605 2100 2567 2590 2925 3171
Size: 970 313 321 495 467 23 335 246
Cuts at: 3171 4185 4271 4283 4629 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 970 495 467 449 346 335 321
313 246 86 23 12
BtgI C'CryG_G
Cuts at: 791 902 1476 791
Size: 111 574 4392
Fragments arranged by size:
4392 574 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1019 3025 3206 3369 1019
Size: 2006 181 163 2727
Fragments arranged by size:
2727 2006 181 163
BtsI GCAGTG_nn'
Cuts at: 710 2318 710
Size: 1608 3469
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1799 1834 1799
Size: 35 5042
DraI TTT'AAA
Cuts at: 644 2074 644
Size: 1430 3647
DrdI GACnn_nn'nnGTC
Cuts at: 2781 3641 2781
Size: 860 4217
EaeI y'GGCC_r
Cuts at: 273 487 2660 2834 3225 3252 4599 273
Size: 214 2173 174 391 27 1347 751
Fragments arranged by size:
2173 1347 751 391 214 174 27
EagI C'GGCC_G
Cuts at: 273 2660 273
Size: 2387 2690
EarI CTCTTCn'nnn_
Cuts at: 236 352 943 1105 1826 3098 3308 236
Size: 116 591 162 721 1272 210 2005
Fragments arranged by size:
2005 1272 721 591 210 162 116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3283 3595 3741 4887 3283
Size: 312 146 1146 3473
Fragments arranged by size:
3473 1146 312 146
EcoICRI GAG'CTC
Cuts at: 849 2087 849
Size: 1238 3839
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 445 1534 1560 1655 2020 2044 2900 3332
Size: 69 1089 26 95 365 24 856 432
Cuts at: 3332 4081 4349 376
Size: 749 268 1104
Fragments arranged by size:
1104 1089 856 749 432 365 268 95
69 26 24
EcoNI CCTnn'n_nnAGG
Cuts at: 368 368
Size: 5077
EcoO109I rG'GnC_Cy
Cuts at: 1047 1507 2214 1047
Size: 460 707 3910
Fragments arranged by size:
3910 707 460
EcoRI G'AATT_C
Cuts at: 2079 2079
Size: 5077
EcoRV GAT'ATC
Cuts at: 1090 1090
Size: 5077
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 189 1837 3094 189
Size: 1648 1257 2172
Fragments arranged by size:
2172 1648 1257
FspI TGC'GCA
Cuts at: 2856 2856
Size: 5077
HaeII r_GCGC'y
Cuts at: 1763 2509 2757 3781 4760 1763
Size: 746 248 1024 979 2080
Fragments arranged by size:
2080 1024 979 746 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 206 238 1672 1704 2917 2947 2949 2979 206
Size: 32 1434 32 1213 30 2 30 2304
Fragments arranged by size:
2304 1434 1213 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2114 2114
Size: 5077
HindIII A'AGCT_T
Cuts at: 1165 1195 1745 1165
Size: 30 550 4497
Fragments arranged by size:
4497 550 30
KasI G'GCGC_C
Cuts at: 2505 2753 2505
Size: 248 4829
KpnI G_GTAC'C
Cuts at: 2095 2095
Size: 5077
MluI A'CGCG_T
Cuts at: 4510 4510
Size: 5077
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 609 1195 3748 3932 609
Size: 586 2553 184 1754
Fragments arranged by size:
2553 1754 586 184
MscI TGG'CCA
Cuts at: 2836 2836
Size: 5077
MslI CAynn'nnrTG
Cuts at: 235 1036 1220 1333 3064 3191 235
Size: 801 184 113 1731 127 2121
Fragments arranged by size:
2121 1731 801 184 127 113
MspA1I CmG'CkG
Cuts at: 1437 1585 1705 2186 2500 2860 3875 4120 1437
Size: 148 120 481 314 360 1015 245 2394
Fragments arranged by size:
2394 1015 481 360 314 245 148 120
NaeI GCC'GGC
Cuts at: 3256 3256
Size: 5077
NarI GG'CG_CC
Cuts at: 2506 2754 2506
Size: 248 4829
NcoI C'CATG_G
Cuts at: 902 902
Size: 5077
NgoMIV G'CCGG_C
Cuts at: 3254 3254
Size: 5077
NruI TCG'CGA
Cuts at: 3438 3438
Size: 5077
NsiI A_TGCA'T
Cuts at: 1227 1914 1227
Size: 687 4390
NspI r_CATG'y
Cuts at: 1041 2128 2628 3537 1041
Size: 1087 500 909 2581
Fragments arranged by size:
2581 1087 909 500
PasI CC'CwG_GG
Cuts at: 772 772
Size: 5077
PciI A'CATG_T
Cuts at: 1037 3533 1037
Size: 2496 2581
PflMI CCAn_nnn'nTGG
Cuts at: 1502 1538 1502
Size: 36 5041
PfoI T'CCnGG_A
Cuts at: 1690 1690
Size: 5077
PmeI GTTT'AAAC
Cuts at: 2074 2074
Size: 5077
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4216 4248 4216
Size: 32 5045
PpuMI rG'GwC_Cy
Cuts at: 1047 1507 1047
Size: 460 4617
PstI C_TGCA'G
Cuts at: 705 971 1292 2122 2807 705
Size: 266 321 830 685 2975
Fragments arranged by size:
2975 830 685 321 266
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5077
PvuII CAG'CTG
Cuts at: 1585 1705 2500 2860 1585
Size: 120 795 360 3802
Fragments arranged by size:
3802 795 360 120
RsrII CG'GwC_CG
Cuts at: 278 3270 278
Size: 2992 2085
SacI G_AGCT'C
Cuts at: 851 2089 851
Size: 1238 3839
SalI G'TCGA_C
Cuts at: 2112 2112
Size: 5077
SanDI GG'GwC_CC
Cuts at: 1507 1507
Size: 5077
SapI GCTCTTCn'nnn_
Cuts at: 352 3098 3308 352
Size: 2746 210 2121
Fragments arranged by size:
2746 2121 210
SbfI CC_TGCA'GG
Cuts at: 2122 2122
Size: 5077
ScaI AGT'ACT
Cuts at: 4594 4594
Size: 5077
SfcI C'TryA_G
Cuts at: 33 418 701 967 1288 1309 2118 2803 3798
Size: 385 283 266 321 21 809 685 995
Cuts at: 3798 3989 33
Size: 191 1121
Fragments arranged by size:
1121 995 809 685 385 321 283 266
191 21
SfoI GGC'GCC
Cuts at: 2507 2755 2507
Size: 248 4829
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5077
SmaI CCC'GGG
Cuts at: 2097 2097
Size: 5077
SmlI C'TyrA_G
Cuts at: 850 961 1198 2230 3145 3639 3901 850
Size: 111 237 1032 915 494 262 2026
Fragments arranged by size:
2026 1032 915 494 262 237 111
SnaBI TAC'GTA
Cuts at: 2067 2067
Size: 5077
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5077
SphI G_CATG'C
Cuts at: 2128 2628 2128
Size: 500 4577
SspI AAT'ATT
Cuts at: 426 448 426
Size: 22 5055
StyI C'CwwG_G
Cuts at: 902 1135 2209 2563 902
Size: 233 1074 354 3416
Fragments arranged by size:
3416 1074 354 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 145 1287 1545 2658 2791 3250 3461 145
Size: 1142 258 1113 133 459 211 1761
Fragments arranged by size:
1761 1142 1113 459 258 211 133
TatI w'GTAC_w
Cuts at: 4592 4592
Size: 5077
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4594 4626 4594
Size: 32 5045
Tth111I GACn'n_nGTC
Cuts at: 2872 2872
Size: 5077
XbaI T'CTAG_A
Cuts at: 546 1968 2106 546
Size: 1422 138 3517
Fragments arranged by size:
3517 1422 138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2570 2570
Size: 5077
XhoI C'TCGA_G
Cuts at: 850 850
Size: 5077
XmaI C'CCGG_G
Cuts at: 2095 2095
Size: 5077
ZraI GAC'GTC
Cuts at: 219 219
Size: 5077
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI ApaI AscI BaeI
BbsI BcgI BmtI BsiWI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII FseI FspAI HpaI MfeI
NdeI NheI NotI PacI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SrfI StuI
SwaI XmnI