Restriction map for
pF1KSDA0140
(Circular) (Six-base) MAPSORT of: pF1KSDA0140.seq Check: 4534 from: 1 to: 4403
pF1KSDA0140 4403 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 736 882 736
Size: 146 4257
AccI GT'mk_AC
Cuts at: 1441 1441
Size: 4403
Acc65I G'GTAC_C
Cuts at: 1419 1419
Size: 4403
AclI AA'CG_TT
Cuts at: 4243 4243
Size: 4403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 283 585 612 1001 1029 2228 2660 3407
Size: 109 302 27 389 28 1199 432 747
Cuts at: 3407 3675 174
Size: 268 902
Fragments arranged by size:
1199 902 747 432 389 302 268 109
28 27
AfeI AGC'GCT
Cuts at: 586 586
Size: 4403
AflIII A'CryG_T
Cuts at: 1755 2859 3836 1755
Size: 1104 977 2322
Fragments arranged by size:
2322 1104 977
AgeI A'CCGG_T
Cuts at: 2791 2791
Size: 4403
AlwNI CAG_nnn'CTG
Cuts at: 326 1130 1346 1708 3275 326
Size: 804 216 362 1567 1454
Fragments arranged by size:
1567 1454 804 362 216
ApaI G_GGCC'C
Cuts at: 1332 1332
Size: 4403
ApaLI G'TGCA_C
Cuts at: 3173 3173
Size: 4403
ApoI r'AATT_y
Cuts at: 1407 3756 4012 1407
Size: 2349 256 1798
Fragments arranged by size:
2349 1798 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4403
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
AvaI C'yCGr_G
Cuts at: 158 641 1423 158
Size: 483 782 3138
Fragments arranged by size:
3138 782 483
BamHI G'GATC_C
Cuts at: 1428 1428
Size: 4403
BanI G'GyrC_C
Cuts at: 362 717 974 1419 1833 2081 2116 362
Size: 355 257 445 414 248 35 2649
Fragments arranged by size:
2649 445 414 355 257 248 35
BanII G_rGCy'C
Cuts at: 529 763 1099 1332 1417 2447 529
Size: 234 336 233 85 1030 2485
Fragments arranged by size:
2485 1030 336 234 233 85
BbeI G_GCGC'C
Cuts at: 978 1837 2085 978
Size: 859 248 3296
Fragments arranged by size:
3296 859 248
BbvCI CC'TCA_GC
Cuts at: 151 412 441 858 1054 151
Size: 261 29 417 196 3500
Fragments arranged by size:
3500 417 261 196 29
BciVI GTATCCnnnnn_n'
Cuts at: 2301 3062 4403 2301
Size: 761 1341 2301
Fragments arranged by size:
2301 1341 761
BclI T'GATC_A
Cuts at: 401 1923 401
Size: 1522 2881
BglI GCCn_nnn'nGGC
Cuts at: 1024 1163 1024
Size: 139 4264
BglII A'GATC_T
Cuts at: 1918 3955 1918
Size: 2037 2366
BlpI GC'TnA_GC
Cuts at: 726 1515 726
Size: 789 3614
Bme1580I G_kGCm'C
Cuts at: 1332 2028 2121 3177 1332
Size: 696 93 1056 2558
Fragments arranged by size:
2558 1056 696 93
BmgBI CAC'GTC
Cuts at: 1113 1113
Size: 4403
BmrI ACTGGGnnnn_n'
Cuts at: 1615 1696 2031 4175 1615
Size: 81 335 2144 1843
Fragments arranged by size:
2144 1843 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1022 2770 1022
Size: 1748 2655
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 777 888 777
Size: 111 4292
Bpu10I CC'TnA_GC
Cuts at: 151 412 441 858 1054 1063 1193 151
Size: 261 29 417 196 9 130 3361
Fragments arranged by size:
3361 417 261 196 130 29 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1579 2458 2950 3248 1579
Size: 879 492 298 2734
Fragments arranged by size:
2734 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 269 4107 269
Size: 3838 565
BsaAI yAC'GTr
Cuts at: 1395 1756 1395
Size: 361 4042
BsaBI GATnn'nnATC
Cuts at: 6 1917 3954 6
Size: 1911 2037 455
Fragments arranged by size:
2037 1911 455
BsaHI Gr'CG_yC
Cuts at: 975 1834 2082 2784 4280 975
Size: 859 248 702 1496 1098
Fragments arranged by size:
1496 1098 859 702 248
BsaWI w'CCGG_w
Cuts at: 1592 2113 2791 3065 3212 3623 1592
Size: 521 678 274 147 411 2372
Fragments arranged by size:
2372 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 640 670 1228 1258 640
Size: 30 558 30 3785
Fragments arranged by size:
3785 558 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 630 648 797 1267 630
Size: 18 149 470 3766
Fragments arranged by size:
3766 470 149 18
BseYI C'CCAG_C
Cuts at: 929 1177 1828 2586 3163 3659 929
Size: 248 651 758 577 496 1673
Fragments arranged by size:
1673 758 651 577 496 248
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 754 187
Size: 567 3836
BsiEI CG_ry'CG
Cuts at: 104 1945 1991 3199 104
Size: 1841 46 1208 1308
Fragments arranged by size:
1841 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 319 656 1417 2195 2385 3177 319
Size: 337 761 778 190 792 1545
Fragments arranged by size:
1545 792 778 761 337 190
BsmI GAATG_Cn'
Cuts at: 337 337
Size: 4403
BsmBI CGTCTCn'nnnn_
Cuts at: 549 891 549
Size: 342 4061
Bsp1286I G_dGCh'C
Cuts at: 319 529 656 763 1099 1332 1417 2028 2121
Size: 210 127 107 336 233 85 611 93
Cuts at: 2121 2195 2385 2447 3177 319
Size: 74 190 62 730 1545
Fragments arranged by size:
1545 730 611 336 233 210 190 127
107 93 85 74 62
BspEI T'CCGG_A
Cuts at: 3623 3623
Size: 4403
BspHI T'CATG_A
Cuts at: 3579 3579
Size: 4403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 736 776 806 882 1453 1667 1969 2350 736
Size: 40 30 76 571 214 302 381 2789
Fragments arranged by size:
2789 571 381 302 214 76 40 30
BsrBI CCG'CTC
Cuts at: 50 470 926 1297 2695 2749 4233 4398 50
Size: 420 456 371 1398 54 1484 165 55
Fragments arranged by size:
1484 1398 456 420 371 165 55 54
BsrDI GCAATG_nn'
Cuts at: 173 2315 173
Size: 2142 2261
BsrFI r'CCGG_y
Cuts at: 788 1302 2401 2582 2791 788
Size: 514 1099 181 209 2400
Fragments arranged by size:
2400 1099 514 209 181
BssSI C'ACGA_G
Cuts at: 2674 3032 2674
Size: 358 4045
BstAPI GCAn_nnn'nTGC
Cuts at: 1780 1780
Size: 4403
BstBI TT'CG_AA
Cuts at: 110 2764 110
Size: 2654 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 132 694 132
Size: 562 3841
BstYI r'GATC_y
Cuts at: 1 1428 1895 1918 2253 2499 3511 3597 3609
Size: 1427 467 23 335 246 1012 86 12
Cuts at: 3609 3955 1
Size: 346 449
Fragments arranged by size:
1427 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 545 776 1323 125
Size: 420 231 547 3205
Fragments arranged by size:
3205 547 420 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2353 2534 2697 2353
Size: 181 163 4059
Fragments arranged by size:
4059 181 163
BtsI GCAGTG_nn'
Cuts at: 380 1646 380
Size: 1266 3137
DraI TTT'AAA
Cuts at: 1402 1402
Size: 4403
DraIII CAC_nnn'GTG
Cuts at: 777 777
Size: 4403
DrdI GACnn_nn'nnGTC
Cuts at: 955 1225 2109 2967 955
Size: 270 884 858 2391
Fragments arranged by size:
2391 884 858 270
EaeI y'GGCC_r
Cuts at: 1988 2162 2553 2580 3925 1988
Size: 174 391 27 1345 2466
Fragments arranged by size:
2466 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1988 1988
Size: 4403
EarI CTCTTCn'nnn_
Cuts at: 302 1205 2426 2636 302
Size: 903 1221 210 2069
Fragments arranged by size:
2069 1221 903 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2611 2921 3067 4213 2611
Size: 310 146 1146 2801
Fragments arranged by size:
2801 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1415 1415
Size: 4403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 283 585 612 777 888 1001 1029 2228
Size: 109 302 27 165 111 113 28 1199
Cuts at: 2228 2660 3407 3675 174
Size: 432 747 268 902
Fragments arranged by size:
1199 902 747 432 302 268 165 113
111 109 28 27
EcoNI CCTnn'n_nnAGG
Cuts at: 1276 1276
Size: 4403
EcoO109I rG'GnC_Cy
Cuts at: 185 229 518 1127 1169 1181 1239 1328 1329
Size: 44 289 609 42 12 58 89 1
Cuts at: 1329 1542 185
Size: 213 3046
Fragments arranged by size:
3046 609 289 213 89 58 44 42
12 1
EcoRI G'AATT_C
Cuts at: 1407 1407
Size: 4403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4403
FspI TGC'GCA
Cuts at: 2184 2184
Size: 4403
HaeII r_GCGC'y
Cuts at: 462 588 978 1837 2085 3107 4086 462
Size: 126 390 859 248 1022 979 779
Fragments arranged by size:
1022 979 859 779 390 248 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 510 1228 1260 2245 2275 2277 2307 478
Size: 32 718 32 985 30 2 30 2574
Fragments arranged by size:
2574 985 718 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1442 1442
Size: 4403
KasI G'GCGC_C
Cuts at: 974 1833 2081 974
Size: 859 248 3296
Fragments arranged by size:
3296 859 248
KpnI G_GTAC'C
Cuts at: 1423 1423
Size: 4403
MluI A'CGCG_T
Cuts at: 3836 3836
Size: 4403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 617 1346 3074 3258 617
Size: 729 1728 184 1762
Fragments arranged by size:
1762 1728 729 184
MscI TGG'CCA
Cuts at: 2164 2164
Size: 4403
MslI CAynn'nnrTG
Cuts at: 130 2392 2519 130
Size: 2262 127 2014
Fragments arranged by size:
2262 2014 127
MspA1I CmG'CkG
Cuts at: 569 804 908 1058 1177 1265 1325 1514 1828
Size: 235 104 150 119 88 60 189 314
Cuts at: 1828 2188 3201 3446 569
Size: 360 1013 245 1526
Fragments arranged by size:
1526 1013 360 314 245 235 189 150
119 104 88 60
NaeI GCC'GGC
Cuts at: 790 1304 2584 790
Size: 514 1280 2609
Fragments arranged by size:
2609 1280 514
NarI GG'CG_CC
Cuts at: 975 1834 2082 975
Size: 859 248 3296
Fragments arranged by size:
3296 859 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4403
NgoMIV G'CCGG_C
Cuts at: 788 1302 2582 788
Size: 514 1280 2609
Fragments arranged by size:
2609 1280 514
NspI r_CATG'y
Cuts at: 182 1456 1956 2863 182
Size: 1274 500 907 1722
Fragments arranged by size:
1722 1274 907 500
PasI CC'CwG_GG
Cuts at: 1159 1333 1159
Size: 174 4229
PciI A'CATG_T
Cuts at: 2859 2859
Size: 4403
PflMI CCAn_nnn'nTGG
Cuts at: 326 536 1130 326
Size: 210 594 3599
Fragments arranged by size:
3599 594 210
PmeI GTTT'AAAC
Cuts at: 1402 1402
Size: 4403
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 3542
Size: 32 4371
PpuMI rG'GwC_Cy
Cuts at: 185 229 1169 1181 1239 185
Size: 44 940 12 58 3349
Fragments arranged by size:
3349 940 58 44 12
PshAI GACnn'nnGTC
Cuts at: 1085 1186 1085
Size: 101 4302
PspOMI G'GGCC_C
Cuts at: 1328 1328
Size: 4403
PstI C_TGCA'G
Cuts at: 803 1311 1450 2135 803
Size: 508 139 685 3071
Fragments arranged by size:
3071 685 508 139
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4403
PvuII CAG'CTG
Cuts at: 1058 1265 1828 2188 1058
Size: 207 563 360 3273
Fragments arranged by size:
3273 563 360 207
RsrII CG'GwC_CG
Cuts at: 2598 2598
Size: 4403
SacI G_AGCT'C
Cuts at: 1417 1417
Size: 4403
SacII CC_GC'GG
Cuts at: 1326 1326
Size: 4403
SalI G'TCGA_C
Cuts at: 1440 1440
Size: 4403
SanDI GG'GwC_CC
Cuts at: 1181 1181
Size: 4403
SapI GCTCTTCn'nnn_
Cuts at: 2426 2636 2426
Size: 210 4193
SbfI CC_TGCA'GG
Cuts at: 1450 1450
Size: 4403
ScaI AGT'ACT
Cuts at: 3920 3920
Size: 4403
SfcI C'TryA_G
Cuts at: 33 564 799 1307 1446 2131 3124 3315 33
Size: 531 235 508 139 685 993 191 1121
Fragments arranged by size:
1121 993 685 531 508 235 191 139
SfoI GGC'GCC
Cuts at: 976 1835 2083 976
Size: 859 248 3296
Fragments arranged by size:
3296 859 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
SmaI CCC'GGG
Cuts at: 160 643 1425 160
Size: 483 782 3138
Fragments arranged by size:
3138 782 483
SmlI C'TyrA_G
Cuts at: 1558 2473 2965 3227 1558
Size: 915 492 262 2734
Fragments arranged by size:
2734 915 492 262
SnaBI TAC'GTA
Cuts at: 1395 1395
Size: 4403
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4403
SphI G_CATG'C
Cuts at: 1456 1956 1456
Size: 500 3903
StuI AGG'CCT
Cuts at: 1002 1002
Size: 4403
StyI C'CwwG_G
Cuts at: 125 521 711 1537 1891 125
Size: 396 190 826 354 2637
Fragments arranged by size:
2637 826 396 354 190
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1986 2119 2578 2787 1986
Size: 133 459 209 3602
Fragments arranged by size:
3602 459 209 133
TatI w'GTAC_w
Cuts at: 3918 3918
Size: 4403
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3920 3952 3920
Size: 32 4371
Tth111I GACn'n_nGTC
Cuts at: 2200 2200
Size: 4403
XbaI T'CTAG_A
Cuts at: 1008 1434 1008
Size: 426 3977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 691 1898 691
Size: 1207 3196
XmaI C'CCGG_G
Cuts at: 158 641 1423 158
Size: 483 782 3138
Fragments arranged by size:
3138 782 483
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmtI BsiWI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PsiI PspXI PsrI SexAI SfiI
SgrAI SrfI SspI SwaI XhoI XmnI ZraI