Restriction map for
pF1KSDA0132
(Circular) (Six-base) MAPSORT of: pF1KSDA0132.seq Check: 8626 from: 1 to: 5009
pF1KSDA0132 5009 bp
With 182 enzymes: SgfI *
February 20, 2009 16:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1059 1191 1059
Size: 132 4877
AatII G_ACGT'C
Cuts at: 362 362
Size: 5009
AccI GT'mk_AC
Cuts at: 2047 2047
Size: 5009
Acc65I G'GTAC_C
Cuts at: 2025 2025
Size: 5009
AclI AA'CG_TT
Cuts at: 4849 4849
Size: 5009
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 2834 3266 4013 4281 264
Size: 2570 432 747 268 992
Fragments arranged by size:
2570 992 747 432 268
AfeI AGC'GCT
Cuts at: 1732 1732
Size: 5009
AflIII A'CryG_T
Cuts at: 1850 2361 3465 4442 1850
Size: 511 1104 977 2417
Fragments arranged by size:
2417 1104 977 511
AgeI A'CCGG_T
Cuts at: 3397 3397
Size: 5009
AleI CACnn'nnGTG
Cuts at: 728 1069 1439 728
Size: 341 370 4298
Fragments arranged by size:
4298 370 341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 746 1677 746
Size: 931 4078
AlwNI CAG_nnn'CTG
Cuts at: 351 660 924 2314 3881 351
Size: 309 264 1390 1567 1479
Fragments arranged by size:
1567 1479 1390 309 264
ApaLI G'TGCA_C
Cuts at: 3779 3779
Size: 5009
ApoI r'AATT_y
Cuts at: 538 2013 4362 4618 538
Size: 1475 2349 256 929
Fragments arranged by size:
2349 1475 929 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5009
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5009
AvaI C'yCGr_G
Cuts at: 734 2029 734
Size: 1295 3714
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1662 1695 1662
Size: 33 4976
BamHI G'GATC_C
Cuts at: 2034 2034
Size: 5009
BanI G'GyrC_C
Cuts at: 1086 1174 1203 2025 2439 2687 2722 1086
Size: 88 29 822 414 248 35 3373
Fragments arranged by size:
3373 822 414 248 88 35 29
BanII G_rGCy'C
Cuts at: 1049 1961 2023 3053 1049
Size: 912 62 1030 3005
Fragments arranged by size:
3005 1030 912 62
BbeI G_GCGC'C
Cuts at: 1090 2443 2691 1090
Size: 1353 248 3408
Fragments arranged by size:
3408 1353 248
BbsI GAAGACnn'nnnn_
Cuts at: 435 852 435
Size: 417 4592
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 660 694 948 982 660
Size: 34 254 34 4687
Fragments arranged by size:
4687 254 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 517 2907 3668 5009 517
Size: 2390 761 1341 517
Fragments arranged by size:
2390 1341 761 517
BclI T'GATC_A
Cuts at: 1622 2529 1622
Size: 907 4102
BfrBI ATG'CAT
Cuts at: 753 753
Size: 5009
BglI GCCn_nnn'nGGC
Cuts at: 550 1223 550
Size: 673 4336
BglII A'GATC_T
Cuts at: 1034 2524 4561 1034
Size: 1490 2037 1482
Fragments arranged by size:
2037 1490 1482
BlpI GC'TnA_GC
Cuts at: 340 698 1141 2121 340
Size: 358 443 980 3228
Fragments arranged by size:
3228 980 443 358
Bme1580I G_kGCm'C
Cuts at: 196 735 1361 1794 2634 2727 3783 196
Size: 539 626 433 840 93 1056 1422
Fragments arranged by size:
1422 1056 840 626 539 433 93
BmgBI CAC'GTC
Cuts at: 588 1754 588
Size: 1166 3843
BmrI ACTGGGnnnn_n'
Cuts at: 393 888 1160 2221 2302 2637 4781 393
Size: 495 272 1061 81 335 2144 621
Fragments arranged by size:
2144 1061 621 495 335 272 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3376 3376
Size: 5009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 1171 1278 1917 313
Size: 858 107 639 3405
Fragments arranged by size:
3405 858 639 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2185 3064 3556 3854 2185
Size: 879 492 298 3340
Fragments arranged by size:
3340 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4713 4713
Size: 5009
BsaAI yAC'GTr
Cuts at: 2001 2362 2001
Size: 361 4648
BsaBI GATnn'nnATC
Cuts at: 6 2523 4560 6
Size: 2517 2037 455
Fragments arranged by size:
2517 2037 455
BsaHI Gr'CG_yC
Cuts at: 253 359 955 1087 1658 2440 2688 3390 4886
Size: 106 596 132 571 782 248 702 1496
Cuts at: 4886 253
Size: 376
Fragments arranged by size:
1496 782 702 596 571 376 248 132
106
BsaWI w'CCGG_w
Cuts at: 1184 2198 2719 3397 3671 3818 4229 1184
Size: 1014 521 678 274 147 411 1964
Fragments arranged by size:
1964 1014 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 478 508 538 568 1276 1306 478
Size: 30 30 30 708 30 4181
Fragments arranged by size:
4181 708 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 793 1057 793
Size: 264 4745
BseYI C'CCAG_C
Cuts at: 154 260 667 1690 2434 3192 3769 4265 154
Size: 106 407 1023 744 758 577 496 898
Fragments arranged by size:
1023 898 758 744 577 496 407 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 1039 1654 673
Size: 366 615 4028
Fragments arranged by size:
4028 615 366
BsiEI CG_ry'CG
Cuts at: 104 395 2551 2597 3805 104
Size: 291 2156 46 1208 1308
Fragments arranged by size:
2156 1308 1208 291 46
BsiHKAI G_wGCw'C
Cuts at: 1049 2023 2801 2991 3783 1049
Size: 974 778 190 792 2275
Fragments arranged by size:
2275 974 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1744 1744
Size: 5009
Bsp1286I G_dGCh'C
Cuts at: 196 735 1049 1361 1794 1961 2023 2634 2727
Size: 539 314 312 433 167 62 611 93
Cuts at: 2727 2801 2991 3053 3783 196
Size: 74 190 62 730 1422
Fragments arranged by size:
1422 730 611 539 433 314 312 190
167 93 74 62 62
BspEI T'CCGG_A
Cuts at: 4229 4229
Size: 5009
BspHI T'CATG_A
Cuts at: 323 590 4185 323
Size: 267 3595 1147
Fragments arranged by size:
3595 1147 267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 360 1059 1191 1205 2059 2273 2575 2956 360
Size: 699 132 14 854 214 302 381 2413
Fragments arranged by size:
2413 854 699 381 302 214 132 14
BsrBI CCG'CTC
Cuts at: 50 1214 1464 3301 3355 4839 5004 50
Size: 1164 250 1837 54 1484 165 55
Fragments arranged by size:
1837 1484 1164 250 165 55 54
BsrDI GCAATG_nn'
Cuts at: 678 1637 2921 678
Size: 959 1284 2766
Fragments arranged by size:
2766 1284 959
BsrFI r'CCGG_y
Cuts at: 390 1222 1781 3007 3188 3397 390
Size: 832 559 1226 181 209 2002
Fragments arranged by size:
2002 1226 832 559 209 181
BssSI C'ACGA_G
Cuts at: 3280 3638 3280
Size: 358 4651
BstAPI GCAn_nnn'nTGC
Cuts at: 1074 2386 1074
Size: 1312 3697
BstBI TT'CG_AA
Cuts at: 110 3370 110
Size: 3260 1749
BstEII G'GTnAC_C
Cuts at: 810 1905 810
Size: 1095 3914
BstXI CCAn_nnnn'nTGG
Cuts at: 522 564 1302 522
Size: 42 738 4229
Fragments arranged by size:
4229 738 42
BstYI r'GATC_y
Cuts at: 1 135 992 1034 2034 2501 2524 2859 3105
Size: 134 857 42 1000 467 23 335 246
Cuts at: 3105 4117 4203 4215 4561 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 1000 857 467 449 346 335 246
134 86 42 23 12
Bsu36I CC'TnA_GG
Cuts at: 197 197
Size: 5009
BtgI C'CryG_G
Cuts at: 563 1115 1207 1253 1419 1547 1952 563
Size: 552 92 46 166 128 405 3620
Fragments arranged by size:
3620 552 405 166 128 92 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 669 2959 3140 3303 669
Size: 2290 181 163 2375
Fragments arranged by size:
2375 2290 181 163
BtsI GCAGTG_nn'
Cuts at: 364 645 798 2252 364
Size: 281 153 1454 3121
Fragments arranged by size:
3121 1454 281 153
ClaI AT'CG_AT
Cuts at: 1388 1388
Size: 5009
DraI TTT'AAA
Cuts at: 2008 2008
Size: 5009
DraIII CAC_nnn'GTG
Cuts at: 236 417 1482 236
Size: 181 1065 3763
Fragments arranged by size:
3763 1065 181
DrdI GACnn_nn'nnGTC
Cuts at: 625 1027 2715 3573 625
Size: 402 1688 858 2061
Fragments arranged by size:
2061 1688 858 402
EaeI y'GGCC_r
Cuts at: 392 767 1220 1392 2594 2768 3159 3186 4531
Size: 375 453 172 1202 174 391 27 1345
Cuts at: 4531 392
Size: 870
Fragments arranged by size:
1345 1202 870 453 391 375 174 172
27
EagI C'GGCC_G
Cuts at: 392 2594 392
Size: 2202 2807
EarI CTCTTCn'nnn_
Cuts at: 3032 3242 3032
Size: 210 4799
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 259 1207 3217 3527 3673 4819 259
Size: 948 2010 310 146 1146 449
Fragments arranged by size:
2010 1146 948 449 310 146
EcoICRI GAG'CTC
Cuts at: 1047 2021 1047
Size: 974 4035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 264 313 1171 1278 1917 2834 3266 4013 4281
Size: 49 858 107 639 917 432 747 268
Cuts at: 4281 264
Size: 992
Fragments arranged by size:
992 917 858 747 639 432 268 107
49
EcoO109I rG'GnC_Cy
Cuts at: 158 2148 158
Size: 1990 3019
EcoRI G'AATT_C
Cuts at: 538 2013 538
Size: 1475 3534
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 330 3028 330
Size: 2698 2311
FspI TGC'GCA
Cuts at: 2790 2790
Size: 5009
HaeII r_GCGC'y
Cuts at: 531 602 925 1090 1301 1734 2443 2691 3713
Size: 71 323 165 211 433 709 248 1022
Cuts at: 3713 4692 531
Size: 979 848
Fragments arranged by size:
1022 979 848 709 433 323 248 211
165 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 510 1124 1156 2851 2881 2883 2913 478
Size: 32 614 32 1695 30 2 30 2574
Fragments arranged by size:
2574 1695 614 32 32 30 30 2
HincII GTy'rAC
Cuts at: 953 2048 953
Size: 1095 3914
KasI G'GCGC_C
Cuts at: 1086 2439 2687 1086
Size: 1353 248 3408
Fragments arranged by size:
3408 1353 248
KpnI G_GTAC'C
Cuts at: 2029 2029
Size: 5009
MluI A'CGCG_T
Cuts at: 4442 4442
Size: 5009
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1028 1155 3680 3864 1028
Size: 127 2525 184 2173
Fragments arranged by size:
2525 2173 184 127
MscI TGG'CCA
Cuts at: 769 1394 2770 769
Size: 625 1376 3008
Fragments arranged by size:
3008 1376 625
MslI CAynn'nnrTG
Cuts at: 487 562 728 748 1069 1354 1375 1400 1439
Size: 75 166 20 321 285 21 25 39
Cuts at: 1439 1630 2998 3125 487
Size: 191 1368 127 2371
Fragments arranged by size:
2371 1368 321 285 191 166 127 75
39 25 21 20
MspA1I CmG'CkG
Cuts at: 351 660 978 1013 1117 1209 1585 1716 2120
Size: 309 318 35 104 92 376 131 404
Cuts at: 2120 2434 2794 3807 4052 351
Size: 314 360 1013 245 1308
Fragments arranged by size:
1308 1013 404 376 360 318 314 309
245 131 104 92 35
NaeI GCC'GGC
Cuts at: 1224 3190 1224
Size: 1966 3043
NarI GG'CG_CC
Cuts at: 1087 2440 2688 1087
Size: 1353 248 3408
Fragments arranged by size:
3408 1353 248
NcoI C'CATG_G
Cuts at: 563 1952 563
Size: 1389 3620
NgoMIV G'CCGG_C
Cuts at: 1222 3188 1222
Size: 1966 3043
NsiI A_TGCA'T
Cuts at: 755 755
Size: 5009
NspI r_CATG'y
Cuts at: 753 2062 2562 3469 753
Size: 1309 500 907 2293
Fragments arranged by size:
2293 1309 907 500
PasI CC'CwG_GG
Cuts at: 1794 1794
Size: 5009
PciI A'CATG_T
Cuts at: 3465 3465
Size: 5009
PflMI CCAn_nnn'nTGG
Cuts at: 405 417 1331 405
Size: 12 914 4083
Fragments arranged by size:
4083 914 12
PfoI T'CCnGG_A
Cuts at: 1856 1856
Size: 5009
PmeI GTTT'AAAC
Cuts at: 2008 2008
Size: 5009
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 4148
Size: 32 4977
PstI C_TGCA'G
Cuts at: 185 371 640 971 983 1001 1202 2056 2741
Size: 186 269 331 12 18 201 854 685
Cuts at: 2741 185
Size: 2453
Fragments arranged by size:
2453 854 685 331 269 201 186 18
12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5009
PvuII CAG'CTG
Cuts at: 351 660 978 1585 1716 2434 2794 351
Size: 309 318 607 131 718 360 2566
Fragments arranged by size:
2566 718 607 360 318 309 131
RsrII CG'GwC_CG
Cuts at: 3204 3204
Size: 5009
SacI G_AGCT'C
Cuts at: 1049 2023 1049
Size: 974 4035
SacII CC_GC'GG
Cuts at: 1118 1210 1118
Size: 92 4917
SalI G'TCGA_C
Cuts at: 2046 2046
Size: 5009
SapI GCTCTTCn'nnn_
Cuts at: 3032 3242 3032
Size: 210 4799
SbfI CC_TGCA'GG
Cuts at: 1202 2056 1202
Size: 854 4155
ScaI AGT'ACT
Cuts at: 4526 4526
Size: 5009
SexAI A'CCwGG_T
Cuts at: 1025 1025
Size: 5009
SfcI C'TryA_G
Cuts at: 33 181 367 636 967 979 997 1198 2052
Size: 148 186 269 331 12 18 201 854
Cuts at: 2052 2737 3730 3921 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 854 685 331 269 201 191
186 148 18 12
SfoI GGC'GCC
Cuts at: 1088 2441 2689 1088
Size: 1353 248 3408
Fragments arranged by size:
3408 1353 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5009
SgrAI Cr'CCGG_yG
Cuts at: 1781 1781
Size: 5009
SmaI CCC'GGG
Cuts at: 736 2031 736
Size: 1295 3714
SmlI C'TyrA_G
Cuts at: 2164 3079 3571 3833 2164
Size: 915 492 262 3340
Fragments arranged by size:
3340 915 492 262
SnaBI TAC'GTA
Cuts at: 2001 2001
Size: 5009
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5009
SphI G_CATG'C
Cuts at: 2062 2562 2062
Size: 500 4509
StuI AGG'CCT
Cuts at: 144 313 144
Size: 169 4840
StyI C'CwwG_G
Cuts at: 515 563 1091 1830 1952 2143 2497 515
Size: 48 528 739 122 191 354 3027
Fragments arranged by size:
3027 739 528 354 191 122 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2592 2725 3184 3393 2592
Size: 133 459 209 4208
Fragments arranged by size:
4208 459 209 133
TatI w'GTAC_w
Cuts at: 740 4524 740
Size: 3784 1225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4526 4558 4526
Size: 32 4977
Tth111I GACn'n_nGTC
Cuts at: 2806 2806
Size: 5009
XbaI T'CTAG_A
Cuts at: 2040 2040
Size: 5009
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2504 2504
Size: 5009
XmaI C'CCGG_G
Cuts at: 734 2029 734
Size: 1295 3714
ZraI GAC'GTC
Cuts at: 360 360
Size: 5009
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AloI ApaI AscI AvrII BbvCI BmtI
Bpu10I BsiWI BsmI BsrGI BssHII BstZ17I CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SfiI SrfI SspI SwaI XhoI
XmnI