Restriction map for
pF1KSDA0111
(Circular) (Six-base) MAPSORT of: pF1KSDA0111.seq Check: 2788 from: 1 to: 4370
pF1KSDA0111 4370 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 737 1408 737
Size: 671 3699
Acc65I G'GTAC_C
Cuts at: 743 1386 743
Size: 643 3727
AclI AA'CG_TT
Cuts at: 1343 4210 1343
Size: 2867 1503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 2195 2627 3374 3642 384
Size: 1811 432 747 268 1112
Fragments arranged by size:
1811 1112 747 432 268
AflIII A'CryG_T
Cuts at: 658 1722 2826 3803 658
Size: 1064 1104 977 1225
Fragments arranged by size:
1225 1104 1064 977
AgeI A'CCGG_T
Cuts at: 2758 2758
Size: 4370
AleI CACnn'nnGTG
Cuts at: 763 763
Size: 4370
AlwNI CAG_nnn'CTG
Cuts at: 1675 3242 1675
Size: 1567 2803
ApaLI G'TGCA_C
Cuts at: 3140 3140
Size: 4370
ApoI r'AATT_y
Cuts at: 205 916 1374 3723 3979 205
Size: 711 458 2349 256 596
Fragments arranged by size:
2349 711 596 458 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 153 1390 153
Size: 1237 3133
BamHI G'GATC_C
Cuts at: 1395 1395
Size: 4370
BanI G'GyrC_C
Cuts at: 553 743 1102 1386 1800 2048 2083 553
Size: 190 359 284 414 248 35 2840
Fragments arranged by size:
2840 414 359 284 248 190 35
BanII G_rGCy'C
Cuts at: 160 1384 2414 160
Size: 1224 1030 2116
Fragments arranged by size:
2116 1224 1030
BbeI G_GCGC'C
Cuts at: 1106 1804 2052 1106
Size: 698 248 3424
Fragments arranged by size:
3424 698 248
BbsI GAAGACnn'nnnn_
Cuts at: 194 194
Size: 4370
BciVI GTATCCnnnnn_n'
Cuts at: 1047 2268 3029 4370 1047
Size: 1221 761 1341 1047
Fragments arranged by size:
1341 1221 1047 761
BclI T'GATC_A
Cuts at: 1890 1890
Size: 4370
BfrBI ATG'CAT
Cuts at: 1047 1047
Size: 4370
BglI GCCn_nnn'nGGC
Cuts at: 145 145
Size: 4370
BglII A'GATC_T
Cuts at: 1885 3922 1885
Size: 2037 2333
BlpI GC'TnA_GC
Cuts at: 1482 1482
Size: 4370
Bme1580I G_kGCm'C
Cuts at: 613 1995 2088 3144 613
Size: 1382 93 1056 1839
Fragments arranged by size:
1839 1382 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1582 1663 1998 4142 1582
Size: 81 335 2144 1810
Fragments arranged by size:
2144 1810 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 599 737 823 400
Size: 199 138 86 3947
Fragments arranged by size:
3947 199 138 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 1546 2425 2917 3215 431
Size: 1115 879 492 298 1586
Fragments arranged by size:
1586 1115 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 205 4074 205
Size: 3869 501
BsaAI yAC'GTr
Cuts at: 661 1362 1723 661
Size: 701 361 3308
Fragments arranged by size:
3308 701 361
BsaBI GATnn'nnATC
Cuts at: 6 1884 3921 6
Size: 1878 2037 455
Fragments arranged by size:
2037 1878 455
BsaHI Gr'CG_yC
Cuts at: 1103 1801 2049 2751 4247 1103
Size: 698 248 702 1496 1226
Fragments arranged by size:
1496 1226 702 698 248
BsaWI w'CCGG_w
Cuts at: 1094 1559 2080 2758 3032 3179 3590 1094
Size: 465 521 678 274 147 411 1874
Fragments arranged by size:
1874 678 521 465 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1081 1111 1215 1245 1081
Size: 30 104 30 4206
Fragments arranged by size:
4206 104 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 235
Size: 4370
BseYI C'CCAG_C
Cuts at: 1795 2553 3130 3626 1795
Size: 758 577 496 2539
Fragments arranged by size:
2539 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 505
Size: 4370
BsiEI CG_ry'CG
Cuts at: 104 1099 1242 1912 1958 3166 104
Size: 995 143 670 46 1208 1308
Fragments arranged by size:
1308 1208 995 670 143 46
BsiHKAI G_wGCw'C
Cuts at: 1384 2162 2352 3144 1384
Size: 778 190 792 2610
Fragments arranged by size:
2610 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 160 613 1384 1995 2088 2162 2352 2414 3144
Size: 453 771 611 93 74 190 62 730
Cuts at: 3144 160
Size: 1386
Fragments arranged by size:
1386 771 730 611 453 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3590 3590
Size: 4370
BspHI T'CATG_A
Cuts at: 821 1082 3546 821
Size: 261 2464 1645
Fragments arranged by size:
2464 1645 261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 755 1420 1634 1936 2317 278
Size: 477 665 214 302 381 2331
Fragments arranged by size:
2331 665 477 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1071 2662 2716 4200 4365 50
Size: 1021 1591 54 1484 165 55
Fragments arranged by size:
1591 1484 1021 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2282 2282
Size: 4370
BsrFI r'CCGG_y
Cuts at: 2368 2549 2758 2368
Size: 181 209 3980
Fragments arranged by size:
3980 209 181
BsrGI T'GTAC_A
Cuts at: 1205 1205
Size: 4370
BssSI C'ACGA_G
Cuts at: 793 2641 2999 793
Size: 1848 358 2164
Fragments arranged by size:
2164 1848 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1747 1747
Size: 4370
BstBI TT'CG_AA
Cuts at: 110 2731 110
Size: 2621 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 765 1146 765
Size: 381 3989
BstYI r'GATC_y
Cuts at: 1 488 1395 1862 1885 2220 2466 3478 3564
Size: 487 907 467 23 335 246 1012 86
Cuts at: 3564 3576 3922 1
Size: 12 346 449
Fragments arranged by size:
1012 907 487 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 738 738
Size: 4370
Bsu36I CC'TnA_GG
Cuts at: 652 1157 652
Size: 505 3865
BtgI C'CryG_G
Cuts at: 125 134 251 125
Size: 9 117 4244
Fragments arranged by size:
4244 117 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 345 2320 2501 2664 345
Size: 1975 181 163 2051
Fragments arranged by size:
2051 1975 181 163
BtsI GCAGTG_nn'
Cuts at: 902 1613 902
Size: 711 3659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1305 1340 1305
Size: 35 4335
DraI TTT'AAA
Cuts at: 1369 1369
Size: 4370
DraIII CAC_nnn'GTG
Cuts at: 929 929
Size: 4370
DrdI GACnn_nn'nnGTC
Cuts at: 2076 2934 2076
Size: 858 3512
EaeI y'GGCC_r
Cuts at: 137 1239 1253 1955 2129 2520 2547 3892 137
Size: 1102 14 702 174 391 27 1345 615
Fragments arranged by size:
1345 1102 702 615 391 174 27 14
EagI C'GGCC_G
Cuts at: 1239 1955 1239
Size: 716 3654
EarI CTCTTCn'nnn_
Cuts at: 902 2393 2603 902
Size: 1491 210 2669
Fragments arranged by size:
2669 1491 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2578 2888 3034 4180 2578
Size: 310 146 1146 2768
Fragments arranged by size:
2768 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1382 1382
Size: 4370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 400 599 737 823 2195 2627 3374 3642
Size: 16 199 138 86 1372 432 747 268
Cuts at: 3642 384
Size: 1112
Fragments arranged by size:
1372 1112 747 432 268 199 138 86
16
EcoNI CCTnn'n_nnAGG
Cuts at: 262 262
Size: 4370
EcoO109I rG'GnC_Cy
Cuts at: 267 1509 267
Size: 1242 3128
EcoRI G'AATT_C
Cuts at: 205 1374 205
Size: 1169 3201
EcoRV GAT'ATC
Cuts at: 1299 1299
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 4370
FspI TGC'GCA
Cuts at: 2151 2151
Size: 4370
HaeII r_GCGC'y
Cuts at: 1106 1804 2052 3074 4053 1106
Size: 698 248 1022 979 1423
Fragments arranged by size:
1423 1022 979 698 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 344 376 447 479 942 974 1060 1081 1092
Size: 32 71 32 463 32 86 21 11
Cuts at: 1092 1113 1215 1247 2212 2242 2244 2274 344
Size: 21 102 32 965 30 2 30 2440
Fragments arranged by size:
2440 965 463 102 86 71 32 32
32 32 30 30 21 21 11 2
HincII GTy'rAC
Cuts at: 1409 1409
Size: 4370
HindIII A'AGCT_T
Cuts at: 449 449
Size: 4370
KasI G'GCGC_C
Cuts at: 1102 1800 2048 1102
Size: 698 248 3424
Fragments arranged by size:
3424 698 248
KpnI G_GTAC'C
Cuts at: 747 1390 747
Size: 643 3727
MluI A'CGCG_T
Cuts at: 3803 3803
Size: 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1127 3041 3225 1127
Size: 1914 184 2272
Fragments arranged by size:
2272 1914 184
MscI TGG'CCA
Cuts at: 1255 2131 1255
Size: 876 3494
MslI CAynn'nnrTG
Cuts at: 763 844 2359 2486 763
Size: 81 1515 127 2647
Fragments arranged by size:
2647 1515 127 81
MspA1I CmG'CkG
Cuts at: 1481 1795 2155 3168 3413 1481
Size: 314 360 1013 245 2438
Fragments arranged by size:
2438 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2551 2551
Size: 4370
NarI GG'CG_CC
Cuts at: 1103 1801 2049 1103
Size: 698 248 3424
Fragments arranged by size:
3424 698 248
NcoI C'CATG_G
Cuts at: 125 251 125
Size: 126 4244
NgoMIV G'CCGG_C
Cuts at: 2549 2549
Size: 4370
NsiI A_TGCA'T
Cuts at: 1049 1049
Size: 4370
NspI r_CATG'y
Cuts at: 601 1059 1423 1923 2830 601
Size: 458 364 500 907 2141
Fragments arranged by size:
2141 907 500 458 364
PciI A'CATG_T
Cuts at: 2826 2826
Size: 4370
PflMI CCAn_nnn'nTGG
Cuts at: 423 423
Size: 4370
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PmlI CAC'GTG
Cuts at: 661 661
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 4338
PpuMI rG'GwC_Cy
Cuts at: 267 267
Size: 4370
PstI C_TGCA'G
Cuts at: 1417 2102 1417
Size: 685 3685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 1795 2155 1795
Size: 360 4010
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4370
SacI G_AGCT'C
Cuts at: 1384 1384
Size: 4370
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4370
SapI GCTCTTCn'nnn_
Cuts at: 2393 2603 2393
Size: 210 4160
SbfI CC_TGCA'GG
Cuts at: 1417 1417
Size: 4370
ScaI AGT'ACT
Cuts at: 1309 3887 1309
Size: 2578 1792
SfcI C'TryA_G
Cuts at: 33 1124 1413 2098 3091 3282 33
Size: 1091 289 685 993 191 1121
Fragments arranged by size:
1121 1091 993 685 289 191
SfoI GGC'GCC
Cuts at: 1104 1802 2050 1104
Size: 698 248 3424
Fragments arranged by size:
3424 698 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 1392 1392
Size: 4370
SmlI C'TyrA_G
Cuts at: 446 1525 2440 2932 3194 446
Size: 1079 915 492 262 1622
Fragments arranged by size:
1622 1079 915 492 262
SnaBI TAC'GTA
Cuts at: 1362 1362
Size: 4370
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 1423 1923 1423
Size: 500 3870
StyI C'CwwG_G
Cuts at: 125 251 1504 1858 125
Size: 126 1253 354 2637
Fragments arranged by size:
2637 1253 354 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 841 1953 2086 2545 2754 841
Size: 1112 133 459 209 2457
Fragments arranged by size:
2457 1112 459 209 133
TatI w'GTAC_w
Cuts at: 1205 1307 3885 1205
Size: 102 2578 1690
Fragments arranged by size:
2578 1690 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3887 3919 3887
Size: 32 4338
Tth111I GACn'n_nGTC
Cuts at: 2167 2167
Size: 4370
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 222 474 544 990 1865 141
Size: 81 252 70 446 875 2646
Fragments arranged by size:
2646 875 446 252 81 70
XmaI C'CCGG_G
Cuts at: 1390 1390
Size: 4370
XmnI GAAnn'nnTTC
Cuts at: 1090 1090
Size: 4370
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BssHII BstEII ClaI FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PfoI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI
ZraI