Restriction map for
pF1KSDA0108
(Circular) (Six-base) MAPSORT of: pF1KSDA0108.seq Check: 9696 from: 1 to: 3836
pF1KSDA0108 3836 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 874 874
Size: 3836
Acc65I G'GTAC_C
Cuts at: 852 852
Size: 3836
AclI AA'CG_TT
Cuts at: 3676 3676
Size: 3836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1661 2093 2840 3108 1661
Size: 432 747 268 2389
Fragments arranged by size:
2389 747 432 268
AflIII A'CryG_T
Cuts at: 1188 2292 3269 1188
Size: 1104 977 1755
Fragments arranged by size:
1755 1104 977
AgeI A'CCGG_T
Cuts at: 161 2224 161
Size: 2063 1773
AlwNI CAG_nnn'CTG
Cuts at: 1141 2708 1141
Size: 1567 2269
ApaLI G'TGCA_C
Cuts at: 2606 2606
Size: 3836
ApoI r'AATT_y
Cuts at: 840 3189 3445 840
Size: 2349 256 1231
Fragments arranged by size:
2349 1231 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
AvaI C'yCGr_G
Cuts at: 856 856
Size: 3836
BamHI G'GATC_C
Cuts at: 861 861
Size: 3836
BanI G'GyrC_C
Cuts at: 852 1266 1514 1549 852
Size: 414 248 35 3139
Fragments arranged by size:
3139 414 248 35
BanII G_rGCy'C
Cuts at: 850 1880 850
Size: 1030 2806
BbeI G_GCGC'C
Cuts at: 1270 1518 1270
Size: 248 3588
BciVI GTATCCnnnnn_n'
Cuts at: 1734 2495 3836 1734
Size: 761 1341 1734
Fragments arranged by size:
1734 1341 761
BclI T'GATC_A
Cuts at: 1356 1356
Size: 3836
BglII A'GATC_T
Cuts at: 1351 3388 1351
Size: 2037 1799
BlpI GC'TnA_GC
Cuts at: 948 948
Size: 3836
Bme1580I G_kGCm'C
Cuts at: 1461 1554 2610 1461
Size: 93 1056 2687
Fragments arranged by size:
2687 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1048 1129 1464 3608 1048
Size: 81 335 2144 1276
Fragments arranged by size:
2144 1276 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 3836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 582
Size: 3836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1891 2383 2681 1012
Size: 879 492 298 2167
Fragments arranged by size:
2167 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3540 3540
Size: 3836
BsaAI yAC'GTr
Cuts at: 828 1189 828
Size: 361 3475
BsaBI GATnn'nnATC
Cuts at: 6 1350 3387 6
Size: 1344 2037 455
Fragments arranged by size:
2037 1344 455
BsaHI Gr'CG_yC
Cuts at: 1267 1515 2217 3713 1267
Size: 248 702 1496 1390
Fragments arranged by size:
1496 1390 702 248
BsaWI w'CCGG_w
Cuts at: 161 1025 1546 2224 2498 2645 3056 161
Size: 864 521 678 274 147 411 941
Fragments arranged by size:
941 864 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 737 737
Size: 3836
BseYI C'CCAG_C
Cuts at: 1261 2019 2596 3092 1261
Size: 758 577 496 2005
Fragments arranged by size:
2005 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1378 1424 2632 104
Size: 1274 46 1208 1308
Fragments arranged by size:
1308 1274 1208 46
BsiHKAI G_wGCw'C
Cuts at: 850 1628 1818 2610 850
Size: 778 190 792 2076
Fragments arranged by size:
2076 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 387 387
Size: 3836
Bsp1286I G_dGCh'C
Cuts at: 850 1461 1554 1628 1818 1880 2610 850
Size: 611 93 74 190 62 730 2076
Fragments arranged by size:
2076 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3056 3056
Size: 3836
BspHI T'CATG_A
Cuts at: 3012 3012
Size: 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 828 886 1100 1402 1783 828
Size: 58 214 302 381 2881
Fragments arranged by size:
2881 381 302 214 58
BsrBI CCG'CTC
Cuts at: 50 2128 2182 3666 3831 50
Size: 2078 54 1484 165 55
Fragments arranged by size:
2078 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1748 1748
Size: 3836
BsrFI r'CCGG_y
Cuts at: 161 1834 2015 2224 161
Size: 1673 181 209 1773
Fragments arranged by size:
1773 1673 209 181
BssSI C'ACGA_G
Cuts at: 2107 2465 2107
Size: 358 3478
BstAPI GCAn_nnn'nTGC
Cuts at: 179 1213 179
Size: 1034 2802
BstBI TT'CG_AA
Cuts at: 110 2197 110
Size: 2087 1749
BstYI r'GATC_y
Cuts at: 1 861 1328 1351 1686 1932 2944 3030 3042
Size: 860 467 23 335 246 1012 86 12
Cuts at: 3042 3388 1
Size: 346 449
Fragments arranged by size:
1012 860 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 3836
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1786 1967 2130 1786
Size: 181 163 3492
Fragments arranged by size:
3492 181 163
BtsI GCAGTG_nn'
Cuts at: 162 1079 162
Size: 917 2919
DraI TTT'AAA
Cuts at: 835 835
Size: 3836
DraIII CAC_nnn'GTG
Cuts at: 179 179
Size: 3836
DrdI GACnn_nn'nnGTC
Cuts at: 1542 2400 1542
Size: 858 2978
EaeI y'GGCC_r
Cuts at: 776 1421 1595 1986 2013 3358 776
Size: 645 174 391 27 1345 1254
Fragments arranged by size:
1345 1254 645 391 174 27
EagI C'GGCC_G
Cuts at: 1421 1421
Size: 3836
EarI CTCTTCn'nnn_
Cuts at: 1859 2069 1859
Size: 210 3626
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2044 2354 2500 3646 2044
Size: 310 146 1146 2234
Fragments arranged by size:
2234 1146 310 146
EcoICRI GAG'CTC
Cuts at: 848 848
Size: 3836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 582 1661 2093 2840 3108 582
Size: 1079 432 747 268 1310
Fragments arranged by size:
1310 1079 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 224 975 224
Size: 751 3085
EcoRI G'AATT_C
Cuts at: 840 840
Size: 3836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3836
FspI TGC'GCA
Cuts at: 1617 1617
Size: 3836
HaeII r_GCGC'y
Cuts at: 1270 1518 2540 3519 1270
Size: 248 1022 979 1587
Fragments arranged by size:
1587 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1678 1708 1710 1740 1678
Size: 30 2 30 3774
Fragments arranged by size:
3774 30 30 2
HincII GTy'rAC
Cuts at: 306 875 306
Size: 569 3267
KasI G'GCGC_C
Cuts at: 1266 1514 1266
Size: 248 3588
KpnI G_GTAC'C
Cuts at: 856 856
Size: 3836
MluI A'CGCG_T
Cuts at: 3269 3269
Size: 3836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2507 2691 2507
Size: 184 3652
MscI TGG'CCA
Cuts at: 1597 1597
Size: 3836
MslI CAynn'nnrTG
Cuts at: 1825 1952 1825
Size: 127 3709
MspA1I CmG'CkG
Cuts at: 301 602 947 1261 1621 2634 2879 301
Size: 301 345 314 360 1013 245 1258
Fragments arranged by size:
1258 1013 360 345 314 301 245
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 3836
NarI GG'CG_CC
Cuts at: 1267 1515 1267
Size: 248 3588
NcoI C'CATG_G
Cuts at: 125 125
Size: 3836
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 3836
NspI r_CATG'y
Cuts at: 889 1389 2296 889
Size: 500 907 2429
Fragments arranged by size:
2429 907 500
PciI A'CATG_T
Cuts at: 2292 2292
Size: 3836
PmeI GTTT'AAAC
Cuts at: 835 835
Size: 3836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 3804
PpuMI rG'GwC_Cy
Cuts at: 224 224
Size: 3836
PsiI TTA'TAA
Cuts at: 400 400
Size: 3836
PstI C_TGCA'G
Cuts at: 883 1568 883
Size: 685 3151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3836
PvuII CAG'CTG
Cuts at: 301 602 1261 1621 301
Size: 301 659 360 2516
Fragments arranged by size:
2516 659 360 301
RsrII CG'GwC_CG
Cuts at: 2031 2031
Size: 3836
SacI G_AGCT'C
Cuts at: 850 850
Size: 3836
SalI G'TCGA_C
Cuts at: 873 873
Size: 3836
SapI GCTCTTCn'nnn_
Cuts at: 1859 2069 1859
Size: 210 3626
SbfI CC_TGCA'GG
Cuts at: 883 883
Size: 3836
ScaI AGT'ACT
Cuts at: 3353 3353
Size: 3836
SexAI A'CCwGG_T
Cuts at: 226 226
Size: 3836
SfcI C'TryA_G
Cuts at: 33 168 879 1564 2557 2748 33
Size: 135 711 685 993 191 1121
Fragments arranged by size:
1121 993 711 685 191 135
SfoI GGC'GCC
Cuts at: 1268 1516 1268
Size: 248 3588
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
SmaI CCC'GGG
Cuts at: 858 858
Size: 3836
SmlI C'TyrA_G
Cuts at: 991 1906 2398 2660 991
Size: 915 492 262 2167
Fragments arranged by size:
2167 915 492 262
SnaBI TAC'GTA
Cuts at: 828 828
Size: 3836
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3836
SphI G_CATG'C
Cuts at: 889 1389 889
Size: 500 3336
StyI C'CwwG_G
Cuts at: 125 590 970 1324 125
Size: 465 380 354 2637
Fragments arranged by size:
2637 465 380 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1419 1552 2011 2220 1419
Size: 133 459 209 3035
Fragments arranged by size:
3035 459 209 133
TatI w'GTAC_w
Cuts at: 3351 3351
Size: 3836
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 141 173 3353 3385 141
Size: 32 3180 32 592
Fragments arranged by size:
3180 592 32 32
Tth111I GACn'n_nGTC
Cuts at: 484 1633 484
Size: 1149 2687
XbaI T'CTAG_A
Cuts at: 867 867
Size: 3836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1331 1331
Size: 3836
XmaI C'CCGG_G
Cuts at: 856 856
Size: 3836
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI SspI StuI SwaI XhoI XmnI ZraI