Restriction map for
pF1KSDA0106
(Circular) (Six-base) MAPSORT of: pF1KSDA0106.seq Check: 5930 from: 1 to: 3809
pF1KSDA0106 3809 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 847 847
Size: 3809
Acc65I G'GTAC_C
Cuts at: 825 825
Size: 3809
AclI AA'CG_TT
Cuts at: 3649 3649
Size: 3809
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 574 728 1634 2066 2813 3081 574
Size: 154 906 432 747 268 1302
Fragments arranged by size:
1302 906 747 432 268 154
AflIII A'CryG_T
Cuts at: 1161 2265 3242 1161
Size: 1104 977 1728
Fragments arranged by size:
1728 1104 977
AgeI A'CCGG_T
Cuts at: 2197 2197
Size: 3809
AlwNI CAG_nnn'CTG
Cuts at: 1114 2681 1114
Size: 1567 2242
ApaLI G'TGCA_C
Cuts at: 264 2579 264
Size: 2315 1494
ApoI r'AATT_y
Cuts at: 304 813 3162 3418 304
Size: 509 2349 256 695
Fragments arranged by size:
2349 695 509 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3809
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3809
AvaI C'yCGr_G
Cuts at: 145 245 829 145
Size: 100 584 3125
Fragments arranged by size:
3125 584 100
BamHI G'GATC_C
Cuts at: 477 834 477
Size: 357 3452
BanI G'GyrC_C
Cuts at: 297 825 1239 1487 1522 297
Size: 528 414 248 35 2584
Fragments arranged by size:
2584 528 414 248 35
BanII G_rGCy'C
Cuts at: 407 758 823 1853 407
Size: 351 65 1030 2363
Fragments arranged by size:
2363 1030 351 65
BbeI G_GCGC'C
Cuts at: 1243 1491 1243
Size: 248 3561
BbsI GAAGACnn'nnnn_
Cuts at: 735 735
Size: 3809
BciVI GTATCCnnnnn_n'
Cuts at: 1707 2468 3809 1707
Size: 761 1341 1707
Fragments arranged by size:
1707 1341 761
BclI T'GATC_A
Cuts at: 1329 1329
Size: 3809
BglII A'GATC_T
Cuts at: 1324 3361 1324
Size: 2037 1772
BlpI GC'TnA_GC
Cuts at: 921 921
Size: 3809
Bme1580I G_kGCm'C
Cuts at: 268 1434 1527 2583 268
Size: 1166 93 1056 1494
Fragments arranged by size:
1494 1166 1056 93
BmgBI CAC'GTC
Cuts at: 153 153
Size: 3809
BmrI ACTGGGnnnn_n'
Cuts at: 253 652 1021 1102 1437 3581 253
Size: 399 369 81 335 2144 481
Fragments arranged by size:
2144 481 399 369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 3809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 610 390
Size: 220 3589
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 1864 2356 2654 985
Size: 879 492 298 2140
Fragments arranged by size:
2140 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3513 3513
Size: 3809
BsaAI yAC'GTr
Cuts at: 801 1162 801
Size: 361 3448
BsaBI GATnn'nnATC
Cuts at: 6 443 1323 3360 6
Size: 437 880 2037 455
Fragments arranged by size:
2037 880 455 437
BsaHI Gr'CG_yC
Cuts at: 1240 1488 2190 3686 1240
Size: 248 702 1496 1363
Fragments arranged by size:
1496 1363 702 248
BsaWI w'CCGG_w
Cuts at: 998 1519 2197 2471 2618 3029 998
Size: 521 678 274 147 411 1778
Fragments arranged by size:
1778 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 573 791 1234 1992 2569 3065 573
Size: 218 443 758 577 496 1317
Fragments arranged by size:
1317 758 577 496 443 218
BsiEI CG_ry'CG
Cuts at: 104 189 1351 1397 2605 104
Size: 85 1162 46 1208 1308
Fragments arranged by size:
1308 1208 1162 85 46
BsiHKAI G_wGCw'C
Cuts at: 268 758 823 1601 1791 2583 268
Size: 490 65 778 190 792 1494
Fragments arranged by size:
1494 792 778 490 190 65
BsmI GAATG_Cn'
Cuts at: 227 227
Size: 3809
Bsp1286I G_dGCh'C
Cuts at: 268 407 758 823 1434 1527 1601 1791 1853
Size: 139 351 65 611 93 74 190 62
Cuts at: 1853 2583 268
Size: 730 1494
Fragments arranged by size:
1494 730 611 351 190 139 93 74
65 62
BspEI T'CCGG_A
Cuts at: 3029 3029
Size: 3809
BspHI T'CATG_A
Cuts at: 2985 2985
Size: 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 859 1073 1375 1756 859
Size: 214 302 381 2912
Fragments arranged by size:
2912 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2101 2155 3639 3804 50
Size: 2051 54 1484 165 55
Fragments arranged by size:
2051 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1721 1721
Size: 3809
BsrFI r'CCGG_y
Cuts at: 1807 1988 2197 1807
Size: 181 209 3419
Fragments arranged by size:
3419 209 181
BssSI C'ACGA_G
Cuts at: 518 2080 2438 518
Size: 1562 358 1889
Fragments arranged by size:
1889 1562 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1186 1186
Size: 3809
BstBI TT'CG_AA
Cuts at: 110 2170 110
Size: 2060 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 465 665 465
Size: 200 3609
BstYI r'GATC_y
Cuts at: 1 477 834 1301 1324 1659 1905 2917 3003
Size: 476 357 467 23 335 246 1012 86
Cuts at: 3003 3015 3361 1
Size: 12 346 449
Fragments arranged by size:
1012 476 467 449 357 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1759 1940 2103 1759
Size: 181 163 3465
Fragments arranged by size:
3465 181 163
BtsI GCAGTG_nn'
Cuts at: 1052 1052
Size: 3809
ClaI AT'CG_AT
Cuts at: 434 434
Size: 3809
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 258 293 642 677 258
Size: 35 349 35 3390
Fragments arranged by size:
3390 349 35 35
DraI TTT'AAA
Cuts at: 808 808
Size: 3809
DrdI GACnn_nn'nnGTC
Cuts at: 1515 2373 1515
Size: 858 2951
EaeI y'GGCC_r
Cuts at: 186 1394 1568 1959 1986 3331 186
Size: 1208 174 391 27 1345 664
Fragments arranged by size:
1345 1208 664 391 174 27
EagI C'GGCC_G
Cuts at: 186 1394 186
Size: 1208 2601
EarI CTCTTCn'nnn_
Cuts at: 238 395 1832 2042 238
Size: 157 1437 210 2005
Fragments arranged by size:
2005 1437 210 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2017 2327 2473 3619 2017
Size: 310 146 1146 2207
Fragments arranged by size:
2207 1146 310 146
EcoICRI GAG'CTC
Cuts at: 756 821 756
Size: 65 3744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 574 610 728 1634 2066 2813 3081 390
Size: 184 36 118 906 432 747 268 1118
Fragments arranged by size:
1118 906 747 432 268 184 118 36
EcoO109I rG'GnC_Cy
Cuts at: 948 948
Size: 3809
EcoRI G'AATT_C
Cuts at: 813 813
Size: 3809
EcoRV GAT'ATC
Cuts at: 381 381
Size: 3809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1828 1828
Size: 3809
FspI TGC'GCA
Cuts at: 1590 1590
Size: 3809
HaeII r_GCGC'y
Cuts at: 1243 1491 2513 3492 1243
Size: 248 1022 979 1560
Fragments arranged by size:
1560 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 745 777 1651 1681 1683 1713 745
Size: 32 874 30 2 30 2841
Fragments arranged by size:
2841 874 32 30 30 2
HincII GTy'rAC
Cuts at: 848 848
Size: 3809
KasI G'GCGC_C
Cuts at: 1239 1487 1239
Size: 248 3561
KpnI G_GTAC'C
Cuts at: 829 829
Size: 3809
MfeI C'AATT_G
Cuts at: 393 393
Size: 3809
MluI A'CGCG_T
Cuts at: 3242 3242
Size: 3809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 721 2480 2664 721
Size: 1759 184 1866
Fragments arranged by size:
1866 1759 184
MscI TGG'CCA
Cuts at: 1570 1570
Size: 3809
MslI CAynn'nnrTG
Cuts at: 508 1798 1925 508
Size: 1290 127 2392
Fragments arranged by size:
2392 1290 127
MspA1I CmG'CkG
Cuts at: 630 920 1234 1594 2607 2852 630
Size: 290 314 360 1013 245 1587
Fragments arranged by size:
1587 1013 360 314 290 245
NaeI GCC'GGC
Cuts at: 1990 1990
Size: 3809
NarI GG'CG_CC
Cuts at: 1240 1488 1240
Size: 248 3561
NgoMIV G'CCGG_C
Cuts at: 1988 1988
Size: 3809
NspI r_CATG'y
Cuts at: 474 507 862 1362 2269 474
Size: 33 355 500 907 2014
Fragments arranged by size:
2014 907 500 355 33
PciI A'CATG_T
Cuts at: 2265 2265
Size: 3809
PmeI GTTT'AAAC
Cuts at: 808 808
Size: 3809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2948 2980 2948
Size: 32 3777
PstI C_TGCA'G
Cuts at: 856 1541 856
Size: 685 3124
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3809
PvuII CAG'CTG
Cuts at: 630 1234 1594 630
Size: 604 360 2845
Fragments arranged by size:
2845 604 360
RsrII CG'GwC_CG
Cuts at: 2004 2004
Size: 3809
SacI G_AGCT'C
Cuts at: 758 823 758
Size: 65 3744
SalI G'TCGA_C
Cuts at: 846 846
Size: 3809
SapI GCTCTTCn'nnn_
Cuts at: 395 1832 2042 395
Size: 1437 210 2162
Fragments arranged by size:
2162 1437 210
SbfI CC_TGCA'GG
Cuts at: 856 856
Size: 3809
ScaI AGT'ACT
Cuts at: 3326 3326
Size: 3809
SfcI C'TryA_G
Cuts at: 33 852 1537 2530 2721 33
Size: 819 685 993 191 1121
Fragments arranged by size:
1121 993 819 685 191
SfoI GGC'GCC
Cuts at: 1241 1489 1241
Size: 248 3561
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3809
SmaI CCC'GGG
Cuts at: 831 831
Size: 3809
SmlI C'TyrA_G
Cuts at: 964 1879 2371 2633 964
Size: 915 492 262 2140
Fragments arranged by size:
2140 915 492 262
SnaBI TAC'GTA
Cuts at: 801 801
Size: 3809
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3809
SphI G_CATG'C
Cuts at: 474 507 862 1362 474
Size: 33 355 500 2921
Fragments arranged by size:
2921 500 355 33
StyI C'CwwG_G
Cuts at: 943 1297 943
Size: 354 3455
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1392 1525 1984 2193 1392
Size: 133 459 209 3008
Fragments arranged by size:
3008 459 209 133
TatI w'GTAC_w
Cuts at: 3324 3324
Size: 3809
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 3777
Tth111I GACn'n_nGTC
Cuts at: 1606 1606
Size: 3809
XbaI T'CTAG_A
Cuts at: 840 840
Size: 3809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1304 1304
Size: 3809
XmaI C'CCGG_G
Cuts at: 829 829
Size: 3809
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI DraIII EcoNI FseI
FspAI HindIII HpaI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SspI StuI SwaI XhoI XmnI ZraI