Restriction map for
pF1KSDA0096
(Circular) (Six-base) MAPSORT of: pF1KSDA0096.seq Check: 3538 from: 1 to: 5434
pF1KSDA0096 5434 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 714 714
Size: 5434
AccI GT'mk_AC
Cuts at: 2470 2470
Size: 5434
Acc65I G'GTAC_C
Cuts at: 1987 2448 1987
Size: 461 4973
AclI AA'CG_TT
Cuts at: 5274 5274
Size: 5434
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 916 3257 3689 4438 4706 916
Size: 2341 432 749 268 1644
Fragments arranged by size:
2341 1644 749 432 268
AflIII A'CryG_T
Cuts at: 2784 3890 4867 2784
Size: 1106 977 3351
Fragments arranged by size:
3351 1106 977
AgeI A'CCGG_T
Cuts at: 3822 3822
Size: 5434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2038 2237 2038
Size: 199 5235
AlwNI CAG_nnn'CTG
Cuts at: 2737 4306 2737
Size: 1569 3865
ApaI G_GGCC'C
Cuts at: 1844 1844
Size: 5434
ApaLI G'TGCA_C
Cuts at: 4204 4204
Size: 5434
ApoI r'AATT_y
Cuts at: 616 677 2436 4787 5043 616
Size: 61 1759 2351 256 1007
Fragments arranged by size:
2351 1759 1007 256 61
AseI AT'TA_AT
Cuts at: 20 20
Size: 5434
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5434
AvaI C'yCGr_G
Cuts at: 1179 1301 1959 2452 1179
Size: 122 658 493 4161
Fragments arranged by size:
4161 658 493 122
BamHI G'GATC_C
Cuts at: 1130 2457 1130
Size: 1327 4107
BanI G'GyrC_C
Cuts at: 1404 1817 1987 2448 2862 3110 3145 1404
Size: 413 170 461 414 248 35 3693
Fragments arranged by size:
3693 461 414 413 248 170 35
BanII G_rGCy'C
Cuts at: 1732 1844 2054 2446 3476 1732
Size: 112 210 392 1030 3690
Fragments arranged by size:
3690 1030 392 210 112
BbeI G_GCGC'C
Cuts at: 2866 3114 2866
Size: 248 5186
BbsI GAAGACnn'nnnn_
Cuts at: 470 555 470
Size: 85 5349
BciVI GTATCCnnnnn_n'
Cuts at: 3330 4093 5434 3330
Size: 763 1341 3330
Fragments arranged by size:
3330 1341 763
BclI T'GATC_A
Cuts at: 803 2952 803
Size: 2149 3285
BfrBI ATG'CAT
Cuts at: 323 323
Size: 5434
BglII A'GATC_T
Cuts at: 1168 1589 2947 4986 1168
Size: 421 1358 2039 1616
Fragments arranged by size:
2039 1616 1358 421
BlpI GC'TnA_GC
Cuts at: 2544 2544
Size: 5434
Bme1580I G_kGCm'C
Cuts at: 1844 3057 3150 4208 1844
Size: 1213 93 1058 3070
Fragments arranged by size:
3070 1213 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1191 1913 2644 2725 3060 5206 1191
Size: 722 731 81 335 2146 1419
Fragments arranged by size:
2146 1419 731 722 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3799 3799
Size: 5434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 1664 1716 1935 656
Size: 1008 52 219 4155
Fragments arranged by size:
4155 1008 219 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1264 2608 3487 3981 4279 1264
Size: 1344 879 494 298 2419
Fragments arranged by size:
2419 1344 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1744 5138 1744
Size: 3394 2040
BsaAI yAC'GTr
Cuts at: 2177 2424 2785 2177
Size: 247 361 4826
Fragments arranged by size:
4826 361 247
BsaBI GATnn'nnATC
Cuts at: 6 1634 2946 4985 6
Size: 1628 1312 2039 455
Fragments arranged by size:
2039 1628 1312 455
BsaHI Gr'CG_yC
Cuts at: 1058 2863 3111 3815 5311 1058
Size: 1805 248 704 1496 1181
Fragments arranged by size:
1805 1496 1181 704 248
BsaWI w'CCGG_w
Cuts at: 1706 2621 3142 3822 4096 4243 4654 1706
Size: 915 521 680 274 147 411 2486
Fragments arranged by size:
2486 915 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1248 1278 1701 1731 1248
Size: 30 423 30 4951
Fragments arranged by size:
4951 423 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 431 463 1018 1498 431
Size: 32 555 480 4367
Fragments arranged by size:
4367 555 480 32
BseYI C'CCAG_C
Cuts at: 2011 2044 2093 2397 2857 3615 4194 4690 2011
Size: 33 49 304 460 758 579 496 2755
Fragments arranged by size:
2755 758 579 496 460 304 49 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 355
Size: 5434
BsiEI CG_ry'CG
Cuts at: 104 2974 3020 4230 104
Size: 2870 46 1210 1308
Fragments arranged by size:
2870 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1010 2054 2110 2446 3224 3414 4208 1010
Size: 1044 56 336 778 190 794 2236
Fragments arranged by size:
2236 1044 794 778 336 190 56
BsmI GAATG_Cn'
Cuts at: 2211 2211
Size: 5434
BsmBI CGTCTCn'nnnn_
Cuts at: 1049 1049
Size: 5434
Bsp1286I G_dGCh'C
Cuts at: 1010 1732 1844 2054 2110 2446 3057 3150 3224
Size: 722 112 210 56 336 611 93 74
Cuts at: 3224 3414 3476 4208 1010
Size: 190 62 732 2236
Fragments arranged by size:
2236 732 722 611 336 210 190 112
93 74 62 56
BspEI T'CCGG_A
Cuts at: 4654 4654
Size: 5434
BspHI T'CATG_A
Cuts at: 2072 4610 2072
Size: 2538 2896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 714 1646 2205 2482 2696 2998 3379 714
Size: 932 559 277 214 302 381 2769
Fragments arranged by size:
2769 932 559 381 302 277 214
BsrBI CCG'CTC
Cuts at: 50 670 3724 3778 5264 5429 50
Size: 620 3054 54 1486 165 55
Fragments arranged by size:
3054 1486 620 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1893 3344 1893
Size: 1451 3983
BsrFI r'CCGG_y
Cuts at: 1775 2005 3430 3611 3822 1775
Size: 230 1425 181 211 3387
Fragments arranged by size:
3387 1425 230 211 181
BssSI C'ACGA_G
Cuts at: 3703 4063 3703
Size: 360 5074
BstAPI GCAn_nnn'nTGC
Cuts at: 2809 2809
Size: 5434
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5434
BstXI CCAn_nnnn'nTGG
Cuts at: 2028 2101 2277 2028
Size: 73 176 5185
Fragments arranged by size:
5185 176 73
BstYI r'GATC_y
Cuts at: 1 652 882 1130 1168 1589 2457 2924 2947
Size: 651 230 248 38 421 868 467 23
Cuts at: 2947 3282 3528 4542 4628 4640 4986 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 868 651 467 449 421 346 335
248 246 230 86 38 23 12
Bsu36I CC'TnA_GG
Cuts at: 2369 2369
Size: 5434
BtgI C'CryG_G
Cuts at: 125 209 125
Size: 84 5350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3382 3563 3726 3382
Size: 181 163 5090
Fragments arranged by size:
5090 181 163
BtsI GCAGTG_nn'
Cuts at: 1857 2046 2231 2675 1857
Size: 189 185 444 4616
Fragments arranged by size:
4616 444 189 185
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1499 1534 1875 1910 1917 1952 1499
Size: 35 341 35 7 35 4981
Fragments arranged by size:
4981 341 35 35 35 7
DraI TTT'AAA
Cuts at: 2431 2431
Size: 5434
DrdI GACnn_nn'nnGTC
Cuts at: 3138 3998 3138
Size: 860 4574
EaeI y'GGCC_r
Cuts at: 470 1213 1259 1326 3017 3191 3582 3609 4956
Size: 743 46 67 1691 174 391 27 1347
Cuts at: 4956 470
Size: 948
Fragments arranged by size:
1691 1347 948 743 391 174 67 46
27
EagI C'GGCC_G
Cuts at: 3017 3017
Size: 5434
EarI CTCTTCn'nnn_
Cuts at: 902 995 1466 1561 3455 3665 902
Size: 93 471 95 1894 210 2671
Fragments arranged by size:
2671 1894 471 210 95 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 343 1455 3640 3952 4098 5244 343
Size: 1112 2185 312 146 1146 533
Fragments arranged by size:
2185 1146 1112 533 312 146
EcoICRI GAG'CTC
Cuts at: 2052 2444 2052
Size: 392 5042
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 656 916 1664 1716 1935 3257 3689 4438 4706
Size: 260 748 52 219 1322 432 749 268
Cuts at: 4706 656
Size: 1384
Fragments arranged by size:
1384 1322 749 748 432 268 260 219
52
EcoO109I rG'GnC_Cy
Cuts at: 1239 1840 1841 2217 2571 1239
Size: 601 1 376 354 4102
Fragments arranged by size:
4102 601 376 354 1
EcoRI G'AATT_C
Cuts at: 2436 2436
Size: 5434
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 917 3451 917
Size: 2534 2900
FspI TGC'GCA
Cuts at: 2388 3213 2388
Size: 825 4609
FspAI rTGC'GCAy
Cuts at: 2388 2388
Size: 5434
HaeII r_GCGC'y
Cuts at: 2866 3114 4138 5117 2866
Size: 248 1024 979 3183
Fragments arranged by size:
3183 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 903 935 1272 1304 1379 1411 1701 1733 3274
Size: 32 337 32 75 32 290 32 1541
Cuts at: 3274 3304 3306 3336 903
Size: 30 2 30 3001
Fragments arranged by size:
3001 1541 337 290 75 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 593 2471 593
Size: 1878 3556
HindIII A'AGCT_T
Cuts at: 1416 1416
Size: 5434
KasI G'GCGC_C
Cuts at: 2862 3110 2862
Size: 248 5186
KpnI G_GTAC'C
Cuts at: 1991 2452 1991
Size: 461 4973
MluI A'CGCG_T
Cuts at: 4867 4867
Size: 5434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4105 4289 4105
Size: 184 5250
MscI TGG'CCA
Cuts at: 472 1215 1328 3193 472
Size: 743 113 1865 2713
Fragments arranged by size:
2713 1865 743 113
MslI CAynn'nnrTG
Cuts at: 748 868 2347 3421 3548 748
Size: 120 1479 1074 127 2634
Fragments arranged by size:
2634 1479 1074 127 120
MspA1I CmG'CkG
Cuts at: 1736 1955 2000 2028 2361 2405 2543 2857 3217
Size: 219 45 28 333 44 138 314 360
Cuts at: 3217 4232 4477 1736
Size: 1015 245 2693
Fragments arranged by size:
2693 1015 360 333 314 245 219 138
45 44 28
NaeI GCC'GGC
Cuts at: 1777 2007 3613 1777
Size: 230 1606 3598
Fragments arranged by size:
3598 1606 230
NarI GG'CG_CC
Cuts at: 2863 3111 2863
Size: 248 5186
NcoI C'CATG_G
Cuts at: 125 125
Size: 5434
NgoMIV G'CCGG_C
Cuts at: 1775 2005 3611 1775
Size: 230 1606 3598
Fragments arranged by size:
3598 1606 230
NruI TCG'CGA
Cuts at: 3795 3795
Size: 5434
NsiI A_TGCA'T
Cuts at: 325 325
Size: 5434
NspI r_CATG'y
Cuts at: 232 2485 2985 3894 232
Size: 2253 500 909 1772
Fragments arranged by size:
2253 1772 909 500
PciI A'CATG_T
Cuts at: 3890 3890
Size: 5434
PflMI CCAn_nnn'nTGG
Cuts at: 2134 2134
Size: 5434
PfoI T'CCnGG_A
Cuts at: 308 1561 308
Size: 1253 4181
PmeI GTTT'AAAC
Cuts at: 2431 2431
Size: 5434
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4573 4605 4573
Size: 32 5402
PpuMI rG'GwC_Cy
Cuts at: 1239 2217 1239
Size: 978 4456
PspOMI G'GGCC_C
Cuts at: 1840 1840
Size: 5434
PstI C_TGCA'G
Cuts at: 711 1741 2226 2321 2479 3164 711
Size: 1030 485 95 158 685 2981
Fragments arranged by size:
2981 1030 685 485 158 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5434
PvuII CAG'CTG
Cuts at: 1736 2028 2405 2857 3217 1736
Size: 292 377 452 360 3953
Fragments arranged by size:
3953 452 377 360 292
RsrII CG'GwC_CG
Cuts at: 3627 3627
Size: 5434
SacI G_AGCT'C
Cuts at: 2054 2446 2054
Size: 392 5042
SalI G'TCGA_C
Cuts at: 2469 2469
Size: 5434
SapI GCTCTTCn'nnn_
Cuts at: 3455 3665 3455
Size: 210 5224
SbfI CC_TGCA'GG
Cuts at: 2479 2479
Size: 5434
ScaI AGT'ACT
Cuts at: 4951 4951
Size: 5434
SfcI C'TryA_G
Cuts at: 33 707 874 1737 2222 2317 2475 3160 4155
Size: 674 167 863 485 95 158 685 995
Cuts at: 4155 4346 33
Size: 191 1121
Fragments arranged by size:
1121 995 863 685 674 485 191 167
158 95
SfoI GGC'GCC
Cuts at: 2864 3112 2864
Size: 248 5186
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5434
SmaI CCC'GGG
Cuts at: 2454 2454
Size: 5434
SmlI C'TyrA_G
Cuts at: 1243 2587 3502 3996 4258 1243
Size: 1344 915 494 262 2419
Fragments arranged by size:
2419 1344 915 494 262
SnaBI TAC'GTA
Cuts at: 2424 2424
Size: 5434
SpeI A'CTAG_T
Cuts at: 67 330 67
Size: 263 5171
SphI G_CATG'C
Cuts at: 2485 2985 2485
Size: 500 4934
StuI AGG'CCT
Cuts at: 1343 1343
Size: 5434
StyI C'CwwG_G
Cuts at: 125 188 925 2566 2920 125
Size: 63 737 1641 354 2639
Fragments arranged by size:
2639 1641 737 354 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 391 3015 3148 3607 3818 391
Size: 2624 133 459 211 2007
Fragments arranged by size:
2624 2007 459 211 133
TatI w'GTAC_w
Cuts at: 2114 4949 2114
Size: 2835 2599
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1248 1280 4951 4983 1248
Size: 32 3671 32 1699
Fragments arranged by size:
3671 1699 32 32
Tth111I GACn'n_nGTC
Cuts at: 1315 3229 1315
Size: 1914 3520
XbaI T'CTAG_A
Cuts at: 399 2463 399
Size: 2064 3370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1899 2274 2927 1899
Size: 375 653 4406
Fragments arranged by size:
4406 653 375
XmaI C'CCGG_G
Cuts at: 2452 2452
Size: 5434
XmnI GAAnn'nnTTC
Cuts at: 897 897
Size: 5434
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I BsiWI
BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoNI EcoRV
FseI HpaI MfeI NdeI NheI NotI PacI PasI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SrfI SspI SwaI XhoI ZraI