Restriction map for
pF1KSDA0093
(Circular) (Six-base) MAPSORT of: pF1KSDA0093.seq Check: 6810 from: 1 to: 5839
pF1KSDA0093 5839 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1347 1347
Size: 5839
AccI GT'mk_AC
Cuts at: 2875 2875
Size: 5839
Acc65I G'GTAC_C
Cuts at: 2853 2853
Size: 5839
AclI AA'CG_TT
Cuts at: 5679 5679
Size: 5839
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1852 2581 2767 3662 4094 4843 5111 1852
Size: 729 186 895 432 749 268 2580
Fragments arranged by size:
2580 895 749 729 432 268 186
AflIII A'CryG_T
Cuts at: 3189 4295 5272 3189
Size: 1106 977 3756
Fragments arranged by size:
3756 1106 977
AgeI A'CCGG_T
Cuts at: 4227 4227
Size: 5839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2390 2390
Size: 5839
AlwNI CAG_nnn'CTG
Cuts at: 664 817 3142 4711 664
Size: 153 2325 1569 1792
Fragments arranged by size:
2325 1792 1569 153
ApaLI G'TGCA_C
Cuts at: 4609 4609
Size: 5839
ApoI r'AATT_y
Cuts at: 174 1053 1490 1750 2423 2841 5192 5448 174
Size: 879 437 260 673 418 2351 256 565
Fragments arranged by size:
2351 879 673 565 437 418 260 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5839
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5839
AvaI C'yCGr_G
Cuts at: 2618 2857 2618
Size: 239 5600
AvrII C'CTAG_G
Cuts at: 432 432
Size: 5839
BamHI G'GATC_C
Cuts at: 2862 2862
Size: 5839
BanI G'GyrC_C
Cuts at: 2622 2853 3267 3515 3550 2622
Size: 231 414 248 35 4911
Fragments arranged by size:
4911 414 248 231 35
BanII G_rGCy'C
Cuts at: 158 1084 2718 2851 3881 158
Size: 926 1634 133 1030 2116
Fragments arranged by size:
2116 1634 1030 926 133
BbeI G_GCGC'C
Cuts at: 3271 3519 3271
Size: 248 5591
BbsI GAAGACnn'nnnn_
Cuts at: 902 1793 902
Size: 891 4948
BbvCI CC'TCA_GC
Cuts at: 371 620 371
Size: 249 5590
BciVI GTATCCnnnnn_n'
Cuts at: 3735 4498 5839 3735
Size: 763 1341 3735
Fragments arranged by size:
3735 1341 763
BclI T'GATC_A
Cuts at: 3357 3357
Size: 5839
BglII A'GATC_T
Cuts at: 3352 5391 3352
Size: 2039 3800
BlpI GC'TnA_GC
Cuts at: 2949 2949
Size: 5839
Bme1580I G_kGCm'C
Cuts at: 1676 3462 3555 4613 1676
Size: 1786 93 1058 2902
Fragments arranged by size:
2902 1786 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 930 2473 3049 3130 3465 5611 930
Size: 1543 576 81 335 2146 1158
Fragments arranged by size:
2146 1543 1158 576 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4204 4204
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 692 1538 1665 2508 181
Size: 511 846 127 843 3512
Fragments arranged by size:
3512 846 843 511 127
Bpu10I CC'TnA_GC
Cuts at: 371 620 371
Size: 249 5590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1284 3013 3892 4386 4684 1284
Size: 1729 879 494 298 2439
Fragments arranged by size:
2439 1729 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 483 1276 5543 483
Size: 793 4267 779
Fragments arranged by size:
4267 793 779
BsaAI yAC'GTr
Cuts at: 255 2829 3190 255
Size: 2574 361 2904
Fragments arranged by size:
2904 2574 361
BsaBI GATnn'nnATC
Cuts at: 6 3351 5390 6
Size: 3345 2039 455
Fragments arranged by size:
3345 2039 455
BsaHI Gr'CG_yC
Cuts at: 3268 3516 4220 5716 3268
Size: 248 704 1496 3391
Fragments arranged by size:
3391 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1796 3026 3547 4227 4501 4648 5059 1796
Size: 1230 521 680 274 147 411 2576
Fragments arranged by size:
2576 1230 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 689 1898 689
Size: 1209 4630
BseYI C'CCAG_C
Cuts at: 638 1364 1403 3262 4020 4599 5095 638
Size: 726 39 1859 758 579 496 1382
Fragments arranged by size:
1859 1382 758 726 579 496 39
BsiEI CG_ry'CG
Cuts at: 104 3379 3425 4635 104
Size: 3275 46 1210 1308
Fragments arranged by size:
3275 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1084 2718 2851 3629 3819 4613 1084
Size: 1634 133 778 190 794 2310
Fragments arranged by size:
2310 1634 794 778 190 133
BsmI GAATG_Cn'
Cuts at: 1035 1035
Size: 5839
Bsp1286I G_dGCh'C
Cuts at: 158 1084 1676 2718 2851 3462 3555 3629 3819
Size: 926 592 1042 133 611 93 74 190
Cuts at: 3819 3881 4613 158
Size: 62 732 1384
Fragments arranged by size:
1384 1042 926 732 611 592 190 133
93 74 62
BspEI T'CCGG_A
Cuts at: 5059 5059
Size: 5839
BspHI T'CATG_A
Cuts at: 2139 5015 2139
Size: 2876 2963
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1347 2887 3101 3403 3784 1347
Size: 1540 214 302 381 3402
Fragments arranged by size:
3402 1540 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4129 4183 5669 5834 50
Size: 4079 54 1486 165 55
Fragments arranged by size:
4079 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3749 3749
Size: 5839
BsrFI r'CCGG_y
Cuts at: 380 3835 4016 4227 380
Size: 3455 181 211 1992
Fragments arranged by size:
3455 1992 211 181
BsrGI T'GTAC_A
Cuts at: 1266 1266
Size: 5839
BssSI C'ACGA_G
Cuts at: 4108 4468 4108
Size: 360 5479
BstAPI GCAn_nnn'nTGC
Cuts at: 850 3214 850
Size: 2364 3475
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5839
BstEII G'GTnAC_C
Cuts at: 1359 1359
Size: 5839
BstXI CCAn_nnnn'nTGG
Cuts at: 1184 1184
Size: 5839
BstYI r'GATC_y
Cuts at: 1 1476 1632 2412 2862 3329 3352 3687 3933
Size: 1475 156 780 450 467 23 335 246
Cuts at: 3933 4947 5033 5045 5391 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1475 1014 780 467 450 449 346 335
246 156 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 797 2218 797
Size: 1421 4418
BtgI C'CryG_G
Cuts at: 125 125
Size: 5839
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3787 3968 4131 3787
Size: 181 163 5495
Fragments arranged by size:
5495 181 163
BtsI GCAGTG_nn'
Cuts at: 1676 3080 1676
Size: 1404 4435
DraI TTT'AAA
Cuts at: 2382 2836 2382
Size: 454 5385
DraIII CAC_nnn'GTG
Cuts at: 850 850
Size: 5839
DrdI GACnn_nn'nnGTC
Cuts at: 3543 4403 3543
Size: 860 4979
EaeI y'GGCC_r
Cuts at: 3422 3596 3987 4014 5361 3422
Size: 174 391 27 1347 3900
Fragments arranged by size:
3900 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3422 3422
Size: 5839
EarI CTCTTCn'nnn_
Cuts at: 713 1095 1559 3860 4070 713
Size: 382 464 2301 210 2482
Fragments arranged by size:
2482 2301 464 382 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2086 4045 4357 4503 5649 2086
Size: 1959 312 146 1146 2276
Fragments arranged by size:
2276 1959 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1082 2716 2849 1082
Size: 1634 133 4072
Fragments arranged by size:
4072 1634 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 692 1538 1665 1852 2508 2581 2767 3662
Size: 511 846 127 187 656 73 186 895
Cuts at: 3662 4094 4843 5111 181
Size: 432 749 268 909
Fragments arranged by size:
909 895 846 749 656 511 432 268
187 186 127 73
EcoNI CCTnn'n_nnAGG
Cuts at: 1680 1680
Size: 5839
EcoO109I rG'GnC_Cy
Cuts at: 743 1318 1543 2976 743
Size: 575 225 1433 3606
Fragments arranged by size:
3606 1433 575 225
EcoRI G'AATT_C
Cuts at: 2841 2841
Size: 5839
EcoRV GAT'ATC
Cuts at: 732 875 732
Size: 143 5696
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1408 3856 1408
Size: 2448 3391
FspI TGC'GCA
Cuts at: 3618 3618
Size: 5839
HaeII r_GCGC'y
Cuts at: 3271 3519 4543 5522 3271
Size: 248 1024 979 3588
Fragments arranged by size:
3588 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 315 347 459 491 1071 1103 2005 2037 2134
Size: 32 112 32 580 32 902 32 97
Cuts at: 2134 2166 3679 3709 3711 3741 315
Size: 32 1513 30 2 30 2413
Fragments arranged by size:
2413 1513 902 580 112 97 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 897 2876 897
Size: 1979 3860
KasI G'GCGC_C
Cuts at: 3267 3515 3267
Size: 248 5591
KpnI G_GTAC'C
Cuts at: 2857 2857
Size: 5839
MfeI C'AATT_G
Cuts at: 2792 2792
Size: 5839
MluI A'CGCG_T
Cuts at: 5272 5272
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1622 2060 4510 4694 1030
Size: 592 438 2450 184 2175
Fragments arranged by size:
2450 2175 592 438 184
MscI TGG'CCA
Cuts at: 3598 3598
Size: 5839
MslI CAynn'nnrTG
Cuts at: 1580 2261 3826 3953 1580
Size: 681 1565 127 3466
Fragments arranged by size:
3466 1565 681 127
MspA1I CmG'CkG
Cuts at: 1293 2948 3262 3622 4637 4882 1293
Size: 1655 314 360 1015 245 2250
Fragments arranged by size:
2250 1655 1015 360 314 245
NaeI GCC'GGC
Cuts at: 4018 4018
Size: 5839
NarI GG'CG_CC
Cuts at: 3268 3516 3268
Size: 248 5591
NcoI C'CATG_G
Cuts at: 125 125
Size: 5839
NgoMIV G'CCGG_C
Cuts at: 4016 4016
Size: 5839
NruI TCG'CGA
Cuts at: 4200 4200
Size: 5839
NspI r_CATG'y
Cuts at: 1420 2890 3390 4299 1420
Size: 1470 500 909 2960
Fragments arranged by size:
2960 1470 909 500
PasI CC'CwG_GG
Cuts at: 2805 2805
Size: 5839
PciI A'CATG_T
Cuts at: 4295 4295
Size: 5839
PflMI CCAn_nnn'nTGG
Cuts at: 283 1469 1898 1996 283
Size: 1186 429 98 4126
Fragments arranged by size:
4126 1186 429 98
PfoI T'CCnGG_A
Cuts at: 158 1554 158
Size: 1396 4443
PmeI GTTT'AAAC
Cuts at: 2836 2836
Size: 5839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1763 1795 4978 5010 1763
Size: 32 3183 32 2592
Fragments arranged by size:
3183 2592 32 32
PpuMI rG'GwC_Cy
Cuts at: 743 743
Size: 5839
PshAI GACnn'nnGTC
Cuts at: 2667 2667
Size: 5839
PsiI TTA'TAA
Cuts at: 931 931
Size: 5839
PstI C_TGCA'G
Cuts at: 1317 2884 3569 1317
Size: 1567 685 3587
Fragments arranged by size:
3587 1567 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5839
PvuII CAG'CTG
Cuts at: 1293 3262 3622 1293
Size: 1969 360 3510
Fragments arranged by size:
3510 1969 360
RsrII CG'GwC_CG
Cuts at: 4032 4032
Size: 5839
SacI G_AGCT'C
Cuts at: 1084 2718 2851 1084
Size: 1634 133 4072
Fragments arranged by size:
4072 1634 133
SalI G'TCGA_C
Cuts at: 2874 2874
Size: 5839
SapI GCTCTTCn'nnn_
Cuts at: 3860 4070 3860
Size: 210 5629
SbfI CC_TGCA'GG
Cuts at: 2884 2884
Size: 5839
ScaI AGT'ACT
Cuts at: 5356 5356
Size: 5839
SexAI A'CCwGG_T
Cuts at: 1179 1179
Size: 5839
SfcI C'TryA_G
Cuts at: 33 1313 1978 2517 2880 3565 4560 4751 33
Size: 1280 665 539 363 685 995 191 1121
Fragments arranged by size:
1280 1121 995 685 665 539 363 191
SfoI GGC'GCC
Cuts at: 3269 3517 3269
Size: 248 5591
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5839
SmaI CCC'GGG
Cuts at: 2859 2859
Size: 5839
SmlI C'TyrA_G
Cuts at: 1299 2992 3907 4401 4663 1299
Size: 1693 915 494 262 2475
Fragments arranged by size:
2475 1693 915 494 262
SnaBI TAC'GTA
Cuts at: 2829 2829
Size: 5839
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5839
SphI G_CATG'C
Cuts at: 1420 2890 3390 1420
Size: 1470 500 3869
Fragments arranged by size:
3869 1470 500
SspI AAT'ATT
Cuts at: 353 1954 2167 353
Size: 1601 213 4025
Fragments arranged by size:
4025 1601 213
StuI AGG'CCT
Cuts at: 215 967 1434 215
Size: 752 467 4620
Fragments arranged by size:
4620 752 467
StyI C'CwwG_G
Cuts at: 125 432 735 2971 3325 125
Size: 307 303 2236 354 2639
Fragments arranged by size:
2639 2236 354 307 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1798 2818 3420 3553 4012 4223 1798
Size: 1020 602 133 459 211 3414
Fragments arranged by size:
3414 1020 602 459 211 133
TatI w'GTAC_w
Cuts at: 1266 5354 1266
Size: 4088 1751
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1655 1687 5356 5388 1655
Size: 32 3669 32 2106
Fragments arranged by size:
3669 2106 32 32
Tth111I GACn'n_nGTC
Cuts at: 3634 3634
Size: 5839
XbaI T'CTAG_A
Cuts at: 766 2868 766
Size: 2102 3737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2812 3332 2812
Size: 520 5319
XmaI C'CCGG_G
Cuts at: 2857 2857
Size: 5839
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsmBI BssHII BstZ17I ClaI CspCI FseI FspAI
HindIII HpaI NdeI NheI NotI NsiI PacI PmlI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SrfI
SwaI XhoI XmnI ZraI