Restriction map for
pF1KSDA0091
(Circular) (Six-base) MAPSORT of: pF1KSDA0091.seq Check: 3845 from: 1 to: 6293
pF1KSDA0091 6293 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 877 1378 877
Size: 501 5792
AccI GT'mk_AC
Cuts at: 371 3331 371
Size: 2960 3333
Acc65I G'GTAC_C
Cuts at: 3309 3309
Size: 6293
AclI AA'CG_TT
Cuts at: 6133 6133
Size: 6293
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 2258 2277 4118 4550 5297 5565 376
Size: 1882 19 1841 432 747 268 1104
Fragments arranged by size:
1882 1841 1104 747 432 268 19
AflIII A'CryG_T
Cuts at: 1031 1496 1613 2173 2477 3645 4749 5726 1031
Size: 465 117 560 304 1168 1104 977 1598
Fragments arranged by size:
1598 1168 1104 977 560 465 304 117
AgeI A'CCGG_T
Cuts at: 4681 4681
Size: 6293
AhdI GACnn_n'nnGTC
Cuts at: 1604 1604
Size: 6293
AleI CACnn'nnGTG
Cuts at: 880 880
Size: 6293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 694 694
Size: 6293
AlwNI CAG_nnn'CTG
Cuts at: 714 1393 1461 2127 2300 3598 5165 714
Size: 679 68 666 173 1298 1567 1842
Fragments arranged by size:
1842 1567 1298 679 666 173 68
ApaI G_GGCC'C
Cuts at: 1353 2166 1353
Size: 813 5480
ApaLI G'TGCA_C
Cuts at: 5063 5063
Size: 6293
ApoI r'AATT_y
Cuts at: 262 333 3297 5646 5902 262
Size: 71 2964 2349 256 653
Fragments arranged by size:
2964 2349 653 256 71
AseI AT'TA_AT
Cuts at: 20 20
Size: 6293
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6293
AvaI C'yCGr_G
Cuts at: 1486 2158 3313 1486
Size: 672 1155 4466
Fragments arranged by size:
4466 1155 672
BamHI G'GATC_C
Cuts at: 3318 3318
Size: 6293
BanI G'GyrC_C
Cuts at: 1343 2949 3067 3309 3723 3971 4006 1343
Size: 1606 118 242 414 248 35 3630
Fragments arranged by size:
3630 1606 414 248 242 118 35
BanII G_rGCy'C
Cuts at: 637 1353 1582 2166 3307 4337 637
Size: 716 229 584 1141 1030 2593
Fragments arranged by size:
2593 1141 1030 716 584 229
BbeI G_GCGC'C
Cuts at: 2953 3071 3727 3975 2953
Size: 118 656 248 5271
Fragments arranged by size:
5271 656 248 118
BbvCI CC'TCA_GC
Cuts at: 1042 2630 1042
Size: 1588 4705
BciVI GTATCCnnnnn_n'
Cuts at: 304 2364 4191 4952 6293 304
Size: 2060 1827 761 1341 304
Fragments arranged by size:
2060 1827 1341 761 304
BclI T'GATC_A
Cuts at: 1176 1877 2088 3813 1176
Size: 701 211 1725 3656
Fragments arranged by size:
3656 1725 701 211
BglI GCCn_nnn'nGGC
Cuts at: 1725 1725
Size: 6293
BglII A'GATC_T
Cuts at: 1593 3808 5845 1593
Size: 2215 2037 2041
Fragments arranged by size:
2215 2041 2037
BlpI GC'TnA_GC
Cuts at: 787 3405 787
Size: 2618 3675
Bme1580I G_kGCm'C
Cuts at: 354 1353 2166 3918 4011 5067 354
Size: 999 813 1752 93 1056 1580
Fragments arranged by size:
1752 1580 1056 999 813 93
BmgBI CAC'GTC
Cuts at: 604 1032 2265 604
Size: 428 1233 4632
Fragments arranged by size:
4632 1233 428
BmrI ACTGGGnnnn_n'
Cuts at: 791 2786 2948 3505 3586 3921 6065 791
Size: 1995 162 557 81 335 2144 1019
Fragments arranged by size:
2144 1995 1019 557 335 162 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 640 4660 640
Size: 4020 2273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 911 1361 2710 2798 2881 3077 3104 911
Size: 450 1349 88 83 196 27 4100
Fragments arranged by size:
4100 1349 450 196 88 83 27
Bpu10I CC'TnA_GC
Cuts at: 1042 1862 2630 1042
Size: 820 768 4705
Fragments arranged by size:
4705 820 768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 3020 3469 4348 4840 5138 517
Size: 2503 449 879 492 298 1672
Fragments arranged by size:
2503 1672 879 492 449 298
BsaI GGTCTCn'nnnn_
Cuts at: 2870 5997 2870
Size: 3127 3166
BsaAI yAC'GTr
Cuts at: 2174 3285 3646 2174
Size: 1111 361 4821
Fragments arranged by size:
4821 1111 361
BsaBI GATnn'nnATC
Cuts at: 6 3807 5844 6
Size: 3801 2037 455
Fragments arranged by size:
3801 2037 455
BsaHI Gr'CG_yC
Cuts at: 998 2525 2950 3068 3724 3972 4674 6170 998
Size: 1527 425 118 656 248 702 1496 1121
Fragments arranged by size:
1527 1496 1121 702 656 425 248 118
BsaWI w'CCGG_w
Cuts at: 2255 2374 3482 4003 4681 4955 5102 5513 2255
Size: 119 1108 521 678 274 147 411 3035
Fragments arranged by size:
3035 1108 678 521 411 274 147 119
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3143 3173 3143
Size: 30 6263
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1770 2236 2239 2393 2713 1770
Size: 466 3 154 320 5350
Fragments arranged by size:
5350 466 320 154 3
BseYI C'CCAG_C
Cuts at: 1582 1631 1893 2391 3718 4476 5053 5549 1582
Size: 49 262 498 1327 758 577 496 2326
Fragments arranged by size:
2326 1327 758 577 498 496 262 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1940 2517 1940
Size: 577 5716
BsiEI CG_ry'CG
Cuts at: 104 1281 3025 3835 3881 5089 104
Size: 1177 1744 810 46 1208 1308
Fragments arranged by size:
1744 1308 1208 1177 810 46
BsiHKAI G_wGCw'C
Cuts at: 731 1414 3307 4085 4275 5067 731
Size: 683 1893 778 190 792 1957
Fragments arranged by size:
1957 1893 792 778 683 190
BsmI GAATG_Cn'
Cuts at: 663 1658 663
Size: 995 5298
BsmBI CGTCTCn'nnnn_
Cuts at: 2938 2938
Size: 6293
Bsp1286I G_dGCh'C
Cuts at: 354 637 731 1353 1414 1582 2166 3307 3918
Size: 283 94 622 61 168 584 1141 611
Cuts at: 3918 4011 4085 4275 4337 5067 354
Size: 93 74 190 62 730 1580
Fragments arranged by size:
1580 1141 730 622 611 584 283 190
168 94 93 74 62 61
BspEI T'CCGG_A
Cuts at: 2255 5513 2255
Size: 3258 3035
BspHI T'CATG_A
Cuts at: 1874 5469 1874
Size: 3595 2698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 877 1378 3243 3343 3557 3859 4240 877
Size: 501 1865 100 214 302 381 2930
Fragments arranged by size:
2930 1865 501 381 302 214 100
BsrBI CCG'CTC
Cuts at: 50 3065 4585 4639 6123 6288 50
Size: 3015 1520 54 1484 165 55
Fragments arranged by size:
3015 1520 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 573 1143 1856 4205 573
Size: 570 713 2349 2661
Fragments arranged by size:
2661 2349 713 570
BsrFI r'CCGG_y
Cuts at: 4291 4472 4681 4291
Size: 181 209 5903
Fragments arranged by size:
5903 209 181
BssSI C'ACGA_G
Cuts at: 157 4564 4922 157
Size: 4407 358 1528
Fragments arranged by size:
4407 1528 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3246 3670 3246
Size: 424 5869
BstBI TT'CG_AA
Cuts at: 110 4654 110
Size: 4544 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 2673 2859 2994 2673
Size: 186 135 5972
Fragments arranged by size:
5972 186 135
BstYI r'GATC_y
Cuts at: 1 1544 1593 3318 3785 3808 4143 4389 5401
Size: 1543 49 1725 467 23 335 246 1012
Cuts at: 5401 5487 5499 5845 1
Size: 86 12 346 449
Fragments arranged by size:
1725 1543 1012 467 449 346 335 246
86 49 23 12
BtgI C'CryG_G
Cuts at: 870 1512 3155 870
Size: 642 1643 4008
Fragments arranged by size:
4008 1643 642
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1209 2450 2487 4243 4424 4587 1209
Size: 1241 37 1756 181 163 2915
Fragments arranged by size:
2915 1756 1241 181 163 37
BtsI GCAGTG_nn'
Cuts at: 369 712 2362 3536 369
Size: 343 1650 1174 3126
Fragments arranged by size:
3126 1650 1174 343
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 260 295 260
Size: 35 6258
DraI TTT'AAA
Cuts at: 1019 2570 2939 3292 1019
Size: 1551 369 353 4020
Fragments arranged by size:
4020 1551 369 353
DrdI GACnn_nn'nnGTC
Cuts at: 3999 4857 3999
Size: 858 5435
EaeI y'GGCC_r
Cuts at: 1811 2465 3099 3878 4052 4443 4470 5815 1811
Size: 654 634 779 174 391 27 1345 2289
Fragments arranged by size:
2289 1345 779 654 634 391 174 27
EagI C'GGCC_G
Cuts at: 3878 3878
Size: 6293
EarI CTCTTCn'nnn_
Cuts at: 352 609 2234 4316 4526 352
Size: 257 1625 2082 210 2119
Fragments arranged by size:
2119 2082 1625 257 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4501 4811 4957 6103 4501
Size: 310 146 1146 4691
Fragments arranged by size:
4691 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3305 3305
Size: 6293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 911 1361 2258 2277 2710 2798 2881 3077
Size: 535 450 897 19 433 88 83 196
Cuts at: 3077 3104 4118 4550 5297 5565 376
Size: 27 1014 432 747 268 1104
Fragments arranged by size:
1104 1014 897 747 535 450 433 432
268 196 88 83 27 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2998 2998
Size: 6293
EcoO109I rG'GnC_Cy
Cuts at: 222 1350 1458 2154 2162 2163 3044 3432 222
Size: 1128 108 696 8 1 881 388 3083
Fragments arranged by size:
3083 1128 881 696 388 108 8 1
EcoRI G'AATT_C
Cuts at: 262 3297 262
Size: 3035 3258
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2718 4312 2718
Size: 1594 4699
FspI TGC'GCA
Cuts at: 4074 4074
Size: 6293
HaeII r_GCGC'y
Cuts at: 2787 2953 3071 3237 3727 3975 4997 5976 2787
Size: 166 118 166 490 248 1022 979 3104
Fragments arranged by size:
3104 1022 979 490 248 166 166 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1590 1622 1891 1923 2372 2395 2404 2427 4135
Size: 32 269 32 449 23 9 23 1708
Cuts at: 4135 4165 4167 4197 1590
Size: 30 2 30 3686
Fragments arranged by size:
3686 1708 449 269 32 32 30 30
23 23 9 2
HincII GTy'rAC
Cuts at: 138 1083 1494 3332 138
Size: 945 411 1838 3099
Fragments arranged by size:
3099 1838 945 411
HindIII A'AGCT_T
Cuts at: 130 130
Size: 6293
KasI G'GCGC_C
Cuts at: 2949 3067 3723 3971 2949
Size: 118 656 248 5271
Fragments arranged by size:
5271 656 248 118
KpnI G_GTAC'C
Cuts at: 3313 3313
Size: 6293
MfeI C'AATT_G
Cuts at: 375 2667 375
Size: 2292 4001
MluI A'CGCG_T
Cuts at: 5726 5726
Size: 6293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4964 5148 4964
Size: 184 6109
MscI TGG'CCA
Cuts at: 1813 2467 4054 1813
Size: 654 1587 4052
Fragments arranged by size:
4052 1587 654
MslI CAynn'nnrTG
Cuts at: 880 1825 1954 4282 4409 880
Size: 945 129 2328 127 2764
Fragments arranged by size:
2764 2328 945 129 127
MspA1I CmG'CkG
Cuts at: 2629 3404 3718 4078 5091 5336 2629
Size: 775 314 360 1013 245 3586
Fragments arranged by size:
3586 1013 775 360 314 245
NaeI GCC'GGC
Cuts at: 4474 4474
Size: 6293
NarI GG'CG_CC
Cuts at: 2950 3068 3724 3972 2950
Size: 118 656 248 5271
Fragments arranged by size:
5271 656 248 118
NcoI C'CATG_G
Cuts at: 870 3155 870
Size: 2285 4008
NgoMIV G'CCGG_C
Cuts at: 4472 4472
Size: 6293
NruI TCG'CGA
Cuts at: 2506 2506
Size: 6293
NspI r_CATG'y
Cuts at: 652 1500 1617 2481 3346 3846 4753 652
Size: 848 117 864 865 500 907 2192
Fragments arranged by size:
2192 907 865 864 848 500 117
PasI CC'CwG_GG
Cuts at: 630 2042 3224 630
Size: 1412 1182 3699
Fragments arranged by size:
3699 1412 1182
PciI A'CATG_T
Cuts at: 1496 1613 2477 4749 1496
Size: 117 864 2272 3040
Fragments arranged by size:
3040 2272 864 117
PflMI CCAn_nnn'nTGG
Cuts at: 232 2521 2559 3117 232
Size: 2289 38 558 3408
Fragments arranged by size:
3408 2289 558 38
PfoI T'CCnGG_A
Cuts at: 3081 3081
Size: 6293
PmeI GTTT'AAAC
Cuts at: 3292 3292
Size: 6293
PmlI CAC'GTG
Cuts at: 2174 2174
Size: 6293
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5432 5464 5432
Size: 32 6261
PpuMI rG'GwC_Cy
Cuts at: 2154 2154
Size: 6293
PspOMI G'GGCC_C
Cuts at: 1349 2162 1349
Size: 813 5480
PstI C_TGCA'G
Cuts at: 719 3340 4025 719
Size: 2621 685 2987
Fragments arranged by size:
2987 2621 685
PvuI CG_AT'CG
Cuts at: 104 3025 104
Size: 2921 3372
PvuII CAG'CTG
Cuts at: 2629 3718 4078 2629
Size: 1089 360 4844
Fragments arranged by size:
4844 1089 360
RsrII CG'GwC_CG
Cuts at: 4488 4488
Size: 6293
SacI G_AGCT'C
Cuts at: 3307 3307
Size: 6293
SalI G'TCGA_C
Cuts at: 3330 3330
Size: 6293
SapI GCTCTTCn'nnn_
Cuts at: 352 609 4316 4526 352
Size: 257 3707 210 2119
Fragments arranged by size:
3707 2119 257 210
SbfI CC_TGCA'GG
Cuts at: 3340 3340
Size: 6293
ScaI AGT'ACT
Cuts at: 2230 5810 2230
Size: 3580 2713
SfcI C'TryA_G
Cuts at: 33 715 3336 4021 5014 5205 33
Size: 682 2621 685 993 191 1121
Fragments arranged by size:
2621 1121 993 685 682 191
SfoI GGC'GCC
Cuts at: 2951 3069 3725 3973 2951
Size: 118 656 248 5271
Fragments arranged by size:
5271 656 248 118
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6293
SmaI CCC'GGG
Cuts at: 1488 3315 1488
Size: 1827 4466
SmlI C'TyrA_G
Cuts at: 532 3035 3448 4363 4855 5117 532
Size: 2503 413 915 492 262 1708
Fragments arranged by size:
2503 1708 915 492 413 262
SnaBI TAC'GTA
Cuts at: 3285 3285
Size: 6293
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6293
SphI G_CATG'C
Cuts at: 652 3346 3846 652
Size: 2694 500 3099
Fragments arranged by size:
3099 2694 500
StyI C'CwwG_G
Cuts at: 870 915 1795 2553 2852 3155 3427 3781 870
Size: 45 880 758 299 303 272 354 3382
Fragments arranged by size:
3382 880 758 354 303 299 272 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1773 3876 4009 4468 4677 1773
Size: 2103 133 459 209 3389
Fragments arranged by size:
3389 2103 459 209 133
TatI w'GTAC_w
Cuts at: 2228 2649 2729 5808 2228
Size: 421 80 3079 2713
Fragments arranged by size:
3079 2713 421 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3143 3175 5810 5842 3143
Size: 32 2635 32 3594
Fragments arranged by size:
3594 2635 32 32
Tth111I GACn'n_nGTC
Cuts at: 1297 1905 4090 1297
Size: 608 2185 3500
Fragments arranged by size:
3500 2185 608
XbaI T'CTAG_A
Cuts at: 3324 3324
Size: 6293
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 627 2915 3162 3788 627
Size: 2288 247 626 3132
Fragments arranged by size:
3132 2288 626 247
XmaI C'CCGG_G
Cuts at: 1486 3313 1486
Size: 1827 4466
XmnI GAAnn'nnTTC
Cuts at: 663 663
Size: 6293
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BaeI BbsI
BcgI BfrBI BmtI BsiWI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoRV FseI FspAI HpaI NdeI
NheI NotI NsiI PacI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI ZraI