Restriction map for
pF1KSDA0086
(Circular) (Six-base) MAPSORT of: pF1KSDA0086.seq Check: 7184 from: 1 to: 6257
pF1KSDA0086 6257 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 708 1368 1722 708
Size: 660 354 5243
Fragments arranged by size:
5243 660 354
AccI GT'mk_AC
Cuts at: 3295 3295
Size: 6257
Acc65I G'GTAC_C
Cuts at: 3273 3273
Size: 6257
AclI AA'CG_TT
Cuts at: 2595 6097 2595
Size: 3502 2755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 987 1201 2129 2556 3166 4082 4514 5261
Size: 115 214 928 427 610 916 432 747
Cuts at: 5261 5529 872
Size: 268 1600
Fragments arranged by size:
1600 928 916 747 610 432 427 268
214 115
AfeI AGC'GCT
Cuts at: 3130 3130
Size: 6257
AflII C'TTAA_G
Cuts at: 1750 1750
Size: 6257
AflIII A'CryG_T
Cuts at: 450 452 2224 2894 3609 4713 5690 450
Size: 2 1772 670 715 1104 977 1017
Fragments arranged by size:
1772 1104 1017 977 715 670 2
AgeI A'CCGG_T
Cuts at: 4645 4645
Size: 6257
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1656 1656
Size: 6257
AlwNI CAG_nnn'CTG
Cuts at: 1010 2107 2515 3144 3562 5129 1010
Size: 1097 408 629 418 1567 2138
Fragments arranged by size:
2138 1567 1097 629 418 408
ApaLI G'TGCA_C
Cuts at: 5027 5027
Size: 6257
ApoI r'AATT_y
Cuts at: 366 978 1426 1957 2049 3089 3261 5610 5866
Size: 612 448 531 92 1040 172 2349 256
Cuts at: 5866 366
Size: 757
Fragments arranged by size:
2349 1040 757 612 531 448 256 172
92
AseI AT'TA_AT
Cuts at: 20 2096 2873 2936 20
Size: 2076 777 63 3341
Fragments arranged by size:
3341 2076 777 63
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6257
AvaI C'yCGr_G
Cuts at: 1226 3277 1226
Size: 2051 4206
BamHI G'GATC_C
Cuts at: 3282 3282
Size: 6257
BanI G'GyrC_C
Cuts at: 3181 3273 3687 3935 3970 3181
Size: 92 414 248 35 5468
Fragments arranged by size:
5468 414 248 92 35
BanII G_rGCy'C
Cuts at: 3271 4301 3271
Size: 1030 5227
BbeI G_GCGC'C
Cuts at: 3691 3939 3691
Size: 248 6009
BbsI GAAGACnn'nnnn_
Cuts at: 140 155 592 758 1052 2130 2775 140
Size: 15 437 166 294 1078 645 3622
Fragments arranged by size:
3622 1078 645 437 294 166 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 517 551 517
Size: 34 6223
BciVI GTATCCnnnnn_n'
Cuts at: 478 4155 4916 6257 478
Size: 3677 761 1341 478
Fragments arranged by size:
3677 1341 761 478
BclI T'GATC_A
Cuts at: 675 2331 3777 675
Size: 1656 1446 3155
Fragments arranged by size:
3155 1656 1446
BglI GCCn_nnn'nGGC
Cuts at: 2716 2716
Size: 6257
BglII A'GATC_T
Cuts at: 3772 5809 3772
Size: 2037 4220
BlpI GC'TnA_GC
Cuts at: 3369 3369
Size: 6257
Bme1580I G_kGCm'C
Cuts at: 323 3184 3882 3975 5031 323
Size: 2861 698 93 1056 1549
Fragments arranged by size:
2861 1549 1056 698 93
BmrI ACTGGGnnnn_n'
Cuts at: 3469 3550 3885 6029 3469
Size: 81 335 2144 3697
Fragments arranged by size:
3697 2144 335 81
BmtI G_CTAG'C
Cuts at: 658 658
Size: 6257
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 659 4624 659
Size: 3965 2292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 401
Size: 6257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 3433 4312 4804 5102 772
Size: 2661 879 492 298 1927
Fragments arranged by size:
2661 1927 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5961 5961
Size: 6257
BsaAI yAC'GTr
Cuts at: 453 3249 3610 453
Size: 2796 361 3100
Fragments arranged by size:
3100 2796 361
BsaBI GATnn'nnATC
Cuts at: 6 2498 3771 5808 6
Size: 2492 1273 2037 455
Fragments arranged by size:
2492 2037 1273 455
BsaHI Gr'CG_yC
Cuts at: 737 3688 3936 4638 6134 737
Size: 2951 248 702 1496 860
Fragments arranged by size:
2951 1496 860 702 248
BsaWI w'CCGG_w
Cuts at: 1071 2690 3446 3967 4645 4919 5066 5477 1071
Size: 1619 756 521 678 274 147 411 1851
Fragments arranged by size:
1851 1619 756 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1701 1731 1701
Size: 30 6227
BseYI C'CCAG_C
Cuts at: 2584 3682 4440 5017 5513 2584
Size: 1098 758 577 496 3328
Fragments arranged by size:
3328 1098 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2919 2919
Size: 6257
BsiEI CG_ry'CG
Cuts at: 104 3799 3845 5053 104
Size: 3695 46 1208 1308
Fragments arranged by size:
3695 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3202 3271 4049 4239 5031 3202
Size: 69 778 190 792 4428
Fragments arranged by size:
4428 792 778 190 69
BsmI GAATG_Cn'
Cuts at: 1614 1614
Size: 6257
BsmBI CGTCTCn'nnnn_
Cuts at: 848 848
Size: 6257
Bsp1286I G_dGCh'C
Cuts at: 323 3184 3202 3271 3882 3975 4049 4239 4301
Size: 2861 18 69 611 93 74 190 62
Cuts at: 4301 5031 323
Size: 730 1549
Fragments arranged by size:
2861 1549 730 611 190 93 74 69
62 18
BspEI T'CCGG_A
Cuts at: 5477 5477
Size: 6257
BspHI T'CATG_A
Cuts at: 1053 2519 5433 1053
Size: 1466 2914 1877
Fragments arranged by size:
2914 1877 1466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 325 708 1368 1722 3307 3521 3823 4204 325
Size: 383 660 354 1585 214 302 381 2378
Fragments arranged by size:
2378 1585 660 383 381 354 302 214
BsrBI CCG'CTC
Cuts at: 50 4549 4603 6087 6252 50
Size: 4499 54 1484 165 55
Fragments arranged by size:
4499 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 985 2440 2792 4169 985
Size: 1455 352 1377 3073
Fragments arranged by size:
3073 1455 1377 352
BsrFI r'CCGG_y
Cuts at: 2254 4255 4436 4645 2254
Size: 2001 181 209 3866
Fragments arranged by size:
3866 2001 209 181
BssSI C'ACGA_G
Cuts at: 4528 4886 4528
Size: 358 5899
BstAPI GCAn_nnn'nTGC
Cuts at: 3634 3634
Size: 6257
BstBI TT'CG_AA
Cuts at: 110 4618 110
Size: 4508 1749
BstYI r'GATC_y
Cuts at: 1 2580 3282 3749 3772 4107 4353 5365 5451
Size: 2579 702 467 23 335 246 1012 86
Cuts at: 5451 5463 5809 1
Size: 12 346 449
Fragments arranged by size:
2579 1012 702 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4207 4388 4551 4207
Size: 181 163 5913
Fragments arranged by size:
5913 181 163
BtsI GCAGTG_nn'
Cuts at: 1924 2053 2705 3500 1924
Size: 129 652 795 4681
Fragments arranged by size:
4681 795 652 129
ClaI AT'CG_AT
Cuts at: 1061 1061
Size: 6257
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1346 1381 1346
Size: 35 6222
DraI TTT'AAA
Cuts at: 831 1158 1688 3256 831
Size: 327 530 1568 3832
Fragments arranged by size:
3832 1568 530 327
DrdI GACnn_nn'nnGTC
Cuts at: 2617 3963 4821 2617
Size: 1346 858 4053
Fragments arranged by size:
4053 1346 858
EaeI y'GGCC_r
Cuts at: 3842 4016 4407 4434 5779 3842
Size: 174 391 27 1345 4320
Fragments arranged by size:
4320 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3842 3842
Size: 6257
EarI CTCTTCn'nnn_
Cuts at: 1502 1832 2071 2606 4280 4490 1502
Size: 330 239 535 1674 210 3269
Fragments arranged by size:
3269 1674 535 330 239 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4465 4775 4921 6067 4465
Size: 310 146 1146 4655
Fragments arranged by size:
4655 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3269 3269
Size: 6257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 872 987 1201 2129 2556 3166 4082 4514
Size: 471 115 214 928 427 610 916 432
Cuts at: 4514 5261 5529 401
Size: 747 268 1129
Fragments arranged by size:
1129 928 916 747 610 471 432 427
268 214 115
EcoNI CCTnn'n_nnAGG
Cuts at: 704 704
Size: 6257
EcoO109I rG'GnC_Cy
Cuts at: 1910 3396 1910
Size: 1486 4771
EcoRI G'AATT_C
Cuts at: 366 3089 3261 366
Size: 2723 172 3362
Fragments arranged by size:
3362 2723 172
EcoRV GAT'ATC
Cuts at: 1059 1059
Size: 6257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 340 782 1513 1570 1787 4276 340
Size: 442 731 57 217 2489 2321
Fragments arranged by size:
2489 2321 731 442 217 57
FspI TGC'GCA
Cuts at: 4038 4038
Size: 6257
HaeII r_GCGC'y
Cuts at: 3132 3691 3939 4961 5940 3132
Size: 559 248 1022 979 3449
Fragments arranged by size:
3449 1022 979 559 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 715 747 858 890 2070 2102 4099 4129 4131
Size: 32 111 32 1180 32 1997 30 2
Cuts at: 4131 4161 715
Size: 30 2811
Fragments arranged by size:
2811 1997 1180 111 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3296 3296
Size: 6257
HindIII A'AGCT_T
Cuts at: 1375 2410 1375
Size: 1035 5222
KasI G'GCGC_C
Cuts at: 3687 3935 3687
Size: 248 6009
KpnI G_GTAC'C
Cuts at: 3277 3277
Size: 6257
MluI A'CGCG_T
Cuts at: 5690 5690
Size: 6257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4928 5112 4928
Size: 184 6073
MscI TGG'CCA
Cuts at: 4018 4018
Size: 6257
MslI CAynn'nnrTG
Cuts at: 537 4246 4373 537
Size: 3709 127 2421
Fragments arranged by size:
3709 2421 127
MspA1I CmG'CkG
Cuts at: 1145 3368 3682 4042 5055 5300 1145
Size: 2223 314 360 1013 245 2102
Fragments arranged by size:
2223 2102 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4438 4438
Size: 6257
NarI GG'CG_CC
Cuts at: 3688 3936 3688
Size: 248 6009
NdeI CA'TA_TG
Cuts at: 2624 2624
Size: 6257
NgoMIV G'CCGG_C
Cuts at: 4436 4436
Size: 6257
NheI G'CTAG_C
Cuts at: 654 654
Size: 6257
NspI r_CATG'y
Cuts at: 547 1331 1637 2228 2740 2826 2898 3310 3810
Size: 784 306 591 512 86 72 412 500
Cuts at: 3810 4717 547
Size: 907 2087
Fragments arranged by size:
2087 907 784 591 512 500 412 306
86 72
PciI A'CATG_T
Cuts at: 2224 2894 4713 2224
Size: 670 1819 3768
Fragments arranged by size:
3768 1819 670
PflMI CCAn_nnn'nTGG
Cuts at: 202 202
Size: 6257
PfoI T'CCnGG_A
Cuts at: 417 417
Size: 6257
PmeI GTTT'AAAC
Cuts at: 3256 3256
Size: 6257
PmlI CAC'GTG
Cuts at: 453 453
Size: 6257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5396 5428 5396
Size: 32 6225
PpuMI rG'GwC_Cy
Cuts at: 1910 1910
Size: 6257
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2244 2276 2244
Size: 32 6225
PstI C_TGCA'G
Cuts at: 1645 2060 3304 3989 1645
Size: 415 1244 685 3913
Fragments arranged by size:
3913 1244 685 415
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6257
PvuII CAG'CTG
Cuts at: 1145 3682 4042 1145
Size: 2537 360 3360
Fragments arranged by size:
3360 2537 360
RsrII CG'GwC_CG
Cuts at: 4452 4452
Size: 6257
SacI G_AGCT'C
Cuts at: 3271 3271
Size: 6257
SalI G'TCGA_C
Cuts at: 3294 3294
Size: 6257
SapI GCTCTTCn'nnn_
Cuts at: 4280 4490 4280
Size: 210 6047
SbfI CC_TGCA'GG
Cuts at: 3304 3304
Size: 6257
ScaI AGT'ACT
Cuts at: 1308 1971 5774 1308
Size: 663 3803 1791
Fragments arranged by size:
3803 1791 663
SfcI C'TryA_G
Cuts at: 33 429 1641 2056 2848 3008 3300 3985 4978
Size: 396 1212 415 792 160 292 685 993
Cuts at: 4978 5169 33
Size: 191 1121
Fragments arranged by size:
1212 1121 993 792 685 415 396 292
191 160
SfoI GGC'GCC
Cuts at: 3689 3937 3689
Size: 248 6009
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6257
SmaI CCC'GGG
Cuts at: 3279 3279
Size: 6257
SmlI C'TyrA_G
Cuts at: 751 1750 3412 4327 4819 5081 751
Size: 999 1662 915 492 262 1927
Fragments arranged by size:
1927 1662 999 915 492 262
SnaBI TAC'GTA
Cuts at: 3249 3249
Size: 6257
SpeI A'CTAG_T
Cuts at: 67 933 67
Size: 866 5391
SphI G_CATG'C
Cuts at: 3310 3810 3310
Size: 500 5757
SspI AAT'ATT
Cuts at: 216 1736 3214 216
Size: 1520 1478 3259
Fragments arranged by size:
3259 1520 1478
StyI C'CwwG_G
Cuts at: 324 1778 3391 3745 324
Size: 1454 1613 354 2836
Fragments arranged by size:
2836 1613 1454 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2453 3840 3973 4432 4641 2453
Size: 1387 133 459 209 4069
Fragments arranged by size:
4069 1387 459 209 133
TatI w'GTAC_w
Cuts at: 1306 1330 1708 1969 2630 5772 1306
Size: 24 378 261 661 3142 1791
Fragments arranged by size:
3142 1791 661 378 261 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2053 2085 5774 5806 2053
Size: 32 3689 32 2504
Fragments arranged by size:
3689 2504 32 32
Tth111I GACn'n_nGTC
Cuts at: 729 1169 4054 729
Size: 440 2885 2932
Fragments arranged by size:
2932 2885 440
XbaI T'CTAG_A
Cuts at: 3288 3288
Size: 6257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3752 3752
Size: 6257
XmaI C'CCGG_G
Cuts at: 3277 3277
Size: 6257
XmnI GAAnn'nnTTC
Cuts at: 1216 2782 3075 1216
Size: 1566 293 4398
Fragments arranged by size:
4398 1566 293
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NheI NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AleI AloI ApaI AscI AvrII BaeI
BbvCI BfrBI BmgBI Bpu10I BseRI BsiWI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI DraIII FseI FspAI
HpaI MfeI NcoI NotI NruI NsiI PacI PasI
PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SrfI StuI SwaI XhoI ZraI