Restriction map for
pF1KSDA0081
(Circular) (Six-base) MAPSORT of: pF1KSDA0081.seq Check: 952 from: 1 to: 3839
pF1KSDA0081 3839 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AatII G_ACGT'C
Cuts at: 566 566
Size: 3839
AccI GT'mk_AC
Cuts at: 256 398 877 256
Size: 142 479 3218
Fragments arranged by size:
3218 479 142
Acc65I G'GTAC_C
Cuts at: 855 855
Size: 3839
AclI AA'CG_TT
Cuts at: 3679 3679
Size: 3839
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 1664 2096 2843 3111 371
Size: 1293 432 747 268 1099
Fragments arranged by size:
1293 1099 747 432 268
AflIII A'CryG_T
Cuts at: 1191 2295 3272 1191
Size: 1104 977 1758
Fragments arranged by size:
1758 1104 977
AgeI A'CCGG_T
Cuts at: 2227 2227
Size: 3839
AlwNI CAG_nnn'CTG
Cuts at: 394 1144 2711 394
Size: 750 1567 1522
Fragments arranged by size:
1567 1522 750
ApaLI G'TGCA_C
Cuts at: 2609 2609
Size: 3839
ApoI r'AATT_y
Cuts at: 843 3192 3448 843
Size: 2349 256 1234
Fragments arranged by size:
2349 1234 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3839
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3839
AvaI C'yCGr_G
Cuts at: 238 859 238
Size: 621 3218
BamHI G'GATC_C
Cuts at: 864 864
Size: 3839
BanI G'GyrC_C
Cuts at: 855 1269 1517 1552 855
Size: 414 248 35 3142
Fragments arranged by size:
3142 414 248 35
BanII G_rGCy'C
Cuts at: 853 1883 853
Size: 1030 2809
BbeI G_GCGC'C
Cuts at: 1273 1521 1273
Size: 248 3591
BbsI GAAGACnn'nnnn_
Cuts at: 313 466 776 827 313
Size: 153 310 51 3325
Fragments arranged by size:
3325 310 153 51
BciVI GTATCCnnnnn_n'
Cuts at: 1737 2498 3839 1737
Size: 761 1341 1737
Fragments arranged by size:
1737 1341 761
BclI T'GATC_A
Cuts at: 1359 1359
Size: 3839
BglII A'GATC_T
Cuts at: 363 768 1354 3391 363
Size: 405 586 2037 811
Fragments arranged by size:
2037 811 586 405
BlpI GC'TnA_GC
Cuts at: 951 951
Size: 3839
Bme1580I G_kGCm'C
Cuts at: 1464 1557 2613 1464
Size: 93 1056 2690
Fragments arranged by size:
2690 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1051 1132 1467 3611 1051
Size: 81 335 2144 1279
Fragments arranged by size:
2144 1279 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 3839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 700 346
Size: 354 3485
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1894 2386 2684 1015
Size: 879 492 298 2170
Fragments arranged by size:
2170 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 375 3543 375
Size: 3168 671
BsaAI yAC'GTr
Cuts at: 831 1192 831
Size: 361 3478
BsaBI GATnn'nnATC
Cuts at: 6 1353 3390 6
Size: 1347 2037 455
Fragments arranged by size:
2037 1347 455
BsaHI Gr'CG_yC
Cuts at: 244 563 1270 1518 2220 3716 244
Size: 319 707 248 702 1496 367
Fragments arranged by size:
1496 707 702 367 319 248
BsaWI w'CCGG_w
Cuts at: 1028 1549 2227 2501 2648 3059 1028
Size: 521 678 274 147 411 1808
Fragments arranged by size:
1808 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 249 279 249
Size: 30 3809
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 722 722
Size: 3839
BseYI C'CCAG_C
Cuts at: 805 1264 2022 2599 3095 805
Size: 459 758 577 496 1549
Fragments arranged by size:
1549 758 577 496 459
BsiEI CG_ry'CG
Cuts at: 104 227 1381 1427 2635 104
Size: 123 1154 46 1208 1308
Fragments arranged by size:
1308 1208 1154 123 46
BsiHKAI G_wGCw'C
Cuts at: 377 853 1631 1821 2613 377
Size: 476 778 190 792 1603
Fragments arranged by size:
1603 792 778 476 190
Bsp1286I G_dGCh'C
Cuts at: 377 853 1464 1557 1631 1821 1883 2613 377
Size: 476 611 93 74 190 62 730 1603
Fragments arranged by size:
1603 730 611 476 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3059 3059
Size: 3839
BspHI T'CATG_A
Cuts at: 467 3015 467
Size: 2548 1291
BspMI ACCTGCnnnn'nnnn_
Cuts at: 188 889 1103 1405 1786 188
Size: 701 214 302 381 2241
Fragments arranged by size:
2241 701 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2131 2185 3669 3834 50
Size: 2081 54 1484 165 55
Fragments arranged by size:
2081 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 337 1751 337
Size: 1414 2425
BsrFI r'CCGG_y
Cuts at: 1837 2018 2227 1837
Size: 181 209 3449
Fragments arranged by size:
3449 209 181
BssHII G'CGCG_C
Cuts at: 145 145
Size: 3839
BssSI C'ACGA_G
Cuts at: 2110 2468 2110
Size: 358 3481
BstAPI GCAn_nnn'nTGC
Cuts at: 1216 1216
Size: 3839
BstBI TT'CG_AA
Cuts at: 110 2200 110
Size: 2090 1749
BstYI r'GATC_y
Cuts at: 1 363 768 864 1331 1354 1689 1935 2947
Size: 362 405 96 467 23 335 246 1012
Cuts at: 2947 3033 3045 3391 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 405 362 346 335 246
96 86 23 12
BtgI C'CryG_G
Cuts at: 125 155 125
Size: 30 3809
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 626 1789 1970 2133 626
Size: 1163 181 163 2332
Fragments arranged by size:
2332 1163 181 163
BtsI GCAGTG_nn'
Cuts at: 1082 1082
Size: 3839
DraI TTT'AAA
Cuts at: 838 838
Size: 3839
DrdI GACnn_nn'nnGTC
Cuts at: 646 1545 2403 646
Size: 899 858 2082
Fragments arranged by size:
2082 899 858
EaeI y'GGCC_r
Cuts at: 224 1424 1598 1989 2016 3361 224
Size: 1200 174 391 27 1345 702
Fragments arranged by size:
1345 1200 702 391 174 27
EagI C'GGCC_G
Cuts at: 224 1424 224
Size: 1200 2639
EarI CTCTTCn'nnn_
Cuts at: 1862 2072 1862
Size: 210 3629
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2047 2357 2503 3649 2047
Size: 310 146 1146 2237
Fragments arranged by size:
2237 1146 310 146
EcoICRI GAG'CTC
Cuts at: 851 851
Size: 3839
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 371 700 1664 2096 2843 3111 346
Size: 25 329 964 432 747 268 1074
Fragments arranged by size:
1074 964 747 432 329 268 25
EcoNI CCTnn'n_nnAGG
Cuts at: 532 532
Size: 3839
EcoO109I rG'GnC_Cy
Cuts at: 215 978 215
Size: 763 3076
EcoRI G'AATT_C
Cuts at: 843 843
Size: 3839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 382 1858 382
Size: 1476 2363
FspI TGC'GCA
Cuts at: 1620 1620
Size: 3839
HaeII r_GCGC'y
Cuts at: 1273 1521 2543 3522 1273
Size: 248 1022 979 1590
Fragments arranged by size:
1590 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 760 792 1681 1711 1713 1743 760
Size: 32 889 30 2 30 2856
Fragments arranged by size:
2856 889 32 30 30 2
HincII GTy'rAC
Cuts at: 399 878 399
Size: 479 3360
KasI G'GCGC_C
Cuts at: 1269 1517 1269
Size: 248 3591
KpnI G_GTAC'C
Cuts at: 859 859
Size: 3839
MluI A'CGCG_T
Cuts at: 3272 3272
Size: 3839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2510 2694 2510
Size: 184 3655
MscI TGG'CCA
Cuts at: 1600 1600
Size: 3839
MslI CAynn'nnrTG
Cuts at: 1828 1955 1828
Size: 127 3712
MspA1I CmG'CkG
Cuts at: 950 1264 1624 2637 2882 950
Size: 314 360 1013 245 1907
Fragments arranged by size:
1907 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2020 2020
Size: 3839
NarI GG'CG_CC
Cuts at: 1270 1518 1270
Size: 248 3591
NcoI C'CATG_G
Cuts at: 125 125
Size: 3839
NgoMIV G'CCGG_C
Cuts at: 2018 2018
Size: 3839
NruI TCG'CGA
Cuts at: 293 611 293
Size: 318 3521
NspI r_CATG'y
Cuts at: 892 1392 2299 892
Size: 500 907 2432
Fragments arranged by size:
2432 907 500
PciI A'CATG_T
Cuts at: 2295 2295
Size: 3839
PflMI CCAn_nnn'nTGG
Cuts at: 211 211
Size: 3839
PmeI GTTT'AAAC
Cuts at: 838 838
Size: 3839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2978 3010 2978
Size: 32 3807
PpuMI rG'GwC_Cy
Cuts at: 215 215
Size: 3839
PstI C_TGCA'G
Cuts at: 886 1571 886
Size: 685 3154
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3839
PvuII CAG'CTG
Cuts at: 1264 1624 1264
Size: 360 3479
RsrII CG'GwC_CG
Cuts at: 2034 2034
Size: 3839
SacI G_AGCT'C
Cuts at: 853 853
Size: 3839
SalI G'TCGA_C
Cuts at: 397 876 397
Size: 479 3360
SapI GCTCTTCn'nnn_
Cuts at: 1862 2072 1862
Size: 210 3629
SbfI CC_TGCA'GG
Cuts at: 886 886
Size: 3839
ScaI AGT'ACT
Cuts at: 3356 3356
Size: 3839
SfcI C'TryA_G
Cuts at: 33 882 1567 2560 2751 33
Size: 849 685 993 191 1121
Fragments arranged by size:
1121 993 849 685 191
SfoI GGC'GCC
Cuts at: 1271 1519 1271
Size: 248 3591
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3839
SmaI CCC'GGG
Cuts at: 240 861 240
Size: 621 3218
SmlI C'TyrA_G
Cuts at: 994 1909 2401 2663 994
Size: 915 492 262 2170
Fragments arranged by size:
2170 915 492 262
SnaBI TAC'GTA
Cuts at: 831 831
Size: 3839
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3839
SphI G_CATG'C
Cuts at: 892 1392 892
Size: 500 3339
StyI C'CwwG_G
Cuts at: 125 788 973 1327 125
Size: 663 185 354 2637
Fragments arranged by size:
2637 663 354 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 640 770 1422 1555 2014 2223 640
Size: 130 652 133 459 209 2256
Fragments arranged by size:
2256 652 459 209 133 130
TatI w'GTAC_w
Cuts at: 3354 3354
Size: 3839
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 3807
Tth111I GACn'n_nGTC
Cuts at: 1636 1636
Size: 3839
XbaI T'CTAG_A
Cuts at: 870 870
Size: 3839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1334 1334
Size: 3839
XmaI C'CCGG_G
Cuts at: 238 859 238
Size: 621 3218
ZraI GAC'GTC
Cuts at: 564 564
Size: 3839
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
StuI SwaI XhoI XmnI