Restriction map for
pF1KSDA0080
(Circular) (Six-base) MAPSORT of: pF1KSDA0080.seq Check: 2866 from: 1 to: 4432
pF1KSDA0080 4432 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1133 1468 1133
Size: 335 4097
Acc65I G'GTAC_C
Cuts at: 1446 1446
Size: 4432
AclI AA'CG_TT
Cuts at: 4272 4272
Size: 4432
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2255 2687 3436 3704 2255
Size: 432 749 268 2983
Fragments arranged by size:
2983 749 432 268
AflIII A'CryG_T
Cuts at: 1782 2888 3865 1782
Size: 1106 977 2349
Fragments arranged by size:
2349 1106 977
AgeI A'CCGG_T
Cuts at: 168 1096 2820 168
Size: 928 1724 1780
Fragments arranged by size:
1780 1724 928
AlwNI CAG_nnn'CTG
Cuts at: 427 843 1735 3304 427
Size: 416 892 1569 1555
Fragments arranged by size:
1569 1555 892 416
ApaLI G'TGCA_C
Cuts at: 1182 3202 1182
Size: 2020 2412
ApoI r'AATT_y
Cuts at: 1434 3785 4041 1434
Size: 2351 256 1825
Fragments arranged by size:
2351 1825 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4432
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4432
AvaI C'yCGr_G
Cuts at: 884 1450 884
Size: 566 3866
BamHI G'GATC_C
Cuts at: 601 1455 601
Size: 854 3578
BanI G'GyrC_C
Cuts at: 912 1446 1860 2108 2143 912
Size: 534 414 248 35 3201
Fragments arranged by size:
3201 534 414 248 35
BanII G_rGCy'C
Cuts at: 529 1010 1378 1444 2474 529
Size: 481 368 66 1030 2487
Fragments arranged by size:
2487 1030 481 368 66
BbeI G_GCGC'C
Cuts at: 1864 2112 1864
Size: 248 4184
BbsI GAAGACnn'nnnn_
Cuts at: 763 781 763
Size: 18 4414
BbvCI CC'TCA_GC
Cuts at: 331 865 331
Size: 534 3898
BciVI GTATCCnnnnn_n'
Cuts at: 2328 3091 4432 2328
Size: 763 1341 2328
Fragments arranged by size:
2328 1341 763
BclI T'GATC_A
Cuts at: 1950 1950
Size: 4432
BglII A'GATC_T
Cuts at: 1945 3984 1945
Size: 2039 2393
BlpI GC'TnA_GC
Cuts at: 436 1542 436
Size: 1106 3326
Bme1580I G_kGCm'C
Cuts at: 1186 1326 2055 2148 3206 1186
Size: 140 729 93 1058 2412
Fragments arranged by size:
2412 1058 729 140 93
BmrI ACTGGGnnnn_n'
Cuts at: 1642 1723 2058 4204 1642
Size: 81 335 2146 1870
Fragments arranged by size:
2146 1870 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1024 2797 1024
Size: 1773 2659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 994 1377 563
Size: 431 383 3618
Fragments arranged by size:
3618 431 383
Bpu10I CC'TnA_GC
Cuts at: 331 865 331
Size: 534 3898
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1606 2485 2979 3277 1606
Size: 879 494 298 2761
Fragments arranged by size:
2761 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 319 793 1105 4136 319
Size: 474 312 3031 615
Fragments arranged by size:
3031 615 474 312
BsaAI yAC'GTr
Cuts at: 1422 1783 1422
Size: 361 4071
BsaBI GATnn'nnATC
Cuts at: 6 1090 1944 3983 6
Size: 1084 854 2039 455
Fragments arranged by size:
2039 1084 854 455
BsaHI Gr'CG_yC
Cuts at: 1861 2109 2813 4309 1861
Size: 248 704 1496 1984
Fragments arranged by size:
1984 1496 704 248
BsaWI w'CCGG_w
Cuts at: 168 1096 1619 2140 2820 3094 3241 3652 168
Size: 928 523 521 680 274 147 411 948
Fragments arranged by size:
948 928 680 523 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 375 405 552 582 375
Size: 30 147 30 4225
Fragments arranged by size:
4225 147 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 598 598
Size: 4432
BseYI C'CCAG_C
Cuts at: 934 1855 2613 3192 3688 934
Size: 921 758 579 496 1678
Fragments arranged by size:
1678 921 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 835
Size: 4432
BsiEI CG_ry'CG
Cuts at: 104 1281 1972 2018 3228 104
Size: 1177 691 46 1210 1308
Fragments arranged by size:
1308 1210 1177 691 46
BsiHKAI G_wGCw'C
Cuts at: 1010 1186 1444 2222 2412 3206 1010
Size: 176 258 778 190 794 2236
Fragments arranged by size:
2236 794 778 258 190 176
Bsp1286I G_dGCh'C
Cuts at: 529 1010 1186 1326 1378 1444 2055 2148 2222
Size: 481 176 140 52 66 611 93 74
Cuts at: 2222 2412 2474 3206 529
Size: 190 62 732 1755
Fragments arranged by size:
1755 732 611 481 190 176 140 93
74 66 62 52
BspEI T'CCGG_A
Cuts at: 3652 3652
Size: 4432
BspHI T'CATG_A
Cuts at: 3608 3608
Size: 4432
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1480 1694 1996 2377 1480
Size: 214 302 381 3535
Fragments arranged by size:
3535 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2722 2776 4262 4427 50
Size: 2672 54 1486 165 55
Fragments arranged by size:
2672 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1151 2342 1151
Size: 1191 3241
BsrFI r'CCGG_y
Cuts at: 168 178 1096 2428 2609 2820 168
Size: 10 918 1332 181 211 1780
Fragments arranged by size:
1780 1332 918 211 181 10
BssSI C'ACGA_G
Cuts at: 2701 3061 2701
Size: 360 4072
BstAPI GCAn_nnn'nTGC
Cuts at: 1807 1807
Size: 4432
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4432
BstEII G'GTnAC_C
Cuts at: 219 219
Size: 4432
BstXI CCAn_nnnn'nTGG
Cuts at: 501 501
Size: 4432
BstYI r'GATC_y
Cuts at: 1 450 472 601 703 1455 1922 1945 2280
Size: 449 22 129 102 752 467 23 335
Cuts at: 2280 2526 3540 3626 3638 3984 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 752 467 449 449 346 335 246
129 102 86 23 22 12
BtgI C'CryG_G
Cuts at: 125 669 125
Size: 544 3888
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2380 2561 2724 2380
Size: 181 163 4088
Fragments arranged by size:
4088 181 163
BtsI GCAGTG_nn'
Cuts at: 1673 1673
Size: 4432
DraI TTT'AAA
Cuts at: 1429 1429
Size: 4432
DraIII CAC_nnn'GTG
Cuts at: 1330 1330
Size: 4432
DrdI GACnn_nn'nnGTC
Cuts at: 2136 2996 2136
Size: 860 3572
EaeI y'GGCC_r
Cuts at: 176 1391 2015 2189 2580 2607 3954 176
Size: 1215 624 174 391 27 1347 654
Fragments arranged by size:
1347 1215 654 624 391 174 27
EagI C'GGCC_G
Cuts at: 2015 2015
Size: 4432
EarI CTCTTCn'nnn_
Cuts at: 2453 2663 2453
Size: 210 4222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2638 2950 3096 4242 2638
Size: 312 146 1146 2828
Fragments arranged by size:
2828 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1008 1442 1008
Size: 434 3998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 994 1377 2255 2687 3436 3704 563
Size: 431 383 878 432 749 268 1291
Fragments arranged by size:
1291 878 749 432 431 383 268
EcoO109I rG'GnC_Cy
Cuts at: 191 460 840 1569 191
Size: 269 380 729 3054
Fragments arranged by size:
3054 729 380 269
EcoRI G'AATT_C
Cuts at: 1434 1434
Size: 4432
EcoRV GAT'ATC
Cuts at: 1092 1248 1092
Size: 156 4276
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 4432
FseI GG_CCGG'CC
Cuts at: 182 182
Size: 4432
FspI TGC'GCA
Cuts at: 2211 2211
Size: 4432
HaeII r_GCGC'y
Cuts at: 1864 2112 3136 4115 1864
Size: 248 1024 979 2181
Fragments arranged by size:
2181 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 865 897 2272 2302 2304 2334 865
Size: 32 1375 30 2 30 2963
Fragments arranged by size:
2963 1375 32 30 30 2
HincII GTy'rAC
Cuts at: 1469 1469
Size: 4432
KasI G'GCGC_C
Cuts at: 1860 2108 1860
Size: 248 4184
KpnI G_GTAC'C
Cuts at: 1450 1450
Size: 4432
MluI A'CGCG_T
Cuts at: 3865 3865
Size: 4432
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 171 3103 3287 171
Size: 2932 184 1316
Fragments arranged by size:
2932 1316 184
MscI TGG'CCA
Cuts at: 1393 2191 1393
Size: 798 3634
MslI CAynn'nnrTG
Cuts at: 355 499 721 2419 2546 355
Size: 144 222 1698 127 2241
Fragments arranged by size:
2241 1698 222 144 127
MspA1I CmG'CkG
Cuts at: 834 1541 1855 2215 3230 3475 834
Size: 707 314 360 1015 245 1791
Fragments arranged by size:
1791 1015 707 360 314 245
NaeI GCC'GGC
Cuts at: 180 2611 180
Size: 2431 2001
NarI GG'CG_CC
Cuts at: 1861 2109 1861
Size: 248 4184
NcoI C'CATG_G
Cuts at: 125 125
Size: 4432
NgoMIV G'CCGG_C
Cuts at: 178 2609 178
Size: 2431 2001
NruI TCG'CGA
Cuts at: 2793 2793
Size: 4432
NspI r_CATG'y
Cuts at: 300 1483 1983 2892 300
Size: 1183 500 909 1840
Fragments arranged by size:
1840 1183 909 500
PasI CC'CwG_GG
Cuts at: 455 547 699 455
Size: 92 152 4188
Fragments arranged by size:
4188 152 92
PciI A'CATG_T
Cuts at: 2888 2888
Size: 4432
PfoI T'CCnGG_A
Cuts at: 659 659
Size: 4432
PmeI GTTT'AAAC
Cuts at: 1429 1429
Size: 4432
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3571 3603 3571
Size: 32 4400
PpuMI rG'GwC_Cy
Cuts at: 840 840
Size: 4432
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 265 297 1120 1152 265
Size: 32 823 32 3545
Fragments arranged by size:
3545 823 32 32
PstI C_TGCA'G
Cuts at: 839 1477 2162 839
Size: 638 685 3109
Fragments arranged by size:
3109 685 638
PvuI CG_AT'CG
Cuts at: 104 1281 104
Size: 1177 3255
PvuII CAG'CTG
Cuts at: 834 1855 2215 834
Size: 1021 360 3051
Fragments arranged by size:
3051 1021 360
RsrII CG'GwC_CG
Cuts at: 2625 2625
Size: 4432
SacI G_AGCT'C
Cuts at: 1010 1444 1010
Size: 434 3998
SalI G'TCGA_C
Cuts at: 1467 1467
Size: 4432
SapI GCTCTTCn'nnn_
Cuts at: 2453 2663 2453
Size: 210 4222
SbfI CC_TGCA'GG
Cuts at: 1477 1477
Size: 4432
ScaI AGT'ACT
Cuts at: 1417 3949 1417
Size: 2532 1900
SexAI A'CCwGG_T
Cuts at: 842 842
Size: 4432
SfcI C'TryA_G
Cuts at: 33 825 835 1473 2158 3153 3344 33
Size: 792 10 638 685 995 191 1121
Fragments arranged by size:
1121 995 792 685 638 191 10
SfoI GGC'GCC
Cuts at: 1862 2110 1862
Size: 248 4184
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4432
SgrAI Cr'CCGG_yG
Cuts at: 168 168
Size: 4432
SmaI CCC'GGG
Cuts at: 1452 1452
Size: 4432
SmlI C'TyrA_G
Cuts at: 1585 2500 2994 3256 1585
Size: 915 494 262 2761
Fragments arranged by size:
2761 915 494 262
SnaBI TAC'GTA
Cuts at: 1422 1422
Size: 4432
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4432
SphI G_CATG'C
Cuts at: 1483 1983 1483
Size: 500 3932
StyI C'CwwG_G
Cuts at: 125 1564 1918 125
Size: 1439 354 2639
Fragments arranged by size:
2639 1439 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2013 2146 2605 2816 2013
Size: 133 459 211 3629
Fragments arranged by size:
3629 459 211 133
TatI w'GTAC_w
Cuts at: 1415 3947 1415
Size: 2532 1900
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 375 407 3949 3981 375
Size: 32 3542 32 826
Fragments arranged by size:
3542 826 32 32
Tth111I GACn'n_nGTC
Cuts at: 2227 2227
Size: 4432
XbaI T'CTAG_A
Cuts at: 1461 1461
Size: 4432
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1296 1925 1296
Size: 629 3803
XmaI C'CCGG_G
Cuts at: 1450 1450
Size: 4432
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PfoI PmeI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI EcoNI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PflMI PmlI PshAI PsiI
PspOMI PspXI SacII SanDI SfiI SrfI SspI StuI
SwaI XhoI XmnI ZraI