Restriction map for
pF1KSDA0053
(Circular) (Six-base) MAPSORT of: pF1KSDA0053.seq Check: 4898 from: 1 to: 5051
pF1KSDA0053 5051 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 2089 2089
Size: 5051
Acc65I G'GTAC_C
Cuts at: 2067 2067
Size: 5051
AclI AA'CG_TT
Cuts at: 4891 4891
Size: 5051
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1275 1280 2876 3308 4055 4323 283
Size: 992 5 1596 432 747 268 1011
Fragments arranged by size:
1596 1011 992 747 432 268 5
AfeI AGC'GCT
Cuts at: 583 1948 583
Size: 1365 3686
AflIII A'CryG_T
Cuts at: 2403 3507 4484 2403
Size: 1104 977 2970
Fragments arranged by size:
2970 1104 977
AgeI A'CCGG_T
Cuts at: 3439 3439
Size: 5051
AleI CACnn'nnGTG
Cuts at: 784 784
Size: 5051
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 218 218
Size: 5051
AlwNI CAG_nnn'CTG
Cuts at: 2356 3923 2356
Size: 1567 3484
ApaLI G'TGCA_C
Cuts at: 787 1655 3821 787
Size: 868 2166 2017
Fragments arranged by size:
2166 2017 868
ApoI r'AATT_y
Cuts at: 535 613 1271 1507 2002 2055 4404 4660 535
Size: 78 658 236 495 53 2349 256 926
Fragments arranged by size:
2349 926 658 495 256 236 78 53
AseI AT'TA_AT
Cuts at: 20 20
Size: 5051
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5051
AvaI C'yCGr_G
Cuts at: 1952 2071 1952
Size: 119 4932
BamHI G'GATC_C
Cuts at: 2076 2076
Size: 5051
BanI G'GyrC_C
Cuts at: 630 1471 2067 2481 2729 2764 630
Size: 841 596 414 248 35 2917
Fragments arranged by size:
2917 841 596 414 248 35
BanII G_rGCy'C
Cuts at: 839 2065 3095 839
Size: 1226 1030 2795
Fragments arranged by size:
2795 1226 1030
BbeI G_GCGC'C
Cuts at: 2485 2733 2485
Size: 248 4803
BbsI GAAGACnn'nnnn_
Cuts at: 1088 1268 1088
Size: 180 4871
BbvCI CC'TCA_GC
Cuts at: 313 313
Size: 5051
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1069 1103 1069
Size: 34 5017
BciVI GTATCCnnnnn_n'
Cuts at: 2949 3710 5051 2949
Size: 761 1341 2949
Fragments arranged by size:
2949 1341 761
BclI T'GATC_A
Cuts at: 1094 1139 2571 1094
Size: 45 1432 3574
Fragments arranged by size:
3574 1432 45
BglII A'GATC_T
Cuts at: 1493 2566 4603 1493
Size: 1073 2037 1941
Fragments arranged by size:
2037 1941 1073
BlpI GC'TnA_GC
Cuts at: 911 2163 911
Size: 1252 3799
Bme1580I G_kGCm'C
Cuts at: 635 791 1550 1659 2676 2769 3825 635
Size: 156 759 109 1017 93 1056 1861
Fragments arranged by size:
1861 1056 1017 759 156 109 93
BmrI ACTGGGnnnn_n'
Cuts at: 1727 2263 2344 2679 4823 1727
Size: 536 81 335 2144 1955
Fragments arranged by size:
2144 1955 536 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 780 1124 3418 780
Size: 344 2294 2413
Fragments arranged by size:
2413 2294 344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 688 870 1208 1760 1912 256
Size: 432 182 338 552 152 3395
Fragments arranged by size:
3395 552 432 338 182 152
Bpu10I CC'TnA_GC
Cuts at: 313 967 1532 313
Size: 654 565 3832
Fragments arranged by size:
3832 654 565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1550 1858 2227 3106 3598 3896 1550
Size: 308 369 879 492 298 2705
Fragments arranged by size:
2705 879 492 369 308 298
BsaI GGTCTCn'nnnn_
Cuts at: 818 1139 4755 818
Size: 321 3616 1114
Fragments arranged by size:
3616 1114 321
BsaAI yAC'GTr
Cuts at: 2043 2404 2043
Size: 361 4690
BsaBI GATnn'nnATC
Cuts at: 6 2565 4602 6
Size: 2559 2037 455
Fragments arranged by size:
2559 2037 455
BsaHI Gr'CG_yC
Cuts at: 2482 2730 3432 4928 2482
Size: 248 702 1496 2605
Fragments arranged by size:
2605 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1330 1356 1439 2240 2761 3439 3713 3860 4271
Size: 26 83 801 521 678 274 147 411
Cuts at: 4271 1330
Size: 2110
Fragments arranged by size:
2110 801 678 521 411 274 147 83
26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 538 1100 538
Size: 562 4489
BseYI C'CCAG_C
Cuts at: 424 751 1247 2476 3234 3811 4307 424
Size: 327 496 1229 758 577 496 1168
Fragments arranged by size:
1229 1168 758 577 496 496 327
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 674
Size: 5051
BsiEI CG_ry'CG
Cuts at: 104 678 2593 2639 3847 104
Size: 574 1915 46 1208 1308
Fragments arranged by size:
1915 1308 1208 574 46
BsiHKAI G_wGCw'C
Cuts at: 674 791 1659 2065 2843 3033 3825 674
Size: 117 868 406 778 190 792 1900
Fragments arranged by size:
1900 868 792 778 406 190 117
BsmI GAATG_Cn'
Cuts at: 897 897
Size: 5051
BsmBI CGTCTCn'nnnn_
Cuts at: 731 731
Size: 5051
Bsp1286I G_dGCh'C
Cuts at: 635 674 791 839 1550 1659 2065 2676 2769
Size: 39 117 48 711 109 406 611 93
Cuts at: 2769 2843 3033 3095 3825 635
Size: 74 190 62 730 1861
Fragments arranged by size:
1861 730 711 611 406 190 117 109
93 74 62 48 39
BspEI T'CCGG_A
Cuts at: 1356 4271 1356
Size: 2915 2136
BspHI T'CATG_A
Cuts at: 1097 4227 1097
Size: 3130 1921
BspMI ACCTGCnnnn'nnnn_
Cuts at: 971 2101 2315 2617 2998 971
Size: 1130 214 302 381 3024
Fragments arranged by size:
3024 1130 381 302 214
BsrBI CCG'CTC
Cuts at: 50 759 3343 3397 4881 5046 50
Size: 709 2584 54 1484 165 55
Fragments arranged by size:
2584 1484 709 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2963 2963
Size: 5051
BsrFI r'CCGG_y
Cuts at: 3049 3230 3439 3049
Size: 181 209 4661
Fragments arranged by size:
4661 209 181
BsrGI T'GTAC_A
Cuts at: 388 388
Size: 5051
BssSI C'ACGA_G
Cuts at: 947 3322 3680 947
Size: 2375 358 2318
Fragments arranged by size:
2375 2318 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2428 2428
Size: 5051
BstBI TT'CG_AA
Cuts at: 110 3412 110
Size: 3302 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 234 252 458 642 1397 234
Size: 18 206 184 755 3888
Fragments arranged by size:
3888 755 206 184 18
BstYI r'GATC_y
Cuts at: 1 1115 1493 2076 2543 2566 2901 3147 4159
Size: 1114 378 583 467 23 335 246 1012
Cuts at: 4159 4245 4257 4603 1
Size: 86 12 346 449
Fragments arranged by size:
1114 1012 583 467 449 378 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 541 541
Size: 5051
BtgI C'CryG_G
Cuts at: 839 839
Size: 5051
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1361 3001 3182 3345 1361
Size: 1640 181 163 3067
Fragments arranged by size:
3067 1640 181 163
BtsI GCAGTG_nn'
Cuts at: 2294 2294
Size: 5051
DraI TTT'AAA
Cuts at: 1500 2050 1500
Size: 550 4501
DraIII CAC_nnn'GTG
Cuts at: 565 795 565
Size: 230 4821
DrdI GACnn_nn'nnGTC
Cuts at: 490 1153 2757 3615 490
Size: 663 1604 858 1926
Fragments arranged by size:
1926 1604 858 663
EaeI y'GGCC_r
Cuts at: 500 576 675 2636 2810 3201 3228 4573 500
Size: 76 99 1961 174 391 27 1345 978
Fragments arranged by size:
1961 1345 978 391 174 99 76 27
EagI C'GGCC_G
Cuts at: 675 2636 675
Size: 1961 3090
EarI CTCTTCn'nnn_
Cuts at: 341 680 1165 1668 1739 1963 3074 3284 341
Size: 339 485 503 71 224 1111 210 2108
Fragments arranged by size:
2108 1111 503 485 339 224 210 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 529 3259 3569 3715 4861 529
Size: 2730 310 146 1146 719
Fragments arranged by size:
2730 1146 719 310 146
EcoICRI GAG'CTC
Cuts at: 2063 2063
Size: 5051
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 283 688 870 1208 1275 1280 1760 1912
Size: 27 405 182 338 67 5 480 152
Cuts at: 1912 2876 3308 4055 4323 256
Size: 964 432 747 268 984
Fragments arranged by size:
984 964 747 480 432 405 338 268
182 152 67 27 5
EcoNI CCTnn'n_nnAGG
Cuts at: 1840 1840
Size: 5051
EcoO109I rG'GnC_Cy
Cuts at: 1634 2190 1634
Size: 556 4495
EcoRI G'AATT_C
Cuts at: 1507 2055 1507
Size: 548 4503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 453 3070 453
Size: 2617 2434
FspI TGC'GCA
Cuts at: 2832 2832
Size: 5051
HaeII r_GCGC'y
Cuts at: 585 1950 2485 2733 3755 4734 585
Size: 1365 535 248 1022 979 902
Fragments arranged by size:
1365 1022 979 902 535 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 377 409 2893 2923 2925 2955 377
Size: 32 2484 30 2 30 2473
Fragments arranged by size:
2484 2473 32 30 30 2
HincII GTy'rAC
Cuts at: 1017 2090 1017
Size: 1073 3978
HpaI GTT'AAC
Cuts at: 1017 1017
Size: 5051
KasI G'GCGC_C
Cuts at: 2481 2729 2481
Size: 248 4803
KpnI G_GTAC'C
Cuts at: 2071 2071
Size: 5051
MluI A'CGCG_T
Cuts at: 4484 4484
Size: 5051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3722 3906 3722
Size: 184 4867
MscI TGG'CCA
Cuts at: 502 578 2812 502
Size: 76 2234 2741
Fragments arranged by size:
2741 2234 76
MslI CAynn'nnrTG
Cuts at: 250 640 784 3040 3167 250
Size: 390 144 2256 127 2134
Fragments arranged by size:
2256 2134 390 144 127
MspA1I CmG'CkG
Cuts at: 234 732 1002 2162 2476 2836 3849 4094 234
Size: 498 270 1160 314 360 1013 245 1191
Fragments arranged by size:
1191 1160 1013 498 360 314 270 245
NaeI GCC'GGC
Cuts at: 3232 3232
Size: 5051
NarI GG'CG_CC
Cuts at: 2482 2730 2482
Size: 248 4803
NgoMIV G'CCGG_C
Cuts at: 3230 3230
Size: 5051
NspI r_CATG'y
Cuts at: 375 2104 2604 3511 375
Size: 1729 500 907 1915
Fragments arranged by size:
1915 1729 907 500
PasI CC'CwG_GG
Cuts at: 363 1379 1639 363
Size: 1016 260 3775
Fragments arranged by size:
3775 1016 260
PciI A'CATG_T
Cuts at: 3507 3507
Size: 5051
PflMI CCAn_nnn'nTGG
Cuts at: 585 585
Size: 5051
PfoI T'CCnGG_A
Cuts at: 665 665
Size: 5051
PmeI GTTT'AAAC
Cuts at: 2050 2050
Size: 5051
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1548 1580 4190 4222 1548
Size: 32 2610 32 2377
Fragments arranged by size:
2610 2377 32 32
PpuMI rG'GwC_Cy
Cuts at: 1634 1634
Size: 5051
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 850 882 850
Size: 32 5019
PstI C_TGCA'G
Cuts at: 982 1917 2098 2783 982
Size: 935 181 685 3250
Fragments arranged by size:
3250 935 685 181
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5051
PvuII CAG'CTG
Cuts at: 732 1002 2476 2836 732
Size: 270 1474 360 2947
Fragments arranged by size:
2947 1474 360 270
RsrII CG'GwC_CG
Cuts at: 3246 3246
Size: 5051
SacI G_AGCT'C
Cuts at: 2065 2065
Size: 5051
SalI G'TCGA_C
Cuts at: 2088 2088
Size: 5051
SanDI GG'GwC_CC
Cuts at: 1634 1634
Size: 5051
SapI GCTCTTCn'nnn_
Cuts at: 3074 3284 3074
Size: 210 4841
SbfI CC_TGCA'GG
Cuts at: 2098 2098
Size: 5051
ScaI AGT'ACT
Cuts at: 4568 4568
Size: 5051
SexAI A'CCwGG_T
Cuts at: 719 719
Size: 5051
SfcI C'TryA_G
Cuts at: 33 161 957 978 1241 1913 2094 2779 3772
Size: 128 796 21 263 672 181 685 993
Cuts at: 3772 3963 33
Size: 191 1121
Fragments arranged by size:
1121 993 796 685 672 263 191 181
128 21
SfoI GGC'GCC
Cuts at: 2483 2731 2483
Size: 248 4803
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5051
SmaI CCC'GGG
Cuts at: 1954 2073 1954
Size: 119 4932
SmlI C'TyrA_G
Cuts at: 1565 1837 2206 3121 3613 3875 1565
Size: 272 369 915 492 262 2741
Fragments arranged by size:
2741 915 492 369 272 262
SnaBI TAC'GTA
Cuts at: 2043 2043
Size: 5051
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5051
SphI G_CATG'C
Cuts at: 2104 2604 2104
Size: 500 4551
StuI AGG'CCT
Cuts at: 1981 1981
Size: 5051
StyI C'CwwG_G
Cuts at: 1172 1682 1982 2185 2539 1172
Size: 510 300 203 354 3684
Fragments arranged by size:
3684 510 354 300 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 124 158 2047 2634 2767 3226 3435 124
Size: 34 1889 587 133 459 209 1740
Fragments arranged by size:
1889 1740 587 459 209 133 34
TatI w'GTAC_w
Cuts at: 388 4566 388
Size: 4178 873
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4568 4600 4568
Size: 32 5019
Tth111I GACn'n_nGTC
Cuts at: 2848 2848
Size: 5051
XbaI T'CTAG_A
Cuts at: 2082 2082
Size: 5051
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2546 2546
Size: 5051
XmaI C'CCGG_G
Cuts at: 1952 2071 1952
Size: 119 4932
XmnI GAAnn'nnTTC
Cuts at: 446 1447 446
Size: 1001 4050
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BsaXI BsiWI BssHII
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI SacII SfiI SgrAI
SrfI SspI SwaI XhoI ZraI