Restriction map for
pF1KSDA0037
(Circular) (Six-base) MAPSORT of: pF1KSDA0037.seq Check: 8859 from: 1 to: 6377
pF1KSDA0037 6377 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 489 1194 2302 489
Size: 705 1108 4564
Fragments arranged by size:
4564 1108 705
AccI GT'mk_AC
Cuts at: 3415 3415
Size: 6377
Acc65I G'GTAC_C
Cuts at: 1583 2735 3393 1583
Size: 1152 658 4567
Fragments arranged by size:
4567 1152 658
AclI AA'CG_TT
Cuts at: 6217 6217
Size: 6377
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 534 1573 2530 2905 4202 4634 5381 5649
Size: 264 1039 957 375 1297 432 747 268
Cuts at: 5649 270
Size: 998
Fragments arranged by size:
1297 1039 998 957 747 432 375 268
264
AflIII A'CryG_T
Cuts at: 1184 1304 1319 3729 4833 5810 1184
Size: 120 15 2410 1104 977 1751
Fragments arranged by size:
2410 1751 1104 977 120 15
AgeI A'CCGG_T
Cuts at: 4765 4765
Size: 6377
AhdI GACnn_n'nnGTC
Cuts at: 1308 1308
Size: 6377
AleI CACnn'nnGTG
Cuts at: 1222 3008 1222
Size: 1786 4591
AlwNI CAG_nnn'CTG
Cuts at: 666 1007 2121 2985 3354 3682 5249 666
Size: 341 1114 864 369 328 1567 1794
Fragments arranged by size:
1794 1567 1114 864 369 341 328
ApaI G_GGCC'C
Cuts at: 172 1905 3138 172
Size: 1733 1233 3411
Fragments arranged by size:
3411 1733 1233
ApaLI G'TGCA_C
Cuts at: 1366 5147 1366
Size: 3781 2596
ApoI r'AATT_y
Cuts at: 3381 5730 5986 3381
Size: 2349 256 3772
Fragments arranged by size:
3772 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6377
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6377
AvaI C'yCGr_G
Cuts at: 740 1254 1588 1898 2058 2075 3256 3397 740
Size: 514 334 310 160 17 1181 141 3720
Fragments arranged by size:
3720 1181 514 334 310 160 141 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3252 3285 3252
Size: 33 6344
BamHI G'GATC_C
Cuts at: 3402 3402
Size: 6377
BanI G'GyrC_C
Cuts at: 501 706 947 1583 1887 2470 2482 2735 3393
Size: 205 241 636 304 583 12 253 658
Cuts at: 3393 3807 4055 4090 501
Size: 414 248 35 2788
Fragments arranged by size:
2788 658 636 583 414 304 253 248
241 205 35 12
BanII G_rGCy'C
Cuts at: 172 1046 1793 1905 2268 2279 2879 2954 3138
Size: 874 747 112 363 11 600 75 184
Cuts at: 3138 3391 4421 172
Size: 253 1030 2128
Fragments arranged by size:
2128 1030 874 747 600 363 253 184
112 75 11
BbeI G_GCGC'C
Cuts at: 710 3811 4059 710
Size: 3101 248 3028
Fragments arranged by size:
3101 3028 248
BbsI GAAGACnn'nnnn_
Cuts at: 516 2322 2518 516
Size: 1806 196 4375
Fragments arranged by size:
4375 1806 196
BbvCI CC'TCA_GC
Cuts at: 2125 3310 2125
Size: 1185 5192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1932 1966 1932
Size: 34 6343
BciVI GTATCCnnnnn_n'
Cuts at: 4275 5036 6377 4275
Size: 761 1341 4275
Fragments arranged by size:
4275 1341 761
BclI T'GATC_A
Cuts at: 3284 3897 3284
Size: 613 5764
BglI GCCn_nnn'nGGC
Cuts at: 265 577 2383 2695 265
Size: 312 1806 312 3947
Fragments arranged by size:
3947 1806 312 312
BglII A'GATC_T
Cuts at: 3892 5929 3892
Size: 2037 4340
BlpI GC'TnA_GC
Cuts at: 1792 2953 3489 1792
Size: 1161 536 4680
Fragments arranged by size:
4680 1161 536
Bme1580I G_kGCm'C
Cuts at: 172 506 1370 1427 1905 2972 3138 4002 4095
Size: 334 864 57 478 1067 166 864 93
Cuts at: 4095 5151 172
Size: 1056 1398
Fragments arranged by size:
1398 1067 1056 864 864 478 334 166
93 57
BmgBI CAC'GTC
Cuts at: 1305 1320 1662 1305
Size: 15 342 6020
Fragments arranged by size:
6020 342 15
BmrI ACTGGGnnnn_n'
Cuts at: 635 1996 2029 3589 3670 4005 6149 635
Size: 1361 33 1560 81 335 2144 863
Fragments arranged by size:
2144 1560 1361 863 335 81 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1770 4744 1770
Size: 2974 3403
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 1876 2368 2550 2692 2851 3007 3143 3221
Size: 1588 492 182 142 159 156 136 78
Cuts at: 3221 3232 288
Size: 11 3433
Fragments arranged by size:
3433 1588 492 182 159 156 142 136
78 11
Bpu10I CC'TnA_GC
Cuts at: 403 2125 3310 403
Size: 1722 1185 3470
Fragments arranged by size:
3470 1722 1185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 1435 3553 4432 4924 5222 868
Size: 567 2118 879 492 298 2023
Fragments arranged by size:
2118 2023 879 567 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1648 2635 3226 6081 1648
Size: 987 591 2855 1944
Fragments arranged by size:
2855 1944 987 591
BsaAI yAC'GTr
Cuts at: 2694 2933 3369 3730 2694
Size: 239 436 361 5341
Fragments arranged by size:
5341 436 361 239
BsaBI GATnn'nnATC
Cuts at: 6 1462 3891 5928 6
Size: 1456 2429 2037 455
Fragments arranged by size:
2429 2037 1456 455
BsaHI Gr'CG_yC
Cuts at: 707 1564 3808 4056 4758 6254 707
Size: 857 2244 248 702 1496 830
Fragments arranged by size:
2244 1496 857 830 702 248
BsaWI w'CCGG_w
Cuts at: 1334 3566 4087 4765 5039 5186 5597 1334
Size: 2232 521 678 274 147 411 2114
Fragments arranged by size:
2232 2114 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3052 3082 3052
Size: 30 6347
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 2647 3244 178
Size: 2469 597 3311
Fragments arranged by size:
3311 2469 597
BseYI C'CCAG_C
Cuts at: 444 1513 1694 2031 2070 3354 3802 4560 5137
Size: 1069 181 337 39 1284 448 758 577
Cuts at: 5137 5633 444
Size: 496 1188
Fragments arranged by size:
1284 1188 1069 758 577 496 448 337
181 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2073 3222 2073
Size: 1149 5228
BsiEI CG_ry'CG
Cuts at: 104 1362 1744 3919 3965 5173 104
Size: 1258 382 2175 46 1208 1308
Fragments arranged by size:
2175 1308 1258 1208 382 46
BsiHKAI G_wGCw'C
Cuts at: 611 1046 1370 2399 2630 2879 3391 4169 4359
Size: 435 324 1029 231 249 512 778 190
Cuts at: 4359 5151 611
Size: 792 1837
Fragments arranged by size:
1837 1029 792 778 512 435 324 249
231 190
BsiWI C'GTAC_G
Cuts at: 1406 1757 1406
Size: 351 6026
BsmI GAATG_Cn'
Cuts at: 3277 3277
Size: 6377
BsmBI CGTCTCn'nnnn_
Cuts at: 588 588
Size: 6377
Bsp1286I G_dGCh'C
Cuts at: 172 506 611 1046 1370 1427 1793 1905 2268
Size: 334 105 435 324 57 366 112 363
Cuts at: 2268 2279 2399 2630 2879 2954 2972 3138 3391
Size: 11 120 231 249 75 18 166 253
Cuts at: 3391 4002 4095 4169 4359 4421 5151 172
Size: 611 93 74 190 62 730 1398
Fragments arranged by size:
1398 730 611 435 366 363 334 324
253 249 231 190 166 120 112 105
93 75 74 62 57 18 11
BspEI T'CCGG_A
Cuts at: 5597 5597
Size: 6377
BspHI T'CATG_A
Cuts at: 5553 5553
Size: 6377
BspMI ACCTGCnnnn'nnnn_
Cuts at: 489 827 1194 2192 2302 3244 3427 3641 3943
Size: 338 367 998 110 942 183 214 302
Cuts at: 3943 4324 489
Size: 381 2542
Fragments arranged by size:
2542 998 942 381 367 338 302 214
183 110
BsrBI CCG'CTC
Cuts at: 50 2991 4669 4723 6207 6372 50
Size: 2941 1678 54 1484 165 55
Fragments arranged by size:
2941 1678 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 278 2232 2858 4289 278
Size: 1954 626 1431 2366
Fragments arranged by size:
2366 1954 1431 626
BsrFI r'CCGG_y
Cuts at: 4375 4556 4765 4375
Size: 181 209 5987
Fragments arranged by size:
5987 209 181
BsrGI T'GTAC_A
Cuts at: 533 533
Size: 6377
BssSI C'ACGA_G
Cuts at: 1800 2629 2971 4648 5006 1800
Size: 829 342 1677 358 3171
Fragments arranged by size:
3171 1677 829 358 342
BstAPI GCAn_nnn'nTGC
Cuts at: 726 3754 726
Size: 3028 3349
BstBI TT'CG_AA
Cuts at: 110 4738 110
Size: 4628 1749
BstEII G'GTnAC_C
Cuts at: 895 3222 895
Size: 2327 4050
BstXI CCAn_nnnn'nTGG
Cuts at: 1023 1224 1338 1854 3189 1023
Size: 201 114 516 1335 4211
Fragments arranged by size:
4211 1335 516 201 114
BstYI r'GATC_y
Cuts at: 1 776 1097 3402 3869 3892 4227 4473 5485
Size: 775 321 2305 467 23 335 246 1012
Cuts at: 5485 5571 5583 5929 1
Size: 86 12 346 449
Fragments arranged by size:
2305 1012 775 467 449 346 335 321
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2335 2964 2335
Size: 629 5748
BtgI C'CryG_G
Cuts at: 851 1217 2645 3105 3276 851
Size: 366 1428 460 171 3952
Fragments arranged by size:
3952 1428 460 366 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2301 2825 3015 4327 4508 4671 2301
Size: 524 190 1312 181 163 4007
Fragments arranged by size:
4007 1312 524 190 181 163
BtsI GCAGTG_nn'
Cuts at: 254 587 1653 2428 3335 3620 254
Size: 333 1066 775 907 285 3011
Fragments arranged by size:
3011 1066 907 775 333 285
DraI TTT'AAA
Cuts at: 3376 3376
Size: 6377
DraIII CAC_nnn'GTG
Cuts at: 643 1320 1990 2807 643
Size: 677 670 817 4213
Fragments arranged by size:
4213 817 677 670
DrdI GACnn_nn'nnGTC
Cuts at: 4083 4941 4083
Size: 858 5519
EaeI y'GGCC_r
Cuts at: 248 668 866 998 1328 1359 2138 2171 2243
Size: 420 198 132 330 31 779 33 72
Cuts at: 2243 2436 3179 3962 4136 4527 4554 5899 248
Size: 193 743 783 174 391 27 1345 726
Fragments arranged by size:
1345 783 779 743 726 420 391 330
198 193 174 132 72 33 31 27
EagI C'GGCC_G
Cuts at: 1359 3962 1359
Size: 2603 3774
EarI CTCTTCn'nnn_
Cuts at: 754 2404 4400 4610 754
Size: 1650 1996 210 2521
Fragments arranged by size:
2521 1996 1650 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 769 1689 3073 4585 4895 5041 6187 769
Size: 920 1384 1512 310 146 1146 959
Fragments arranged by size:
1512 1384 1146 959 920 310 146
EcoICRI GAG'CTC
Cuts at: 1044 2877 3389 1044
Size: 1833 512 4032
Fragments arranged by size:
4032 1833 512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 288 534 1573 1876 2368 2530 2550 2692
Size: 18 246 1039 303 492 162 20 142
Cuts at: 2692 2851 2905 3007 3143 3221 3232 4202 4634
Size: 159 54 102 136 78 11 970 432
Cuts at: 4634 5381 5649 270
Size: 747 268 998
Fragments arranged by size:
1039 998 970 747 492 432 303 268
246 162 159 142 136 102 78 54
20 18 11
EcoNI CCTnn'n_nnAGG
Cuts at: 154 1399 1858 154
Size: 1245 459 4673
Fragments arranged by size:
4673 1245 459
EcoO109I rG'GnC_Cy
Cuts at: 168 169 297 407 744 1440 1805 2505 3134
Size: 1 128 110 337 696 365 700 629
Cuts at: 3134 3252 3516 168
Size: 118 264 3029
Fragments arranged by size:
3029 700 696 629 365 337 264 128
118 110 1
EcoRI G'AATT_C
Cuts at: 3381 3381
Size: 6377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4396 4396
Size: 6377
FspI TGC'GCA
Cuts at: 793 1288 1573 4158 793
Size: 495 285 2585 3012
Fragments arranged by size:
3012 2585 495 285
FspAI rTGC'GCAy
Cuts at: 1573 1573
Size: 6377
HaeII r_GCGC'y
Cuts at: 144 399 418 445 566 710 807 1701 3811
Size: 255 19 27 121 144 97 894 2110
Cuts at: 3811 4059 5081 6060 144
Size: 248 1022 979 461
Fragments arranged by size:
2110 1022 979 894 461 255 248 144
121 97 27 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1294 1326 1759 1791 4219 4249 4251 4281 1294
Size: 32 433 32 2428 30 2 30 3390
Fragments arranged by size:
3390 2428 433 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2817 3416 2817
Size: 599 5778
KasI G'GCGC_C
Cuts at: 706 3807 4055 706
Size: 3101 248 3028
Fragments arranged by size:
3101 3028 248
KpnI G_GTAC'C
Cuts at: 1587 2739 3397 1587
Size: 1152 658 4567
Fragments arranged by size:
4567 1152 658
MluI A'CGCG_T
Cuts at: 5810 5810
Size: 6377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1724 5048 5232 1724
Size: 3324 184 2869
Fragments arranged by size:
3324 2869 184
MscI TGG'CCA
Cuts at: 670 868 1000 1330 2173 3181 4138 670
Size: 198 132 330 843 1008 957 2909
Fragments arranged by size:
2909 1008 957 843 330 198 132
MslI CAynn'nnrTG
Cuts at: 850 856 953 1222 1234 1240 2935 3008 4366
Size: 6 97 269 12 6 1695 73 1358
Cuts at: 4366 4493 850
Size: 127 2734
Fragments arranged by size:
2734 1695 1358 269 127 97 73 12
6 6
MspA1I CmG'CkG
Cuts at: 225 663 996 1437 2300 2900 2944 3488 3802
Size: 438 333 441 863 600 44 544 314
Cuts at: 3802 4162 5175 5420 225
Size: 360 1013 245 1182
Fragments arranged by size:
1182 1013 863 600 544 441 438 360
333 314 245 44
NaeI GCC'GGC
Cuts at: 4558 4558
Size: 6377
NarI GG'CG_CC
Cuts at: 707 3808 4056 707
Size: 3101 248 3028
Fragments arranged by size:
3101 3028 248
NcoI C'CATG_G
Cuts at: 851 2645 3105 851
Size: 1794 460 4123
Fragments arranged by size:
4123 1794 460
NgoMIV G'CCGG_C
Cuts at: 4556 4556
Size: 6377
NspI r_CATG'y
Cuts at: 558 912 1089 1188 1239 1728 3001 3430 3930
Size: 354 177 99 51 489 1273 429 500
Cuts at: 3930 4837 558
Size: 907 2098
Fragments arranged by size:
2098 1273 907 500 489 429 354 177
99 51
PciI A'CATG_T
Cuts at: 1184 4833 1184
Size: 3649 2728
PflMI CCAn_nnn'nTGG
Cuts at: 603 2445 2985 603
Size: 1842 540 3995
Fragments arranged by size:
3995 1842 540
PfoI T'CCnGG_A
Cuts at: 1853 2497 1853
Size: 644 5733
PmeI GTTT'AAAC
Cuts at: 3376 3376
Size: 6377
PmlI CAC'GTG
Cuts at: 2694 2694
Size: 6377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5516 5548 5516
Size: 32 6345
PpuMI rG'GwC_Cy
Cuts at: 297 744 1440 2505 297
Size: 447 696 1065 4169
Fragments arranged by size:
4169 1065 696 447
PshAI GACnn'nnGTC
Cuts at: 1660 1660
Size: 6377
PspOMI G'GGCC_C
Cuts at: 168 1901 3134 168
Size: 1733 1233 3411
Fragments arranged by size:
3411 1733 1233
PspXI vC'TCGA_Gb
Cuts at: 1588 1588
Size: 6377
PstI C_TGCA'G
Cuts at: 662 2299 2949 3424 4109 662
Size: 1637 650 475 685 2930
Fragments arranged by size:
2930 1637 685 650 475
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6377
PvuII CAG'CTG
Cuts at: 663 996 2300 2944 3802 4162 663
Size: 333 1304 644 858 360 2878
Fragments arranged by size:
2878 1304 858 644 360 333
RsrII CG'GwC_CG
Cuts at: 4572 4572
Size: 6377
SacI G_AGCT'C
Cuts at: 1046 2879 3391 1046
Size: 1833 512 4032
Fragments arranged by size:
4032 1833 512
SalI G'TCGA_C
Cuts at: 3414 3414
Size: 6377
SanDI GG'GwC_CC
Cuts at: 297 1440 297
Size: 1143 5234
SapI GCTCTTCn'nnn_
Cuts at: 2404 4400 4610 2404
Size: 1996 210 4171
Fragments arranged by size:
4171 1996 210
SbfI CC_TGCA'GG
Cuts at: 3424 3424
Size: 6377
ScaI AGT'ACT
Cuts at: 5894 5894
Size: 6377
SexAI A'CCwGG_T
Cuts at: 1475 2390 2531 1475
Size: 915 141 5321
Fragments arranged by size:
5321 915 141
SfcI C'TryA_G
Cuts at: 33 658 2295 2945 3420 4105 5098 5289 33
Size: 625 1637 650 475 685 993 191 1121
Fragments arranged by size:
1637 1121 993 685 650 625 475 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2383 2383
Size: 6377
SfoI GGC'GCC
Cuts at: 708 3809 4057 708
Size: 3101 248 3028
Fragments arranged by size:
3101 3028 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6377
SmaI CCC'GGG
Cuts at: 742 1900 3399 742
Size: 1158 1499 3720
Fragments arranged by size:
3720 1499 1158
SmlI C'TyrA_G
Cuts at: 883 1450 1588 2058 3532 4447 4939 5201 883
Size: 567 138 470 1474 915 492 262 2059
Fragments arranged by size:
2059 1474 915 567 492 470 262 138
SnaBI TAC'GTA
Cuts at: 3369 3369
Size: 6377
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6377
SphI G_CATG'C
Cuts at: 558 912 1089 3001 3430 3930 558
Size: 354 177 1912 429 500 3005
Fragments arranged by size:
3005 1912 500 429 354 177
StuI AGG'CCT
Cuts at: 1680 2237 2444 1680
Size: 557 207 5613
Fragments arranged by size:
5613 557 207
StyI C'CwwG_G
Cuts at: 134 410 851 1016 1070 1535 1733 2439 2645
Size: 276 441 165 54 465 198 706 206
Cuts at: 2645 3105 3511 3865 134
Size: 460 406 354 2646
Fragments arranged by size:
2646 706 465 460 441 406 354 276
206 198 165 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 654 1905 1978 2316 3960 4093 4552 4761 654
Size: 1251 73 338 1644 133 459 209 2270
Fragments arranged by size:
2270 1644 1251 459 338 209 133 73
TatI w'GTAC_w
Cuts at: 533 2016 5892 533
Size: 1483 3876 1018
Fragments arranged by size:
3876 1483 1018
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 629 661 1306 1338 5894 5926 629
Size: 32 645 32 4556 32 1080
Fragments arranged by size:
4556 1080 645 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2755 4174 2755
Size: 1419 4958
XbaI T'CTAG_A
Cuts at: 3408 3408
Size: 6377
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 261 324 648 1023 2420 2529 3872 261
Size: 63 324 375 1397 109 1343 2766
Fragments arranged by size:
2766 1397 1343 375 324 109 63
XhoI C'TCGA_G
Cuts at: 1588 2058 1588
Size: 470 5907
XmaI C'CCGG_G
Cuts at: 740 1898 3397 740
Size: 1158 1499 3720
Fragments arranged by size:
3720 1499 1158
XmnI GAAnn'nnTTC
Cuts at: 2614 2614
Size: 6377
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BfrBI
BmtI BssHII BstZ17I ClaI CspCI EcoRV FseI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PsiI PsrI SacII SgrAI SrfI SspI SwaI
ZraI