Restriction map for
pF1KSDA0035
(Circular) (Six-base) MAPSORT of: pF1KSDA0035.seq Check: 1354 from: 1 to: 5266
pF1KSDA0035 5266 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1356 1356
Size: 5266
AccI GT'mk_AC
Cuts at: 2302 2302
Size: 5266
Acc65I G'GTAC_C
Cuts at: 1300 2280 1300
Size: 980 4286
AclI AA'CG_TT
Cuts at: 5106 5106
Size: 5266
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1175 1226 1954 3089 3521 4270 4538 1175
Size: 51 728 1135 432 749 268 1903
Fragments arranged by size:
1903 1135 749 728 432 268 51
AflIII A'CryG_T
Cuts at: 2616 3722 4699 2616
Size: 1106 977 3183
Fragments arranged by size:
3183 1106 977
AgeI A'CCGG_T
Cuts at: 3654 3654
Size: 5266
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1062 1094 1062
Size: 32 5234
AlwNI CAG_nnn'CTG
Cuts at: 880 1258 1678 2569 4138 880
Size: 378 420 891 1569 2008
Fragments arranged by size:
2008 1569 891 420 378
ApaLI G'TGCA_C
Cuts at: 4036 4036
Size: 5266
ApoI r'AATT_y
Cuts at: 1311 2268 4619 4875 1311
Size: 957 2351 256 1702
Fragments arranged by size:
2351 1702 957 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5266
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5266
AvaI C'yCGr_G
Cuts at: 734 2284 734
Size: 1550 3716
BamHI G'GATC_C
Cuts at: 2289 2289
Size: 5266
BanI G'GyrC_C
Cuts at: 1300 1798 2280 2694 2942 2977 1300
Size: 498 482 414 248 35 3589
Fragments arranged by size:
3589 498 482 414 248 35
BanII G_rGCy'C
Cuts at: 625 1240 1768 2278 3308 625
Size: 615 528 510 1030 2583
Fragments arranged by size:
2583 1030 615 528 510
BbeI G_GCGC'C
Cuts at: 2698 2946 2698
Size: 248 5018
BbsI GAAGACnn'nnnn_
Cuts at: 1130 1471 1612 1130
Size: 341 141 4784
Fragments arranged by size:
4784 341 141
BciVI GTATCCnnnnn_n'
Cuts at: 179 3162 3925 5266 179
Size: 2983 763 1341 179
Fragments arranged by size:
2983 1341 763 179
BclI T'GATC_A
Cuts at: 2784 2784
Size: 5266
BglI GCCn_nnn'nGGC
Cuts at: 1537 1789 1936 1537
Size: 252 147 4867
Fragments arranged by size:
4867 252 147
BglII A'GATC_T
Cuts at: 2779 4818 2779
Size: 2039 3227
BlpI GC'TnA_GC
Cuts at: 2376 2376
Size: 5266
Bme1580I G_kGCm'C
Cuts at: 2889 2982 4040 2889
Size: 93 1058 4115
Fragments arranged by size:
4115 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 907 2124 2476 2557 2892 5038 907
Size: 1217 352 81 335 2146 1135
Fragments arranged by size:
2146 1217 1135 352 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 981 1464 3631 981
Size: 483 2167 2616
Fragments arranged by size:
2616 2167 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 951 1199 1413 1434 1566 1713 410
Size: 541 248 214 21 132 147 3963
Fragments arranged by size:
3963 541 248 214 147 132 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 627 1738 2440 3319 3813 4111 283
Size: 344 1111 702 879 494 298 1438
Fragments arranged by size:
1438 1111 879 702 494 344 298
BsaI GGTCTCn'nnnn_
Cuts at: 4970 4970
Size: 5266
BsaAI yAC'GTr
Cuts at: 2256 2617 2256
Size: 361 4905
BsaBI GATnn'nnATC
Cuts at: 6 2778 4817 6
Size: 2772 2039 455
Fragments arranged by size:
2772 2039 455
BsaHI Gr'CG_yC
Cuts at: 2695 2943 3647 5143 2695
Size: 248 704 1496 2818
Fragments arranged by size:
2818 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2453 2974 3654 3928 4075 4486 2453
Size: 521 680 274 147 411 3233
Fragments arranged by size:
3233 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 631 661 1062 1092 1411 1441 631
Size: 30 401 30 319 30 4456
Fragments arranged by size:
4456 401 319 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 415 418 421 553 593 832 835 997 1471
Size: 3 3 132 40 239 3 162 474
Cuts at: 1471 1474 1532 1606 1609 1861 1864 1937 2059
Size: 3 58 74 3 252 3 73 122
Cuts at: 2059 415
Size: 3622
Fragments arranged by size:
3622 474 252 239 162 132 122 74
73 58 40 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 157 1073 2689 3447 4026 4522 157
Size: 916 1616 758 579 496 901
Fragments arranged by size:
1616 916 901 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1676 1790 2123 1676
Size: 114 333 4819
Fragments arranged by size:
4819 333 114
BsiEI CG_ry'CG
Cuts at: 104 134 2806 2852 4062 104
Size: 30 2672 46 1210 1308
Fragments arranged by size:
2672 1308 1210 46 30
BsiHKAI G_wGCw'C
Cuts at: 182 625 1240 2278 3056 3246 4040 182
Size: 443 615 1038 778 190 794 1408
Fragments arranged by size:
1408 1038 794 778 615 443 190
BsmI GAATG_Cn'
Cuts at: 140 140
Size: 5266
Bsp1286I G_dGCh'C
Cuts at: 182 625 1240 1768 2278 2889 2982 3056 3246
Size: 443 615 528 510 611 93 74 190
Cuts at: 3246 3308 4040 182
Size: 62 732 1408
Fragments arranged by size:
1408 732 615 611 528 510 443 190
93 74 62
BspEI T'CCGG_A
Cuts at: 4486 4486
Size: 5266
BspHI T'CATG_A
Cuts at: 4442 4442
Size: 5266
BspMI ACCTGCnnnn'nnnn_
Cuts at: 456 1356 2314 2528 2830 3211 456
Size: 900 958 214 302 381 2511
Fragments arranged by size:
2511 958 900 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3556 3610 5096 5261 50
Size: 3506 54 1486 165 55
Fragments arranged by size:
3506 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3176 3176
Size: 5266
BsrFI r'CCGG_y
Cuts at: 136 3262 3443 3654 136
Size: 3126 181 211 1748
Fragments arranged by size:
3126 1748 211 181
BssSI C'ACGA_G
Cuts at: 175 2066 3535 3895 175
Size: 1891 1469 360 1546
Fragments arranged by size:
1891 1546 1469 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1654 1813 2641 1654
Size: 159 828 4279
Fragments arranged by size:
4279 828 159
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5266
BstYI r'GATC_y
Cuts at: 1 2289 2756 2779 3114 3360 4374 4460 4472
Size: 2288 467 23 335 246 1014 86 12
Cuts at: 4472 4818 1
Size: 346 449
Fragments arranged by size:
2288 1014 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1544 1949 1544
Size: 405 4861
BtgI C'CryG_G
Cuts at: 125 125
Size: 5266
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3214 3395 3558 3214
Size: 181 163 4922
Fragments arranged by size:
4922 181 163
BtsI GCAGTG_nn'
Cuts at: 516 810 960 1125 1137 1358 1593 1883 2507
Size: 294 150 165 12 221 235 290 624
Cuts at: 2507 516
Size: 3275
Fragments arranged by size:
3275 624 294 290 235 221 165 150
12
DraI TTT'AAA
Cuts at: 2263 2263
Size: 5266
DrdI GACnn_nn'nnGTC
Cuts at: 1614 2970 3830 1614
Size: 1356 860 3050
Fragments arranged by size:
3050 1356 860
EaeI y'GGCC_r
Cuts at: 131 215 902 1392 1481 2849 3023 3414 3441
Size: 84 687 490 89 1368 174 391 27
Cuts at: 3441 4788 131
Size: 1347 609
Fragments arranged by size:
1368 1347 687 609 490 391 174 89
84 27
EagI C'GGCC_G
Cuts at: 131 2849 131
Size: 2718 2548
EarI CTCTTCn'nnn_
Cuts at: 273 509 937 974 1154 1245 1433 1595 1733
Size: 236 428 37 180 91 188 162 138
Cuts at: 1733 1739 3287 3497 273
Size: 6 1548 210 2042
Fragments arranged by size:
2042 1548 428 236 210 188 180 162
138 91 37 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3472 3784 3930 5076 3472
Size: 312 146 1146 3662
Fragments arranged by size:
3662 1146 312 146
EcoICRI GAG'CTC
Cuts at: 623 1238 2276 623
Size: 615 1038 3613
Fragments arranged by size:
3613 1038 615
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 951 1175 1199 1226 1413 1434 1566 1713
Size: 541 224 24 27 187 21 132 147
Cuts at: 1713 1954 3089 3521 4270 4538 410
Size: 241 1135 432 749 268 1138
Fragments arranged by size:
1138 1135 749 541 432 268 241 224
187 147 132 27 24 21
EcoO109I rG'GnC_Cy
Cuts at: 312 421 909 1015 2403 312
Size: 109 488 106 1388 3175
Fragments arranged by size:
3175 1388 488 109 106
EcoRI G'AATT_C
Cuts at: 1311 2268 1311
Size: 957 4309
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3283 3283
Size: 5266
FspI TGC'GCA
Cuts at: 3045 3045
Size: 5266
HaeII r_GCGC'y
Cuts at: 2698 2946 3970 4949 2698
Size: 248 1024 979 3015
Fragments arranged by size:
3015 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 625 631 657 663 1411 1443 1549 1581 1594
Size: 6 26 6 748 32 106 32 13
Cuts at: 1594 1626 2198 2230 3106 3136 3138 3168 625
Size: 32 572 32 876 30 2 30 2723
Fragments arranged by size:
2723 876 748 572 106 32 32 32
32 30 30 26 13 6 6 2
HincII GTy'rAC
Cuts at: 2303 2303
Size: 5266
HindIII A'AGCT_T
Cuts at: 1399 1966 1399
Size: 567 4699
KasI G'GCGC_C
Cuts at: 2694 2942 2694
Size: 248 5018
KpnI G_GTAC'C
Cuts at: 1304 2284 1304
Size: 980 4286
MluI A'CGCG_T
Cuts at: 4699 4699
Size: 5266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3937 4121 3937
Size: 184 5082
MscI TGG'CCA
Cuts at: 217 904 1394 1483 3025 217
Size: 687 490 89 1542 2458
Fragments arranged by size:
2458 1542 687 490 89
MslI CAynn'nnrTG
Cuts at: 3253 3380 3253
Size: 127 5139
MspA1I CmG'CkG
Cuts at: 1297 1330 1363 2375 2689 3049 4064 4309 1297
Size: 33 33 1012 314 360 1015 245 2254
Fragments arranged by size:
2254 1015 1012 360 314 245 33 33
NaeI GCC'GGC
Cuts at: 138 3445 138
Size: 3307 1959
NarI GG'CG_CC
Cuts at: 2695 2943 2695
Size: 248 5018
NcoI C'CATG_G
Cuts at: 125 125
Size: 5266
NgoMIV G'CCGG_C
Cuts at: 136 3443 136
Size: 3307 1959
NotI GC'GGCC_GC
Cuts at: 131 131
Size: 5266
NruI TCG'CGA
Cuts at: 3627 3627
Size: 5266
NspI r_CATG'y
Cuts at: 2317 2817 3726 2317
Size: 500 909 3857
Fragments arranged by size:
3857 909 500
PciI A'CATG_T
Cuts at: 3722 3722
Size: 5266
PmeI GTTT'AAAC
Cuts at: 2263 2263
Size: 5266
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1062 1094 4405 4437 1062
Size: 32 3311 32 1891
Fragments arranged by size:
3311 1891 32 32
PpuMI rG'GwC_Cy
Cuts at: 312 1015 312
Size: 703 4563
PspXI vC'TCGA_Gb
Cuts at: 734 734
Size: 5266
PstI C_TGCA'G
Cuts at: 486 1353 1368 1623 2311 2996 486
Size: 867 15 255 688 685 2756
Fragments arranged by size:
2756 867 688 685 255 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5266
PvuII CAG'CTG
Cuts at: 1297 1330 1363 2689 3049 1297
Size: 33 33 1326 360 3514
Fragments arranged by size:
3514 1326 360 33 33
RsrII CG'GwC_CG
Cuts at: 3459 3459
Size: 5266
SacI G_AGCT'C
Cuts at: 625 1240 2278 625
Size: 615 1038 3613
Fragments arranged by size:
3613 1038 615
SalI G'TCGA_C
Cuts at: 2301 2301
Size: 5266
SapI GCTCTTCn'nnn_
Cuts at: 1245 3287 3497 1245
Size: 2042 210 3014
Fragments arranged by size:
3014 2042 210
SbfI CC_TGCA'GG
Cuts at: 2311 2311
Size: 5266
ScaI AGT'ACT
Cuts at: 4783 4783
Size: 5266
SexAI A'CCwGG_T
Cuts at: 1011 1011
Size: 5266
SfcI C'TryA_G
Cuts at: 33 285 482 1020 1349 1364 1619 2307 2992
Size: 252 197 538 329 15 255 688 685
Cuts at: 2992 3987 4178 33
Size: 995 191 1121
Fragments arranged by size:
1121 995 688 685 538 329 255 252
197 191 15
SfoI GGC'GCC
Cuts at: 2696 2944 2696
Size: 248 5018
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5266
SmaI CCC'GGG
Cuts at: 2286 2286
Size: 5266
SmlI C'TyrA_G
Cuts at: 298 642 734 1753 2419 3334 3828 4090 298
Size: 344 92 1019 666 915 494 262 1474
Fragments arranged by size:
1474 1019 915 666 494 344 262 92
SnaBI TAC'GTA
Cuts at: 2256 2256
Size: 5266
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5266
SphI G_CATG'C
Cuts at: 2317 2817 2317
Size: 500 4766
StuI AGG'CCT
Cuts at: 370 370
Size: 5266
StyI C'CwwG_G
Cuts at: 125 308 587 905 1496 1787 1931 2398 2752
Size: 183 279 318 591 291 144 467 354
Cuts at: 2752 125
Size: 2639
Fragments arranged by size:
2639 591 467 354 318 291 279 183
144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 451 2847 2980 3439 3650 451
Size: 2396 133 459 211 2067
Fragments arranged by size:
2396 2067 459 211 133
TatI w'GTAC_w
Cuts at: 4781 4781
Size: 5266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4783 4815 4783
Size: 32 5234
Tth111I GACn'n_nGTC
Cuts at: 3061 3061
Size: 5266
XbaI T'CTAG_A
Cuts at: 2295 2295
Size: 5266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1089 1380 2759 1089
Size: 291 1379 3596
Fragments arranged by size:
3596 1379 291
XhoI C'TCGA_G
Cuts at: 734 734
Size: 5266
XmaI C'CCGG_G
Cuts at: 2284 2284
Size: 5266
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NruI NspI PciI PmeI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PspOMI PsrI SacII SanDI SfiI SgrAI
SrfI SspI SwaI XmnI ZraI