Restriction map for
pF1KSDA0033
(Circular) (Six-base) MAPSORT of: pF1KSDA0033.seq Check: 483 from: 1 to: 4010
pF1KSDA0033 4010 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 534 650 1048 534
Size: 116 398 3496
Fragments arranged by size:
3496 398 116
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1835 2267 3014 3282 1835
Size: 432 747 268 2563
Fragments arranged by size:
2563 747 432 268
AflIII A'CryG_T
Cuts at: 1362 2466 3443 1362
Size: 1104 977 1929
Fragments arranged by size:
1929 1104 977
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AhdI GACnn_n'nnGTC
Cuts at: 209 209
Size: 4010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 521 521
Size: 4010
AlwNI CAG_nnn'CTG
Cuts at: 1315 2882 1315
Size: 1567 2443
ApaLI G'TGCA_C
Cuts at: 2780 2780
Size: 4010
ApoI r'AATT_y
Cuts at: 345 991 1014 3363 3619 345
Size: 646 23 2349 256 736
Fragments arranged by size:
2349 736 646 256 23
AseI AT'TA_AT
Cuts at: 20 770 20
Size: 750 3260
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 1030 1030
Size: 4010
BamHI G'GATC_C
Cuts at: 1035 1035
Size: 4010
BanI G'GyrC_C
Cuts at: 219 601 1026 1440 1688 1723 219
Size: 382 425 414 248 35 2506
Fragments arranged by size:
2506 425 414 382 248 35
BanII G_rGCy'C
Cuts at: 1024 2054 1024
Size: 1030 2980
BbeI G_GCGC'C
Cuts at: 223 1444 1692 223
Size: 1221 248 2541
Fragments arranged by size:
2541 1221 248
BbvCI CC'TCA_GC
Cuts at: 353 353
Size: 4010
BciVI GTATCCnnnnn_n'
Cuts at: 1908 2669 4010 1908
Size: 761 1341 1908
Fragments arranged by size:
1908 1341 761
BclI T'GATC_A
Cuts at: 1530 1530
Size: 4010
BglI GCCn_nnn'nGGC
Cuts at: 862 862
Size: 4010
BglII A'GATC_T
Cuts at: 1525 3562 1525
Size: 2037 1973
BlpI GC'TnA_GC
Cuts at: 1122 1122
Size: 4010
Bme1580I G_kGCm'C
Cuts at: 1635 1728 2784 1635
Size: 93 1056 2861
Fragments arranged by size:
2861 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1222 1303 1638 3782 1222
Size: 81 335 2144 1450
Fragments arranged by size:
2144 1450 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 730 2377 730
Size: 1647 2363
Bpu10I CC'TnA_GC
Cuts at: 353 353
Size: 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2065 2557 2855 1186
Size: 879 492 298 2341
Fragments arranged by size:
2341 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 217 227 633 3714 217
Size: 10 406 3081 513
Fragments arranged by size:
3081 513 406 10
BsaAI yAC'GTr
Cuts at: 1002 1363 1002
Size: 361 3649
BsaBI GATnn'nnATC
Cuts at: 6 1524 3561 6
Size: 1518 2037 455
Fragments arranged by size:
2037 1518 455
BsaHI Gr'CG_yC
Cuts at: 220 1441 1689 2391 3887 220
Size: 1221 248 702 1496 343
Fragments arranged by size:
1496 1221 702 343 248
BsaWI w'CCGG_w
Cuts at: 1199 1720 2398 2672 2819 3230 1199
Size: 521 678 274 147 411 1979
Fragments arranged by size:
1979 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 890 920 890
Size: 30 3980
BseYI C'CCAG_C
Cuts at: 1435 2193 2770 3266 1435
Size: 758 577 496 2179
Fragments arranged by size:
2179 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 154 1552 1598 2806 104
Size: 50 1398 46 1208 1308
Fragments arranged by size:
1398 1308 1208 50 46
BsiHKAI G_wGCw'C
Cuts at: 1024 1802 1992 2784 1024
Size: 778 190 792 2250
Fragments arranged by size:
2250 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1024 1635 1728 1802 1992 2054 2784 1024
Size: 611 93 74 190 62 730 2250
Fragments arranged by size:
2250 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 681 1060 1274 1576 1957 681
Size: 379 214 302 381 2734
Fragments arranged by size:
2734 381 379 302 214
BsrBI CCG'CTC
Cuts at: 50 2302 2356 3840 4005 50
Size: 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1922 1922
Size: 4010
BsrFI r'CCGG_y
Cuts at: 2008 2189 2398 2008
Size: 181 209 3620
Fragments arranged by size:
3620 209 181
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 1387 1387
Size: 4010
BstBI TT'CG_AA
Cuts at: 110 2371 110
Size: 2261 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 399 399
Size: 4010
BstYI r'GATC_y
Cuts at: 1 1035 1502 1525 1860 2106 3118 3204 3216
Size: 1034 467 23 335 246 1012 86 12
Cuts at: 3216 3562 1
Size: 346 449
Fragments arranged by size:
1034 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 535 651 535
Size: 116 3894
BtgI C'CryG_G
Cuts at: 125 182 125
Size: 57 3953
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1960 2141 2304 1960
Size: 181 163 3666
Fragments arranged by size:
3666 181 163
BtsI GCAGTG_nn'
Cuts at: 1253 1253
Size: 4010
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 1595 1769 2160 2187 3532 1595
Size: 174 391 27 1345 2073
Fragments arranged by size:
2073 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2218 2528 2674 3820 2218
Size: 310 146 1146 2408
Fragments arranged by size:
2408 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1022 1022
Size: 4010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1835 2267 3014 3282 1835
Size: 432 747 268 2563
Fragments arranged by size:
2563 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1149 1149
Size: 4010
EcoRI G'AATT_C
Cuts at: 1014 1014
Size: 4010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 465 2029 465
Size: 1564 2446
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 167 223 1444 1692 2714 3693 167
Size: 56 1221 248 1022 979 484
Fragments arranged by size:
1221 1022 979 484 248 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 195 227 1852 1882 1884 1914 195
Size: 32 1625 30 2 30 2291
Fragments arranged by size:
2291 1625 32 30 30 2
HincII GTy'rAC
Cuts at: 1049 1049
Size: 4010
KasI G'GCGC_C
Cuts at: 219 1440 1688 219
Size: 1221 248 2541
Fragments arranged by size:
2541 1221 248
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2681 2865 2681
Size: 184 3826
MscI TGG'CCA
Cuts at: 1771 1771
Size: 4010
MslI CAynn'nnrTG
Cuts at: 1999 2126 1999
Size: 127 3883
MspA1I CmG'CkG
Cuts at: 199 1121 1435 1795 2808 3053 199
Size: 922 314 360 1013 245 1156
Fragments arranged by size:
1156 1013 922 360 314 245
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 220 1441 1689 220
Size: 1221 248 2541
Fragments arranged by size:
2541 1221 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4010
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NspI r_CATG'y
Cuts at: 1063 1563 2470 1063
Size: 500 907 2603
Fragments arranged by size:
2603 907 500
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PfoI T'CCnGG_A
Cuts at: 910 910
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PstI C_TGCA'G
Cuts at: 318 1057 1742 318
Size: 739 685 2586
Fragments arranged by size:
2586 739 685
PvuI CG_AT'CG
Cuts at: 104 154 104
Size: 50 3960
PvuII CAG'CTG
Cuts at: 1435 1795 1435
Size: 360 3650
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 1024 1024
Size: 4010
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 2033 2243 2033
Size: 210 3800
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 464 3527 464
Size: 3063 947
SfcI C'TryA_G
Cuts at: 33 314 1053 1738 2731 2922 33
Size: 281 739 685 993 191 1121
Fragments arranged by size:
1121 993 739 685 281 191
SfoI GGC'GCC
Cuts at: 221 1442 1690 221
Size: 1221 248 2541
Fragments arranged by size:
2541 1221 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 1032 1032
Size: 4010
SmlI C'TyrA_G
Cuts at: 1165 2080 2572 2834 1165
Size: 915 492 262 2341
Fragments arranged by size:
2341 915 492 262
SnaBI TAC'GTA
Cuts at: 1002 1002
Size: 4010
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
SspI AAT'ATT
Cuts at: 923 923
Size: 4010
StyI C'CwwG_G
Cuts at: 125 410 1144 1498 125
Size: 285 734 354 2637
Fragments arranged by size:
2637 734 354 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1593 1726 2185 2394 1593
Size: 133 459 209 3209
Fragments arranged by size:
3209 459 209 133
TatI w'GTAC_w
Cuts at: 462 3525 462
Size: 3063 947
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 3978
Tth111I GACn'n_nGTC
Cuts at: 1807 1807
Size: 4010
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1505 1505
Size: 4010
XmaI C'CCGG_G
Cuts at: 1030 1030
Size: 4010
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BpmI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
StuI SwaI XhoI XmnI ZraI