Restriction map for
pF1KSDA0028
(Circular) (Six-base) MAPSORT of: pF1KSDA0028.seq Check: 6373 from: 1 to: 5846
pF1KSDA0028 5846 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 771 771
Size: 5846
AccI GT'mk_AC
Cuts at: 277 437 2884 277
Size: 160 2447 3239
Fragments arranged by size:
3239 2447 160
Acc65I G'GTAC_C
Cuts at: 2862 2862
Size: 5846
AclI AA'CG_TT
Cuts at: 5686 5686
Size: 5846
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1055 1126 1331 1604 1701 3671 4103 4850 5118
Size: 71 205 273 97 1970 432 747 268
Cuts at: 5118 1055
Size: 1783
Fragments arranged by size:
1970 1783 747 432 273 268 205 97
71
AflIII A'CryG_T
Cuts at: 652 887 3198 4302 5279 652
Size: 235 2311 1104 977 1219
Fragments arranged by size:
2311 1219 1104 977 235
AgeI A'CCGG_T
Cuts at: 4234 4234
Size: 5846
AleI CACnn'nnGTG
Cuts at: 1537 1762 1537
Size: 225 5621
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2602 2634 2602
Size: 32 5814
AlwNI CAG_nnn'CTG
Cuts at: 355 2274 3151 4718 355
Size: 1919 877 1567 1483
Fragments arranged by size:
1919 1567 1483 877
ApaLI G'TGCA_C
Cuts at: 4616 4616
Size: 5846
ApoI r'AATT_y
Cuts at: 344 373 1250 2373 2681 2728 2850 5199 5455
Size: 29 877 1123 308 47 122 2349 256
Cuts at: 5455 344
Size: 735
Fragments arranged by size:
2349 1123 877 735 308 256 122 47
29
AseI AT'TA_AT
Cuts at: 20 20
Size: 5846
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5846
AvaI C'yCGr_G
Cuts at: 1415 2449 2705 2799 2866 1415
Size: 1034 256 94 67 4395
Fragments arranged by size:
4395 1034 256 94 67
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 817 850 1679 1712 817
Size: 33 829 33 4951
Fragments arranged by size:
4951 829 33 33
BamHI G'GATC_C
Cuts at: 2871 2871
Size: 5846
BanI G'GyrC_C
Cuts at: 1048 2541 2862 3276 3524 3559 1048
Size: 1493 321 414 248 35 3335
Fragments arranged by size:
3335 1493 414 321 248 35
BanII G_rGCy'C
Cuts at: 2860 3890 2860
Size: 1030 4816
BbeI G_GCGC'C
Cuts at: 3280 3528 3280
Size: 248 5598
BbvCI CC'TCA_GC
Cuts at: 2267 2294 2267
Size: 27 5819
BciVI GTATCCnnnnn_n'
Cuts at: 1680 3744 4505 5846 1680
Size: 2064 761 1341 1680
Fragments arranged by size:
2064 1680 1341 761
BclI T'GATC_A
Cuts at: 1863 2657 3366 1863
Size: 794 709 4343
Fragments arranged by size:
4343 794 709
BglI GCCn_nnn'nGGC
Cuts at: 1024 1024
Size: 5846
BglII A'GATC_T
Cuts at: 2519 3361 5398 2519
Size: 842 2037 2967
Fragments arranged by size:
2967 2037 842
BlpI GC'TnA_GC
Cuts at: 2958 2958
Size: 5846
Bme1580I G_kGCm'C
Cuts at: 1097 1495 1544 2475 2562 2693 3471 3564 4620
Size: 398 49 931 87 131 778 93 1056
Cuts at: 4620 1097
Size: 2323
Fragments arranged by size:
2323 1056 931 778 398 131 93 87
49
BmgBI CAC'GTC
Cuts at: 1440 2033 1440
Size: 593 5253
BmrI ACTGGGnnnn_n'
Cuts at: 745 753 1250 1497 1513 3058 3139 3474 5618
Size: 8 497 247 16 1545 81 335 2144
Cuts at: 5618 745
Size: 973
Fragments arranged by size:
2144 1545 973 497 335 247 81 16
8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2382 4213 2382
Size: 1831 4015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 1567 2575 833
Size: 734 1008 4104
Fragments arranged by size:
4104 1008 734
Bpu10I CC'TnA_GC
Cuts at: 2267 2294 2267
Size: 27 5819
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1137 3022 3901 4393 4691 1137
Size: 1885 879 492 298 2292
Fragments arranged by size:
2292 1885 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5550 5550
Size: 5846
BsaAI yAC'GTr
Cuts at: 381 653 1059 2838 3199 381
Size: 272 406 1779 361 3028
Fragments arranged by size:
3028 1779 406 361 272
BsaBI GATnn'nnATC
Cuts at: 6 3360 5397 6
Size: 3354 2037 455
Fragments arranged by size:
3354 2037 455
BsaHI Gr'CG_yC
Cuts at: 3277 3525 4227 5723 3277
Size: 248 702 1496 3400
Fragments arranged by size:
3400 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3035 3556 4234 4508 4655 5066 3035
Size: 521 678 274 147 411 3815
Fragments arranged by size:
3815 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 806 836 2308 2338 806
Size: 30 1472 30 4314
Fragments arranged by size:
4314 1472 30 30
BseYI C'CCAG_C
Cuts at: 632 709 1072 1181 2261 2748 3271 4029 4606
Size: 77 363 109 1080 487 523 758 577
Cuts at: 4606 5102 632
Size: 496 1376
Fragments arranged by size:
1376 1080 758 577 523 496 487 363
109 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 400
Size: 5846
BsiEI CG_ry'CG
Cuts at: 104 3388 3434 4642 104
Size: 3284 46 1208 1308
Fragments arranged by size:
3284 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2860 3638 3828 4620 2860
Size: 778 190 792 4086
Fragments arranged by size:
4086 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1430 1430
Size: 5846
Bsp1286I G_dGCh'C
Cuts at: 1097 1495 1544 2475 2562 2693 2860 3471 3564
Size: 398 49 931 87 131 167 611 93
Cuts at: 3564 3638 3828 3890 4620 1097
Size: 74 190 62 730 2323
Fragments arranged by size:
2323 931 730 611 398 190 167 131
93 87 74 62 49
BspEI T'CCGG_A
Cuts at: 5066 5066
Size: 5846
BspHI T'CATG_A
Cuts at: 5022 5022
Size: 5846
BspMI ACCTGCnnnn'nnnn_
Cuts at: 477 771 2896 3110 3412 3793 477
Size: 294 2125 214 302 381 2530
Fragments arranged by size:
2530 2125 381 302 294 214
BsrBI CCG'CTC
Cuts at: 50 4138 4192 5676 5841 50
Size: 4088 54 1484 165 55
Fragments arranged by size:
4088 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 280 2412 3758 280
Size: 2132 1346 2368
Fragments arranged by size:
2368 2132 1346
BsrFI r'CCGG_y
Cuts at: 3844 4025 4234 3844
Size: 181 209 5456
Fragments arranged by size:
5456 209 181
BsrGI T'GTAC_A
Cuts at: 1793 1793
Size: 5846
BssSI C'ACGA_G
Cuts at: 4117 4475 4117
Size: 358 5488
BstAPI GCAn_nnn'nTGC
Cuts at: 1145 2602 3223 1145
Size: 1457 621 3768
Fragments arranged by size:
3768 1457 621
BstBI TT'CG_AA
Cuts at: 110 4207 110
Size: 4097 1749
BstEII G'GTnAC_C
Cuts at: 2340 2340
Size: 5846
BstXI CCAn_nnnn'nTGG
Cuts at: 569 943 569
Size: 374 5472
BstYI r'GATC_y
Cuts at: 1 1977 2519 2871 3338 3361 3696 3942 4954
Size: 1976 542 352 467 23 335 246 1012
Cuts at: 4954 5040 5052 5398 1
Size: 86 12 346 449
Fragments arranged by size:
1976 1012 542 467 449 352 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 278 278
Size: 5846
Bsu36I CC'TnA_GG
Cuts at: 2633 2633
Size: 5846
BtgI C'CryG_G
Cuts at: 125 254 500 998 1613 125
Size: 129 246 498 615 4358
Fragments arranged by size:
4358 615 498 246 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1560 3796 3977 4140 1560
Size: 2236 181 163 3266
Fragments arranged by size:
3266 2236 181 163
BtsI GCAGTG_nn'
Cuts at: 1512 1769 3089 1512
Size: 257 1320 4269
Fragments arranged by size:
4269 1320 257
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2191 2226 2551 2586 2191
Size: 35 325 35 5451
Fragments arranged by size:
5451 325 35 35
DraI TTT'AAA
Cuts at: 2845 2845
Size: 5846
DrdI GACnn_nn'nnGTC
Cuts at: 3552 4410 3552
Size: 858 4988
EaeI y'GGCC_r
Cuts at: 1025 1061 1208 1610 1766 3431 3605 3996 4023
Size: 36 147 402 156 1665 174 391 27
Cuts at: 4023 5368 1025
Size: 1345 1503
Fragments arranged by size:
1665 1503 1345 402 391 174 156 147
36 27
EagI C'GGCC_G
Cuts at: 3431 3431
Size: 5846
EarI CTCTTCn'nnn_
Cuts at: 170 1417 3869 4079 170
Size: 1247 2452 210 1937
Fragments arranged by size:
2452 1937 1247 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1927 4054 4364 4510 5656 1927
Size: 2127 310 146 1146 2117
Fragments arranged by size:
2127 2117 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2858 2858
Size: 5846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 1055 1126 1331 1567 1604 1701 2575 3671
Size: 222 71 205 236 37 97 874 1096
Cuts at: 3671 4103 4850 5118 833
Size: 432 747 268 1561
Fragments arranged by size:
1561 1096 874 747 432 268 236 222
205 97 71 37
EcoNI CCTnn'n_nnAGG
Cuts at: 1644 1921 1644
Size: 277 5569
EcoO109I rG'GnC_Cy
Cuts at: 732 747 1191 1600 2985 732
Size: 15 444 409 1385 3593
Fragments arranged by size:
3593 1385 444 409 15
EcoRI G'AATT_C
Cuts at: 1250 2373 2850 1250
Size: 1123 477 4246
Fragments arranged by size:
4246 1123 477
EcoRV GAT'ATC
Cuts at: 2151 2151
Size: 5846
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1131 2169 2805 3865 1131
Size: 1038 636 1060 3112
Fragments arranged by size:
3112 1060 1038 636
FspI TGC'GCA
Cuts at: 3627 3627
Size: 5846
HaeII r_GCGC'y
Cuts at: 2539 3280 3528 4550 5529 2539
Size: 741 248 1022 979 2856
Fragments arranged by size:
2856 1022 979 741 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 669 701 2762 2794 3688 3718 3720 3750 669
Size: 32 2061 32 894 30 2 30 2765
Fragments arranged by size:
2765 2061 894 32 32 30 30 2
HincII GTy'rAC
Cuts at: 741 2885 741
Size: 2144 3702
HindIII A'AGCT_T
Cuts at: 2669 2669
Size: 5846
HpaI GTT'AAC
Cuts at: 741 741
Size: 5846
KasI G'GCGC_C
Cuts at: 3276 3524 3276
Size: 248 5598
KpnI G_GTAC'C
Cuts at: 2866 2866
Size: 5846
MluI A'CGCG_T
Cuts at: 887 5279 887
Size: 4392 1454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 550 4517 4701 550
Size: 3967 184 1695
Fragments arranged by size:
3967 1695 184
MscI TGG'CCA
Cuts at: 1063 1210 1612 3607 1063
Size: 147 402 1995 3302
Fragments arranged by size:
3302 1995 402 147
MslI CAynn'nnrTG
Cuts at: 431 1372 1537 1762 3835 3962 431
Size: 941 165 225 2073 127 2315
Fragments arranged by size:
2315 2073 941 225 165 127
MspA1I CmG'CkG
Cuts at: 632 1482 2271 2391 2957 3271 3631 4644 4889
Size: 850 789 120 566 314 360 1013 245
Cuts at: 4889 632
Size: 1589
Fragments arranged by size:
1589 1013 850 789 566 360 314 245
120
NaeI GCC'GGC
Cuts at: 4027 4027
Size: 5846
NarI GG'CG_CC
Cuts at: 3277 3525 3277
Size: 248 5598
NcoI C'CATG_G
Cuts at: 125 500 1613 125
Size: 375 1113 4358
Fragments arranged by size:
4358 1113 375
NgoMIV G'CCGG_C
Cuts at: 4025 4025
Size: 5846
NspI r_CATG'y
Cuts at: 930 1176 1816 2899 3399 4306 930
Size: 246 640 1083 500 907 2470
Fragments arranged by size:
2470 1083 907 640 500 246
PasI CC'CwG_GG
Cuts at: 1293 2182 1293
Size: 889 4957
PciI A'CATG_T
Cuts at: 4302 4302
Size: 5846
PflMI CCAn_nnn'nTGG
Cuts at: 506 1293 1525 506
Size: 787 232 4827
Fragments arranged by size:
4827 787 232
PfoI T'CCnGG_A
Cuts at: 225 225
Size: 5846
PmeI GTTT'AAAC
Cuts at: 2845 2845
Size: 5846
PmlI CAC'GTG
Cuts at: 1059 1059
Size: 5846
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4985 5017 4985
Size: 32 5814
PpuMI rG'GwC_Cy
Cuts at: 747 1191 747
Size: 444 5402
PstI C_TGCA'G
Cuts at: 2627 2893 3578 2627
Size: 266 685 4895
Fragments arranged by size:
4895 685 266
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5846
PvuII CAG'CTG
Cuts at: 632 2271 2391 3271 3631 632
Size: 1639 120 880 360 2847
Fragments arranged by size:
2847 1639 880 360 120
RsrII CG'GwC_CG
Cuts at: 4041 4041
Size: 5846
SacI G_AGCT'C
Cuts at: 2860 2860
Size: 5846
SalI G'TCGA_C
Cuts at: 2883 2883
Size: 5846
SanDI GG'GwC_CC
Cuts at: 747 747
Size: 5846
SapI GCTCTTCn'nnn_
Cuts at: 3869 4079 3869
Size: 210 5636
SbfI CC_TGCA'GG
Cuts at: 2893 2893
Size: 5846
ScaI AGT'ACT
Cuts at: 1260 5363 1260
Size: 4103 1743
SfcI C'TryA_G
Cuts at: 33 246 1960 2623 2889 3574 4567 4758 33
Size: 213 1714 663 266 685 993 191 1121
Fragments arranged by size:
1714 1121 993 685 663 266 213 191
SfoI GGC'GCC
Cuts at: 3278 3526 3278
Size: 248 5598
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5846
SmaI CCC'GGG
Cuts at: 1417 2707 2868 1417
Size: 1290 161 4395
Fragments arranged by size:
4395 1290 161
SmlI C'TyrA_G
Cuts at: 1116 3001 3916 4408 4670 1116
Size: 1885 915 492 262 2292
Fragments arranged by size:
2292 1885 915 492 262
SnaBI TAC'GTA
Cuts at: 2838 2838
Size: 5846
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5846
SphI G_CATG'C
Cuts at: 930 2899 3399 930
Size: 1969 500 3377
Fragments arranged by size:
3377 1969 500
SspI AAT'ATT
Cuts at: 585 585
Size: 5846
StuI AGG'CCT
Cuts at: 337 1916 2531 337
Size: 1579 615 3652
Fragments arranged by size:
3652 1579 615
StyI C'CwwG_G
Cuts at: 125 500 1613 1645 1911 2980 3334 125
Size: 375 1113 32 266 1069 354 2637
Fragments arranged by size:
2637 1113 1069 375 354 266 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1420 1617 2362 2740 3429 3562 4021 4230 1420
Size: 197 745 378 689 133 459 209 3036
Fragments arranged by size:
3036 745 689 459 378 209 197 133
TatI w'GTAC_w
Cuts at: 1258 1793 2315 5361 1258
Size: 535 522 3046 1743
Fragments arranged by size:
3046 1743 535 522
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 806 838 2140 2172 2452 2484 2541 2573 5363
Size: 32 1302 32 280 32 57 32 2790
Cuts at: 5363 5395 806
Size: 32 1257
Fragments arranged by size:
2790 1302 1257 280 57 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2719 3643 2719
Size: 924 4922
XbaI T'CTAG_A
Cuts at: 2877 2877
Size: 5846
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 780 1218 3341 780
Size: 438 2123 3285
Fragments arranged by size:
3285 2123 438
XmaI C'CCGG_G
Cuts at: 1415 2705 2866 1415
Size: 1290 161 4395
Fragments arranged by size:
4395 1290 161
XmnI GAAnn'nnTTC
Cuts at: 2732 2788 2732
Size: 56 5790
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BbsI BcgI BfrBI BmtI BseRI BsiWI BsmI BssHII
ClaI DraIII FseI FspAI MfeI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SrfI SwaI XhoI ZraI