Restriction map for
pF1KSDA0014
(Circular) (Six-base) MAPSORT of: pF1KSDA0014.seq Check: 4252 from: 1 to: 4616
pF1KSDA0014 4616 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 147 147
Size: 4616
AccI GT'mk_AC
Cuts at: 1654 1654
Size: 4616
Acc65I G'GTAC_C
Cuts at: 1313 1632 1313
Size: 319 4297
AclI AA'CG_TT
Cuts at: 4456 4456
Size: 4616
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1581 2441 2873 3620 3888 1581
Size: 860 432 747 268 2309
Fragments arranged by size:
2309 860 747 432 268
AflIII A'CryG_T
Cuts at: 281 852 1968 3072 4049 281
Size: 571 1116 1104 977 848
Fragments arranged by size:
1116 1104 977 848 571
AgeI A'CCGG_T
Cuts at: 3004 3004
Size: 4616
AhdI GACnn_n'nnGTC
Cuts at: 1247 1247
Size: 4616
AleI CACnn'nnGTG
Cuts at: 136 136
Size: 4616
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 718 718
Size: 4616
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 637 669 637
Size: 32 4584
AlwNI CAG_nnn'CTG
Cuts at: 162 192 315 427 598 774 873 1200 1921
Size: 30 123 112 171 176 99 327 721
Cuts at: 1921 3488 162
Size: 1567 1290
Fragments arranged by size:
1567 1290 721 327 176 171 123 112
99 30
ApaLI G'TGCA_C
Cuts at: 1405 3386 1405
Size: 1981 2635
ApoI r'AATT_y
Cuts at: 1620 3969 4225 1620
Size: 2349 256 2011
Fragments arranged by size:
2349 2011 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4616
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
AvaI C'yCGr_G
Cuts at: 1636 1636
Size: 4616
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 268 301 268
Size: 33 4583
BamHI G'GATC_C
Cuts at: 513 1641 513
Size: 1128 3488
BanI G'GyrC_C
Cuts at: 520 1185 1313 1632 2046 2294 2329 520
Size: 665 128 319 414 248 35 2807
Fragments arranged by size:
2807 665 414 319 248 128 35
BanII G_rGCy'C
Cuts at: 992 1003 1054 1123 1630 2660 992
Size: 11 51 69 507 1030 2948
Fragments arranged by size:
2948 1030 507 69 51 11
BbeI G_GCGC'C
Cuts at: 2050 2298 2050
Size: 248 4368
BbsI GAAGACnn'nnnn_
Cuts at: 259 259
Size: 4616
BbvCI CC'TCA_GC
Cuts at: 731 731
Size: 4616
BciVI GTATCCnnnnn_n'
Cuts at: 2514 3275 4616 2514
Size: 761 1341 2514
Fragments arranged by size:
2514 1341 761
BclI T'GATC_A
Cuts at: 842 2136 842
Size: 1294 3322
BfrBI ATG'CAT
Cuts at: 566 566
Size: 4616
BglI GCCn_nnn'nGGC
Cuts at: 187 830 1328 187
Size: 643 498 3475
Fragments arranged by size:
3475 643 498
BglII A'GATC_T
Cuts at: 2131 4168 2131
Size: 2037 2579
BlpI GC'TnA_GC
Cuts at: 991 1036 1388 1728 991
Size: 45 352 340 3879
Fragments arranged by size:
3879 352 340 45
Bme1580I G_kGCm'C
Cuts at: 333 348 1409 1548 2241 2334 3390 333
Size: 15 1061 139 693 93 1056 1559
Fragments arranged by size:
1559 1061 1056 693 139 93 15
BmrI ACTGGGnnnn_n'
Cuts at: 1828 1909 2244 4388 1828
Size: 81 335 2144 2056
Fragments arranged by size:
2144 2056 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2983 2983
Size: 4616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1087 1255 1489 1078
Size: 9 168 234 4205
Fragments arranged by size:
4205 234 168 9
Bpu10I CC'TnA_GC
Cuts at: 731 731
Size: 4616
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 1123 1339 1792 2671 3163 3461 165
Size: 958 216 453 879 492 298 1320
Fragments arranged by size:
1320 958 879 492 453 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 4320 4320
Size: 4616
BsaAI yAC'GTr
Cuts at: 284 855 1608 1969 284
Size: 571 753 361 2931
Fragments arranged by size:
2931 753 571 361
BsaBI GATnn'nnATC
Cuts at: 6 2130 4167 6
Size: 2124 2037 455
Fragments arranged by size:
2124 2037 455
BsaHI Gr'CG_yC
Cuts at: 2047 2295 2997 4493 2047
Size: 248 702 1496 2170
Fragments arranged by size:
2170 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1310 1805 2326 3004 3278 3425 3836 1310
Size: 495 521 678 274 147 411 2090
Fragments arranged by size:
2090 678 521 495 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 637 667 637
Size: 30 4586
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 993 1501 993
Size: 508 4108
BseYI C'CCAG_C
Cuts at: 572 1265 2041 2799 3376 3872 572
Size: 693 776 758 577 496 1316
Fragments arranged by size:
1316 776 758 693 577 496
BsiEI CG_ry'CG
Cuts at: 104 2158 2204 3412 104
Size: 2054 46 1208 1308
Fragments arranged by size:
2054 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 151 992 1086 1409 1556 1630 2408 2598 3390
Size: 841 94 323 147 74 778 190 792
Cuts at: 3390 151
Size: 1377
Fragments arranged by size:
1377 841 792 778 323 190 147 94
74
Bsp1286I G_dGCh'C
Cuts at: 151 333 348 992 1003 1054 1086 1123 1409
Size: 182 15 644 11 51 32 37 286
Cuts at: 1409 1548 1556 1630 2241 2334 2408 2598 2660
Size: 139 8 74 611 93 74 190 62
Cuts at: 2660 3390 151
Size: 730 1377
Fragments arranged by size:
1377 730 644 611 286 190 182 139
93 74 74 62 51 37 32 15
11 8
BspEI T'CCGG_A
Cuts at: 3836 3836
Size: 4616
BspHI T'CATG_A
Cuts at: 3792 3792
Size: 4616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 147 644 731 746 812 1666 1880 2182 2563
Size: 497 87 15 66 854 214 302 381
Cuts at: 2563 147
Size: 2200
Fragments arranged by size:
2200 854 497 381 302 214 87 66
15
BsrBI CCG'CTC
Cuts at: 50 360 2908 2962 4446 4611 50
Size: 310 2548 54 1484 165 55
Fragments arranged by size:
2548 1484 310 165 55 54
BsrDI GCAATG_nn'
Cuts at: 566 2528 566
Size: 1962 2654
BsrFI r'CCGG_y
Cuts at: 2614 2795 3004 2614
Size: 181 209 4226
Fragments arranged by size:
4226 209 181
BssSI C'ACGA_G
Cuts at: 2887 3245 2887
Size: 358 4258
BstAPI GCAn_nnn'nTGC
Cuts at: 162 465 1524 1993 162
Size: 303 1059 469 2785
Fragments arranged by size:
2785 1059 469 303
BstBI TT'CG_AA
Cuts at: 110 2977 110
Size: 2867 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 618 618
Size: 4616
BstYI r'GATC_y
Cuts at: 1 513 1641 2108 2131 2466 2712 3724 3810
Size: 512 1128 467 23 335 246 1012 86
Cuts at: 3810 3822 4168 1
Size: 12 346 449
Fragments arranged by size:
1128 1012 512 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1094 1536 1094
Size: 442 4174
BtgI C'CryG_G
Cuts at: 617 923 1343 1562 617
Size: 306 420 219 3671
Fragments arranged by size:
3671 420 306 219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2566 2747 2910 2566
Size: 181 163 4272
Fragments arranged by size:
4272 181 163
BtsI GCAGTG_nn'
Cuts at: 172 333 1859 172
Size: 161 1526 2929
Fragments arranged by size:
2929 1526 161
DraI TTT'AAA
Cuts at: 1615 1615
Size: 4616
DraIII CAC_nnn'GTG
Cuts at: 897 897
Size: 4616
DrdI GACnn_nn'nnGTC
Cuts at: 2322 3180 2322
Size: 858 3758
EaeI y'GGCC_r
Cuts at: 875 1274 1444 2201 2375 2766 2793 4138 875
Size: 399 170 757 174 391 27 1345 1353
Fragments arranged by size:
1353 1345 757 399 391 174 170 27
EagI C'GGCC_G
Cuts at: 2201 2201
Size: 4616
EarI CTCTTCn'nnn_
Cuts at: 220 2639 2849 220
Size: 2419 210 1987
Fragments arranged by size:
2419 1987 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2824 3134 3280 4426 2824
Size: 310 146 1146 3014
Fragments arranged by size:
3014 1146 310 146
EcoICRI GAG'CTC
Cuts at: 990 1628 990
Size: 638 3978
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1078 1087 1255 1489 1581 2441 2873 3620 3888
Size: 9 168 234 92 860 432 747 268
Cuts at: 3888 1078
Size: 1806
Fragments arranged by size:
1806 860 747 432 268 234 168 92
9
EcoNI CCTnn'n_nnAGG
Cuts at: 352 670 1468 352
Size: 318 798 3500
Fragments arranged by size:
3500 798 318
EcoO109I rG'GnC_Cy
Cuts at: 189 720 735 1098 1755 189
Size: 531 15 363 657 3050
Fragments arranged by size:
3050 657 531 363 15
EcoRI G'AATT_C
Cuts at: 1620 1620
Size: 4616
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 255 2635 255
Size: 2380 2236
FspI TGC'GCA
Cuts at: 748 2397 748
Size: 1649 2967
HaeII r_GCGC'y
Cuts at: 2050 2298 3320 4299 2050
Size: 248 1022 979 2367
Fragments arranged by size:
2367 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1233 1265 2458 2488 2490 2520 1233
Size: 32 1193 30 2 30 3329
Fragments arranged by size:
3329 1193 32 30 30 2
HincII GTy'rAC
Cuts at: 1655 1655
Size: 4616
KasI G'GCGC_C
Cuts at: 2046 2294 2046
Size: 248 4368
KpnI G_GTAC'C
Cuts at: 1317 1636 1317
Size: 319 4297
MluI A'CGCG_T
Cuts at: 4049 4049
Size: 4616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1127 3287 3471 1127
Size: 2160 184 2272
Fragments arranged by size:
2272 2160 184
MscI TGG'CCA
Cuts at: 877 1276 2377 877
Size: 399 1101 3116
Fragments arranged by size:
3116 1101 399
MslI CAynn'nnrTG
Cuts at: 136 529 616 2605 2732 136
Size: 393 87 1989 127 2020
Fragments arranged by size:
2020 1989 393 127 87
MspA1I CmG'CkG
Cuts at: 178 297 315 702 1032 1182 1269 1524 1727
Size: 119 18 387 330 150 87 255 203
Cuts at: 1727 2041 2401 3414 3659 178
Size: 314 360 1013 245 1135
Fragments arranged by size:
1135 1013 387 360 330 314 255 245
203 150 119 87 18
NaeI GCC'GGC
Cuts at: 2797 2797
Size: 4616
NarI GG'CG_CC
Cuts at: 2047 2295 2047
Size: 248 4368
NcoI C'CATG_G
Cuts at: 1343 1343
Size: 4616
NgoMIV G'CCGG_C
Cuts at: 2795 2795
Size: 4616
NsiI A_TGCA'T
Cuts at: 568 568
Size: 4616
NspI r_CATG'y
Cuts at: 381 463 534 566 1129 1669 2169 3076 381
Size: 82 71 32 563 540 500 907 1921
Fragments arranged by size:
1921 907 563 540 500 82 71 32
PciI A'CATG_T
Cuts at: 3072 3072
Size: 4616
PflMI CCAn_nnn'nTGG
Cuts at: 873 1272 873
Size: 399 4217
PmeI GTTT'AAAC
Cuts at: 1615 1615
Size: 4616
PmlI CAC'GTG
Cuts at: 284 855 284
Size: 571 4045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 637 669 3755 3787 637
Size: 32 3086 32 1466
Fragments arranged by size:
3086 1466 32 32
PpuMI rG'GwC_Cy
Cuts at: 720 735 1098 720
Size: 15 363 4238
Fragments arranged by size:
4238 363 15
PstI C_TGCA'G
Cuts at: 167 340 356 454 770 1049 1663 2348 167
Size: 173 16 98 316 279 614 685 2435
Fragments arranged by size:
2435 685 614 316 279 173 98 16
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4616
PvuII CAG'CTG
Cuts at: 178 315 1032 1182 1269 1524 2041 2401 178
Size: 137 717 150 87 255 517 360 2393
Fragments arranged by size:
2393 717 517 360 255 150 137 87
RsrII CG'GwC_CG
Cuts at: 2811 2811
Size: 4616
SacI G_AGCT'C
Cuts at: 992 1630 992
Size: 638 3978
SalI G'TCGA_C
Cuts at: 1653 1653
Size: 4616
SapI GCTCTTCn'nnn_
Cuts at: 2639 2849 2639
Size: 210 4406
SbfI CC_TGCA'GG
Cuts at: 356 1663 356
Size: 1307 3309
ScaI AGT'ACT
Cuts at: 4133 4133
Size: 4616
SfcI C'TryA_G
Cuts at: 33 163 319 336 352 450 766 1045 1659
Size: 130 156 17 16 98 316 279 614
Cuts at: 1659 2344 3337 3528 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 614 316 279 191 156
130 98 17 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 830 830
Size: 4616
SfoI GGC'GCC
Cuts at: 2048 2296 2048
Size: 248 4368
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4616
SmaI CCC'GGG
Cuts at: 1638 1638
Size: 4616
SmlI C'TyrA_G
Cuts at: 144 1138 1354 1771 2686 3178 3440 144
Size: 994 216 417 915 492 262 1320
Fragments arranged by size:
1320 994 915 492 417 262 216
SnaBI TAC'GTA
Cuts at: 1608 1608
Size: 4616
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4616
SphI G_CATG'C
Cuts at: 381 463 1669 2169 381
Size: 82 1206 500 2828
Fragments arranged by size:
2828 1206 500 82
StuI AGG'CCT
Cuts at: 1474 1474
Size: 4616
StyI C'CwwG_G
Cuts at: 1317 1343 1750 2104 1317
Size: 26 407 354 3829
Fragments arranged by size:
3829 407 354 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1279 2199 2332 2791 3000 1279
Size: 920 133 459 209 2895
Fragments arranged by size:
2895 920 459 209 133
TatI w'GTAC_w
Cuts at: 541 4131 541
Size: 3590 1026
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1271 1303 4133 4165 1271
Size: 32 2830 32 1722
Fragments arranged by size:
2830 1722 32 32
Tth111I GACn'n_nGTC
Cuts at: 2413 2413
Size: 4616
XbaI T'CTAG_A
Cuts at: 1647 1647
Size: 4616
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 580 898 2111 580
Size: 318 1213 3085
Fragments arranged by size:
3085 1213 318
XmaI C'CCGG_G
Cuts at: 1636 1636
Size: 4616
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII ApaI AscI AvrII BcgI BmgBI
BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PasI PfoI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SgrAI SrfI SspI SwaI XhoI XmnI ZraI