(Circular) (Six-base) MAPSORT of: pF1KOF.seq Check: 3048 from: 1 to: 3450 With 182 enzymes: SgfI * September 4, 2008 14:04 .. AatII G_ACGT'C Cuts at: 245 245 Size: 3450 AccI GT'mk_AC Cuts at: 488 488 Size: 3450 Acc65I G'GTAC_C Cuts at: 466 466 Size: 3450 AclI AA'CG_TT Cuts at: 3290 3290 Size: 3450 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 136 339 1275 1707 2060 2722 136 Size: 203 936 432 353 662 864 Fragments arranged by size: 936 864 662 432 353 203 AflIII A'CryG_T Cuts at: 125 802 2602 2883 125 Size: 677 1800 281 692 Fragments arranged by size: 1800 692 677 281 AlwNI CAG_nnn'CTG Cuts at: 755 2193 755 Size: 1438 2012 ApaLI G'TGCA_C Cuts at: 2288 2288 Size: 3450 ApoI r'AATT_y Cuts at: 360 454 2803 3059 360 Size: 94 2349 256 751 Fragments arranged by size: 2349 751 256 94 AseI AT'TA_AT Cuts at: 20 20 Size: 3450 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3450 AvaI C'yCGr_G Cuts at: 470 470 Size: 3450 BamHI G'GATC_C Cuts at: 475 475 Size: 3450 BanI G'GyrC_C Cuts at: 466 880 1128 1163 466 Size: 414 248 35 2753 Fragments arranged by size: 2753 414 248 35 BanII G_rGCy'C Cuts at: 464 1494 464 Size: 1030 2420 BbeI G_GCGC'C Cuts at: 884 1132 884 Size: 248 3202 BciVI GTATCCnnnnn_n' Cuts at: 1348 2404 3450 1348 Size: 1056 1046 1348 Fragments arranged by size: 1348 1056 1046 BclI T'GATC_A Cuts at: 970 970 Size: 3450 BglII A'GATC_T Cuts at: 965 3002 965 Size: 2037 1413 BlpI GC'TnA_GC Cuts at: 562 562 Size: 3450 Bme1580I G_kGCm'C Cuts at: 1075 1168 2292 1075 Size: 93 1124 2233 Fragments arranged by size: 2233 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 662 743 1078 3222 662 Size: 81 335 2144 890 Fragments arranged by size: 2144 890 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1817 1817 Size: 3450 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 283 283 Size: 3450 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 366 626 1505 2219 2517 366 Size: 260 879 714 298 1299 Fragments arranged by size: 1299 879 714 298 260 BsaI GGTCTCn'nnnn_ Cuts at: 3154 3154 Size: 3450 BsaAI yAC'GTr Cuts at: 803 803 Size: 3450 BsaBI GATnn'nnATC Cuts at: 6 964 3001 6 Size: 958 2037 455 Fragments arranged by size: 2037 958 455 BsaHI Gr'CG_yC Cuts at: 242 881 1129 1831 3327 242 Size: 639 248 702 1496 365 Fragments arranged by size: 1496 702 639 365 248 BsaWI w'CCGG_w Cuts at: 368 639 1160 1838 2249 2396 2670 368 Size: 271 521 678 411 147 274 1148 Fragments arranged by size: 1148 678 521 411 274 271 147 BseYI C'CCAG_C Cuts at: 212 875 1633 2298 2706 212 Size: 663 758 665 408 956 Fragments arranged by size: 956 758 665 663 408 BsiEI CG_ry'CG Cuts at: 104 992 1038 2268 104 Size: 888 46 1230 1286 Fragments arranged by size: 1286 1230 888 46 BsiHKAI G_wGCw'C Cuts at: 464 1242 1432 2292 464 Size: 778 190 860 1622 Fragments arranged by size: 1622 860 778 190 Bsp1286I G_dGCh'C Cuts at: 464 1075 1168 1242 1432 1494 2292 464 Size: 611 93 74 190 62 798 1622 Fragments arranged by size: 1622 798 611 190 93 74 62 BspHI T'CATG_A Cuts at: 1882 1882 Size: 3450 BspMI ACCTGCnnnn'nnnn_ Cuts at: 500 714 1016 1397 500 Size: 214 302 381 2553 Fragments arranged by size: 2553 381 302 214 BsrBI CCG'CTC Cuts at: 50 1742 1796 3280 3445 50 Size: 1692 54 1484 165 55 Fragments arranged by size: 1692 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1362 1362 Size: 3450 BsrFI r'CCGG_y Cuts at: 1448 1629 1448 Size: 181 3269 BssSI C'ACGA_G Cuts at: 1721 2429 1721 Size: 708 2742 BstAPI GCAn_nnn'nTGC Cuts at: 827 827 Size: 3450 BstBI TT'CG_AA Cuts at: 1811 1811 Size: 3450 BstYI r'GATC_y Cuts at: 1 475 942 965 1300 1546 1852 1864 1950 Size: 474 467 23 335 246 306 12 86 Cuts at: 1950 3002 1 Size: 1052 449 Fragments arranged by size: 1052 474 467 449 335 306 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 3450 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1400 1581 1744 1400 Size: 181 163 3106 Fragments arranged by size: 3106 181 163 BtsI GCAGTG_nn' Cuts at: 693 693 Size: 3450 DraI TTT'AAA Cuts at: 449 449 Size: 3450 DrdI GACnn_nn'nnGTC Cuts at: 1156 2500 1156 Size: 1344 2106 EaeI y'GGCC_r Cuts at: 1035 1209 1600 1627 2972 1035 Size: 174 391 27 1345 1513 Fragments arranged by size: 1513 1345 391 174 27 EagI C'GGCC_G Cuts at: 1035 1035 Size: 3450 EarI CTCTTCn'nnn_ Cuts at: 1473 1683 1473 Size: 210 3240 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 281 1658 2400 2546 3260 281 Size: 1377 742 146 714 471 Fragments arranged by size: 1377 742 714 471 146 EcoICRI GAG'CTC Cuts at: 462 462 Size: 3450 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 136 283 339 1275 1707 2060 2722 136 Size: 147 56 936 432 353 662 864 Fragments arranged by size: 936 864 662 432 353 147 56 EcoO109I rG'GnC_Cy Cuts at: 589 589 Size: 3450 EcoRI G'AATT_C Cuts at: 454 454 Size: 3450 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 250 1469 250 Size: 1219 2231 FspI TGC'GCA Cuts at: 1231 1231 Size: 3450 HaeII r_GCGC'y Cuts at: 884 1132 2362 3133 884 Size: 248 1230 771 1201 Fragments arranged by size: 1230 1201 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 262 294 1292 1322 1324 1354 262 Size: 32 998 30 2 30 2358 Fragments arranged by size: 2358 998 32 30 30 2 HincII GTy'rAC Cuts at: 489 489 Size: 3450 KasI G'GCGC_C Cuts at: 880 1128 880 Size: 248 3202 KpnI G_GTAC'C Cuts at: 470 470 Size: 3450 MluI A'CGCG_T Cuts at: 2883 2883 Size: 3450 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2209 2393 2209 Size: 184 3266 MscI TGG'CCA Cuts at: 1211 1211 Size: 3450 MslI CAynn'nnrTG Cuts at: 1439 1566 1439 Size: 127 3323 MspA1I CmG'CkG Cuts at: 561 875 1235 2019 2264 561 Size: 314 360 784 245 1747 Fragments arranged by size: 1747 784 360 314 245 NaeI GCC'GGC Cuts at: 1631 1631 Size: 3450 NarI GG'CG_CC Cuts at: 881 1129 881 Size: 248 3202 NcoI C'CATG_G Cuts at: 107 107 Size: 3450 NgoMIV G'CCGG_C Cuts at: 1629 1629 Size: 3450 NspI r_CATG'y Cuts at: 503 1003 2606 503 Size: 500 1603 1347 Fragments arranged by size: 1603 1347 500 PciI A'CATG_T Cuts at: 2602 2602 Size: 3450 PmeI GTTT'AAAC Cuts at: 449 449 Size: 3450 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1896 1928 1896 Size: 32 3418 PstI C_TGCA'G Cuts at: 497 1182 497 Size: 685 2765 PvuI CG_AT'CG Cuts at: 104 104 Size: 3450 PvuII CAG'CTG Cuts at: 875 1235 875 Size: 360 3090 RsrII CG'GwC_CG Cuts at: 1645 1645 Size: 3450 SacI G_AGCT'C Cuts at: 464 464 Size: 3450 SalI G'TCGA_C Cuts at: 487 487 Size: 3450 SapI GCTCTTCn'nnn_ Cuts at: 1473 1683 1473 Size: 210 3240 SbfI CC_TGCA'GG Cuts at: 497 497 Size: 3450 ScaI AGT'ACT Cuts at: 2967 2967 Size: 3450 SfcI C'TryA_G Cuts at: 33 493 1178 2146 2337 33 Size: 460 685 968 191 1146 Fragments arranged by size: 1146 968 685 460 191 SfoI GGC'GCC Cuts at: 882 1130 882 Size: 248 3202 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3450 SmaI CCC'GGG Cuts at: 472 472 Size: 3450 SmlI C'TyrA_G Cuts at: 381 605 1520 2234 2496 381 Size: 224 915 714 262 1335 Fragments arranged by size: 1335 915 714 262 224 SpeI A'CTAG_T Cuts at: 67 67 Size: 3450 SphI G_CATG'C Cuts at: 503 1003 503 Size: 500 2950 StyI C'CwwG_G Cuts at: 107 584 938 107 Size: 477 354 2619 Fragments arranged by size: 2619 477 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 204 1033 1166 1625 1834 204 Size: 829 133 459 209 1820 Fragments arranged by size: 1820 829 459 209 133 TatI w'GTAC_w Cuts at: 2965 2965 Size: 3450 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2967 2999 2967 Size: 32 3418 Tth111I GACn'n_nGTC Cuts at: 1247 1247 Size: 3450 XbaI T'CTAG_A Cuts at: 481 481 Size: 3450 XcmI CCAnnnn_n'nnnnTGG Cuts at: 945 945 Size: 3450 XmaI C'CCGG_G Cuts at: 470 470 Size: 3450 ZraI GAC'GTC Cuts at: 243 243 Size: 3450 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AarI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI