Restriction map for
pF1KF0188
(Circular) (Six-base) MAPSORT of: pF1KF0188.seq Check: 5525 from: 1 to: 6944
pF1KF0188 6944 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3285 3285
Size: 6944
AccI GT'mk_AC
Cuts at: 3169 3982 3169
Size: 813 6131
Acc65I G'GTAC_C
Cuts at: 960 2369 3960 960
Size: 1409 1591 3944
Fragments arranged by size:
3944 1591 1409
AclI AA'CG_TT
Cuts at: 2270 6784 2270
Size: 4514 2430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 910 1144 1940 2021 2261 2319 4769 5201
Size: 432 234 796 81 240 58 2450 432
Cuts at: 5201 5554 6216 478
Size: 353 662 1206
Fragments arranged by size:
2450 1206 796 662 432 432 353 240
234 81 58
AflII C'TTAA_G
Cuts at: 2922 2922
Size: 6944
AflIII A'CryG_T
Cuts at: 4296 6096 6377 4296
Size: 1800 281 4863
Fragments arranged by size:
4863 1800 281
AleI CACnn'nnGTG
Cuts at: 2924 3118 2924
Size: 194 6750
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 763 1414 3481 3665 763
Size: 651 2067 184 4042
Fragments arranged by size:
4042 2067 651 184
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2551 2583 2551
Size: 32 6912
AlwNI CAG_nnn'CTG
Cuts at: 1382 1651 2478 2511 2681 3804 4249 5687 1382
Size: 269 827 33 170 1123 445 1438 2639
Fragments arranged by size:
2639 1438 1123 827 445 269 170 33
ApaI G_GGCC'C
Cuts at: 1131 1310 1131
Size: 179 6765
ApaLI G'TGCA_C
Cuts at: 5782 5782
Size: 6944
ApoI r'AATT_y
Cuts at: 479 1596 2863 3698 3948 6297 6553 479
Size: 1117 1267 835 250 2349 256 870
Fragments arranged by size:
2349 1267 1117 870 835 256 250
AseI AT'TA_AT
Cuts at: 20 20
Size: 6944
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6944
AvaI C'yCGr_G
Cuts at: 175 367 664 1546 2997 3964 175
Size: 192 297 882 1451 967 3155
Fragments arranged by size:
3155 1451 967 882 297 192
AvrII C'CTAG_G
Cuts at: 1117 1117
Size: 6944
BamHI G'GATC_C
Cuts at: 442 1665 3969 442
Size: 1223 2304 3417
Fragments arranged by size:
3417 2304 1223
BanI G'GyrC_C
Cuts at: 135 358 619 798 960 2048 2160 2369 3001
Size: 223 261 179 162 1088 112 209 632
Cuts at: 3001 3776 3960 4374 4622 4657 135
Size: 775 184 414 248 35 2422
Fragments arranged by size:
2422 1088 775 632 414 261 248 223
209 184 179 162 112 35
BanII G_rGCy'C
Cuts at: 576 592 901 1039 1131 1310 1528 1537 1546
Size: 16 309 138 92 179 218 9 9
Cuts at: 1546 1849 2040 2297 3017 3958 4988 576
Size: 303 191 257 720 941 1030 2532
Fragments arranged by size:
2532 1030 941 720 309 303 257 218
191 179 138 92 16 9 9
BbeI G_GCGC'C
Cuts at: 2052 3005 4378 4626 2052
Size: 953 1373 248 4370
Fragments arranged by size:
4370 1373 953 248
BbsI GAAGACnn'nnnn_
Cuts at: 1059 1928 1958 2009 2351 3629 1059
Size: 869 30 51 342 1278 4374
Fragments arranged by size:
4374 1278 869 342 51 30
BbvCI CC'TCA_GC
Cuts at: 3547 3547
Size: 6944
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3621 3655 3621
Size: 34 6910
BciVI GTATCCnnnnn_n'
Cuts at: 4842 5898 6944 4842
Size: 1056 1046 4842
Fragments arranged by size:
4842 1056 1046
BclI T'GATC_A
Cuts at: 2411 3242 4464 2411
Size: 831 1222 4891
Fragments arranged by size:
4891 1222 831
BfrBI ATG'CAT
Cuts at: 1248 1248
Size: 6944
BglI GCCn_nnn'nGGC
Cuts at: 278 278
Size: 6944
BglII A'GATC_T
Cuts at: 3128 4459 6496 3128
Size: 1331 2037 3576
Fragments arranged by size:
3576 2037 1331
BlpI GC'TnA_GC
Cuts at: 1290 4056 1290
Size: 2766 4178
Bme1580I G_kGCm'C
Cuts at: 138 363 624 646 803 1131 1310 1587 3781
Size: 225 261 22 157 328 179 277 2194
Cuts at: 3781 4569 4662 5786 138
Size: 788 93 1124 1296
Fragments arranged by size:
2194 1296 1124 788 328 277 261 225
179 157 93 22
BmrI ACTGGGnnnn_n'
Cuts at: 2488 2630 4156 4237 4572 6716 2488
Size: 142 1526 81 335 2144 2716
Fragments arranged by size:
2716 2144 1526 335 142 81
BmtI G_CTAG'C
Cuts at: 2830 2830
Size: 6944
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5311 5311
Size: 6944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1050 1122 1160 1188 1800 2000 2607 3031
Size: 884 72 38 28 612 200 607 424
Cuts at: 3031 3286 166
Size: 255 3824
Fragments arranged by size:
3824 884 612 607 424 255 200 72
38 28
Bpu10I CC'TnA_GC
Cuts at: 597 976 1478 3547 597
Size: 379 502 2069 3994
Fragments arranged by size:
3994 2069 502 379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2911 3148 4120 4999 5713 6011 2911
Size: 237 972 879 714 298 3844
Fragments arranged by size:
3844 972 879 714 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 6648 6648
Size: 6944
BsaAI yAC'GTr
Cuts at: 4297 4297
Size: 6944
BsaBI GATnn'nnATC
Cuts at: 6 3088 4458 6495 6
Size: 3082 1370 2037 455
Fragments arranged by size:
3082 2037 1370 455
BsaHI Gr'CG_yC
Cuts at: 221 2049 2244 3002 4375 4623 5325 6821 221
Size: 1828 195 758 1373 248 702 1496 344
Fragments arranged by size:
1828 1496 1373 758 702 344 248 195
BsaWI w'CCGG_w
Cuts at: 431 3932 4133 4654 5332 5743 5890 6164 431
Size: 3501 201 521 678 411 147 274 1211
Fragments arranged by size:
3501 1211 678 521 411 274 201 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1098 1128 1174 1204 1098
Size: 30 46 30 6838
Fragments arranged by size:
6838 46 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 393 873 1047 1119 1771 1851 2341 2656 2668
Size: 480 174 72 652 80 490 315 12
Cuts at: 2668 393
Size: 4669
Fragments arranged by size:
4669 652 490 480 315 174 80 72
12
BseYI C'CCAG_C
Cuts at: 312 3311 3851 4369 5127 5792 6200 312
Size: 2999 540 518 758 665 408 1056
Fragments arranged by size:
2999 1056 758 665 540 518 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 1251 1368 3142 3671 3790 221
Size: 1030 117 1774 529 119 3375
Fragments arranged by size:
3375 1774 1030 529 119 117
BsiEI CG_ry'CG
Cuts at: 104 4486 4532 5762 104
Size: 4382 46 1230 1286
Fragments arranged by size:
4382 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 697 871 901 1213 2218 3017 3958 4736 4926
Size: 174 30 312 1005 799 941 778 190
Cuts at: 4926 5786 697
Size: 860 1855
Fragments arranged by size:
1855 1005 941 860 799 778 312 190
174 30
BsmI GAATG_Cn'
Cuts at: 2016 2016
Size: 6944
BsmBI CGTCTCn'nnnn_
Cuts at: 1206 1848 1206
Size: 642 6302
Bsp1286I G_dGCh'C
Cuts at: 138 363 576 592 624 646 697 803 871
Size: 225 213 16 32 22 51 106 68
Cuts at: 871 901 1039 1131 1213 1310 1528 1537 1546
Size: 30 138 92 82 97 218 9 9
Cuts at: 1546 1587 1849 2040 2218 2297 3017 3781 3958
Size: 41 262 191 178 79 720 764 177
Cuts at: 3958 4569 4662 4736 4926 4988 5786 138
Size: 611 93 74 190 62 798 1296
Fragments arranged by size:
1296 798 764 720 611 262 225 218
213 191 190 178 177 138 106 97
93 92 82 79 74 68 62 51
41 32 30 22 16 9 9
BspEI T'CCGG_A
Cuts at: 431 3932 431
Size: 3501 3443
BspHI T'CATG_A
Cuts at: 3026 5376 3026
Size: 2350 4594
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1273 2381 3285 3458 3849 3994 4208 4510 4891
Size: 1108 904 173 391 145 214 302 381
Cuts at: 4891 1273
Size: 3326
Fragments arranged by size:
3326 1108 904 391 381 302 214 173
145
BsrBI CCG'CTC
Cuts at: 50 372 704 2739 5236 5290 6774 6939 50
Size: 322 332 2035 2497 54 1484 165 55
Fragments arranged by size:
2497 2035 1484 332 322 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1089 4856 1089
Size: 3767 3177
BsrFI r'CCGG_y
Cuts at: 1172 1732 2127 2246 3482 4942 5123 1172
Size: 560 395 119 1236 1460 181 2993
Fragments arranged by size:
2993 1460 1236 560 395 181 119
BsrGI T'GTAC_A
Cuts at: 2744 2744
Size: 6944
BssSI C'ACGA_G
Cuts at: 820 5215 5923 820
Size: 4395 708 1841
Fragments arranged by size:
4395 1841 708
BstAPI GCAn_nnn'nTGC
Cuts at: 619 1558 2700 4321 619
Size: 939 1142 1621 3242
Fragments arranged by size:
3242 1621 1142 939
BstBI TT'CG_AA
Cuts at: 5305 5305
Size: 6944
BstEII G'GTnAC_C
Cuts at: 231 231
Size: 6944
BstXI CCAn_nnnn'nTGG
Cuts at: 390 638 1006 2229 390
Size: 248 368 1223 5105
Fragments arranged by size:
5105 1223 368 248
BstYI r'GATC_y
Cuts at: 1 442 1665 2935 3128 3969 4436 4459 4794
Size: 441 1223 1270 193 841 467 23 335
Cuts at: 4794 5040 5346 5358 5444 6496 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1270 1223 1052 841 467 449 441 335
306 246 193 86 23 12
BstZ17I GTA'TAC
Cuts at: 3170 3170
Size: 6944
Bsu36I CC'TnA_GG
Cuts at: 625 937 2758 625
Size: 312 1821 4811
Fragments arranged by size:
4811 1821 312
BtgI C'CryG_G
Cuts at: 383 1271 2462 3782 383
Size: 888 1191 1320 3545
Fragments arranged by size:
3545 1320 1191 888
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3075 4894 5075 5238 3075
Size: 1819 181 163 4781
Fragments arranged by size:
4781 1819 181 163
BtsI GCAGTG_nn'
Cuts at: 158 1256 1325 2553 4187 158
Size: 1098 69 1228 1634 2915
Fragments arranged by size:
2915 1634 1228 1098 69
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2820 2855 2820
Size: 35 6909
DraI TTT'AAA
Cuts at: 3943 3943
Size: 6944
DraIII CAC_nnn'GTG
Cuts at: 1687 2160 1687
Size: 473 6471
DrdI GACnn_nn'nnGTC
Cuts at: 4650 5994 4650
Size: 1344 5600
EaeI y'GGCC_r
Cuts at: 792 1858 1870 1887 1895 2396 3152 4529 4703
Size: 1066 12 17 8 501 756 1377 174
Cuts at: 4703 5094 5121 6466 792
Size: 391 27 1345 1270
Fragments arranged by size:
1377 1345 1270 1066 756 501 391 174
27 17 12 8
EagI C'GGCC_G
Cuts at: 4529 4529
Size: 6944
EarI CTCTTCn'nnn_
Cuts at: 343 889 1619 2600 3904 4967 5177 343
Size: 546 730 981 1304 1063 210 2110
Fragments arranged by size:
2110 1304 1063 981 730 546 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3889 5152 5894 6040 6754 3889
Size: 1263 742 146 714 4079
Fragments arranged by size:
4079 1263 742 714 146
EcoICRI GAG'CTC
Cuts at: 899 3015 3956 899
Size: 2116 941 3887
Fragments arranged by size:
3887 2116 941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 478 910 1050 1122 1144 1160 1188 1800
Size: 312 432 140 72 22 16 28 612
Cuts at: 1800 1940 2000 2021 2261 2319 2607 3031 3286
Size: 140 60 21 240 58 288 424 255
Cuts at: 3286 4769 5201 5554 6216 166
Size: 1483 432 353 662 894
Fragments arranged by size:
1483 894 662 612 432 432 424 353
312 288 255 240 140 140 72 60
58 28 22 21 16
EcoNI CCTnn'n_nnAGG
Cuts at: 1104 2353 3915 1104
Size: 1249 1562 4133
Fragments arranged by size:
4133 1562 1249
EcoO109I rG'GnC_Cy
Cuts at: 184 828 971 1127 1128 1306 1307 1648 1704
Size: 644 143 156 1 178 1 341 56
Cuts at: 1704 1968 3447 3801 4083 184
Size: 264 1479 354 282 3045
Fragments arranged by size:
3045 1479 644 354 341 282 264 178
156 143 56 1 1
EcoRI G'AATT_C
Cuts at: 479 1596 3948 479
Size: 1117 2352 3475
Fragments arranged by size:
3475 2352 1117
EcoRV GAT'ATC
Cuts at: 3327 3327
Size: 6944
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 918 2003 4963 918
Size: 1085 2960 2899
Fragments arranged by size:
2960 2899 1085
FspI TGC'GCA
Cuts at: 2436 2822 3457 4725 2436
Size: 386 635 1268 4655
Fragments arranged by size:
4655 1268 635 386
HaeII r_GCGC'y
Cuts at: 563 2052 3005 3825 4378 4626 5856 6627 563
Size: 1489 953 820 553 248 1230 771 880
Fragments arranged by size:
1489 1230 953 880 820 771 553 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1098 1130 1174 1206 3311 3325 3343 3357 4786
Size: 32 44 32 2105 14 18 14 1429
Cuts at: 4786 4816 4818 4848 1098
Size: 30 2 30 3194
Fragments arranged by size:
3194 2105 1429 44 32 32 30 30
18 14 14 2
HincII GTy'rAC
Cuts at: 1466 3983 1466
Size: 2517 4427
HindIII A'AGCT_T
Cuts at: 319 1003 319
Size: 684 6260
KasI G'GCGC_C
Cuts at: 2048 3001 4374 4622 2048
Size: 953 1373 248 4370
Fragments arranged by size:
4370 1373 953 248
KpnI G_GTAC'C
Cuts at: 964 2373 3964 964
Size: 1409 1591 3944
Fragments arranged by size:
3944 1591 1409
MluI A'CGCG_T
Cuts at: 6377 6377
Size: 6944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 325 683 2210 2291 2546 2594 2808 5703 5887
Size: 358 1527 81 255 48 214 2895 184
Cuts at: 5887 325
Size: 1382
Fragments arranged by size:
2895 1527 1382 358 255 214 184 81
48
MscI TGG'CCA
Cuts at: 794 1897 2398 4705 794
Size: 1103 501 2307 3033
Fragments arranged by size:
3033 2307 1103 501
MslI CAynn'nnrTG
Cuts at: 1688 1723 2059 2806 2924 3118 3832 4933 5060
Size: 35 336 747 118 194 714 1101 127
Cuts at: 5060 1688
Size: 3572
Fragments arranged by size:
3572 1101 747 714 336 194 127 118
35
MspA1I CmG'CkG
Cuts at: 690 1565 1893 2394 3144 3150 3402 3429 4055
Size: 875 328 501 750 6 252 27 626
Cuts at: 4055 4369 4729 5513 5758 690
Size: 314 360 784 245 1876
Fragments arranged by size:
1876 875 784 750 626 501 360 328
314 252 245 27 6
NaeI GCC'GGC
Cuts at: 1174 1734 2248 3484 5125 1174
Size: 560 514 1236 1641 2993
Fragments arranged by size:
2993 1641 1236 560 514
NarI GG'CG_CC
Cuts at: 2049 3002 4375 4623 2049
Size: 953 1373 248 4370
Fragments arranged by size:
4370 1373 953 248
NcoI C'CATG_G
Cuts at: 383 2462 383
Size: 2079 4865
NdeI CA'TA_TG
Cuts at: 3213 3213
Size: 6944
NgoMIV G'CCGG_C
Cuts at: 1172 1732 2246 3482 5123 1172
Size: 560 514 1236 1641 2993
Fragments arranged by size:
2993 1641 1236 560 514
NheI G'CTAG_C
Cuts at: 2826 2826
Size: 6944
NsiI A_TGCA'T
Cuts at: 1250 1250
Size: 6944
NspI r_CATG'y
Cuts at: 617 3468 3997 4497 6100 617
Size: 2851 529 500 1603 1461
Fragments arranged by size:
2851 1603 1461 529 500
PasI CC'CwG_GG
Cuts at: 131 131
Size: 6944
PciI A'CATG_T
Cuts at: 6096 6096
Size: 6944
PflMI CCAn_nnn'nTGG
Cuts at: 384 616 1028 1138 1453 2478 2511 3429 384
Size: 232 412 110 315 1025 33 918 3899
Fragments arranged by size:
3899 1025 918 412 315 232 110 33
PfoI T'CCnGG_A
Cuts at: 1204 1415 2717 1204
Size: 211 1302 5431
Fragments arranged by size:
5431 1302 211
PmeI GTTT'AAAC
Cuts at: 3943 3943
Size: 6944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5390 5422 5390
Size: 32 6912
PpuMI rG'GwC_Cy
Cuts at: 828 971 1704 1968 3447 828
Size: 143 733 264 1479 4325
Fragments arranged by size:
4325 1479 733 264 143
PshAI GACnn'nnGTC
Cuts at: 2710 2710
Size: 6944
PspOMI G'GGCC_C
Cuts at: 1127 1306 1127
Size: 179 6765
PspXI vC'TCGA_Gb
Cuts at: 664 664
Size: 6944
PstI C_TGCA'G
Cuts at: 271 1938 2378 3077 3149 3644 3991 4676 271
Size: 1667 440 699 72 495 347 685 2539
Fragments arranged by size:
2539 1667 699 685 495 440 347 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6944
PvuII CAG'CTG
Cuts at: 1565 1893 2394 3150 3429 4369 4729 1565
Size: 328 501 756 279 940 360 3780
Fragments arranged by size:
3780 940 756 501 360 328 279
RsrII CG'GwC_CG
Cuts at: 5139 5139
Size: 6944
SacI G_AGCT'C
Cuts at: 901 3017 3958 901
Size: 2116 941 3887
Fragments arranged by size:
3887 2116 941
SalI G'TCGA_C
Cuts at: 3981 3981
Size: 6944
SanDI GG'GwC_CC
Cuts at: 828 828
Size: 6944
SapI GCTCTTCn'nnn_
Cuts at: 889 1619 2600 3904 4967 5177 889
Size: 730 981 1304 1063 210 2656
Fragments arranged by size:
2656 1304 1063 981 730 210
SbfI CC_TGCA'GG
Cuts at: 3991 3991
Size: 6944
ScaI AGT'ACT
Cuts at: 2983 6461 2983
Size: 3478 3466
SexAI A'CCwGG_T
Cuts at: 2156 2156
Size: 6944
SfcI C'TryA_G
Cuts at: 33 267 852 1934 2374 3073 3145 3640 3987
Size: 234 585 1082 440 699 72 495 347
Cuts at: 3987 4672 5640 5831 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 1082 968 699 685 585 495 440
347 234 191 72
SfoI GGC'GCC
Cuts at: 2050 3003 4376 4624 2050
Size: 953 1373 248 4370
Fragments arranged by size:
4370 1373 953 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6944
SgrAI Cr'CCGG_yG
Cuts at: 2246 2246
Size: 6944
SmaI CCC'GGG
Cuts at: 1548 2999 3966 1548
Size: 1451 967 4526
Fragments arranged by size:
4526 1451 967
SmlI C'TyrA_G
Cuts at: 664 2890 2922 3163 4099 5014 5728 5990 664
Size: 2226 32 241 936 915 714 262 1618
Fragments arranged by size:
2226 1618 936 915 714 262 241 32
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6944
SphI G_CATG'C
Cuts at: 3997 4497 3997
Size: 500 6444
StuI AGG'CCT
Cuts at: 1866 3646 1866
Size: 1780 5164
StyI C'CwwG_G
Cuts at: 383 1049 1117 1861 2462 4078 4432 383
Size: 666 68 744 601 1616 354 2895
Fragments arranged by size:
2895 1616 744 666 601 354 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 307 792 1527 3350 4527 4660 5119 5328 307
Size: 485 735 1823 1177 133 459 209 1923
Fragments arranged by size:
1923 1823 1177 735 485 459 209 133
TatI w'GTAC_w
Cuts at: 2744 2900 2981 3041 3470 6459 2744
Size: 156 81 60 429 2989 3229
Fragments arranged by size:
3229 2989 429 156 81 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2521 2553 6461 6493 2521
Size: 32 3908 32 2972
Fragments arranged by size:
3908 2972 32 32
Tth111I GACn'n_nGTC
Cuts at: 1063 4741 1063
Size: 3678 3266
XbaI T'CTAG_A
Cuts at: 3975 3975
Size: 6944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 390 3228 4439 390
Size: 2838 1211 2895
Fragments arranged by size:
2895 2838 1211
XhoI C'TCGA_G
Cuts at: 664 664
Size: 6944
XmaI C'CCGG_G
Cuts at: 1546 2997 3964 1546
Size: 1451 967 4526
Fragments arranged by size:
4526 1451 967
XmnI GAAnn'nnTTC
Cuts at: 3350 3350
Size: 6944
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AscI BaeI BmgBI BsiWI
BssHII ClaI FseI FspAI HpaI MfeI NotI NruI
PacI PmlI PsiI PsrI SacII SfiI SnaBI SrfI
SspI SwaI ZraI