Restriction map for
pF1KF0107
(Circular) (Six-base) MAPSORT of: pF1KF0107.seq Check: 6514 from: 1 to: 7046
pF1KF0107 7046 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1002 1995 2252 1002
Size: 993 257 5796
Fragments arranged by size:
5796 993 257
AccI GT'mk_AC
Cuts at: 959 1535 4084 959
Size: 576 2549 3921
Fragments arranged by size:
3921 2549 576
Acc65I G'GTAC_C
Cuts at: 4062 4062
Size: 7046
AclI AA'CG_TT
Cuts at: 3539 6886 3539
Size: 3347 3699
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 1567 2167 2218 3535 4871 5303 5656 6318
Size: 1370 600 51 1317 1336 432 353 662
Cuts at: 6318 197
Size: 925
Fragments arranged by size:
1370 1336 1317 925 662 600 432 353
51
AflII C'TTAA_G
Cuts at: 1474 1917 1957 1474
Size: 443 40 6563
Fragments arranged by size:
6563 443 40
AflIII A'CryG_T
Cuts at: 695 2906 2984 4398 6198 6479 695
Size: 2211 78 1414 1800 281 1262
Fragments arranged by size:
2211 1800 1414 1262 281 78
AleI CACnn'nnGTG
Cuts at: 251 635 2221 2491 4037 251
Size: 384 1586 270 1546 3260
Fragments arranged by size:
3260 1586 1546 384 270
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 7014
AlwNI CAG_nnn'CTG
Cuts at: 4351 5789 4351
Size: 1438 5608
ApaLI G'TGCA_C
Cuts at: 244 1982 2367 5884 244
Size: 1738 385 3517 1406
Fragments arranged by size:
3517 1738 1406 385
ApoI r'AATT_y
Cuts at: 1806 2122 3150 3325 4050 6399 6655 1806
Size: 316 1028 175 725 2349 256 2197
Fragments arranged by size:
2349 2197 1028 725 316 256 175
AseI AT'TA_AT
Cuts at: 20 308 20
Size: 288 6758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7046
AvaI C'yCGr_G
Cuts at: 1296 4066 1296
Size: 2770 4276
AvrII C'CTAG_G
Cuts at: 2964 2964
Size: 7046
BamHI G'GATC_C
Cuts at: 4071 4071
Size: 7046
BanI G'GyrC_C
Cuts at: 2485 3868 4062 4476 4724 4759 2485
Size: 1383 194 414 248 35 4772
Fragments arranged by size:
4772 1383 414 248 194 35
BanII G_rGCy'C
Cuts at: 974 2882 3599 4060 5090 974
Size: 1908 717 461 1030 2930
Fragments arranged by size:
2930 1908 1030 717 461
BbeI G_GCGC'C
Cuts at: 4480 4728 4480
Size: 248 6798
BbsI GAAGACnn'nnnn_
Cuts at: 173 221 239 890 2259 2744 3523 3691 3959
Size: 48 18 651 1369 485 779 168 268
Cuts at: 3959 173
Size: 3260
Fragments arranged by size:
3260 1369 779 651 485 268 168 48
18
BbvCI CC'TCA_GC
Cuts at: 496 496
Size: 7046
BciVI GTATCCnnnnn_n'
Cuts at: 4944 6000 7046 4944
Size: 1056 1046 4944
Fragments arranged by size:
4944 1056 1046
BclI T'GATC_A
Cuts at: 666 2171 4566 666
Size: 1505 2395 3146
Fragments arranged by size:
3146 2395 1505
BfrBI ATG'CAT
Cuts at: 1642 3698 1642
Size: 2056 4990
BglII A'GATC_T
Cuts at: 3342 3959 4561 6598 3342
Size: 617 602 2037 3790
Fragments arranged by size:
3790 2037 617 602
BlpI GC'TnA_GC
Cuts at: 283 2876 4158 283
Size: 2593 1282 3171
Fragments arranged by size:
3171 2593 1282
Bme1580I G_kGCm'C
Cuts at: 248 1986 2371 2992 4671 4764 5888 248
Size: 1738 385 621 1679 93 1124 1406
Fragments arranged by size:
1738 1679 1406 1124 621 385 93
BmgBI CAC'GTC
Cuts at: 698 1993 3288 698
Size: 1295 1295 4456
Fragments arranged by size:
4456 1295 1295
BmrI ACTGGGnnnn_n'
Cuts at: 2391 3663 4258 4339 4674 6818 2391
Size: 1272 595 81 335 2144 2619
Fragments arranged by size:
2619 2144 1272 595 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 964 1005 1283 5413 964
Size: 41 278 4130 2597
Fragments arranged by size:
4130 2597 278 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 934 1166 1730 2021 2867 3082 3550 3811
Size: 684 232 564 291 846 215 468 261
Cuts at: 3811 250
Size: 3485
Fragments arranged by size:
3485 846 684 564 468 291 261 232
215
Bpu10I CC'TnA_GC
Cuts at: 496 1493 496
Size: 997 6049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3165 4222 5101 5815 6113 3165
Size: 1057 879 714 298 4098
Fragments arranged by size:
4098 1057 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 979 1748 2822 6750 979
Size: 769 1074 3928 1275
Fragments arranged by size:
3928 1275 1074 769
BsaAI yAC'GTr
Cuts at: 4399 4399
Size: 7046
BsaBI GATnn'nnATC
Cuts at: 6 4560 6597 6
Size: 4554 2037 455
Fragments arranged by size:
4554 2037 455
BsaHI Gr'CG_yC
Cuts at: 4477 4725 5427 6923 4477
Size: 248 702 1496 4600
Fragments arranged by size:
4600 1496 702 248
BsaWI w'CCGG_w
Cuts at: 805 1110 4235 4756 5434 5845 5992 6266 805
Size: 305 3125 521 678 411 147 274 1585
Fragments arranged by size:
3125 1585 678 521 411 305 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 323 353 323
Size: 30 7016
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 997 3124 238
Size: 759 2127 4160
Fragments arranged by size:
4160 2127 759
BseYI C'CCAG_C
Cuts at: 4471 5229 5894 6302 4471
Size: 758 665 408 5215
Fragments arranged by size:
5215 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 870 1973 2262 2680 870
Size: 1103 289 418 5236
Fragments arranged by size:
5236 1103 418 289
BsiEI CG_ry'CG
Cuts at: 104 4588 4634 5864 104
Size: 4484 46 1230 1286
Fragments arranged by size:
4484 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 248 974 1986 2371 2882 2914 3599 3848 4060
Size: 726 1012 385 511 32 685 249 212
Cuts at: 4060 4838 5028 5888 248
Size: 778 190 860 1406
Fragments arranged by size:
1406 1012 860 778 726 685 511 385
249 212 190 32
BsmI GAATG_Cn'
Cuts at: 925 1642 3790 925
Size: 717 2148 4181
Fragments arranged by size:
4181 2148 717
Bsp1286I G_dGCh'C
Cuts at: 248 974 1986 2371 2882 2914 2992 3599 3848
Size: 726 1012 385 511 32 78 607 249
Cuts at: 3848 4060 4671 4764 4838 5028 5090 5888 248
Size: 212 611 93 74 190 62 798 1406
Fragments arranged by size:
1406 1012 798 726 611 607 511 385
249 212 190 93 78 74 62 32
BspHI T'CATG_A
Cuts at: 777 995 5478 777
Size: 218 4483 2345
Fragments arranged by size:
4483 2345 218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 762 1002 1995 2252 4096 4310 4612 4993 762
Size: 240 993 257 1844 214 302 381 2815
Fragments arranged by size:
2815 1844 993 381 302 257 240 214
BsrBI CCG'CTC
Cuts at: 50 943 1267 3006 5338 5392 6876 7041 50
Size: 893 324 1739 2332 54 1484 165 55
Fragments arranged by size:
2332 1739 1484 893 324 165 55 54
BsrDI GCAATG_nn'
Cuts at: 899 1656 2509 3123 4958 899
Size: 757 853 614 1835 2987
Fragments arranged by size:
2987 1835 853 757 614
BsrFI r'CCGG_y
Cuts at: 3388 5044 5225 3388
Size: 1656 181 5209
Fragments arranged by size:
5209 1656 181
BsrGI T'GTAC_A
Cuts at: 1082 1801 3970 1082
Size: 719 2169 4158
Fragments arranged by size:
4158 2169 719
BssSI C'ACGA_G
Cuts at: 967 3598 5317 6025 967
Size: 2631 1719 708 1988
Fragments arranged by size:
2631 1988 1719 708
BstAPI GCAn_nnn'nTGC
Cuts at: 557 1621 2285 2375 2468 2688 2909 3794 4423
Size: 1064 664 90 93 220 221 885 629
Cuts at: 4423 557
Size: 3180
Fragments arranged by size:
3180 1064 885 664 629 221 220 93
90
BstBI TT'CG_AA
Cuts at: 2612 3854 5407 2612
Size: 1242 1553 4251
Fragments arranged by size:
4251 1553 1242
BstYI r'GATC_y
Cuts at: 1 136 677 3342 3959 4071 4538 4561 4896
Size: 135 541 2665 617 112 467 23 335
Cuts at: 4896 5142 5448 5460 5546 6598 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2665 1052 617 541 467 449 335 306
246 135 112 86 23 12
BtgI C'CryG_G
Cuts at: 408 732 1214 3219 3942 408
Size: 324 482 2005 723 3512
Fragments arranged by size:
3512 2005 723 482 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4996 5177 5340 4996
Size: 181 163 6702
Fragments arranged by size:
6702 181 163
BtsI GCAGTG_nn'
Cuts at: 630 642 4044 4289 630
Size: 12 3402 245 3387
Fragments arranged by size:
3402 3387 245 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1199 1234 1199
Size: 35 7011
DraI TTT'AAA
Cuts at: 4045 4045
Size: 7046
DrdI GACnn_nn'nnGTC
Cuts at: 696 2308 4752 6096 696
Size: 1612 2444 1344 1646
Fragments arranged by size:
2444 1646 1612 1344
EaeI y'GGCC_r
Cuts at: 364 1937 4631 4805 5196 5223 6568 364
Size: 1573 2694 174 391 27 1345 842
Fragments arranged by size:
2694 1573 1345 842 391 174 27
EagI C'GGCC_G
Cuts at: 4631 4631
Size: 7046
EarI CTCTTCn'nnn_
Cuts at: 199 979 1252 1586 1688 1689 3101 5069 5279
Size: 780 273 334 102 1 1412 1968 210
Cuts at: 5279 199
Size: 1966
Fragments arranged by size:
1968 1966 1412 780 334 273 210 102
1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5254 5996 6142 6856 5254
Size: 742 146 714 5444
Fragments arranged by size:
5444 742 714 146
EcoICRI GAG'CTC
Cuts at: 972 2880 3597 4058 972
Size: 1908 717 461 3960
Fragments arranged by size:
3960 1908 717 461
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 250 934 1166 1567 1730 2021 2167 2218
Size: 53 684 232 401 163 291 146 51
Cuts at: 2218 2867 3082 3535 3550 3811 4871 5303 5656
Size: 649 215 453 15 261 1060 432 353
Cuts at: 5656 6318 197
Size: 662 925
Fragments arranged by size:
1060 925 684 662 649 453 432 401
353 291 261 232 215 163 146 53
51 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2287 2287
Size: 7046
EcoO109I rG'GnC_Cy
Cuts at: 1440 1497 1913 2968 4185 1440
Size: 57 416 1055 1217 4301
Fragments arranged by size:
4301 1217 1055 416 57
EcoRI G'AATT_C
Cuts at: 2122 3150 3325 4050 2122
Size: 1028 175 725 5118
Fragments arranged by size:
5118 1028 725 175
EcoRV GAT'ATC
Cuts at: 291 3360 291
Size: 3069 3977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 699 4011 5065 699
Size: 3312 1054 2680
Fragments arranged by size:
3312 2680 1054
FspI TGC'GCA
Cuts at: 3309 4827 3309
Size: 1518 5528
FspAI rTGC'GCAy
Cuts at: 3309 3309
Size: 7046
HaeII r_GCGC'y
Cuts at: 3416 4480 4728 5958 6729 3416
Size: 1064 248 1230 771 3733
Fragments arranged by size:
3733 1230 1064 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1936 1968 1994 2026 2091 2123 4888 4918 4920
Size: 32 26 32 65 32 2765 30 2
Cuts at: 4920 4950 1936
Size: 30 4032
Fragments arranged by size:
4032 2765 65 32 32 32 30 30
26 2
HincII GTy'rAC
Cuts at: 319 1566 2745 4085 319
Size: 1247 1179 1340 3280
Fragments arranged by size:
3280 1340 1247 1179
KasI G'GCGC_C
Cuts at: 4476 4724 4476
Size: 248 6798
KpnI G_GTAC'C
Cuts at: 4066 4066
Size: 7046
MluI A'CGCG_T
Cuts at: 6479 6479
Size: 7046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 209 376 725 1214 3653 5805 5989 209
Size: 167 349 489 2439 2152 184 1266
Fragments arranged by size:
2439 2152 1266 489 349 184 167
MscI TGG'CCA
Cuts at: 366 1939 4807 366
Size: 1573 2868 2605
Fragments arranged by size:
2868 2605 1573
MslI CAynn'nnrTG
Cuts at: 251 462 635 714 2221 2491 4037 5035 5162
Size: 211 173 79 1507 270 1546 998 127
Cuts at: 5162 251
Size: 2135
Fragments arranged by size:
2135 1546 1507 998 270 211 173 127
79
MspA1I CmG'CkG
Cuts at: 251 260 1325 4157 4471 4831 5615 5860 251
Size: 9 1065 2832 314 360 784 245 1437
Fragments arranged by size:
2832 1437 1065 784 360 314 245 9
NaeI GCC'GGC
Cuts at: 3390 5227 3390
Size: 1837 5209
NarI GG'CG_CC
Cuts at: 4477 4725 4477
Size: 248 6798
NcoI C'CATG_G
Cuts at: 408 1214 3219 3942 408
Size: 806 2005 723 3512
Fragments arranged by size:
3512 2005 806 723
NdeI CA'TA_TG
Cuts at: 2951 3375 2951
Size: 424 6622
NgoMIV G'CCGG_C
Cuts at: 3388 5225 3388
Size: 1837 5209
NsiI A_TGCA'T
Cuts at: 1644 3700 1644
Size: 2056 4990
NspI r_CATG'y
Cuts at: 771 2155 2256 2988 3186 3237 4099 4599 6202
Size: 1384 101 732 198 51 862 500 1603
Cuts at: 6202 771
Size: 1615
Fragments arranged by size:
1615 1603 1384 862 732 500 198 101
51
PasI CC'CwG_GG
Cuts at: 448 448
Size: 7046
PciI A'CATG_T
Cuts at: 2984 6198 2984
Size: 3214 3832
PflMI CCAn_nnn'nTGG
Cuts at: 1753 1753
Size: 7046
PfoI T'CCnGG_A
Cuts at: 811 878 2942 811
Size: 67 2064 4915
Fragments arranged by size:
4915 2064 67
PmeI GTTT'AAAC
Cuts at: 4045 4045
Size: 7046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5492 5524 5492
Size: 32 7014
PpuMI rG'GwC_Cy
Cuts at: 1440 1497 1440
Size: 57 6989
PshAI GACnn'nnGTC
Cuts at: 1502 1502
Size: 7046
PspXI vC'TCGA_Gb
Cuts at: 1296 1296
Size: 7046
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1536 1568 1536
Size: 32 7014
PstI C_TGCA'G
Cuts at: 562 637 1373 1626 2684 2813 3261 3799 4013
Size: 75 736 253 1058 129 448 538 214
Cuts at: 4013 4093 4778 562
Size: 80 685 2830
Fragments arranged by size:
2830 1058 736 685 538 448 253 214
129 80 75
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7046
PvuII CAG'CTG
Cuts at: 251 260 1325 4471 4831 251
Size: 9 1065 3146 360 2466
Fragments arranged by size:
3146 2466 1065 360 9
RsrII CG'GwC_CG
Cuts at: 5241 5241
Size: 7046
SacI G_AGCT'C
Cuts at: 974 2882 3599 4060 974
Size: 1908 717 461 3960
Fragments arranged by size:
3960 1908 717 461
SalI G'TCGA_C
Cuts at: 4083 4083
Size: 7046
SapI GCTCTTCn'nnn_
Cuts at: 979 1688 5069 5279 979
Size: 709 3381 210 2746
Fragments arranged by size:
3381 2746 709 210
SbfI CC_TGCA'GG
Cuts at: 4093 4093
Size: 7046
ScaI AGT'ACT
Cuts at: 6563 6563
Size: 7046
SfcI C'TryA_G
Cuts at: 33 558 633 1320 1369 1622 2361 2547 2680
Size: 525 75 687 49 253 739 186 133
Cuts at: 2680 2809 3257 3795 4009 4089 4774 5742 5933
Size: 129 448 538 214 80 685 968 191
Cuts at: 5933 33
Size: 1146
Fragments arranged by size:
1146 968 739 687 685 538 525 448
253 214 191 186 133 129 80 75
49
SfoI GGC'GCC
Cuts at: 4478 4726 4478
Size: 248 6798
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7046
SmaI CCC'GGG
Cuts at: 4068 4068
Size: 7046
SmlI C'TyrA_G
Cuts at: 1296 1474 1917 1957 3144 4201 5116 5830 6092
Size: 178 443 40 1187 1057 915 714 262
Cuts at: 6092 1296
Size: 2250
Fragments arranged by size:
2250 1187 1057 915 714 443 262 178
40
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7046
SphI G_CATG'C
Cuts at: 3186 4099 4599 3186
Size: 913 500 5633
Fragments arranged by size:
5633 913 500
SspI AAT'ATT
Cuts at: 1817 1817
Size: 7046
StyI C'CwwG_G
Cuts at: 408 1214 1874 2442 2964 3203 3219 3942 4180
Size: 806 660 568 522 239 16 723 238
Cuts at: 4180 4534 408
Size: 354 2920
Fragments arranged by size:
2920 806 723 660 568 522 354 239
238 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 953 2640 4629 4762 5221 5430 953
Size: 1687 1989 133 459 209 2569
Fragments arranged by size:
2569 1989 1687 459 209 133
TatI w'GTAC_w
Cuts at: 1082 1801 2033 2531 3970 6561 1082
Size: 719 232 498 1439 2591 1567
Fragments arranged by size:
2591 1567 1439 719 498 232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2073 2105 6563 6595 2073
Size: 32 4458 32 2524
Fragments arranged by size:
4458 2524 32 32
Tth111I GACn'n_nGTC
Cuts at: 4843 4843
Size: 7046
XbaI T'CTAG_A
Cuts at: 4077 4077
Size: 7046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1197 3342 4541 1197
Size: 2145 1199 3702
Fragments arranged by size:
3702 2145 1199
XhoI C'TCGA_G
Cuts at: 1296 1296
Size: 7046
XmaI C'CCGG_G
Cuts at: 4066 4066
Size: 7046
XmnI GAAnn'nnTTC
Cuts at: 3991 3991
Size: 7046
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AlfI ApaI AscI BaeI
BcgI BglI BmtI BsiWI BsmBI BspEI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII FseI HindIII HpaI
MfeI NheI NotI NruI PacI PmlI PsiI PspOMI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI ZraI