Restriction map for
pF1KE9678
(Circular) (Six-base) MAPSORT of: pF1KE9678.seq Check: 5538 from: 1 to: 7364
pF1KE9678 7364 bp
With 182 enzymes: SgfI *
April 12, 2016 09:51 ..
AccI GT'mk_AC
Cuts at: 3452 3668 4402 3452
Size: 216 734 6414
Fragments arranged by size:
6414 734 216
Acc65I G'GTAC_C
Cuts at: 4380 4380
Size: 7364
AclI AA'CG_TT
Cuts at: 7204 7204
Size: 7364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 309 860 1294 2662 3929 4004 5189 5621
Size: 91 551 434 1368 1267 75 1185 432
Cuts at: 5621 6368 6636 218
Size: 747 268 946
Fragments arranged by size:
1368 1267 1185 946 747 551 434 432
268 91 75
AflII C'TTAA_G
Cuts at: 4336 4336
Size: 7364
AflIII A'CryG_T
Cuts at: 2825 3411 4195 4716 5820 6797 2825
Size: 586 784 521 1104 977 3392
Fragments arranged by size:
3392 1104 977 784 586 521
AgeI A'CCGG_T
Cuts at: 5752 5752
Size: 7364
AhdI GACnn_n'nnGTC
Cuts at: 1925 1925
Size: 7364
AleI CACnn'nnGTG
Cuts at: 1693 1693
Size: 7364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2754 2754
Size: 7364
AlwNI CAG_nnn'CTG
Cuts at: 1164 4669 6236 1164
Size: 3505 1567 2292
Fragments arranged by size:
3505 2292 1567
ApaLI G'TGCA_C
Cuts at: 6134 6134
Size: 7364
ApoI r'AATT_y
Cuts at: 199 526 927 976 1449 1467 1488 2004 2092
Size: 327 401 49 473 18 21 516 88
Cuts at: 2092 3687 4368 6717 6973 199
Size: 1595 681 2349 256 590
Fragments arranged by size:
2349 1595 681 590 516 473 401 327
256 88 49 21 18
AseI AT'TA_AT
Cuts at: 20 1252 2912 20
Size: 1232 1660 4472
Fragments arranged by size:
4472 1660 1232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7364
AvaI C'yCGr_G
Cuts at: 500 4384 500
Size: 3884 3480
AvrII C'CTAG_G
Cuts at: 1527 1527
Size: 7364
BamHI G'GATC_C
Cuts at: 4240 4389 4240
Size: 149 7215
BanI G'GyrC_C
Cuts at: 4380 4794 5042 5077 4380
Size: 414 248 35 6667
Fragments arranged by size:
6667 414 248 35
BanII G_rGCy'C
Cuts at: 2846 4052 4378 5408 2846
Size: 1206 326 1030 4802
Fragments arranged by size:
4802 1206 1030 326
BbeI G_GCGC'C
Cuts at: 4798 5046 4798
Size: 248 7116
BbsI GAAGACnn'nnnn_
Cuts at: 320 1366 1817 1921 2162 3584 3917 3992 320
Size: 1046 451 104 241 1422 333 75 3692
Fragments arranged by size:
3692 1422 1046 451 333 241 104 75
BbvCI CC'TCA_GC
Cuts at: 1911 4043 1911
Size: 2132 5232
BciVI GTATCCnnnnn_n'
Cuts at: 498 5262 6023 7364 498
Size: 4764 761 1341 498
Fragments arranged by size:
4764 1341 761 498
BclI T'GATC_A
Cuts at: 4884 4884
Size: 7364
BfrBI ATG'CAT
Cuts at: 1821 2998 4351 1821
Size: 1177 1353 4834
Fragments arranged by size:
4834 1353 1177
BglII A'GATC_T
Cuts at: 1125 2414 4879 6916 1125
Size: 1289 2465 2037 1573
Fragments arranged by size:
2465 2037 1573 1289
BlpI GC'TnA_GC
Cuts at: 4476 4476
Size: 7364
Bme1580I G_kGCm'C
Cuts at: 4989 5082 6138 4989
Size: 93 1056 6215
Fragments arranged by size:
6215 1056 93
BmgBI CAC'GTC
Cuts at: 2979 2979
Size: 7364
BmrI ACTGGGnnnn_n'
Cuts at: 1629 1815 4576 4657 4992 7136 1629
Size: 186 2761 81 335 2144 1857
Fragments arranged by size:
2761 2144 1857 335 186 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2860 5731 2860
Size: 2871 4493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 1004 2201 2344 2390 2467 2781 4212 371
Size: 633 1197 143 46 77 314 1431 3523
Fragments arranged by size:
3523 1431 1197 633 314 143 77 46
Bpu10I CC'TnA_GC
Cuts at: 863 1911 4043 863
Size: 1048 2132 4184
Fragments arranged by size:
4184 2132 1048
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 499 2274 3829 4250 4540 5419 5911 6209 499
Size: 1775 1555 421 290 879 492 298 1654
Fragments arranged by size:
1775 1654 1555 879 492 421 298 290
BsaI GGTCTCn'nnnn_
Cuts at: 7068 7068
Size: 7364
BsaAI yAC'GTr
Cuts at: 4717 4717
Size: 7364
BsaBI GATnn'nnATC
Cuts at: 6 420 817 4878 6915 6
Size: 414 397 4061 2037 455
Fragments arranged by size:
4061 2037 455 414 397
BsaHI Gr'CG_yC
Cuts at: 4795 5043 5745 7241 4795
Size: 248 702 1496 4918
Fragments arranged by size:
4918 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2580 2973 4553 5074 5752 6026 6173 6584 2580
Size: 393 1580 521 678 274 147 411 3360
Fragments arranged by size:
3360 1580 678 521 411 393 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2779 2809 3459 3489 2779
Size: 30 650 30 6654
Fragments arranged by size:
6654 650 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 732 4060 4161 732
Size: 3328 101 3935
Fragments arranged by size:
3935 3328 101
BseYI C'CCAG_C
Cuts at: 4789 5547 6124 6620 4789
Size: 758 577 496 5533
Fragments arranged by size:
5533 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 2917 2126
Size: 791 6573
BsiEI CG_ry'CG
Cuts at: 104 4906 4952 6160 104
Size: 4802 46 1208 1308
Fragments arranged by size:
4802 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1241 4052 4378 5156 5346 6138 1241
Size: 2811 326 778 190 792 2467
Fragments arranged by size:
2811 2467 792 778 326 190
BsmI GAATG_Cn'
Cuts at: 3217 4351 3217
Size: 1134 6230
Bsp1286I G_dGCh'C
Cuts at: 1241 2846 4052 4378 4989 5082 5156 5346 5408
Size: 1605 1206 326 611 93 74 190 62
Cuts at: 5408 6138 1241
Size: 730 2467
Fragments arranged by size:
2467 1605 1206 730 611 326 190 93
74 62
BspEI T'CCGG_A
Cuts at: 6584 6584
Size: 7364
BspHI T'CATG_A
Cuts at: 3093 6540 3093
Size: 3447 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4414 4628 4930 5311 4414
Size: 214 302 381 6467
Fragments arranged by size:
6467 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5656 5710 7194 7359 50
Size: 5606 54 1484 165 55
Fragments arranged by size:
5606 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 833 5276 833
Size: 4443 2921
BsrFI r'CCGG_y
Cuts at: 5362 5543 5752 5362
Size: 181 209 6974
Fragments arranged by size:
6974 209 181
BssSI C'ACGA_G
Cuts at: 5635 5993 5635
Size: 358 7006
BstAPI GCAn_nnn'nTGC
Cuts at: 4741 4741
Size: 7364
BstBI TT'CG_AA
Cuts at: 5725 5725
Size: 7364
BstXI CCAn_nnnn'nTGG
Cuts at: 3037 3037
Size: 7364
BstYI r'GATC_y
Cuts at: 1 1125 2414 3508 4240 4389 4856 4879 5214
Size: 1124 1289 1094 732 149 467 23 335
Cuts at: 5214 5460 6472 6558 6570 6916 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1289 1124 1094 1012 732 467 449 346
335 246 149 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2657 3878 2657
Size: 1221 6143
BtgI C'CryG_G
Cuts at: 107 2816 107
Size: 2709 4655
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 821 3959 5314 5495 5658 821
Size: 3138 1355 181 163 2527
Fragments arranged by size:
3138 2527 1355 181 163
BtsI GCAGTG_nn'
Cuts at: 650 1688 2143 3069 3702 4607 650
Size: 1038 455 926 633 905 3407
Fragments arranged by size:
3407 1038 926 905 633 455
ClaI AT'CG_AT
Cuts at: 821 821
Size: 7364
DraI TTT'AAA
Cuts at: 219 378 690 3546 3662 4363 219
Size: 159 312 2856 116 701 3220
Fragments arranged by size:
3220 2856 701 312 159 116
DraIII CAC_nnn'GTG
Cuts at: 2503 2503
Size: 7364
DrdI GACnn_nn'nnGTC
Cuts at: 5070 5928 5070
Size: 858 6506
EaeI y'GGCC_r
Cuts at: 1879 2597 4949 5123 5514 5541 6886 1879
Size: 718 2352 174 391 27 1345 2357
Fragments arranged by size:
2357 2352 1345 718 391 174 27
EagI C'GGCC_G
Cuts at: 4949 4949
Size: 7364
EarI CTCTTCn'nnn_
Cuts at: 1056 3045 3737 4105 5387 5597 1056
Size: 1989 692 368 1282 210 2823
Fragments arranged by size:
2823 1989 1282 692 368 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5572 5882 6028 7174 5572
Size: 310 146 1146 5762
Fragments arranged by size:
5762 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4050 4376 4050
Size: 326 7038
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 309 371 860 1004 1294 2201 2344 2390
Size: 91 62 489 144 290 907 143 46
Cuts at: 2390 2467 2662 2781 3929 4004 4212 5189 5621
Size: 77 195 119 1148 75 208 977 432
Cuts at: 5621 6368 6636 218
Size: 747 268 946
Fragments arranged by size:
1148 977 946 907 747 489 432 290
268 208 195 144 143 119 91 77
75 62 46
EcoNI CCTnn'n_nnAGG
Cuts at: 4134 4134
Size: 7364
EcoO109I rG'GnC_Cy
Cuts at: 1077 3418 4024 4503 1077
Size: 2341 606 479 3938
Fragments arranged by size:
3938 2341 606 479
EcoRI G'AATT_C
Cuts at: 1449 4368 1449
Size: 2919 4445
EcoRV GAT'ATC
Cuts at: 4208 4208
Size: 7364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 390 699 715 3687 5383 390
Size: 309 16 2972 1696 2371
Fragments arranged by size:
2972 2371 1696 309 16
FspI TGC'GCA
Cuts at: 5145 5145
Size: 7364
HaeII r_GCGC'y
Cuts at: 4798 5046 6068 7047 4798
Size: 248 1022 979 5115
Fragments arranged by size:
5115 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1117 1149 1911 1943 2249 2281 4290 4322 5206
Size: 32 762 32 306 32 2009 32 884
Cuts at: 5206 5236 5238 5268 1117
Size: 30 2 30 3213
Fragments arranged by size:
3213 2009 884 762 306 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3453 4403 3453
Size: 950 6414
HindIII A'AGCT_T
Cuts at: 3850 3850
Size: 7364
KasI G'GCGC_C
Cuts at: 4794 5042 4794
Size: 248 7116
KpnI G_GTAC'C
Cuts at: 4384 4384
Size: 7364
MfeI C'AATT_G
Cuts at: 2945 2945
Size: 7364
MluI A'CGCG_T
Cuts at: 6797 6797
Size: 7364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 6035 6219 6035
Size: 184 7180
MscI TGG'CCA
Cuts at: 5125 5125
Size: 7364
MslI CAynn'nnrTG
Cuts at: 851 1693 2768 3194 3284 5353 5480 851
Size: 842 1075 426 90 2069 127 2735
Fragments arranged by size:
2735 2069 1075 842 426 127 90
MspA1I CmG'CkG
Cuts at: 1317 1852 2640 3982 4042 4475 4789 5149 6162
Size: 535 788 1342 60 433 314 360 1013
Cuts at: 6162 6407 1317
Size: 245 2274
Fragments arranged by size:
2274 1342 1013 788 535 433 360 314
245 60
NaeI GCC'GGC
Cuts at: 5545 5545
Size: 7364
NarI GG'CG_CC
Cuts at: 4795 5043 4795
Size: 248 7116
NcoI C'CATG_G
Cuts at: 107 2816 107
Size: 2709 4655
NdeI CA'TA_TG
Cuts at: 2769 4347 2769
Size: 1578 5786
NgoMIV G'CCGG_C
Cuts at: 5543 5543
Size: 7364
NsiI A_TGCA'T
Cuts at: 1823 3000 4353 1823
Size: 1177 1353 4834
Fragments arranged by size:
4834 1353 1177
NspI r_CATG'y
Cuts at: 3415 4199 4417 4917 5824 3415
Size: 784 218 500 907 4955
Fragments arranged by size:
4955 907 784 500 218
PasI CC'CwG_GG
Cuts at: 2612 3997 2612
Size: 1385 5979
PciI A'CATG_T
Cuts at: 3411 4195 5820 3411
Size: 784 1625 4955
Fragments arranged by size:
4955 1625 784
PmeI GTTT'AAAC
Cuts at: 4363 4363
Size: 7364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6503 6535 6503
Size: 32 7332
PpuMI rG'GwC_Cy
Cuts at: 1077 3418 1077
Size: 2341 5023
PsiI TTA'TAA
Cuts at: 3121 3323 3121
Size: 202 7162
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3353 3385 3353
Size: 32 7332
PstI C_TGCA'G
Cuts at: 4411 5096 4411
Size: 685 6679
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7364
PvuII CAG'CTG
Cuts at: 1852 2640 3982 4789 5149 1852
Size: 788 1342 807 360 4067
Fragments arranged by size:
4067 1342 807 788 360
RsrII CG'GwC_CG
Cuts at: 5559 5559
Size: 7364
SacI G_AGCT'C
Cuts at: 4052 4378 4052
Size: 326 7038
SalI G'TCGA_C
Cuts at: 3451 4401 3451
Size: 950 6414
SapI GCTCTTCn'nnn_
Cuts at: 5387 5597 5387
Size: 210 7154
SbfI CC_TGCA'GG
Cuts at: 4411 4411
Size: 7364
ScaI AGT'ACT
Cuts at: 6881 6881
Size: 7364
SfcI C'TryA_G
Cuts at: 33 133 1442 4407 5092 6085 6276 33
Size: 100 1309 2965 685 993 191 1121
Fragments arranged by size:
2965 1309 1121 993 685 191 100
SfoI GGC'GCC
Cuts at: 4796 5044 4796
Size: 248 7116
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7364
SmaI CCC'GGG
Cuts at: 502 4386 502
Size: 3884 3480
SmlI C'TyrA_G
Cuts at: 514 2253 3844 4265 4336 4519 5434 5926 6188
Size: 1739 1591 421 71 183 915 492 262
Cuts at: 6188 514
Size: 1690
Fragments arranged by size:
1739 1690 1591 915 492 421 262 183
71
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7364
SphI G_CATG'C
Cuts at: 4417 4917 4417
Size: 500 6864
SspI AAT'ATT
Cuts at: 2352 3825 2352
Size: 1473 5891
StuI AGG'CCT
Cuts at: 1401 1575 1401
Size: 174 7190
StyI C'CwwG_G
Cuts at: 107 1527 2294 2816 4172 4498 4852 107
Size: 1420 767 522 1356 326 354 2619
Fragments arranged by size:
2619 1420 1356 767 522 354 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2780 3296 4947 5080 5539 5748 2780
Size: 516 1651 133 459 209 4396
Fragments arranged by size:
4396 1651 516 459 209 133
TatI w'GTAC_w
Cuts at: 3645 6879 3645
Size: 3234 4130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2592 2624 3459 3491 6881 6913 2592
Size: 32 835 32 3390 32 3043
Fragments arranged by size:
3390 3043 835 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5161 5161
Size: 7364
XbaI T'CTAG_A
Cuts at: 4395 4395
Size: 7364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4290 4859 4290
Size: 569 6795
XmaI C'CCGG_G
Cuts at: 500 4384 500
Size: 3884 3480
XmnI GAAnn'nnTTC
Cuts at: 1307 1307
Size: 7364
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PmeI PpiI PpuMI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AloI ApaI AscI BaeI BcgI
BglI BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
CspCI FseI FspAI HpaI NheI NotI NruI PacI
PflMI PfoI PmlI PshAI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI