Restriction map for
pF1KE9668
(Circular) (Six-base) MAPSORT of: pF1KE9668.seq Check: 4608 from: 1 to: 7199
pF1KE9668 7199 bp
With 182 enzymes: SgfI *
December 21, 2016 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2603 2630 2871 3551 2603
Size: 27 241 680 6251
Fragments arranged by size:
6251 680 241 27
AatII G_ACGT'C
Cuts at: 1373 1373
Size: 7199
AccI GT'mk_AC
Cuts at: 187 4237 187
Size: 4050 3149
Acc65I G'GTAC_C
Cuts at: 693 4215 693
Size: 3522 3677
AclI AA'CG_TT
Cuts at: 7039 7039
Size: 7199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2059 2104 3465 3850 5024 5456 6203 6471 2059
Size: 45 1361 385 1174 432 747 268 2787
Fragments arranged by size:
2787 1361 1174 747 432 385 268 45
AfeI AGC'GCT
Cuts at: 445 2113 445
Size: 1668 5531
AflIII A'CryG_T
Cuts at: 4551 5655 6632 4551
Size: 1104 977 5118
Fragments arranged by size:
5118 1104 977
AgeI A'CCGG_T
Cuts at: 3274 5587 3274
Size: 2313 4886
AleI CACnn'nnGTG
Cuts at: 2518 2518
Size: 7199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4028 4028
Size: 7199
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3691 3723 3691
Size: 32 7167
AlwNI CAG_nnn'CTG
Cuts at: 846 1638 2910 3015 3342 3393 4504 6071 846
Size: 792 1272 105 327 51 1111 1567 1974
Fragments arranged by size:
1974 1567 1272 1111 792 327 105 51
ApaI G_GGCC'C
Cuts at: 3630 3933 3630
Size: 303 6896
ApaLI G'TGCA_C
Cuts at: 1669 2008 3097 5969 1669
Size: 339 1089 2872 2899
Fragments arranged by size:
2899 2872 1089 339
ApoI r'AATT_y
Cuts at: 2043 4149 4203 6552 6808 2043
Size: 2106 54 2349 256 2434
Fragments arranged by size:
2434 2349 2106 256 54
AseI AT'TA_AT
Cuts at: 20 20
Size: 7199
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7199
AvaI C'yCGr_G
Cuts at: 2299 3800 4219 2299
Size: 1501 419 5279
Fragments arranged by size:
5279 1501 419
BamHI G'GATC_C
Cuts at: 4224 4224
Size: 7199
BanI G'GyrC_C
Cuts at: 693 831 2294 2520 3248 3950 4215 4629 4877
Size: 138 1463 226 728 702 265 414 248
Cuts at: 4877 4912 693
Size: 35 2980
Fragments arranged by size:
2980 1463 728 702 414 265 248 226
138 35
BanII G_rGCy'C
Cuts at: 1046 1632 1649 2033 2224 2537 3479 3506 3630
Size: 586 17 384 191 313 942 27 124
Cuts at: 3630 3709 3933 4051 4213 5243 1046
Size: 79 224 118 162 1030 3002
Fragments arranged by size:
3002 1030 942 586 384 313 224 191
162 124 118 79 27 17
BbeI G_GCGC'C
Cuts at: 3954 4633 4881 3954
Size: 679 248 6272
Fragments arranged by size:
6272 679 248
BbsI GAAGACnn'nnnn_
Cuts at: 961 2258 3667 4119 961
Size: 1297 1409 452 4041
Fragments arranged by size:
4041 1409 1297 452
BbvCI CC'TCA_GC
Cuts at: 740 3215 740
Size: 2475 4724
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1663 1697 1663
Size: 34 7165
BciVI GTATCCnnnnn_n'
Cuts at: 493 5097 5858 7199 493
Size: 4604 761 1341 493
Fragments arranged by size:
4604 1341 761 493
BclI T'GATC_A
Cuts at: 4719 4719
Size: 7199
BglI GCCn_nnn'nGGC
Cuts at: 279 2721 2763 3552 3832 3928 279
Size: 2442 42 789 280 96 3550
Fragments arranged by size:
3550 2442 789 280 96 42
BglII A'GATC_T
Cuts at: 4714 6751 4714
Size: 2037 5162
BlpI GC'TnA_GC
Cuts at: 1643 1960 4311 1643
Size: 317 2351 4531
Fragments arranged by size:
4531 2351 317
Bme1580I G_kGCm'C
Cuts at: 869 1115 1529 1673 2012 2297 2369 2525 3101
Size: 246 414 144 339 285 72 156 576
Cuts at: 3101 3630 3933 4824 4917 5973 869
Size: 529 303 891 93 1056 2095
Fragments arranged by size:
2095 1056 891 576 529 414 339 303
285 246 156 144 93 72
BmgBI CAC'GTC
Cuts at: 649 1110 1251 3946 649
Size: 461 141 2695 3902
Fragments arranged by size:
3902 2695 461 141
BmrI ACTGGGnnnn_n'
Cuts at: 1270 4053 4411 4492 4827 6971 1270
Size: 2783 358 81 335 2144 1498
Fragments arranged by size:
2783 2144 1498 358 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 334 1044 2253 5566 334
Size: 710 1209 3313 1967
Fragments arranged by size:
3313 1967 1209 710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 878 1348 1429 1633 2128 2208 2551 2669
Size: 324 470 81 204 495 80 343 118
Cuts at: 2669 2866 3650 3934 554
Size: 197 784 284 3819
Fragments arranged by size:
3819 784 495 470 343 324 284 204
197 118 81 80
Bpu10I CC'TnA_GC
Cuts at: 473 740 1352 2606 2636 3215 3836 473
Size: 267 612 1254 30 579 621 3836
Fragments arranged by size:
3836 1254 621 612 579 267 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 692 1153 1174 2135 4375 5254 5746 6044
Size: 388 461 21 961 2240 879 492 298
Cuts at: 6044 304
Size: 1459
Fragments arranged by size:
2240 1459 961 879 492 461 388 298
21
BsaI GGTCTCn'nnnn_
Cuts at: 247 1161 2071 2682 6903 247
Size: 914 910 611 4221 543
Fragments arranged by size:
4221 914 910 611 543
BsaAI yAC'GTr
Cuts at: 1901 4552 1901
Size: 2651 4548
BsaBI GATnn'nnATC
Cuts at: 6 2065 4713 6750 6
Size: 2059 2648 2037 455
Fragments arranged by size:
2648 2059 2037 455
BsaHI Gr'CG_yC
Cuts at: 853 1370 3951 4116 4630 4878 5580 7076 853
Size: 517 2581 165 514 248 702 1496 976
Fragments arranged by size:
2581 1496 976 702 517 514 248 165
BsaWI w'CCGG_w
Cuts at: 3274 4388 4909 5587 5861 6008 6419 3274
Size: 1114 521 678 274 147 411 4054
Fragments arranged by size:
4054 1114 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2789 2819 3691 3721 2789
Size: 30 872 30 6267
Fragments arranged by size:
6267 872 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 710 743 1507 1575 1633 1636 1802 1960 2172
Size: 33 764 68 58 3 166 158 212
Cuts at: 2172 2229 2672 3457 3738 3826 3903 3952 710
Size: 57 443 785 281 88 77 49 3957
Fragments arranged by size:
3957 785 764 443 281 212 166 158
88 77 68 58 57 49 33 3
BseYI C'CCAG_C
Cuts at: 212 839 872 902 1059 1131 1631 1700 1995
Size: 627 33 30 157 72 500 69 295
Cuts at: 1995 2369 2390 2537 2579 2627 2708 2725 2769
Size: 374 21 147 42 48 81 17 44
Cuts at: 2769 2963 3047 3053 3065 3362 3419 3548 4052
Size: 194 84 6 12 297 57 129 504
Cuts at: 4052 4624 5382 5959 6455 212
Size: 572 758 577 496 956
Fragments arranged by size:
956 758 627 577 572 504 500 496
374 297 295 194 157 147 129 84
81 72 69 57 48 44 42 33
30 21 17 12 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 2575 2941 2977 3025 3745 781
Size: 1794 366 36 48 720 4235
Fragments arranged by size:
4235 1794 720 366 48 36
BsiEI CG_ry'CG
Cuts at: 104 3499 3556 4741 4787 5995 104
Size: 3395 57 1185 46 1208 1308
Fragments arranged by size:
3395 1308 1208 1185 57 46
BsiHKAI G_wGCw'C
Cuts at: 320 1312 1649 1673 2012 2224 2642 3101 3848
Size: 992 337 24 339 212 418 459 747
Cuts at: 3848 4051 4213 4991 5181 5973 320
Size: 203 162 778 190 792 1546
Fragments arranged by size:
1546 992 792 778 747 459 418 339
337 212 203 190 162 24
BsmI GAATG_Cn'
Cuts at: 474 474
Size: 7199
Bsp1286I G_dGCh'C
Cuts at: 320 869 1046 1115 1312 1529 1632 1649 1673
Size: 549 177 69 197 217 103 17 24
Cuts at: 1673 2012 2033 2224 2297 2369 2525 2537 2642
Size: 339 21 191 73 72 156 12 105
Cuts at: 2642 3101 3479 3506 3630 3709 3848 3933 4051
Size: 459 378 27 124 79 139 85 118
Cuts at: 4051 4213 4824 4917 4991 5181 5243 5973 320
Size: 162 611 93 74 190 62 730 1546
Fragments arranged by size:
1546 730 611 549 459 378 339 217
197 191 190 177 162 156 139 124
118 105 103 93 85 79 74 73
72 69 62 27 24 21 17 12
BspEI T'CCGG_A
Cuts at: 6419 6419
Size: 7199
BspHI T'CATG_A
Cuts at: 620 821 6375 620
Size: 201 5554 1444
Fragments arranged by size:
5554 1444 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 311 2603 2630 2871 2909 3551 4249 4463 4765
Size: 2292 27 241 38 642 698 214 302
Cuts at: 4765 5146 311
Size: 381 2364
Fragments arranged by size:
2364 2292 698 642 381 302 241 214
38 27
BsrBI CCG'CTC
Cuts at: 50 122 892 3799 3867 5491 5545 7029 7194
Size: 72 770 2907 68 1624 54 1484 165
Cuts at: 7194 50
Size: 55
Fragments arranged by size:
2907 1624 1484 770 165 72 68 55
54
BsrDI GCAATG_nn'
Cuts at: 1857 2507 5111 1857
Size: 650 2604 3945
Fragments arranged by size:
3945 2604 650
BsrFI r'CCGG_y
Cuts at: 1082 2176 3274 5197 5378 5587 1082
Size: 1094 1098 1923 181 209 2694
Fragments arranged by size:
2694 1923 1098 1094 209 181
BssSI C'ACGA_G
Cuts at: 1018 1672 3427 5470 5828 1018
Size: 654 1755 2043 358 2389
Fragments arranged by size:
2389 2043 1755 654 358
BstAPI GCAn_nnn'nTGC
Cuts at: 303 2910 2919 3015 3111 4576 303
Size: 2607 9 96 96 1465 2926
Fragments arranged by size:
2926 2607 1465 96 96 9
BstBI TT'CG_AA
Cuts at: 1457 5560 1457
Size: 4103 3096
BstEII G'GTnAC_C
Cuts at: 3277 3277
Size: 7199
BstXI CCAn_nnnn'nTGG
Cuts at: 1059 1545 1059
Size: 486 6713
BstYI r'GATC_y
Cuts at: 1 3449 4224 4691 4714 5049 5295 6307 6393
Size: 3448 775 467 23 335 246 1012 86
Cuts at: 6393 6405 6751 1
Size: 12 346 449
Fragments arranged by size:
3448 1012 775 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1490 1490
Size: 7199
BtgI C'CryG_G
Cuts at: 689 1298 1430 2819 3088 3407 4127 689
Size: 609 132 1389 269 319 720 3761
Fragments arranged by size:
3761 1389 720 609 319 269 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 915 2497 5149 5330 5493 915
Size: 1582 2652 181 163 2621
Fragments arranged by size:
2652 2621 1582 181 163
BtsI GCAGTG_nn'
Cuts at: 844 1529 2095 2678 4442 844
Size: 685 566 583 1764 3601
Fragments arranged by size:
3601 1764 685 583 566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 987 1022 1524 1559 987
Size: 35 502 35 6627
Fragments arranged by size:
6627 502 35 35
DraI TTT'AAA
Cuts at: 4198 4198
Size: 7199
DraIII CAC_nnn'GTG
Cuts at: 2101 2673 2101
Size: 572 6627
DrdI GACnn_nn'nnGTC
Cuts at: 805 3351 4905 5763 805
Size: 2546 1554 858 2241
Fragments arranged by size:
2546 2241 1554 858
EaeI y'GGCC_r
Cuts at: 935 1427 1505 2568 3496 3553 4784 4958 5349
Size: 492 78 1063 928 57 1231 174 391
Cuts at: 5349 5376 6721 935
Size: 27 1345 1413
Fragments arranged by size:
1413 1345 1231 1063 928 492 391 174
78 57 27
EagI C'GGCC_G
Cuts at: 3496 3553 4784 3496
Size: 57 1231 5911
Fragments arranged by size:
5911 1231 57
EarI CTCTTCn'nnn_
Cuts at: 580 636 967 1138 1775 1876 1910 2849 5222
Size: 56 331 171 637 101 34 939 2373
Cuts at: 5222 5432 580
Size: 210 2347
Fragments arranged by size:
2373 2347 939 637 331 210 171 101
56 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3161 3778 5407 5717 5863 7009 3161
Size: 617 1629 310 146 1146 3351
Fragments arranged by size:
3351 1629 1146 617 310 146
EcoICRI GAG'CTC
Cuts at: 1647 2222 4049 4211 1647
Size: 575 1827 162 4635
Fragments arranged by size:
4635 1827 575 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 878 1348 1429 1633 2059 2104 2128 2208
Size: 324 470 81 204 426 45 24 80
Cuts at: 2208 2551 2669 2866 3465 3650 3850 3934 5024
Size: 343 118 197 599 185 200 84 1090
Cuts at: 5024 5456 6203 6471 554
Size: 432 747 268 1282
Fragments arranged by size:
1282 1090 747 599 470 432 426 343
324 268 204 200 197 185 118 84
81 80 45 24
EcoO109I rG'GnC_Cy
Cuts at: 2352 3531 3626 3627 3684 3825 4338 2352
Size: 1179 95 1 57 141 513 5213
Fragments arranged by size:
5213 1179 513 141 95 57 1
EcoRI G'AATT_C
Cuts at: 2043 4203 2043
Size: 2160 5039
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 462 594 4204 5218 462
Size: 132 3610 1014 2443
Fragments arranged by size:
3610 2443 1014 132
FspI TGC'GCA
Cuts at: 4980 4980
Size: 7199
HaeII r_GCGC'y
Cuts at: 447 1876 2115 3954 3978 4633 4881 5903 6882
Size: 1429 239 1839 24 655 248 1022 979
Cuts at: 6882 447
Size: 764
Fragments arranged by size:
1839 1429 1022 979 764 655 248 239
24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1570 1602 3253 3285 4153 4185 5041 5071 5073
Size: 32 1651 32 868 32 856 30 2
Cuts at: 5073 5103 1570
Size: 30 3666
Fragments arranged by size:
3666 1651 868 856 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 4238 4238
Size: 7199
KasI G'GCGC_C
Cuts at: 3950 4629 4877 3950
Size: 679 248 6272
Fragments arranged by size:
6272 679 248
KpnI G_GTAC'C
Cuts at: 697 4219 697
Size: 3522 3677
MluI A'CGCG_T
Cuts at: 6632 6632
Size: 7199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 284 1412 1889 2072 2195 3156 3239 3760 5870
Size: 1128 477 183 123 961 83 521 2110
Cuts at: 5870 6054 284
Size: 184 1429
Fragments arranged by size:
2110 1429 1128 961 521 477 184 183
123 83
MscI TGG'CCA
Cuts at: 1429 4960 1429
Size: 3531 3668
MslI CAynn'nnrTG
Cuts at: 826 1303 2518 2881 2887 4144 5188 5315 826
Size: 477 1215 363 6 1257 1044 127 2710
Fragments arranged by size:
2710 1257 1215 1044 477 363 127 6
MspA1I CmG'CkG
Cuts at: 1068 1181 1635 2740 2895 2910 3015 3552 4012
Size: 113 454 1105 155 15 105 537 460
Cuts at: 4012 4310 4624 4984 5997 6242 1068
Size: 298 314 360 1013 245 2025
Fragments arranged by size:
2025 1105 1013 537 460 454 360 314
298 245 155 113 105 15
NaeI GCC'GGC
Cuts at: 1084 2178 5380 1084
Size: 1094 3202 2903
Fragments arranged by size:
3202 2903 1094
NarI GG'CG_CC
Cuts at: 3951 4630 4878 3951
Size: 679 248 6272
Fragments arranged by size:
6272 679 248
NcoI C'CATG_G
Cuts at: 1298 1430 2819 4127 1298
Size: 132 1389 1308 4370
Fragments arranged by size:
4370 1389 1308 132
NgoMIV G'CCGG_C
Cuts at: 1082 2176 5378 1082
Size: 1094 3202 2903
Fragments arranged by size:
3202 2903 1094
NspI r_CATG'y
Cuts at: 4252 4752 5659 4252
Size: 500 907 5792
Fragments arranged by size:
5792 907 500
PasI CC'CwG_GG
Cuts at: 127 3150 127
Size: 3023 4176
PciI A'CATG_T
Cuts at: 5655 5655
Size: 7199
PflMI CCAn_nnn'nTGG
Cuts at: 1422 1638 1422
Size: 216 6983
PfoI T'CCnGG_A
Cuts at: 485 1445 1649 2528 485
Size: 960 204 879 5156
Fragments arranged by size:
5156 960 879 204
PmeI GTTT'AAAC
Cuts at: 4198 4198
Size: 7199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3691 3723 6338 6370 3691
Size: 32 2615 32 4520
Fragments arranged by size:
4520 2615 32 32
PpuMI rG'GwC_Cy
Cuts at: 3684 3684
Size: 7199
PshAI GACnn'nnGTC
Cuts at: 817 817
Size: 7199
PspOMI G'GGCC_C
Cuts at: 3626 3929 3626
Size: 303 6896
PstI C_TGCA'G
Cuts at: 851 1180 2906 2915 3020 4246 4931 851
Size: 329 1726 9 105 1226 685 3119
Fragments arranged by size:
3119 1726 1226 685 329 105 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7199
PvuII CAG'CTG
Cuts at: 2895 2910 3015 4012 4624 4984 2895
Size: 15 105 997 612 360 5110
Fragments arranged by size:
5110 997 612 360 105 15
RsrII CG'GwC_CG
Cuts at: 5394 5394
Size: 7199
SacI G_AGCT'C
Cuts at: 1649 2224 4051 4213 1649
Size: 575 1827 162 4635
Fragments arranged by size:
4635 1827 575 162
SalI G'TCGA_C
Cuts at: 4236 4236
Size: 7199
SapI GCTCTTCn'nnn_
Cuts at: 580 1138 1876 2849 5222 5432 580
Size: 558 738 973 2373 210 2347
Fragments arranged by size:
2373 2347 973 738 558 210
SbfI CC_TGCA'GG
Cuts at: 4246 4246
Size: 7199
ScaI AGT'ACT
Cuts at: 6716 6716
Size: 7199
SfcI C'TryA_G
Cuts at: 33 847 1176 2902 2911 3016 4242 4927 5920
Size: 814 329 1726 9 105 1226 685 993
Cuts at: 5920 6111 33
Size: 191 1121
Fragments arranged by size:
1726 1226 1121 993 814 685 329 191
105 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3928 3928
Size: 7199
SfoI GGC'GCC
Cuts at: 3952 4631 4879 3952
Size: 679 248 6272
Fragments arranged by size:
6272 679 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7199
SmaI CCC'GGG
Cuts at: 2301 4221 2301
Size: 1920 5279
SmlI C'TyrA_G
Cuts at: 319 707 1168 1189 2150 4354 5269 5761 6023
Size: 388 461 21 961 2204 915 492 262
Cuts at: 6023 319
Size: 1495
Fragments arranged by size:
2204 1495 961 915 492 461 388 262
21
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7199
SphI G_CATG'C
Cuts at: 4252 4752 4252
Size: 500 6699
SspI AAT'ATT
Cuts at: 4175 4175
Size: 7199
StuI AGG'CCT
Cuts at: 3585 3585
Size: 7199
StyI C'CwwG_G
Cuts at: 1298 1430 1538 1802 2819 4127 4333 4687 1298
Size: 132 108 264 1017 1308 206 354 3810
Fragments arranged by size:
3810 1308 1017 354 264 206 132 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 935 939 1145 2530 2593 3139 3474 4782 4915
Size: 4 206 1385 63 546 335 1308 133
Cuts at: 4915 5374 5583 935
Size: 459 209 2551
Fragments arranged by size:
2551 1385 1308 546 459 335 209 206
133 63 4
TatI w'GTAC_w
Cuts at: 1400 6714 1400
Size: 5314 1885
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2789 2821 3449 3481 6716 6748 2789
Size: 32 628 32 3235 32 3240
Fragments arranged by size:
3240 3235 628 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 544 4996 544
Size: 4452 2747
XbaI T'CTAG_A
Cuts at: 4230 4230
Size: 7199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1542 4694 1542
Size: 3152 4047
XmaI C'CCGG_G
Cuts at: 2299 4219 2299
Size: 1920 5279
XmnI GAAnn'nnTTC
Cuts at: 2197 2197
Size: 7199
ZraI GAC'GTC
Cuts at: 1371 1371
Size: 7199
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AscI AvrII BaeI BfrBI BmtI BsiWI
BsmBI BsrGI BssHII BstZ17I ClaI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PsiI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SwaI XhoI