Restriction map for
pF1KE9656
(Circular) (Six-base) MAPSORT of: pF1KE9656.seq Check: 5408 from: 1 to: 3605
pF1KE9656 3605 bp
With 182 enzymes: SgfI *
March 10, 2017 10:38 ..
AccI GT'mk_AC
Cuts at: 309 643 309
Size: 334 3271
Acc65I G'GTAC_C
Cuts at: 621 621
Size: 3605
AclI AA'CG_TT
Cuts at: 3445 3445
Size: 3605
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 1430 1862 2609 2877 468
Size: 962 432 747 268 1196
Fragments arranged by size:
1196 962 747 432 268
AflIII A'CryG_T
Cuts at: 957 2061 3038 957
Size: 1104 977 1524
Fragments arranged by size:
1524 1104 977
AgeI A'CCGG_T
Cuts at: 1993 1993
Size: 3605
AlwNI CAG_nnn'CTG
Cuts at: 910 2477 910
Size: 1567 2038
ApaI G_GGCC'C
Cuts at: 230 274 230
Size: 44 3561
ApaLI G'TGCA_C
Cuts at: 2375 2375
Size: 3605
ApoI r'AATT_y
Cuts at: 609 2958 3214 609
Size: 2349 256 1000
Fragments arranged by size:
2349 1000 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3605
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3605
AvaI C'yCGr_G
Cuts at: 274 592 625 274
Size: 318 33 3254
Fragments arranged by size:
3254 318 33
BamHI G'GATC_C
Cuts at: 630 630
Size: 3605
BanI G'GyrC_C
Cuts at: 140 330 588 621 1035 1283 1318 140
Size: 190 258 33 414 248 35 2427
Fragments arranged by size:
2427 414 258 248 190 35 33
BanII G_rGCy'C
Cuts at: 230 274 619 1649 230
Size: 44 345 1030 2186
Fragments arranged by size:
2186 1030 345 44
BbeI G_GCGC'C
Cuts at: 1039 1287 1039
Size: 248 3357
BciVI GTATCCnnnnn_n'
Cuts at: 343 1503 2264 3605 343
Size: 1160 761 1341 343
Fragments arranged by size:
1341 1160 761 343
BclI T'GATC_A
Cuts at: 1125 1125
Size: 3605
BglII A'GATC_T
Cuts at: 1120 3157 1120
Size: 2037 1568
BlpI GC'TnA_GC
Cuts at: 717 717
Size: 3605
Bme1580I G_kGCm'C
Cuts at: 230 274 593 1230 1323 2379 230
Size: 44 319 637 93 1056 1456
Fragments arranged by size:
1456 1056 637 319 93 44
BmrI ACTGGGnnnn_n'
Cuts at: 817 898 1233 3377 817
Size: 81 335 2144 1045
Fragments arranged by size:
2144 1045 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1972 1972
Size: 3605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 529 463
Size: 66 3539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 1660 2152 2450 781
Size: 879 492 298 1936
Fragments arranged by size:
1936 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 505 3309 505
Size: 2804 801
BsaAI yAC'GTr
Cuts at: 354 958 354
Size: 604 3001
BsaBI GATnn'nnATC
Cuts at: 6 1119 3156 6
Size: 1113 2037 455
Fragments arranged by size:
2037 1113 455
BsaHI Gr'CG_yC
Cuts at: 176 1036 1284 1986 3482 176
Size: 860 248 702 1496 299
Fragments arranged by size:
1496 860 702 299 248
BsaWI w'CCGG_w
Cuts at: 794 1315 1993 2267 2414 2825 794
Size: 521 678 274 147 411 1574
Fragments arranged by size:
1574 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 142 172 308 338 142
Size: 30 136 30 3409
Fragments arranged by size:
3409 136 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 334 445 448 334
Size: 111 3 3491
Fragments arranged by size:
3491 111 3
BseYI C'CCAG_C
Cuts at: 559 1030 1788 2365 2861 559
Size: 471 758 577 496 1303
Fragments arranged by size:
1303 758 577 496 471
BsiEI CG_ry'CG
Cuts at: 104 459 1147 1193 2401 104
Size: 355 688 46 1208 1308
Fragments arranged by size:
1308 1208 688 355 46
BsiHKAI G_wGCw'C
Cuts at: 619 1397 1587 2379 619
Size: 778 190 792 1845
Fragments arranged by size:
1845 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 230 274 593 619 1230 1323 1397 1587 1649
Size: 44 319 26 611 93 74 190 62
Cuts at: 1649 2379 230
Size: 730 1456
Fragments arranged by size:
1456 730 611 319 190 93 74 62
44 26
BspEI T'CCGG_A
Cuts at: 2825 2825
Size: 3605
BspHI T'CATG_A
Cuts at: 2781 2781
Size: 3605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 655 869 1171 1552 655
Size: 214 302 381 2708
Fragments arranged by size:
2708 381 302 214
BsrBI CCG'CTC
Cuts at: 50 116 447 1897 1951 3435 3600 50
Size: 66 331 1450 54 1484 165 55
Fragments arranged by size:
1484 1450 331 165 66 55 54
BsrDI GCAATG_nn'
Cuts at: 166 1517 166
Size: 1351 2254
BsrFI r'CCGG_y
Cuts at: 1603 1784 1993 1603
Size: 181 209 3215
Fragments arranged by size:
3215 209 181
BssSI C'ACGA_G
Cuts at: 343 1876 2234 343
Size: 1533 358 1714
Fragments arranged by size:
1714 1533 358
BstAPI GCAn_nnn'nTGC
Cuts at: 201 982 201
Size: 781 2824
BstBI TT'CG_AA
Cuts at: 1966 1966
Size: 3605
BstYI r'GATC_y
Cuts at: 1 630 1097 1120 1455 1701 2713 2799 2811
Size: 629 467 23 335 246 1012 86 12
Cuts at: 2811 3157 1
Size: 346 449
Fragments arranged by size:
1012 629 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3605
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1555 1736 1899 1555
Size: 181 163 3261
Fragments arranged by size:
3261 181 163
BtsI GCAGTG_nn'
Cuts at: 190 473 848 190
Size: 283 375 2947
Fragments arranged by size:
2947 375 283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 522 557 522
Size: 35 3570
DraI TTT'AAA
Cuts at: 604 604
Size: 3605
DraIII CAC_nnn'GTG
Cuts at: 500 500
Size: 3605
DrdI GACnn_nn'nnGTC
Cuts at: 1311 2169 1311
Size: 858 2747
EaeI y'GGCC_r
Cuts at: 128 1190 1364 1755 1782 3127 128
Size: 1062 174 391 27 1345 606
Fragments arranged by size:
1345 1062 606 391 174 27
EagI C'GGCC_G
Cuts at: 1190 1190
Size: 3605
EarI CTCTTCn'nnn_
Cuts at: 362 1628 1838 362
Size: 1266 210 2129
Fragments arranged by size:
2129 1266 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 150 253 1813 2123 2269 3415 150
Size: 103 1560 310 146 1146 340
Fragments arranged by size:
1560 1146 340 310 146 103
EcoICRI GAG'CTC
Cuts at: 617 617
Size: 3605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 468 529 1430 1862 2609 2877 463
Size: 5 61 901 432 747 268 1191
Fragments arranged by size:
1191 901 747 432 268 61 5
EcoO109I rG'GnC_Cy
Cuts at: 270 271 545 744 270
Size: 1 274 199 3131
Fragments arranged by size:
3131 274 199 1
EcoRI G'AATT_C
Cuts at: 609 609
Size: 3605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1624 1624
Size: 3605
FspI TGC'GCA
Cuts at: 1386 1386
Size: 3605
HaeII r_GCGC'y
Cuts at: 246 1039 1287 2309 3288 246
Size: 793 248 1022 979 563
Fragments arranged by size:
1022 979 793 563 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1447 1477 1479 1509 1447
Size: 30 2 30 3543
Fragments arranged by size:
3543 30 30 2
HincII GTy'rAC
Cuts at: 644 644
Size: 3605
KasI G'GCGC_C
Cuts at: 1035 1283 1035
Size: 248 3357
KpnI G_GTAC'C
Cuts at: 625 625
Size: 3605
MluI A'CGCG_T
Cuts at: 3038 3038
Size: 3605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2276 2460 2276
Size: 184 3421
MscI TGG'CCA
Cuts at: 1366 1366
Size: 3605
MslI CAynn'nnrTG
Cuts at: 1594 1721 1594
Size: 127 3478
MspA1I CmG'CkG
Cuts at: 305 441 716 1030 1390 2403 2648 305
Size: 136 275 314 360 1013 245 1262
Fragments arranged by size:
1262 1013 360 314 275 245 136
NaeI GCC'GGC
Cuts at: 1786 1786
Size: 3605
NarI GG'CG_CC
Cuts at: 1036 1284 1036
Size: 248 3357
NcoI C'CATG_G
Cuts at: 107 107
Size: 3605
NgoMIV G'CCGG_C
Cuts at: 1784 1784
Size: 3605
NspI r_CATG'y
Cuts at: 658 1158 2065 658
Size: 500 907 2198
Fragments arranged by size:
2198 907 500
PciI A'CATG_T
Cuts at: 2061 2061
Size: 3605
PfoI T'CCnGG_A
Cuts at: 412 412
Size: 3605
PmeI GTTT'AAAC
Cuts at: 604 604
Size: 3605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2744 2776 2744
Size: 32 3573
PspOMI G'GGCC_C
Cuts at: 226 270 226
Size: 44 3561
PstI C_TGCA'G
Cuts at: 652 1337 652
Size: 685 2920
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3605
PvuII CAG'CTG
Cuts at: 1030 1390 1030
Size: 360 3245
RsrII CG'GwC_CG
Cuts at: 1800 1800
Size: 3605
SacI G_AGCT'C
Cuts at: 619 619
Size: 3605
SalI G'TCGA_C
Cuts at: 642 642
Size: 3605
SapI GCTCTTCn'nnn_
Cuts at: 1628 1838 1628
Size: 210 3395
SbfI CC_TGCA'GG
Cuts at: 652 652
Size: 3605
ScaI AGT'ACT
Cuts at: 3122 3122
Size: 3605
SfcI C'TryA_G
Cuts at: 33 648 1333 2326 2517 33
Size: 615 685 993 191 1121
Fragments arranged by size:
1121 993 685 615 191
SfoI GGC'GCC
Cuts at: 1037 1285 1037
Size: 248 3357
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3605
SmaI CCC'GGG
Cuts at: 627 627
Size: 3605
SmlI C'TyrA_G
Cuts at: 760 1675 2167 2429 760
Size: 915 492 262 1936
Fragments arranged by size:
1936 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3605
SphI G_CATG'C
Cuts at: 658 1158 658
Size: 500 3105
StyI C'CwwG_G
Cuts at: 107 739 1093 107
Size: 632 354 2619
Fragments arranged by size:
2619 632 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1188 1321 1780 1989 1188
Size: 133 459 209 2804
Fragments arranged by size:
2804 459 209 133
TatI w'GTAC_w
Cuts at: 3120 3120
Size: 3605
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 142 174 272 304 308 340 3122 3154 142
Size: 32 98 32 4 32 2782 32 593
Fragments arranged by size:
2782 593 98 32 32 32 32 4
Tth111I GACn'n_nGTC
Cuts at: 1402 1402
Size: 3605
XbaI T'CTAG_A
Cuts at: 636 636
Size: 3605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1100 1100
Size: 3605
XmaI C'CCGG_G
Cuts at: 625 625
Size: 3605
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI