Restriction map for
pF1KE9645
(Circular) (Six-base) MAPSORT of: pF1KE9645.seq Check: 9397 from: 1 to: 10589
pF1KE9645 10589 bp
With 182 enzymes: SgfI *
May 29, 2012 11:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5701 6678 5701
Size: 977 9612
AccI GT'mk_AC
Cuts at: 7233 7627 7233
Size: 394 10195
Acc65I G'GTAC_C
Cuts at: 7605 7605
Size: 10589
AclI AA'CG_TT
Cuts at: 10429 10429
Size: 10589
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 293 311 1658 2339 2678 2759 3062 3077
Size: 78 18 1347 681 339 81 303 15
Cuts at: 3077 3095 3320 3614 3824 3965 4112 6425 6814
Size: 18 225 294 210 141 147 2313 389
Cuts at: 6814 6929 8414 8846 9593 9861 215
Size: 115 1485 432 747 268 943
Fragments arranged by size:
2313 1485 1347 943 747 681 432 389
339 303 294 268 225 210 147 141
115 81 78 18 18 15
AflII C'TTAA_G
Cuts at: 1282 4597 1282
Size: 3315 7274
AflIII A'CryG_T
Cuts at: 2079 2965 7941 9045 10022 2079
Size: 886 4976 1104 977 2646
Fragments arranged by size:
4976 2646 1104 977 886
AgeI A'CCGG_T
Cuts at: 1573 8977 1573
Size: 7404 3185
AhdI GACnn_n'nnGTC
Cuts at: 637 637
Size: 10589
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2867 2867
Size: 10589
AlwNI CAG_nnn'CTG
Cuts at: 2278 2299 2362 3871 4090 5245 5851 6133 6660
Size: 21 63 1509 219 1155 606 282 527
Cuts at: 6660 7498 7894 9461 2278
Size: 838 396 1567 3406
Fragments arranged by size:
3406 1567 1509 1155 838 606 527 396
282 219 63 21
ApaLI G'TGCA_C
Cuts at: 7529 9359 7529
Size: 1830 8759
ApoI r'AATT_y
Cuts at: 438 541 963 1222 1347 2289 2375 2627 2706
Size: 103 422 259 125 942 86 252 79
Cuts at: 2706 3007 3179 3522 3747 3801 4544 5017 5313
Size: 301 172 343 225 54 743 473 296
Cuts at: 5313 5409 5581 5833 5982 6182 6409 7434 7593
Size: 96 172 252 149 200 227 1025 159
Cuts at: 7593 9942 10198 438
Size: 2349 256 829
Fragments arranged by size:
2349 1025 942 829 743 473 422 343
301 296 259 256 252 252 227 225
200 172 172 159 149 125 103 96
86 79 54
AseI AT'TA_AT
Cuts at: 20 2369 6479 7091 20
Size: 2349 4110 612 3518
Fragments arranged by size:
4110 3518 2349 612
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10589
AvaI C'yCGr_G
Cuts at: 5993 7609 5993
Size: 1616 8973
BamHI G'GATC_C
Cuts at: 5419 7614 5419
Size: 2195 8394
BanI G'GyrC_C
Cuts at: 7605 8019 8267 8302 7605
Size: 414 248 35 9892
Fragments arranged by size:
9892 414 248 35
BanII G_rGCy'C
Cuts at: 122 2509 6827 7053 7603 8633 122
Size: 2387 4318 226 550 1030 2078
Fragments arranged by size:
4318 2387 2078 1030 550 226
BbeI G_GCGC'C
Cuts at: 8023 8271 8023
Size: 248 10341
BbsI GAAGACnn'nnnn_
Cuts at: 317 521 1069 1346 1691 2540 2719 3806 5825
Size: 204 548 277 345 849 179 1087 2019
Cuts at: 5825 6295 317
Size: 470 4611
Fragments arranged by size:
4611 2019 1087 849 548 470 345 277
204 179
BbvCI CC'TCA_GC
Cuts at: 3581 6140 3581
Size: 2559 8030
BciVI GTATCCnnnnn_n'
Cuts at: 6780 6801 6850 8487 9248 10589 6780
Size: 21 49 1637 761 1341 6780
Fragments arranged by size:
6780 1637 1341 761 49 21
BclI T'GATC_A
Cuts at: 1734 3741 5144 6678 7439 8109 1734
Size: 2007 1403 1534 761 670 4214
Fragments arranged by size:
4214 2007 1534 1403 761 670
BfrBI ATG'CAT
Cuts at: 836 1530 4877 836
Size: 694 3347 6548
Fragments arranged by size:
6548 3347 694
BglII A'GATC_T
Cuts at: 5989 8104 10141 5989
Size: 2115 2037 6437
Fragments arranged by size:
6437 2115 2037
BlpI GC'TnA_GC
Cuts at: 116 7701 116
Size: 7585 3004
Bme1580I G_kGCm'C
Cuts at: 1154 2702 7533 8214 8307 9363 1154
Size: 1548 4831 681 93 1056 2380
Fragments arranged by size:
4831 2380 1548 1056 681 93
BmrI ACTGGGnnnn_n'
Cuts at: 2532 6995 7414 7801 7882 8217 10361 2532
Size: 4463 419 387 81 335 2144 2760
Fragments arranged by size:
4463 2760 2144 419 387 335 81
BmtI G_CTAG'C
Cuts at: 2314 2314
Size: 10589
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2787 6806 8956 2787
Size: 4019 2150 4420
Fragments arranged by size:
4420 4019 2150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1194 4184 4847 6086 7099 7333 1194
Size: 2990 663 1239 1013 234 4450
Fragments arranged by size:
4450 2990 1239 1013 663 234
Bpu10I CC'TnA_GC
Cuts at: 2240 3167 3581 6140 6608 2240
Size: 927 414 2559 468 6221
Fragments arranged by size:
6221 2559 927 468 414
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1723 2118 5080 5162 5919 7177 7765 8644 9136
Size: 395 2962 82 757 1258 588 879 492
Cuts at: 9136 9434 1723
Size: 298 2878
Fragments arranged by size:
2962 2878 1258 879 757 588 492 395
298 82
BsaI GGTCTCn'nnnn_
Cuts at: 1183 10293 1183
Size: 9110 1479
BsaAI yAC'GTr
Cuts at: 2080 7942 2080
Size: 5862 4727
BsaBI GATnn'nnATC
Cuts at: 6 3740 7309 8103 10140 6
Size: 3734 3569 794 2037 455
Fragments arranged by size:
3734 3569 2037 794 455
BsaHI Gr'CG_yC
Cuts at: 8020 8268 8970 10466 8020
Size: 248 702 1496 8143
Fragments arranged by size:
8143 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1573 7778 8299 8977 9251 9398 9809 1573
Size: 6205 521 678 274 147 411 2353
Fragments arranged by size:
6205 2353 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 192 222 1192 1222 192
Size: 30 970 30 9559
Fragments arranged by size:
9559 970 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 200 1835 2905 4459 4474 4477 4486 4489 4492
Size: 1635 1070 1554 15 3 9 3 3
Cuts at: 4492 4495 4504 4507 5346 200
Size: 3 9 3 839 5443
Fragments arranged by size:
5443 1635 1554 1070 839 15 9 9
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 6096 8014 8772 9349 9845 6096
Size: 1918 758 577 496 6840
Fragments arranged by size:
6840 1918 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 603 4777 5495 6365 172
Size: 431 4174 718 870 4396
Fragments arranged by size:
4396 4174 870 718 431
BsiEI CG_ry'CG
Cuts at: 104 2107 8131 8177 9385 104
Size: 2003 6024 46 1208 1308
Fragments arranged by size:
6024 2003 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1526 2509 5520 6827 7533 7603 8381 8571 9363
Size: 983 3011 1307 706 70 778 190 792
Cuts at: 9363 1526
Size: 2752
Fragments arranged by size:
3011 2752 1307 983 792 778 706 190
70
BsmI GAATG_Cn'
Cuts at: 836 4877 6153 836
Size: 4041 1276 5272
Fragments arranged by size:
5272 4041 1276
BsmBI CGTCTCn'nnnn_
Cuts at: 4608 4608
Size: 10589
Bsp1286I G_dGCh'C
Cuts at: 122 1154 1526 2509 2702 5520 6827 7053 7533
Size: 1032 372 983 193 2818 1307 226 480
Cuts at: 7533 7603 8214 8307 8381 8571 8633 9363 122
Size: 70 611 93 74 190 62 730 1348
Fragments arranged by size:
2818 1348 1307 1032 983 730 611 480
372 226 193 190 93 74 70 62
BspEI T'CCGG_A
Cuts at: 9809 9809
Size: 10589
BspHI T'CATG_A
Cuts at: 162 7415 7442 9765 162
Size: 7253 27 2323 986
Fragments arranged by size:
7253 2323 986 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1632 4749 5701 6678 7639 7853 8155 8536 1632
Size: 3117 952 977 961 214 302 381 3685
Fragments arranged by size:
3685 3117 977 961 952 381 302 214
BsrBI CCG'CTC
Cuts at: 50 8881 8935 10419 10584 50
Size: 8831 54 1484 165 55
Fragments arranged by size:
8831 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 230 679 7067 8501 230
Size: 449 6388 1434 2318
Fragments arranged by size:
6388 2318 1434 449
BsrFI r'CCGG_y
Cuts at: 1573 8587 8768 8977 1573
Size: 7014 181 209 3185
Fragments arranged by size:
7014 3185 209 181
BsrGI T'GTAC_A
Cuts at: 489 489
Size: 10589
BssSI C'ACGA_G
Cuts at: 8860 9218 8860
Size: 358 10231
BstAPI GCAn_nnn'nTGC
Cuts at: 468 833 7966 468
Size: 365 7133 3091
Fragments arranged by size:
7133 3091 365
BstBI TT'CG_AA
Cuts at: 2067 6189 6342 8950 2067
Size: 4122 153 2608 3706
Fragments arranged by size:
4122 3706 2608 153
BstEII G'GTnAC_C
Cuts at: 7408 7408
Size: 10589
BstXI CCAn_nnnn'nTGG
Cuts at: 876 876
Size: 10589
BstYI r'GATC_y
Cuts at: 1 2071 3976 5419 5989 6466 7614 8081 8104
Size: 2070 1905 1443 570 477 1148 467 23
Cuts at: 8104 8439 8685 9697 9783 9795 10141 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2070 1905 1443 1148 1012 570 477 467
449 346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3194 5093 3194
Size: 1899 8690
BtgI C'CryG_G
Cuts at: 5486 6617 5486
Size: 1131 9458
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1378 3044 3785 8539 8720 8883 1378
Size: 1666 741 4754 181 163 3084
Fragments arranged by size:
4754 3084 1666 741 181 163
BtsI GCAGTG_nn'
Cuts at: 620 938 1761 4239 5955 7145 7832 620
Size: 318 823 2478 1716 1190 687 3377
Fragments arranged by size:
3377 2478 1716 1190 823 687 318
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 751 786 751
Size: 35 10554
DraI TTT'AAA
Cuts at: 531 1229 2408 6050 7588 531
Size: 698 1179 3642 1538 3532
Fragments arranged by size:
3642 3532 1538 1179 698
DraIII CAC_nnn'GTG
Cuts at: 4697 4697
Size: 10589
DrdI GACnn_nn'nnGTC
Cuts at: 8295 9153 8295
Size: 858 9731
EaeI y'GGCC_r
Cuts at: 8174 8348 8739 8766 10111 8174
Size: 174 391 27 1345 8652
Fragments arranged by size:
8652 1345 391 174 27
EagI C'GGCC_G
Cuts at: 8174 8174
Size: 10589
EarI CTCTTCn'nnn_
Cuts at: 287 312 2658 2778 3339 3409 3483 4436 4451
Size: 25 2346 120 561 70 74 953 15
Cuts at: 4451 4463 4481 4598 4604 5188 5694 6775 6893
Size: 12 18 117 6 584 506 1081 118
Cuts at: 6893 7095 7260 8612 8822 287
Size: 202 165 1352 210 2054
Fragments arranged by size:
2346 2054 1352 1081 953 584 561 506
210 202 165 120 118 117 74 70
25 18 15 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 8797 9107 9253 10399 8797
Size: 310 146 1146 8987
Fragments arranged by size:
8987 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2507 6825 7601 2507
Size: 4318 776 5495
Fragments arranged by size:
5495 4318 776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 293 311 1194 1658 2339 2678 2759 3062
Size: 78 18 883 464 681 339 81 303
Cuts at: 3062 3077 3095 3320 3614 3824 3965 4112 4184
Size: 15 18 225 294 210 141 147 72
Cuts at: 4184 4847 6086 6425 6814 6929 7099 7333 8414
Size: 663 1239 339 389 115 170 234 1081
Cuts at: 8414 8846 9593 9861 215
Size: 432 747 268 943
Fragments arranged by size:
1239 1081 943 883 747 681 663 464
432 389 339 339 303 294 268 234
225 210 170 147 141 115 81 78
72 18 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2323 4529 7568 2323
Size: 2206 3039 5344
Fragments arranged by size:
5344 3039 2206
EcoO109I rG'GnC_Cy
Cuts at: 535 5242 7083 7561 7728 535
Size: 4707 1841 478 167 3396
Fragments arranged by size:
4707 3396 1841 478 167
EcoRI G'AATT_C
Cuts at: 2627 3747 5313 5982 7593 2627
Size: 1120 1566 669 1611 5623
Fragments arranged by size:
5623 1611 1566 1120 669
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 616 723 1513 3973 6283 8608 616
Size: 107 790 2460 2310 2325 2597
Fragments arranged by size:
2597 2460 2325 2310 790 107
FspI TGC'GCA
Cuts at: 8370 8370
Size: 10589
HaeII r_GCGC'y
Cuts at: 8023 8271 9293 10272 8023
Size: 248 1022 979 8340
Fragments arranged by size:
8340 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 623 655 1645 1677 1768 1800 2268 2300 2635
Size: 32 990 32 91 32 468 32 335
Cuts at: 2635 2667 2934 2966 2974 3006 3271 3303 3415
Size: 32 267 32 8 32 265 32 112
Cuts at: 3415 3447 3694 3726 3961 3993 4144 4176 4504
Size: 32 247 32 235 32 151 32 328
Cuts at: 4504 4536 5845 5877 5917 5949 6431 6463 6621
Size: 32 1309 32 40 32 482 32 158
Cuts at: 6621 6653 7137 7169 7259 7291 7431 7463 8431
Size: 32 484 32 90 32 140 32 968
Cuts at: 8431 8461 8463 8493 623
Size: 30 2 30 2719
Fragments arranged by size:
2719 1309 990 968 484 482 468 335
328 267 265 247 235 158 151 140
112 91 90 40 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
30 30 8 2
HincII GTy'rAC
Cuts at: 1008 3308 6905 7628 1008
Size: 2300 3597 723 3969
Fragments arranged by size:
3969 3597 2300 723
HindIII A'AGCT_T
Cuts at: 157 2875 5056 5225 5719 157
Size: 2718 2181 169 494 5027
Fragments arranged by size:
5027 2718 2181 494 169
KasI G'GCGC_C
Cuts at: 8019 8267 8019
Size: 248 10341
KpnI G_GTAC'C
Cuts at: 7609 7609
Size: 10589
MfeI C'AATT_G
Cuts at: 359 359
Size: 10589
MluI A'CGCG_T
Cuts at: 10022 10022
Size: 10589
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 908 1567 4322 5179 9260 9444 908
Size: 659 2755 857 4081 184 2053
Fragments arranged by size:
4081 2755 2053 857 659 184
MscI TGG'CCA
Cuts at: 8350 8350
Size: 10589
MslI CAynn'nnrTG
Cuts at: 874 1535 5603 6866 8578 8705 874
Size: 661 4068 1263 1712 127 2758
Fragments arranged by size:
4068 2758 1712 1263 661 127
MspA1I CmG'CkG
Cuts at: 115 4308 4356 7077 7174 7316 7700 8014 8374
Size: 4193 48 2721 97 142 384 314 360
Cuts at: 8374 9387 9632 115
Size: 1013 245 1072
Fragments arranged by size:
4193 2721 1072 1013 384 360 314 245
142 97 48
NaeI GCC'GGC
Cuts at: 8770 8770
Size: 10589
NarI GG'CG_CC
Cuts at: 8020 8268 8020
Size: 248 10341
NcoI C'CATG_G
Cuts at: 5486 6617 5486
Size: 1131 9458
NdeI CA'TA_TG
Cuts at: 3366 3366
Size: 10589
NgoMIV G'CCGG_C
Cuts at: 8768 8768
Size: 10589
NheI G'CTAG_C
Cuts at: 2310 2310
Size: 10589
NsiI A_TGCA'T
Cuts at: 838 1532 4879 838
Size: 694 3347 6548
Fragments arranged by size:
6548 3347 694
NspI r_CATG'y
Cuts at: 947 1530 2969 5112 7642 8142 9049 947
Size: 583 1439 2143 2530 500 907 2487
Fragments arranged by size:
2530 2487 2143 1439 907 583 500
PciI A'CATG_T
Cuts at: 2965 9045 2965
Size: 6080 4509
PflMI CCAn_nnn'nTGG
Cuts at: 4893 7498 4893
Size: 2605 7984
PfoI T'CCnGG_A
Cuts at: 4524 7557 4524
Size: 3033 7556
PmeI GTTT'AAAC
Cuts at: 7588 7588
Size: 10589
PmlI CAC'GTG
Cuts at: 2080 2080
Size: 10589
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2331 2363 9728 9760 2331
Size: 32 7365 32 3160
Fragments arranged by size:
7365 3160 32 32
PpuMI rG'GwC_Cy
Cuts at: 7083 7561 7083
Size: 478 10111
PsiI TTA'TAA
Cuts at: 5449 6320 5449
Size: 871 9718
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4793 4825 4793
Size: 32 10557
PstI C_TGCA'G
Cuts at: 5117 7636 8321 5117
Size: 2519 685 7385
Fragments arranged by size:
7385 2519 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10589
PvuII CAG'CTG
Cuts at: 7077 7174 7316 8014 8374 7077
Size: 97 142 698 360 9292
Fragments arranged by size:
9292 698 360 142 97
RsrII CG'GwC_CG
Cuts at: 8784 8784
Size: 10589
SacI G_AGCT'C
Cuts at: 2509 6827 7603 2509
Size: 4318 776 5495
Fragments arranged by size:
5495 4318 776
SalI G'TCGA_C
Cuts at: 7626 7626
Size: 10589
SapI GCTCTTCn'nnn_
Cuts at: 312 8612 8822 312
Size: 8300 210 2079
Fragments arranged by size:
8300 2079 210
SbfI CC_TGCA'GG
Cuts at: 7636 7636
Size: 10589
ScaI AGT'ACT
Cuts at: 5653 10106 5653
Size: 4453 6136
SfcI C'TryA_G
Cuts at: 33 1841 2228 3752 5113 7632 8317 9310 9501
Size: 1808 387 1524 1361 2519 685 993 191
Cuts at: 9501 33
Size: 1121
Fragments arranged by size:
2519 1808 1524 1361 1121 993 685 387
191
SfoI GGC'GCC
Cuts at: 8021 8269 8021
Size: 248 10341
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10589
SmaI CCC'GGG
Cuts at: 7611 7611
Size: 10589
SmlI C'TyrA_G
Cuts at: 1282 1702 2097 4597 5059 5177 5934 7156 7744
Size: 420 395 2500 462 118 757 1222 588
Cuts at: 7744 8659 9151 9413 1282
Size: 915 492 262 2458
Fragments arranged by size:
2500 2458 1222 915 757 588 492 462
420 395 262 118
SpeI A'CTAG_T
Cuts at: 67 1066 67
Size: 999 9590
SphI G_CATG'C
Cuts at: 7642 8142 7642
Size: 500 10089
SspI AAT'ATT
Cuts at: 1800 3451 5216 1800
Size: 1651 1765 7173
Fragments arranged by size:
7173 1765 1651
StyI C'CwwG_G
Cuts at: 849 4899 5486 5764 6617 7569 7723 8077 849
Size: 4050 587 278 853 952 154 354 3361
Fragments arranged by size:
4050 3361 952 853 587 354 278 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 929 2121 4704 8172 8305 8764 8973 929
Size: 1192 2583 3468 133 459 209 2545
Fragments arranged by size:
3468 2583 2545 1192 459 209 133
TatI w'GTAC_w
Cuts at: 489 2976 5651 7212 7358 10104 489
Size: 2487 2675 1561 146 2746 974
Fragments arranged by size:
2746 2675 2487 1561 974 146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4683 4715 5174 5206 10106 10138 4683
Size: 32 459 32 4900 32 5134
Fragments arranged by size:
5134 4900 459 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 934 8386 934
Size: 7452 3137
XbaI T'CTAG_A
Cuts at: 7620 7620
Size: 10589
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4801 6104 8084 4801
Size: 1303 1980 7306
Fragments arranged by size:
7306 1980 1303
XmaI C'CCGG_G
Cuts at: 7609 7609
Size: 10589
XmnI GAAnn'nnTTC
Cuts at: 2454 3405 5625 6185 6263 2454
Size: 951 2220 560 78 6780
Fragments arranged by size:
6780 2220 951 560 78
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AleI AloI ApaI AscI AvrII BaeI
BcgI BglI BmgBI BsiWI BssHII BstZ17I ClaI EcoRV
FseI FspAI HpaI NotI NruI PacI PasI PshAI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI