Restriction map for
pF1KE9543
(Circular) (Six-base) MAPSORT of: pF1KE9543.seq Check: 6058 from: 1 to: 4601
pF1KE9543 4601 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 1639 1639
Size: 4601
Acc65I G'GTAC_C
Cuts at: 1617 1617
Size: 4601
AclI AA'CG_TT
Cuts at: 4441 4441
Size: 4601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1137 2426 2858 3605 3873 1096
Size: 41 1289 432 747 268 1824
Fragments arranged by size:
1824 1289 747 432 268 41
AflIII A'CryG_T
Cuts at: 292 1953 3057 4034 292
Size: 1661 1104 977 859
Fragments arranged by size:
1661 1104 977 859
AgeI A'CCGG_T
Cuts at: 2989 2989
Size: 4601
AleI CACnn'nnGTG
Cuts at: 812 812
Size: 4601
AlwNI CAG_nnn'CTG
Cuts at: 1906 3473 1906
Size: 1567 3034
ApaLI G'TGCA_C
Cuts at: 121 3371 121
Size: 3250 1351
ApoI r'AATT_y
Cuts at: 1605 3954 4210 1605
Size: 2349 256 1996
Fragments arranged by size:
2349 1996 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4601
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
AvaI C'yCGr_G
Cuts at: 1621 1621
Size: 4601
BamHI G'GATC_C
Cuts at: 1626 1626
Size: 4601
BanI G'GyrC_C
Cuts at: 1519 1617 2031 2279 2314 1519
Size: 98 414 248 35 3806
Fragments arranged by size:
3806 414 248 98 35
BanII G_rGCy'C
Cuts at: 347 1194 1360 1615 2645 347
Size: 847 166 255 1030 2303
Fragments arranged by size:
2303 1030 847 255 166
BbeI G_GCGC'C
Cuts at: 2035 2283 2035
Size: 248 4353
BbsI GAAGACnn'nnnn_
Cuts at: 708 708
Size: 4601
BbvCI CC'TCA_GC
Cuts at: 700 700
Size: 4601
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 582 616 582
Size: 34 4567
BciVI GTATCCnnnnn_n'
Cuts at: 2499 3260 4601 2499
Size: 761 1341 2499
Fragments arranged by size:
2499 1341 761
BclI T'GATC_A
Cuts at: 1031 2121 1031
Size: 1090 3511
BfrBI ATG'CAT
Cuts at: 171 171
Size: 4601
BglII A'GATC_T
Cuts at: 426 2116 4153 426
Size: 1690 2037 874
Fragments arranged by size:
2037 1690 874
BlpI GC'TnA_GC
Cuts at: 1193 1713 1193
Size: 520 4081
Bme1580I G_kGCm'C
Cuts at: 125 1522 2226 2319 3375 125
Size: 1397 704 93 1056 1351
Fragments arranged by size:
1397 1351 1056 704 93
BmrI ACTGGGnnnn_n'
Cuts at: 545 1813 1894 2229 4373 545
Size: 1268 81 335 2144 773
Fragments arranged by size:
2144 1268 773 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2968 2968
Size: 4601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 245 1103 232
Size: 13 858 3730
Fragments arranged by size:
3730 858 13
Bpu10I CC'TnA_GC
Cuts at: 341 700 1537 341
Size: 359 837 3405
Fragments arranged by size:
3405 837 359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1777 2656 3148 3446 1777
Size: 879 492 298 2932
Fragments arranged by size:
2932 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 373 871 4305 373
Size: 498 3434 669
Fragments arranged by size:
3434 669 498
BsaAI yAC'GTr
Cuts at: 293 1954 293
Size: 1661 2940
BsaBI GATnn'nnATC
Cuts at: 6 2115 4152 6
Size: 2109 2037 455
Fragments arranged by size:
2109 2037 455
BsaHI Gr'CG_yC
Cuts at: 2032 2280 2982 4478 2032
Size: 248 702 1496 2155
Fragments arranged by size:
2155 1496 702 248
BsaWI w'CCGG_w
Cuts at: 641 1790 2311 2989 3263 3410 3821 641
Size: 1149 521 678 274 147 411 1421
Fragments arranged by size:
1421 1149 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 403 433 403
Size: 30 4571
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 133
Size: 4601
BseYI C'CCAG_C
Cuts at: 926 995 1347 1353 1523 2026 2784 3361 3857
Size: 69 352 6 170 503 758 577 496
Cuts at: 3857 926
Size: 1670
Fragments arranged by size:
1670 758 577 503 496 352 170 69
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 444 634 639 1123 1183 444
Size: 190 5 484 60 3862
Fragments arranged by size:
3862 484 190 60 5
BsiEI CG_ry'CG
Cuts at: 104 2143 2189 3397 104
Size: 2039 46 1208 1308
Fragments arranged by size:
2039 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 125 347 1615 2393 2583 3375 125
Size: 222 1268 778 190 792 1351
Fragments arranged by size:
1351 1268 792 778 222 190
BsmI GAATG_Cn'
Cuts at: 171 171
Size: 4601
BsmBI CGTCTCn'nnnn_
Cuts at: 1496 1496
Size: 4601
Bsp1286I G_dGCh'C
Cuts at: 125 347 1194 1360 1522 1615 2226 2319 2393
Size: 222 847 166 162 93 611 93 74
Cuts at: 2393 2583 2645 3375 125
Size: 190 62 730 1351
Fragments arranged by size:
1351 847 730 611 222 190 166 162
93 93 74 62
BspEI T'CCGG_A
Cuts at: 641 3821 641
Size: 3180 1421
BspHI T'CATG_A
Cuts at: 3777 3777
Size: 4601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1651 1865 2167 2548 1651
Size: 214 302 381 3704
Fragments arranged by size:
3704 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2893 2947 4431 4596 50
Size: 2843 54 1484 165 55
Fragments arranged by size:
2843 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1140 2513 1140
Size: 1373 3228
BsrFI r'CCGG_y
Cuts at: 2599 2780 2989 2599
Size: 181 209 4211
Fragments arranged by size:
4211 209 181
BssSI C'ACGA_G
Cuts at: 1336 2872 3230 1336
Size: 1536 358 2707
Fragments arranged by size:
2707 1536 358
BstAPI GCAn_nnn'nTGC
Cuts at: 761 1978 761
Size: 1217 3384
BstBI TT'CG_AA
Cuts at: 2962 2962
Size: 4601
BstXI CCAn_nnnn'nTGG
Cuts at: 210 621 210
Size: 411 4190
BstYI r'GATC_y
Cuts at: 1 218 426 1626 2093 2116 2451 2697 3709
Size: 217 208 1200 467 23 335 246 1012
Cuts at: 3709 3795 3807 4153 1
Size: 86 12 346 449
Fragments arranged by size:
1200 1012 467 449 346 335 246 217
208 86 23 12
BtgI C'CryG_G
Cuts at: 107 620 107
Size: 513 4088
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2551 2732 2895 2551
Size: 181 163 4257
Fragments arranged by size:
4257 181 163
BtsI GCAGTG_nn'
Cuts at: 614 651 759 1509 1844 614
Size: 37 108 750 335 3371
Fragments arranged by size:
3371 750 335 108 37
DraI TTT'AAA
Cuts at: 1600 1600
Size: 4601
DrdI GACnn_nn'nnGTC
Cuts at: 2307 3165 2307
Size: 858 3743
EaeI y'GGCC_r
Cuts at: 1496 1589 2186 2360 2751 2778 4123 1496
Size: 93 597 174 391 27 1345 1974
Fragments arranged by size:
1974 1345 597 391 174 93 27
EagI C'GGCC_G
Cuts at: 2186 2186
Size: 4601
EarI CTCTTCn'nnn_
Cuts at: 352 865 2624 2834 352
Size: 513 1759 210 2119
Fragments arranged by size:
2119 1759 513 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2809 3119 3265 4411 2809
Size: 310 146 1146 2999
Fragments arranged by size:
2999 1146 310 146
EcoICRI GAG'CTC
Cuts at: 345 1613 345
Size: 1268 3333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 245 1096 1103 1137 2426 2858 3605 3873
Size: 13 851 7 34 1289 432 747 268
Cuts at: 3873 232
Size: 960
Fragments arranged by size:
1289 960 851 747 432 268 34 13
7
EcoO109I rG'GnC_Cy
Cuts at: 570 849 932 1039 1740 570
Size: 279 83 107 701 3431
Fragments arranged by size:
3431 701 279 107 83
EcoRI G'AATT_C
Cuts at: 1605 1605
Size: 4601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4601
FspI TGC'GCA
Cuts at: 2382 2382
Size: 4601
HaeII r_GCGC'y
Cuts at: 2035 2283 3305 4284 2035
Size: 248 1022 979 2352
Fragments arranged by size:
2352 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 403 435 460 492 2443 2473 2475 2505 403
Size: 32 25 32 1951 30 2 30 2499
Fragments arranged by size:
2499 1951 32 32 30 30 25 2
HincII GTy'rAC
Cuts at: 1065 1640 1065
Size: 575 4026
HpaI GTT'AAC
Cuts at: 1065 1065
Size: 4601
KasI G'GCGC_C
Cuts at: 2031 2279 2031
Size: 248 4353
KpnI G_GTAC'C
Cuts at: 1621 1621
Size: 4601
MluI A'CGCG_T
Cuts at: 4034 4034
Size: 4601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 277 458 848 3272 3456 277
Size: 181 390 2424 184 1422
Fragments arranged by size:
2424 1422 390 184 181
MscI TGG'CCA
Cuts at: 1498 1591 2362 1498
Size: 93 771 3737
Fragments arranged by size:
3737 771 93
MslI CAynn'nnrTG
Cuts at: 314 442 619 673 812 1099 2590 2717 314
Size: 128 177 54 139 287 1491 127 2198
Fragments arranged by size:
2198 1491 287 177 139 128 127 54
MspA1I CmG'CkG
Cuts at: 335 1157 1188 1712 2026 2386 3399 3644 335
Size: 822 31 524 314 360 1013 245 1292
Fragments arranged by size:
1292 1013 822 524 360 314 245 31
NaeI GCC'GGC
Cuts at: 2782 2782
Size: 4601
NarI GG'CG_CC
Cuts at: 2032 2280 2032
Size: 248 4353
NcoI C'CATG_G
Cuts at: 107 620 107
Size: 513 4088
NgoMIV G'CCGG_C
Cuts at: 2780 2780
Size: 4601
NsiI A_TGCA'T
Cuts at: 173 173
Size: 4601
NspI r_CATG'y
Cuts at: 1566 1654 2154 3061 1566
Size: 88 500 907 3106
Fragments arranged by size:
3106 907 500 88
PciI A'CATG_T
Cuts at: 3057 3057
Size: 4601
PmeI GTTT'AAAC
Cuts at: 1600 1600
Size: 4601
PmlI CAC'GTG
Cuts at: 293 293
Size: 4601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 4569
PpuMI rG'GwC_Cy
Cuts at: 932 932
Size: 4601
PshAI GACnn'nnGTC
Cuts at: 1488 1488
Size: 4601
PstI C_TGCA'G
Cuts at: 1648 2333 1648
Size: 685 3916
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4601
PvuII CAG'CTG
Cuts at: 335 1157 2026 2386 335
Size: 822 869 360 2550
Fragments arranged by size:
2550 869 822 360
RsrII CG'GwC_CG
Cuts at: 2796 2796
Size: 4601
SacI G_AGCT'C
Cuts at: 347 1615 347
Size: 1268 3333
SalI G'TCGA_C
Cuts at: 1638 1638
Size: 4601
SanDI GG'GwC_CC
Cuts at: 932 932
Size: 4601
SapI GCTCTTCn'nnn_
Cuts at: 352 2624 2834 352
Size: 2272 210 2119
Fragments arranged by size:
2272 2119 210
SbfI CC_TGCA'GG
Cuts at: 1648 1648
Size: 4601
ScaI AGT'ACT
Cuts at: 4118 4118
Size: 4601
SfcI C'TryA_G
Cuts at: 33 588 1644 2329 3322 3513 33
Size: 555 1056 685 993 191 1121
Fragments arranged by size:
1121 1056 993 685 555 191
SfoI GGC'GCC
Cuts at: 2033 2281 2033
Size: 248 4353
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
SmaI CCC'GGG
Cuts at: 1623 1623
Size: 4601
SmlI C'TyrA_G
Cuts at: 1756 2671 3163 3425 1756
Size: 915 492 262 2932
Fragments arranged by size:
2932 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4601
SphI G_CATG'C
Cuts at: 1654 2154 1654
Size: 500 4101
StyI C'CwwG_G
Cuts at: 107 620 1735 2089 107
Size: 513 1115 354 2619
Fragments arranged by size:
2619 1115 513 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 304 397 1017 1537 2184 2317 2776 2985 304
Size: 93 620 520 647 133 459 209 1920
Fragments arranged by size:
1920 647 620 520 459 209 133 93
TatI w'GTAC_w
Cuts at: 4116 4116
Size: 4601
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 4569
Tth111I GACn'n_nGTC
Cuts at: 370 2398 370
Size: 2028 2573
XbaI T'CTAG_A
Cuts at: 1632 1632
Size: 4601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 237 371 2096 237
Size: 134 1725 2742
Fragments arranged by size:
2742 1725 134
XmaI C'CCGG_G
Cuts at: 1621 1621
Size: 4601
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PmlI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BglI BmgBI BmtI BsiWI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI