Restriction map for
pF1KE9279
(Circular) (Six-base) MAPSORT of: pF1KE9279.seq Check: 342 from: 1 to: 3983
pF1KE9279 3983 bp
With 182 enzymes: SgfI *
March 29, 2013 15:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 132 132
Size: 3983
AccI GT'mk_AC
Cuts at: 1021 1021
Size: 3983
Acc65I G'GTAC_C
Cuts at: 999 999
Size: 3983
AclI AA'CG_TT
Cuts at: 3823 3823
Size: 3983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1808 2240 2987 3255 1808
Size: 432 747 268 2536
Fragments arranged by size:
2536 747 432 268
AflIII A'CryG_T
Cuts at: 1335 2439 3416 1335
Size: 1104 977 1902
Fragments arranged by size:
1902 1104 977
AgeI A'CCGG_T
Cuts at: 2371 2371
Size: 3983
AlwNI CAG_nnn'CTG
Cuts at: 834 1288 2855 834
Size: 454 1567 1962
Fragments arranged by size:
1962 1567 454
ApaLI G'TGCA_C
Cuts at: 2753 2753
Size: 3983
ApoI r'AATT_y
Cuts at: 987 3336 3592 987
Size: 2349 256 1378
Fragments arranged by size:
2349 1378 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3983
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
AvaI C'yCGr_G
Cuts at: 177 1003 177
Size: 826 3157
BamHI G'GATC_C
Cuts at: 1008 1008
Size: 3983
BanI G'GyrC_C
Cuts at: 999 1413 1661 1696 999
Size: 414 248 35 3286
Fragments arranged by size:
3286 414 248 35
BanII G_rGCy'C
Cuts at: 997 2027 997
Size: 1030 2953
BbeI G_GCGC'C
Cuts at: 1417 1665 1417
Size: 248 3735
BbsI GAAGACnn'nnnn_
Cuts at: 633 633
Size: 3983
BciVI GTATCCnnnnn_n'
Cuts at: 1881 2642 3983 1881
Size: 761 1341 1881
Fragments arranged by size:
1881 1341 761
BclI T'GATC_A
Cuts at: 1503 1503
Size: 3983
BglII A'GATC_T
Cuts at: 1498 3535 1498
Size: 2037 1946
BlpI GC'TnA_GC
Cuts at: 1095 1095
Size: 3983
Bme1580I G_kGCm'C
Cuts at: 1608 1701 2757 1608
Size: 93 1056 2834
Fragments arranged by size:
2834 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 635 1195 1276 1611 3755 635
Size: 560 81 335 2144 863
Fragments arranged by size:
2144 863 560 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 3983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 2038 2530 2828 1159
Size: 879 492 298 2314
Fragments arranged by size:
2314 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3687 3687
Size: 3983
BsaAI yAC'GTr
Cuts at: 816 1336 816
Size: 520 3463
BsaBI GATnn'nnATC
Cuts at: 6 1497 3534 6
Size: 1491 2037 455
Fragments arranged by size:
2037 1491 455
BsaHI Gr'CG_yC
Cuts at: 1414 1662 2364 3860 1414
Size: 248 702 1496 1537
Fragments arranged by size:
1537 1496 702 248
BsaWI w'CCGG_w
Cuts at: 566 1172 1693 2371 2645 2792 3203 566
Size: 606 521 678 274 147 411 1346
Fragments arranged by size:
1346 678 606 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 154 184 154
Size: 30 3953
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 220 166
Size: 54 3929
BseYI C'CCAG_C
Cuts at: 215 413 572 576 709 1408 2166 2743 3239
Size: 198 159 4 133 699 758 577 496
Cuts at: 3239 215
Size: 959
Fragments arranged by size:
959 758 699 577 496 198 159 133
4
BsiEI CG_ry'CG
Cuts at: 104 945 1525 1571 2779 104
Size: 841 580 46 1208 1308
Fragments arranged by size:
1308 1208 841 580 46
BsiHKAI G_wGCw'C
Cuts at: 997 1775 1965 2757 997
Size: 778 190 792 2223
Fragments arranged by size:
2223 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 997 1608 1701 1775 1965 2027 2757 997
Size: 611 93 74 190 62 730 2223
Fragments arranged by size:
2223 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3203 3203
Size: 3983
BspHI T'CATG_A
Cuts at: 3159 3159
Size: 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 132 1033 1247 1549 1930 132
Size: 901 214 302 381 2185
Fragments arranged by size:
2185 901 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2275 2329 3813 3978 50
Size: 2225 54 1484 165 55
Fragments arranged by size:
2225 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1895 1895
Size: 3983
BsrFI r'CCGG_y
Cuts at: 940 1981 2162 2371 940
Size: 1041 181 209 2552
Fragments arranged by size:
2552 1041 209 181
BssSI C'ACGA_G
Cuts at: 2254 2612 2254
Size: 358 3625
BstAPI GCAn_nnn'nTGC
Cuts at: 1360 1360
Size: 3983
BstBI TT'CG_AA
Cuts at: 2344 2344
Size: 3983
BstYI r'GATC_y
Cuts at: 1 1008 1475 1498 1833 2079 3091 3177 3189
Size: 1007 467 23 335 246 1012 86 12
Cuts at: 3189 3535 1
Size: 346 449
Fragments arranged by size:
1012 1007 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3983
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 894 1933 2114 2277 894
Size: 1039 181 163 2600
Fragments arranged by size:
2600 1039 181 163
BtsI GCAGTG_nn'
Cuts at: 1226 1226
Size: 3983
DraI TTT'AAA
Cuts at: 982 982
Size: 3983
DraIII CAC_nnn'GTG
Cuts at: 284 284
Size: 3983
DrdI GACnn_nn'nnGTC
Cuts at: 1689 2547 1689
Size: 858 3125
EaeI y'GGCC_r
Cuts at: 338 942 1568 1742 2133 2160 3505 338
Size: 604 626 174 391 27 1345 816
Fragments arranged by size:
1345 816 626 604 391 174 27
EagI C'GGCC_G
Cuts at: 942 1568 942
Size: 626 3357
EarI CTCTTCn'nnn_
Cuts at: 124 238 2006 2216 124
Size: 114 1768 210 1891
Fragments arranged by size:
1891 1768 210 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2191 2501 2647 3793 2191
Size: 310 146 1146 2381
Fragments arranged by size:
2381 1146 310 146
EcoICRI GAG'CTC
Cuts at: 995 995
Size: 3983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1808 2240 2987 3255 1808
Size: 432 747 268 2536
Fragments arranged by size:
2536 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1122 1122
Size: 3983
EcoRI G'AATT_C
Cuts at: 987 987
Size: 3983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2002 2002
Size: 3983
FspI TGC'GCA
Cuts at: 1764 1764
Size: 3983
HaeII r_GCGC'y
Cuts at: 507 1417 1665 2687 3666 507
Size: 910 248 1022 979 824
Fragments arranged by size:
1022 979 910 824 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1825 1855 1857 1887 1825
Size: 30 2 30 3921
Fragments arranged by size:
3921 30 30 2
HincII GTy'rAC
Cuts at: 1022 1022
Size: 3983
KasI G'GCGC_C
Cuts at: 1413 1661 1413
Size: 248 3735
KpnI G_GTAC'C
Cuts at: 1003 1003
Size: 3983
MluI A'CGCG_T
Cuts at: 3416 3416
Size: 3983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 332 2654 2838 332
Size: 2322 184 1477
Fragments arranged by size:
2322 1477 184
MscI TGG'CCA
Cuts at: 340 1744 340
Size: 1404 2579
MslI CAynn'nnrTG
Cuts at: 265 286 649 1972 2099 265
Size: 21 363 1323 127 2149
Fragments arranged by size:
2149 1323 363 127 21
MspA1I CmG'CkG
Cuts at: 215 576 1094 1408 1768 2781 3026 215
Size: 361 518 314 360 1013 245 1172
Fragments arranged by size:
1172 1013 518 361 360 314 245
NaeI GCC'GGC
Cuts at: 942 2164 942
Size: 1222 2761
NarI GG'CG_CC
Cuts at: 1414 1662 1414
Size: 248 3735
NcoI C'CATG_G
Cuts at: 107 107
Size: 3983
NgoMIV G'CCGG_C
Cuts at: 940 2162 940
Size: 1222 2761
NspI r_CATG'y
Cuts at: 427 1036 1536 2443 427
Size: 609 500 907 1967
Fragments arranged by size:
1967 907 609 500
PasI CC'CwG_GG
Cuts at: 499 499
Size: 3983
PciI A'CATG_T
Cuts at: 2439 2439
Size: 3983
PflMI CCAn_nnn'nTGG
Cuts at: 195 526 195
Size: 331 3652
PmeI GTTT'AAAC
Cuts at: 982 982
Size: 3983
PmlI CAC'GTG
Cuts at: 816 816
Size: 3983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3122 3154 3122
Size: 32 3951
PstI C_TGCA'G
Cuts at: 299 1030 1715 299
Size: 731 685 2567
Fragments arranged by size:
2567 731 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3983
PvuII CAG'CTG
Cuts at: 215 576 1408 1768 215
Size: 361 832 360 2430
Fragments arranged by size:
2430 832 361 360
RsrII CG'GwC_CG
Cuts at: 2178 2178
Size: 3983
SacI G_AGCT'C
Cuts at: 997 997
Size: 3983
SalI G'TCGA_C
Cuts at: 1020 1020
Size: 3983
SapI GCTCTTCn'nnn_
Cuts at: 124 2006 2216 124
Size: 1882 210 1891
Fragments arranged by size:
1891 1882 210
SbfI CC_TGCA'GG
Cuts at: 1030 1030
Size: 3983
ScaI AGT'ACT
Cuts at: 3500 3500
Size: 3983
SfcI C'TryA_G
Cuts at: 33 295 1026 1711 2704 2895 33
Size: 262 731 685 993 191 1121
Fragments arranged by size:
1121 993 731 685 262 191
SfoI GGC'GCC
Cuts at: 1415 1663 1415
Size: 248 3735
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
SmaI CCC'GGG
Cuts at: 1005 1005
Size: 3983
SmlI C'TyrA_G
Cuts at: 1138 2053 2545 2807 1138
Size: 915 492 262 2314
Fragments arranged by size:
2314 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3983
SphI G_CATG'C
Cuts at: 1036 1536 1036
Size: 500 3483
StyI C'CwwG_G
Cuts at: 107 1117 1471 107
Size: 1010 354 2619
Fragments arranged by size:
2619 1010 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 276 347 622 1566 1699 2158 2367 276
Size: 71 275 944 133 459 209 1892
Fragments arranged by size:
1892 944 459 275 209 133 71
TatI w'GTAC_w
Cuts at: 785 3498 785
Size: 2713 1270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 154 186 3500 3532 154
Size: 32 3314 32 605
Fragments arranged by size:
3314 605 32 32
Tth111I GACn'n_nGTC
Cuts at: 1780 1780
Size: 3983
XbaI T'CTAG_A
Cuts at: 1014 1014
Size: 3983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1478 1478
Size: 3983
XmaI C'CCGG_G
Cuts at: 1003 1003
Size: 3983
XmnI GAAnn'nnTTC
Cuts at: 163 163
Size: 3983
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI