Restriction map for
pF1KE9260
(Circular) (Six-base) MAPSORT of: pF1KE9260.seq Check: 9581 from: 1 to: 7859
pF1KE9260 7859 bp
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AatII G_ACGT'C
Cuts at: 714 4700 714
Size: 3986 3873
AccI GT'mk_AC
Cuts at: 4195 4897 4195
Size: 702 7157
Acc65I G'GTAC_C
Cuts at: 4875 4875
Size: 7859
AclI AA'CG_TT
Cuts at: 7699 7699
Size: 7859
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1438 1595 2695 3491 3601 3963 4126 5684
Size: 788 157 1100 796 110 362 163 1558
Cuts at: 5684 6116 6863 7131 650
Size: 432 747 268 1378
Fragments arranged by size:
1558 1378 1100 796 788 747 432 362
268 163 157 110
AflIII A'CryG_T
Cuts at: 3572 3787 5211 6315 7292 3572
Size: 215 1424 1104 977 4139
Fragments arranged by size:
4139 1424 1104 977 215
AgeI A'CCGG_T
Cuts at: 6247 6247
Size: 7859
AhdI GACnn_n'nnGTC
Cuts at: 4537 4696 4537
Size: 159 7700
AlwNI CAG_nnn'CTG
Cuts at: 628 738 4677 5164 6731 628
Size: 110 3939 487 1567 1756
Fragments arranged by size:
3939 1756 1567 487 110
ApaI G_GGCC'C
Cuts at: 1987 4761 1987
Size: 2774 5085
ApaLI G'TGCA_C
Cuts at: 6629 6629
Size: 7859
ApoI r'AATT_y
Cuts at: 927 1464 2231 2525 2729 3139 3197 3916 3968
Size: 537 767 294 204 410 58 719 52
Cuts at: 3968 4051 4863 7212 7468 927
Size: 83 812 2349 256 1318
Fragments arranged by size:
2349 1318 812 767 719 537 410 294
256 204 83 58 52
AseI AT'TA_AT
Cuts at: 20 20
Size: 7859
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7859
AvaI C'yCGr_G
Cuts at: 1706 3368 4879 1706
Size: 1662 1511 4686
Fragments arranged by size:
4686 1662 1511
BamHI G'GATC_C
Cuts at: 2538 4884 2538
Size: 2346 5513
BanI G'GyrC_C
Cuts at: 173 330 4497 4787 4875 5289 5537 5572 173
Size: 157 4167 290 88 414 248 35 2460
Fragments arranged by size:
4167 2460 414 290 248 157 88 35
BanII G_rGCy'C
Cuts at: 320 1921 1987 3725 4334 4761 4873 5903 320
Size: 1601 66 1738 609 427 112 1030 2276
Fragments arranged by size:
2276 1738 1601 1030 609 427 112 66
BbeI G_GCGC'C
Cuts at: 4791 5293 5541 4791
Size: 502 248 7109
Fragments arranged by size:
7109 502 248
BbsI GAAGACnn'nnnn_
Cuts at: 988 1734 1877 3479 3591 4695 988
Size: 746 143 1602 112 1104 4152
Fragments arranged by size:
4152 1602 1104 746 143 112
BbvCI CC'TCA_GC
Cuts at: 4136 4136
Size: 7859
BciVI GTATCCnnnnn_n'
Cuts at: 809 5757 6518 7859 809
Size: 4948 761 1341 809
Fragments arranged by size:
4948 1341 809 761
BclI T'GATC_A
Cuts at: 5379 5379
Size: 7859
BglI GCCn_nnn'nGGC
Cuts at: 2930 4488 2930
Size: 1558 6301
BglII A'GATC_T
Cuts at: 792 864 2176 5374 7411 792
Size: 72 1312 3198 2037 1240
Fragments arranged by size:
3198 2037 1312 1240 72
BlpI GC'TnA_GC
Cuts at: 4333 4971 4333
Size: 638 7221
Bme1580I G_kGCm'C
Cuts at: 176 333 622 1502 1987 4761 5484 5577 6633
Size: 157 289 880 485 2774 723 93 1056
Cuts at: 6633 176
Size: 1402
Fragments arranged by size:
2774 1402 1056 880 723 485 289 157
93
BmrI ACTGGGnnnn_n'
Cuts at: 5071 5152 5487 7631 5071
Size: 81 335 2144 5299
Fragments arranged by size:
5299 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 921 1935 6226 921
Size: 1014 4291 2554
Fragments arranged by size:
4291 2554 1014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 685 1969 2416 4418 4469 626
Size: 59 1284 447 2002 51 4016
Fragments arranged by size:
4016 2002 1284 447 59 51
Bpu10I CC'TnA_GC
Cuts at: 4136 4136
Size: 7859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 893 1945 2008 4387 5035 5914 6406 6704
Size: 112 1052 63 2379 648 879 492 298
Cuts at: 6704 781
Size: 1936
Fragments arranged by size:
2379 1936 1052 879 648 492 298 112
63
BsaI GGTCTCn'nnnn_
Cuts at: 2384 7563 2384
Size: 5179 2680
BsaAI yAC'GTr
Cuts at: 5212 5212
Size: 7859
BsaBI GATnn'nnATC
Cuts at: 6 394 863 5373 7410 6
Size: 388 469 4510 2037 455
Fragments arranged by size:
4510 2037 469 455 388
BsaHI Gr'CG_yC
Cuts at: 711 4697 4788 5290 5538 6240 7736 711
Size: 3986 91 502 248 702 1496 834
Fragments arranged by size:
3986 1496 834 702 502 248 91
BsaWI w'CCGG_w
Cuts at: 1889 5048 5569 6247 6521 6668 7079 1889
Size: 3159 521 678 274 147 411 2669
Fragments arranged by size:
3159 2669 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2981 3011 3817 3847 2981
Size: 30 806 30 6993
Fragments arranged by size:
6993 806 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 228 859 1927 228
Size: 631 1068 6160
Fragments arranged by size:
6160 1068 631
BseYI C'CCAG_C
Cuts at: 1980 4589 5284 6042 6619 7115 1980
Size: 2609 695 758 577 496 2724
Fragments arranged by size:
2724 2609 758 695 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1181 1181
Size: 7859
BsiEI CG_ry'CG
Cuts at: 104 5401 5447 6655 104
Size: 5297 46 1208 1308
Fragments arranged by size:
5297 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 320 4334 4873 5651 5841 6633 320
Size: 4014 539 778 190 792 1546
Fragments arranged by size:
4014 1546 792 778 539 190
BsmI GAATG_Cn'
Cuts at: 1899 1899
Size: 7859
BsmBI CGTCTCn'nnnn_
Cuts at: 718 1571 718
Size: 853 7006
Bsp1286I G_dGCh'C
Cuts at: 176 320 333 622 1502 1921 1987 3725 4334
Size: 144 13 289 880 419 66 1738 609
Cuts at: 4334 4761 4873 5484 5577 5651 5841 5903 6633
Size: 427 112 611 93 74 190 62 730
Cuts at: 6633 176
Size: 1402
Fragments arranged by size:
1738 1402 880 730 611 609 427 419
289 190 144 112 93 74 66 62
13
BspEI T'CCGG_A
Cuts at: 1889 7079 1889
Size: 5190 2669
BspHI T'CATG_A
Cuts at: 975 2294 2474 7035 975
Size: 1319 180 4561 1799
Fragments arranged by size:
4561 1799 1319 180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3018 3784 4909 5123 5425 5806 3018
Size: 766 1125 214 302 381 5071
Fragments arranged by size:
5071 1125 766 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6151 6205 7689 7854 50
Size: 6101 54 1484 165 55
Fragments arranged by size:
6101 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 803 854 1842 5771 803
Size: 51 988 3929 2891
Fragments arranged by size:
3929 2891 988 51
BsrFI r'CCGG_y
Cuts at: 2772 5857 6038 6247 2772
Size: 3085 181 209 4384
Fragments arranged by size:
4384 3085 209 181
BsrGI T'GTAC_A
Cuts at: 2314 2314
Size: 7859
BssSI C'ACGA_G
Cuts at: 3629 6130 6488 3629
Size: 2501 358 5000
Fragments arranged by size:
5000 2501 358
BstAPI GCAn_nnn'nTGC
Cuts at: 880 1545 5236 880
Size: 665 3691 3503
Fragments arranged by size:
3691 3503 665
BstBI TT'CG_AA
Cuts at: 543 999 2235 6220 543
Size: 456 1236 3985 2182
Fragments arranged by size:
3985 2182 1236 456
BstXI CCAn_nnnn'nTGG
Cuts at: 2223 4164 2223
Size: 1941 5918
BstYI r'GATC_y
Cuts at: 1 240 634 659 792 864 1403 2176 2538
Size: 239 394 25 133 72 539 773 362
Cuts at: 2538 4031 4884 5351 5374 5709 5955 6967 7053
Size: 1493 853 467 23 335 246 1012 86
Cuts at: 7053 7065 7411 1
Size: 12 346 449
Fragments arranged by size:
1493 1012 853 773 539 467 449 394
362 346 335 246 239 133 86 72
25 23 12
BtgI C'CryG_G
Cuts at: 107 2216 3612 4769 107
Size: 2109 1396 1157 3197
Fragments arranged by size:
3197 2109 1396 1157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2923 5809 5990 6153 2923
Size: 2886 181 163 4629
Fragments arranged by size:
4629 2886 181 163
BtsI GCAGTG_nn'
Cuts at: 153 1987 4224 4284 5102 153
Size: 1834 2237 60 818 2910
Fragments arranged by size:
2910 2237 1834 818 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4239 4274 4239
Size: 35 7824
DraI TTT'AAA
Cuts at: 2967 4858 2967
Size: 1891 5968
DraIII CAC_nnn'GTG
Cuts at: 4444 4444
Size: 7859
DrdI GACnn_nn'nnGTC
Cuts at: 1040 2416 5565 6423 1040
Size: 1376 3149 858 2476
Fragments arranged by size:
3149 2476 1376 858
EaeI y'GGCC_r
Cuts at: 851 2249 4154 5444 5618 6009 6036 7381 851
Size: 1398 1905 1290 174 391 27 1345 1329
Fragments arranged by size:
1905 1398 1345 1329 1290 391 174 27
EagI C'GGCC_G
Cuts at: 5444 5444
Size: 7859
EarI CTCTTCn'nnn_
Cuts at: 120 325 413 1067 1202 1614 1850 2032 3925
Size: 205 88 654 135 412 236 182 1893
Cuts at: 3925 4082 5882 6092 120
Size: 157 1800 210 1887
Fragments arranged by size:
1893 1887 1800 654 412 236 210 205
182 157 135 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6067 6377 6523 7669 6067
Size: 310 146 1146 6257
Fragments arranged by size:
6257 1146 310 146
EcoICRI GAG'CTC
Cuts at: 318 4332 4871 318
Size: 4014 539 3306
Fragments arranged by size:
4014 3306 539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 626 650 685 1438 1595 1969 2416 2695 3491
Size: 24 35 753 157 374 447 279 796
Cuts at: 3491 3601 3963 4126 4418 4469 5684 6116 6863
Size: 110 362 163 292 51 1215 432 747
Cuts at: 6863 7131 626
Size: 268 1354
Fragments arranged by size:
1354 1215 796 753 747 447 432 374
362 292 279 268 163 157 110 51
35 24
EcoNI CCTnn'n_nnAGG
Cuts at: 4424 4424
Size: 7859
EcoO109I rG'GnC_Cy
Cuts at: 1042 4480 4757 4998 1042
Size: 3438 277 241 3903
Fragments arranged by size:
3903 3438 277 241
EcoRI G'AATT_C
Cuts at: 4863 4863
Size: 7859
EcoRV GAT'ATC
Cuts at: 1584 1584
Size: 7859
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1081 1253 2371 2392 3252 5878 1081
Size: 172 1118 21 860 2626 3062
Fragments arranged by size:
3062 2626 1118 860 172 21
FspI TGC'GCA
Cuts at: 5640 5640
Size: 7859
HaeII r_GCGC'y
Cuts at: 4791 5293 5541 6563 7542 4791
Size: 502 248 1022 979 5108
Fragments arranged by size:
5108 1022 979 502 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1201 1233 1568 1600 2257 2289 2317 2349 2395
Size: 32 335 32 657 32 28 32 46
Cuts at: 2395 2427 2981 3013 3817 3849 4523 4555 4682
Size: 32 554 32 804 32 674 32 127
Cuts at: 4682 4714 5701 5731 5733 5763 1201
Size: 32 987 30 2 30 3297
Fragments arranged by size:
3297 987 804 674 657 554 335 127
46 32 32 32 32 32 32 32
32 32 30 30 28 2
HincII GTy'rAC
Cuts at: 188 4898 188
Size: 4710 3149
KasI G'GCGC_C
Cuts at: 4787 5289 5537 4787
Size: 502 248 7109
Fragments arranged by size:
7109 502 248
KpnI G_GTAC'C
Cuts at: 4879 4879
Size: 7859
MfeI C'AATT_G
Cuts at: 3989 3989
Size: 7859
MluI A'CGCG_T
Cuts at: 7292 7292
Size: 7859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 256 423 1040 1276 2967 4673 6530 6714 256
Size: 167 617 236 1691 1706 1857 184 1401
Fragments arranged by size:
1857 1706 1691 1401 617 236 184 167
MscI TGG'CCA
Cuts at: 853 2251 4156 5620 853
Size: 1398 1905 1464 3092
Fragments arranged by size:
3092 1905 1464 1398
MslI CAynn'nnrTG
Cuts at: 1907 2221 2486 2807 3566 3730 4768 5848 5975
Size: 314 265 321 759 164 1038 1080 127
Cuts at: 5975 1907
Size: 3791
Fragments arranged by size:
3791 1080 1038 759 321 314 265 164
127
MspA1I CmG'CkG
Cuts at: 155 3070 4528 4593 4970 5284 5644 6657 6902
Size: 2915 1458 65 377 314 360 1013 245
Cuts at: 6902 155
Size: 1112
Fragments arranged by size:
2915 1458 1112 1013 377 360 314 245
65
NaeI GCC'GGC
Cuts at: 6040 6040
Size: 7859
NarI GG'CG_CC
Cuts at: 4788 5290 5538 4788
Size: 502 248 7109
Fragments arranged by size:
7109 502 248
NcoI C'CATG_G
Cuts at: 107 2216 3612 4769 107
Size: 2109 1396 1157 3197
Fragments arranged by size:
3197 2109 1396 1157
NdeI CA'TA_TG
Cuts at: 2636 4455 2636
Size: 1819 6040
NgoMIV G'CCGG_C
Cuts at: 6038 6038
Size: 7859
NspI r_CATG'y
Cuts at: 2979 3576 3791 4912 5412 6319 2979
Size: 597 215 1121 500 907 4519
Fragments arranged by size:
4519 1121 907 597 500 215
PciI A'CATG_T
Cuts at: 3572 3787 6315 3572
Size: 215 2528 5116
Fragments arranged by size:
5116 2528 215
PflMI CCAn_nnn'nTGG
Cuts at: 3618 4413 3618
Size: 795 7064
PfoI T'CCnGG_A
Cuts at: 662 1946 4808 662
Size: 1284 2862 3713
Fragments arranged by size:
3713 2862 1284
PmeI GTTT'AAAC
Cuts at: 4858 4858
Size: 7859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6998 7030 6998
Size: 32 7827
PpuMI rG'GwC_Cy
Cuts at: 1042 1042
Size: 7859
PsiI TTA'TAA
Cuts at: 488 893 1784 1931 488
Size: 405 891 147 6416
Fragments arranged by size:
6416 891 405 147
PspOMI G'GGCC_C
Cuts at: 1983 4757 1983
Size: 2774 5085
PstI C_TGCA'G
Cuts at: 1029 2922 3015 3841 4906 5591 1029
Size: 1893 93 826 1065 685 3297
Fragments arranged by size:
3297 1893 1065 826 685 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7859
PvuII CAG'CTG
Cuts at: 3070 4528 5284 5644 3070
Size: 1458 756 360 5285
Fragments arranged by size:
5285 1458 756 360
RsrII CG'GwC_CG
Cuts at: 6054 6054
Size: 7859
SacI G_AGCT'C
Cuts at: 320 4334 4873 320
Size: 4014 539 3306
Fragments arranged by size:
4014 3306 539
SalI G'TCGA_C
Cuts at: 4896 4896
Size: 7859
SapI GCTCTTCn'nnn_
Cuts at: 120 325 5882 6092 120
Size: 205 5557 210 1887
Fragments arranged by size:
5557 1887 210 205
SbfI CC_TGCA'GG
Cuts at: 3841 4906 3841
Size: 1065 6794
ScaI AGT'ACT
Cuts at: 7376 7376
Size: 7859
SexAI A'CCwGG_T
Cuts at: 3534 4440 3534
Size: 906 6953
SfcI C'TryA_G
Cuts at: 33 236 788 1025 2505 2918 2958 3011 3837
Size: 203 552 237 1480 413 40 53 826
Cuts at: 3837 4902 5587 6580 6771 33
Size: 1065 685 993 191 1121
Fragments arranged by size:
1480 1121 1065 993 826 685 552 413
237 203 191 53 40
SfoI GGC'GCC
Cuts at: 4789 5291 5539 4789
Size: 502 248 7109
Fragments arranged by size:
7109 502 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7859
SmaI CCC'GGG
Cuts at: 4881 4881
Size: 7859
SmlI C'TyrA_G
Cuts at: 796 908 1706 1960 2023 4366 5014 5929 6421
Size: 112 798 254 63 2343 648 915 492
Cuts at: 6421 6683 796
Size: 262 1972
Fragments arranged by size:
2343 1972 915 798 648 492 262 254
112 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7859
SphI G_CATG'C
Cuts at: 4912 5412 4912
Size: 500 7359
SspI AAT'ATT
Cuts at: 944 944
Size: 7859
StuI AGG'CCT
Cuts at: 2924 4612 2924
Size: 1688 6171
StyI C'CwwG_G
Cuts at: 107 1065 1305 2216 3612 4407 4769 4993 5347
Size: 958 240 911 1396 795 362 224 354
Cuts at: 5347 107
Size: 2619
Fragments arranged by size:
2619 1396 958 911 795 362 354 240
224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3716 4325 4575 5442 5575 6034 6243 3716
Size: 609 250 867 133 459 209 5332
Fragments arranged by size:
5332 867 609 459 250 209 133
TatI w'GTAC_w
Cuts at: 1116 2314 7374 1116
Size: 1198 5060 1601
Fragments arranged by size:
5060 1601 1198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4451 4483 7376 7408 4451
Size: 32 2893 32 4902
Fragments arranged by size:
4902 2893 32 32
Tth111I GACn'n_nGTC
Cuts at: 2290 3595 5656 2290
Size: 1305 2061 4493
Fragments arranged by size:
4493 2061 1305
XbaI T'CTAG_A
Cuts at: 2173 3144 4890 2173
Size: 971 1746 5142
Fragments arranged by size:
5142 1746 971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5354 5354
Size: 7859
XhoI C'TCGA_G
Cuts at: 1706 1706
Size: 7859
XmaI C'CCGG_G
Cuts at: 4879 4879
Size: 7859
XmnI GAAnn'nnTTC
Cuts at: 728 2231 3752 3916 728
Size: 1503 1521 164 4671
Fragments arranged by size:
4671 1521 1503 164
ZraI GAC'GTC
Cuts at: 712 4698 712
Size: 3986 3873
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BssHII BstEII
BstZ17I Bsu36I ClaI FseI FspAI HindIII HpaI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI