Restriction map for
pF1KE9255
(Circular) (Six-base) MAPSORT of: pF1KE9255.seq Check: 2106 from: 1 to: 6704
pF1KE9255 6704 bp
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 414 414
Size: 6704
AccI GT'mk_AC
Cuts at: 990 1769 3742 990
Size: 779 1973 3952
Fragments arranged by size:
3952 1973 779
Acc65I G'GTAC_C
Cuts at: 3720 3720
Size: 6704
AclI AA'CG_TT
Cuts at: 1913 3569 6544 1913
Size: 1656 2975 2073
Fragments arranged by size:
2975 2073 1656
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 854 880 1238 1433 1939 2297 2900 2998 3110
Size: 26 358 195 506 358 603 98 112
Cuts at: 3110 4529 4961 5708 5976 854
Size: 1419 432 747 268 1582
Fragments arranged by size:
1582 1419 747 603 506 432 358 358
268 195 112 98 26
AflIII A'CryG_T
Cuts at: 3687 4056 5160 6137 3687
Size: 369 1104 977 4254
Fragments arranged by size:
4254 1104 977 369
AgeI A'CCGG_T
Cuts at: 5092 5092
Size: 6704
AhdI GACnn_n'nnGTC
Cuts at: 3231 3231
Size: 6704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1265 1265
Size: 6704
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 380 412 380
Size: 32 6672
AlwNI CAG_nnn'CTG
Cuts at: 2461 3088 3498 4009 5576 2461
Size: 627 410 511 1567 3589
Fragments arranged by size:
3589 1567 627 511 410
ApaLI G'TGCA_C
Cuts at: 5474 5474
Size: 6704
ApoI r'AATT_y
Cuts at: 1847 3149 3708 6057 6313 1847
Size: 1302 559 2349 256 2238
Fragments arranged by size:
2349 2238 1302 559 256
AseI AT'TA_AT
Cuts at: 20 2930 3397 20
Size: 2910 467 3327
Fragments arranged by size:
3327 2910 467
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6704
AvaI C'yCGr_G
Cuts at: 3724 3724
Size: 6704
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 830 863 830
Size: 33 6671
BamHI G'GATC_C
Cuts at: 1641 3729 1641
Size: 2088 4616
BanI G'GyrC_C
Cuts at: 3720 4134 4382 4417 3720
Size: 414 248 35 6007
Fragments arranged by size:
6007 414 248 35
BanII G_rGCy'C
Cuts at: 2199 2594 3718 4748 2199
Size: 395 1124 1030 4155
Fragments arranged by size:
4155 1124 1030 395
BbeI G_GCGC'C
Cuts at: 4138 4386 4138
Size: 248 6456
BbsI GAAGACnn'nnnn_
Cuts at: 3173 3173
Size: 6704
BbvCI CC'TCA_GC
Cuts at: 857 2684 857
Size: 1827 4877
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1546 1580 90
Size: 34 1422 34 5214
Fragments arranged by size:
5214 1422 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2910 4602 5363 6704 2910
Size: 1692 761 1341 2910
Fragments arranged by size:
2910 1692 1341 761
BclI T'GATC_A
Cuts at: 4224 4224
Size: 6704
BfrBI ATG'CAT
Cuts at: 111 2527 111
Size: 2416 4288
BglI GCCn_nnn'nGGC
Cuts at: 1360 1360
Size: 6704
BglII A'GATC_T
Cuts at: 4219 6256 4219
Size: 2037 4667
BlpI GC'TnA_GC
Cuts at: 677 852 1025 3816 677
Size: 175 173 2791 3565
Fragments arranged by size:
3565 2791 175 173
Bme1580I G_kGCm'C
Cuts at: 4329 4422 5478 4329
Size: 93 1056 5555
Fragments arranged by size:
5555 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3916 3997 4332 6476 3916
Size: 81 335 2144 4144
Fragments arranged by size:
4144 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1768 1904 5071 1768
Size: 136 3167 3401
Fragments arranged by size:
3401 3167 136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 1004 1763 2290 3331 566
Size: 438 759 527 1041 3939
Fragments arranged by size:
3939 1041 759 527 438
Bpu10I CC'TnA_GC
Cuts at: 857 2684 857
Size: 1827 4877
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 2578 2705 3880 4759 5251 5549 1048
Size: 1530 127 1175 879 492 298 2203
Fragments arranged by size:
2203 1530 1175 879 492 298 127
BsaI GGTCTCn'nnnn_
Cuts at: 6408 6408
Size: 6704
BsaAI yAC'GTr
Cuts at: 4057 4057
Size: 6704
BsaBI GATnn'nnATC
Cuts at: 6 4218 6255 6
Size: 4212 2037 455
Fragments arranged by size:
4212 2037 455
BsaHI Gr'CG_yC
Cuts at: 4135 4383 5085 6581 4135
Size: 248 702 1496 4258
Fragments arranged by size:
4258 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3893 4414 5092 5366 5513 5924 3893
Size: 521 678 274 147 411 4673
Fragments arranged by size:
4673 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2057 2087 3674 3704 2057
Size: 30 1587 30 5057
Fragments arranged by size:
5057 1587 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2389 2389
Size: 6704
BseYI C'CCAG_C
Cuts at: 252 4129 4887 5464 5960 252
Size: 3877 758 577 496 996
Fragments arranged by size:
3877 996 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 632 1217 632
Size: 585 6119
BsiEI CG_ry'CG
Cuts at: 104 4246 4292 5500 104
Size: 4142 46 1208 1308
Fragments arranged by size:
4142 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2109 2594 3687 3718 4496 4686 5478 2109
Size: 485 1093 31 778 190 792 3335
Fragments arranged by size:
3335 1093 792 778 485 190 31
Bsp1286I G_dGCh'C
Cuts at: 2109 2199 2594 3687 3718 4329 4422 4496 4686
Size: 90 395 1093 31 611 93 74 190
Cuts at: 4686 4748 5478 2109
Size: 62 730 3335
Fragments arranged by size:
3335 1093 730 611 395 190 93 90
74 62 31
BspEI T'CCGG_A
Cuts at: 5924 5924
Size: 6704
BspHI T'CATG_A
Cuts at: 399 942 5880 399
Size: 543 4938 1223
Fragments arranged by size:
4938 1223 543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 414 2206 3432 3754 3968 4270 4651 414
Size: 1792 1226 322 214 302 381 2467
Fragments arranged by size:
2467 1792 1226 381 322 302 214
BsrBI CCG'CTC
Cuts at: 50 4996 5050 6534 6699 50
Size: 4946 54 1484 165 55
Fragments arranged by size:
4946 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1970 4616 1970
Size: 2646 4058
BsrFI r'CCGG_y
Cuts at: 4702 4883 5092 4702
Size: 181 209 6314
Fragments arranged by size:
6314 209 181
BssSI C'ACGA_G
Cuts at: 1565 4975 5333 1565
Size: 3410 358 2936
Fragments arranged by size:
3410 2936 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1300 4081 1300
Size: 2781 3923
BstBI TT'CG_AA
Cuts at: 5065 5065
Size: 6704
BstEII G'GTnAC_C
Cuts at: 2730 2730
Size: 6704
BstXI CCAn_nnnn'nTGG
Cuts at: 231 2022 3487 3591 231
Size: 1791 1465 104 3344
Fragments arranged by size:
3344 1791 1465 104
BstYI r'GATC_y
Cuts at: 1 492 1641 3465 3729 4196 4219 4554 4800
Size: 491 1149 1824 264 467 23 335 246
Cuts at: 4800 5812 5898 5910 6256 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1824 1149 1012 491 467 449 346 335
264 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 991 991
Size: 6704
Bsu36I CC'TnA_GG
Cuts at: 3327 3327
Size: 6704
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4654 4835 4998 4654
Size: 181 163 6360
Fragments arranged by size:
6360 181 163
BtsI GCAGTG_nn'
Cuts at: 2228 2266 3947 2228
Size: 38 1681 4985
Fragments arranged by size:
4985 1681 38
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 407 442 407
Size: 35 6669
DraI TTT'AAA
Cuts at: 3703 3703
Size: 6704
DrdI GACnn_nn'nnGTC
Cuts at: 4410 5268 4410
Size: 858 5846
EaeI y'GGCC_r
Cuts at: 2952 4289 4463 4854 4881 6226 2952
Size: 1337 174 391 27 1345 3430
Fragments arranged by size:
3430 1345 1337 391 174 27
EagI C'GGCC_G
Cuts at: 4289 4289
Size: 6704
EarI CTCTTCn'nnn_
Cuts at: 190 502 704 2198 2273 2582 2720 3086 3115
Size: 312 202 1494 75 309 138 366 29
Cuts at: 3115 3421 4727 4937 190
Size: 306 1306 210 1957
Fragments arranged by size:
1957 1494 1306 366 312 309 306 210
202 138 75 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4912 5222 5368 6514 4912
Size: 310 146 1146 5102
Fragments arranged by size:
5102 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2592 3716 2592
Size: 1124 5580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 854 880 1004 1238 1433 1763 1939 2290
Size: 288 26 124 234 195 330 176 351
Cuts at: 2290 2297 2900 2998 3110 3331 4529 4961 5708
Size: 7 603 98 112 221 1198 432 747
Cuts at: 5708 5976 566
Size: 268 1294
Fragments arranged by size:
1294 1198 747 603 432 351 330 288
268 234 221 195 176 124 112 98
26 7
EcoNI CCTnn'n_nnAGG
Cuts at: 271 3445 271
Size: 3174 3530
EcoO109I rG'GnC_Cy
Cuts at: 3843 3843
Size: 6704
EcoRI G'AATT_C
Cuts at: 3708 3708
Size: 6704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1030 1315 1504 3342 4723 1030
Size: 285 189 1838 1381 3011
Fragments arranged by size:
3011 1838 1381 285 189
FspI TGC'GCA
Cuts at: 4485 4485
Size: 6704
HaeII r_GCGC'y
Cuts at: 4138 4386 5408 6387 4138
Size: 248 1022 979 4455
Fragments arranged by size:
4455 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 577 609 733 765 2593 2625 3217 3249 4546
Size: 32 124 32 1828 32 592 32 1297
Cuts at: 4546 4576 4578 4608 577
Size: 30 2 30 2673
Fragments arranged by size:
2673 1828 1297 592 124 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 522 3743 522
Size: 3221 3483
HindIII A'AGCT_T
Cuts at: 892 892
Size: 6704
HpaI GTT'AAC
Cuts at: 522 522
Size: 6704
KasI G'GCGC_C
Cuts at: 4134 4382 4134
Size: 248 6456
KpnI G_GTAC'C
Cuts at: 3724 3724
Size: 6704
MluI A'CGCG_T
Cuts at: 6137 6137
Size: 6704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 287 745 5375 5559 248
Size: 39 458 4630 184 1393
Fragments arranged by size:
4630 1393 458 184 39
MscI TGG'CCA
Cuts at: 2954 4465 2954
Size: 1511 5193
MslI CAynn'nnrTG
Cuts at: 1247 3532 4693 4820 1247
Size: 2285 1161 127 3131
Fragments arranged by size:
3131 2285 1161 127
MspA1I CmG'CkG
Cuts at: 613 856 1231 1261 1560 2683 3815 4129 4489
Size: 243 375 30 299 1123 1132 314 360
Cuts at: 4489 5502 5747 613
Size: 1013 245 1570
Fragments arranged by size:
1570 1132 1123 1013 375 360 314 299
245 243 30
NaeI GCC'GGC
Cuts at: 4885 4885
Size: 6704
NarI GG'CG_CC
Cuts at: 4135 4383 4135
Size: 248 6456
NdeI CA'TA_TG
Cuts at: 1593 1593
Size: 6704
NgoMIV G'CCGG_C
Cuts at: 4883 4883
Size: 6704
NsiI A_TGCA'T
Cuts at: 113 2529 113
Size: 2416 4288
NspI r_CATG'y
Cuts at: 1085 1189 1384 3691 3757 4257 5164 1085
Size: 104 195 2307 66 500 907 2625
Fragments arranged by size:
2625 2307 907 500 195 104 66
PciI A'CATG_T
Cuts at: 3687 5160 3687
Size: 1473 5231
PmeI GTTT'AAAC
Cuts at: 3703 3703
Size: 6704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2043 2075 5843 5875 2043
Size: 32 3768 32 2872
Fragments arranged by size:
3768 2872 32 32
PshAI GACnn'nnGTC
Cuts at: 163 163
Size: 6704
PsiI TTA'TAA
Cuts at: 889 889
Size: 6704
PstI C_TGCA'G
Cuts at: 3751 4436 3751
Size: 685 6019
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6704
PvuII CAG'CTG
Cuts at: 613 856 1231 1560 2683 4129 4489 613
Size: 243 375 329 1123 1446 360 2828
Fragments arranged by size:
2828 1446 1123 375 360 329 243
RsrII CG'GwC_CG
Cuts at: 4899 4899
Size: 6704
SacI G_AGCT'C
Cuts at: 2594 3718 2594
Size: 1124 5580
SalI G'TCGA_C
Cuts at: 3741 3741
Size: 6704
SapI GCTCTTCn'nnn_
Cuts at: 2582 3086 4727 4937 2582
Size: 504 1641 210 4349
Fragments arranged by size:
4349 1641 504 210
SbfI CC_TGCA'GG
Cuts at: 3751 3751
Size: 6704
ScaI AGT'ACT
Cuts at: 6221 6221
Size: 6704
SfcI C'TryA_G
Cuts at: 33 659 1437 3161 3605 3747 4432 5425 5616
Size: 626 778 1724 444 142 685 993 191
Cuts at: 5616 33
Size: 1121
Fragments arranged by size:
1724 1121 993 778 685 626 444 191
142
SfoI GGC'GCC
Cuts at: 4136 4384 4136
Size: 248 6456
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6704
SmaI CCC'GGG
Cuts at: 3726 3726
Size: 6704
SmlI C'TyrA_G
Cuts at: 1063 2593 2720 3859 4774 5266 5528 1063
Size: 1530 127 1139 915 492 262 2239
Fragments arranged by size:
2239 1530 1139 915 492 262 127
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6704
SphI G_CATG'C
Cuts at: 1189 1384 3757 4257 1189
Size: 195 2373 500 3636
Fragments arranged by size:
3636 2373 500 195
SspI AAT'ATT
Cuts at: 451 3201 451
Size: 2750 3954
StyI C'CwwG_G
Cuts at: 1931 3461 3662 3838 4192 1931
Size: 1530 201 176 354 4443
Fragments arranged by size:
4443 1530 354 201 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 175 3690 4287 4420 4879 5088 175
Size: 3515 597 133 459 209 1791
Fragments arranged by size:
3515 1791 597 459 209 133
TatI w'GTAC_w
Cuts at: 486 6219 486
Size: 5733 971
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1818 1850 2057 2082 2089 2114 3674 3706 6221
Size: 32 207 25 7 25 1560 32 2515
Cuts at: 6221 6253 1818
Size: 32 2269
Fragments arranged by size:
2515 2269 1560 207 32 32 32 25
25 7
Tth111I GACn'n_nGTC
Cuts at: 4501 4501
Size: 6704
XbaI T'CTAG_A
Cuts at: 129 1458 2199 3735 129
Size: 1329 741 1536 3098
Fragments arranged by size:
3098 1536 1329 741
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1652 4199 1652
Size: 2547 4157
XmaI C'CCGG_G
Cuts at: 3724 3724
Size: 6704
XmnI GAAnn'nnTTC
Cuts at: 3642 3642
Size: 6704
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PciI PmeI PpiI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI ApaI AscI AvrII BmgBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BtgI ClaI
DraIII EcoRV FseI FspAI MfeI NcoI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI