Restriction map for
pF1KE8000
(Circular) (Six-base) MAPSORT of: pF1KE8000.seq Check: 843 from: 1 to: 4415
pF1KE8000 4415 bp
With 182 enzymes: SgfI *
February 29, 2012 11:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1033 1033
Size: 4415
AccI GT'mk_AC
Cuts at: 1453 1453
Size: 4415
Acc65I G'GTAC_C
Cuts at: 1431 1431
Size: 4415
AclI AA'CG_TT
Cuts at: 4255 4255
Size: 4415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 882 955 1117 1246 1300 2240 2672 3419 3687
Size: 73 162 129 54 940 432 747 268
Cuts at: 3687 882
Size: 1610
Fragments arranged by size:
1610 940 747 432 268 162 129 73
54
AflIII A'CryG_T
Cuts at: 277 842 854 1767 2871 3848 277
Size: 565 12 913 1104 977 844
Fragments arranged by size:
1104 977 913 844 565 12
AgeI A'CCGG_T
Cuts at: 2803 2803
Size: 4415
AhdI GACnn_n'nnGTC
Cuts at: 168 168
Size: 4415
AlwNI CAG_nnn'CTG
Cuts at: 172 519 534 650 773 1338 1720 3287 172
Size: 347 15 116 123 565 382 1567 1300
Fragments arranged by size:
1567 1300 565 382 347 123 116 15
ApaLI G'TGCA_C
Cuts at: 3185 3185
Size: 4415
ApoI r'AATT_y
Cuts at: 618 1081 1419 3768 4024 618
Size: 463 338 2349 256 1009
Fragments arranged by size:
2349 1009 463 338 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4415
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
AvaI C'yCGr_G
Cuts at: 1435 1435
Size: 4415
BamHI G'GATC_C
Cuts at: 1440 1440
Size: 4415
BanI G'GyrC_C
Cuts at: 335 352 1431 1845 2093 2128 335
Size: 17 1079 414 248 35 2622
Fragments arranged by size:
2622 1079 414 248 35 17
BanII G_rGCy'C
Cuts at: 1073 1307 1429 2459 1073
Size: 234 122 1030 3029
Fragments arranged by size:
3029 1030 234 122
BbeI G_GCGC'C
Cuts at: 356 1849 2097 356
Size: 1493 248 2674
Fragments arranged by size:
2674 1493 248
BbsI GAAGACnn'nnnn_
Cuts at: 379 1040 379
Size: 661 3754
BbvCI CC'TCA_GC
Cuts at: 331 331
Size: 4415
BciVI GTATCCnnnnn_n'
Cuts at: 2313 3074 4415 2313
Size: 761 1341 2313
Fragments arranged by size:
2313 1341 761
BclI T'GATC_A
Cuts at: 440 479 557 1935 440
Size: 39 78 1378 2920
Fragments arranged by size:
2920 1378 78 39
BglII A'GATC_T
Cuts at: 720 812 923 1930 3967 720
Size: 92 111 1007 2037 1168
Fragments arranged by size:
2037 1168 1007 111 92
BlpI GC'TnA_GC
Cuts at: 640 1322 1527 640
Size: 682 205 3528
Fragments arranged by size:
3528 682 205
Bme1580I G_kGCm'C
Cuts at: 340 2040 2133 3189 340
Size: 1700 93 1056 1566
Fragments arranged by size:
1700 1566 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 182 529 1627 1708 2043 4187 182
Size: 347 1098 81 335 2144 410
Fragments arranged by size:
2144 1098 410 347 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 834 2782 834
Size: 1948 2467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1087 436
Size: 651 3764
Bpu10I CC'TnA_GC
Cuts at: 331 331
Size: 4415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1591 2470 2962 3260 1591
Size: 879 492 298 2746
Fragments arranged by size:
2746 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 111 744 4119 111
Size: 633 3375 407
Fragments arranged by size:
3375 633 407
BsaAI yAC'GTr
Cuts at: 1768 1768
Size: 4415
BsaBI GATnn'nnATC
Cuts at: 6 556 1929 3966 6
Size: 550 1373 2037 455
Fragments arranged by size:
2037 1373 550 455
BsaHI Gr'CG_yC
Cuts at: 353 1846 2094 2796 4292 353
Size: 1493 248 702 1496 476
Fragments arranged by size:
1496 1493 702 476 248
BsaWI w'CCGG_w
Cuts at: 344 713 1604 2125 2803 3077 3224 3635 344
Size: 369 891 521 678 274 147 411 1124
Fragments arranged by size:
1124 891 678 521 411 369 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 910 940 910
Size: 30 4385
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 478 556 1132 1135 478
Size: 78 576 3 3758
Fragments arranged by size:
3758 576 78 3
BseYI C'CCAG_C
Cuts at: 789 1253 1331 1840 2598 3175 3671 789
Size: 464 78 509 758 577 496 1533
Fragments arranged by size:
1533 758 577 509 496 464 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 547
Size: 4415
BsiEI CG_ry'CG
Cuts at: 104 1957 2003 3211 104
Size: 1853 46 1208 1308
Fragments arranged by size:
1853 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 730 1307 1429 2207 2397 3189 730
Size: 577 122 778 190 792 1956
Fragments arranged by size:
1956 792 778 577 190 122
BsmI GAATG_Cn'
Cuts at: 1246 1246
Size: 4415
Bsp1286I G_dGCh'C
Cuts at: 340 730 1073 1307 1429 2040 2133 2207 2397
Size: 390 343 234 122 611 93 74 190
Cuts at: 2397 2459 3189 340
Size: 62 730 1566
Fragments arranged by size:
1566 730 611 390 343 234 190 122
93 74 62
BspEI T'CCGG_A
Cuts at: 344 713 3635 344
Size: 369 2922 1124
Fragments arranged by size:
2922 1124 369
BspHI T'CATG_A
Cuts at: 272 3591 272
Size: 3319 1096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1033 1465 1679 1981 2362 1033
Size: 432 214 302 381 3086
Fragments arranged by size:
3086 432 381 302 214
BsrBI CCG'CTC
Cuts at: 50 422 2707 2761 4245 4410 50
Size: 372 2285 54 1484 165 55
Fragments arranged by size:
2285 1484 372 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2327 2327
Size: 4415
BsrFI r'CCGG_y
Cuts at: 1013 2413 2594 2803 1013
Size: 1400 181 209 2625
Fragments arranged by size:
2625 1400 209 181
BsrGI T'GTAC_A
Cuts at: 150 150
Size: 4415
BssSI C'ACGA_G
Cuts at: 2686 3044 2686
Size: 358 4057
BstAPI GCAn_nnn'nTGC
Cuts at: 650 1792 650
Size: 1142 3273
BstBI TT'CG_AA
Cuts at: 2776 2776
Size: 4415
BstYI r'GATC_y
Cuts at: 1 720 812 923 1440 1907 1930 2265 2511
Size: 719 92 111 517 467 23 335 246
Cuts at: 2511 3523 3609 3621 3967 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 719 517 467 449 346 335 246
111 92 86 23 12
BtgI C'CryG_G
Cuts at: 107 458 107
Size: 351 4064
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2365 2546 2709 2365
Size: 181 163 4071
Fragments arranged by size:
4071 181 163
BtsI GCAGTG_nn'
Cuts at: 651 774 1037 1658 651
Size: 123 263 621 3408
Fragments arranged by size:
3408 621 263 123
DraI TTT'AAA
Cuts at: 1414 1414
Size: 4415
DraIII CAC_nnn'GTG
Cuts at: 1188 1188
Size: 4415
DrdI GACnn_nn'nnGTC
Cuts at: 2121 2979 2121
Size: 858 3557
EaeI y'GGCC_r
Cuts at: 1011 2000 2174 2565 2592 3937 1011
Size: 989 174 391 27 1345 1489
Fragments arranged by size:
1489 1345 989 391 174 27
EagI C'GGCC_G
Cuts at: 2000 2000
Size: 4415
EarI CTCTTCn'nnn_
Cuts at: 1156 2438 2648 1156
Size: 1282 210 2923
Fragments arranged by size:
2923 1282 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2623 2933 3079 4225 2623
Size: 310 146 1146 2813
Fragments arranged by size:
2813 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1305 1427 1305
Size: 122 4293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 882 955 1087 1117 1246 1300 2240 2672
Size: 446 73 132 30 129 54 940 432
Cuts at: 2672 3419 3687 436
Size: 747 268 1164
Fragments arranged by size:
1164 940 747 446 432 268 132 129
73 54 30
EcoNI CCTnn'n_nnAGG
Cuts at: 897 934 1381 897
Size: 37 447 3931
Fragments arranged by size:
3931 447 37
EcoO109I rG'GnC_Cy
Cuts at: 217 675 1554 217
Size: 458 879 3078
Fragments arranged by size:
3078 879 458
EcoRI G'AATT_C
Cuts at: 1419 1419
Size: 4415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4415
FspI TGC'GCA
Cuts at: 406 1345 2196 406
Size: 939 851 2625
Fragments arranged by size:
2625 939 851
HaeII r_GCGC'y
Cuts at: 356 1849 2097 3119 4098 356
Size: 1493 248 1022 979 673
Fragments arranged by size:
1493 1022 979 673 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 495 527 561 593 712 744 910
Size: 32 309 32 34 32 119 32 166
Cuts at: 910 942 2257 2287 2289 2319 154
Size: 32 1315 30 2 30 2250
Fragments arranged by size:
2250 1315 309 166 119 34 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 195 848 961 1454 195
Size: 653 113 493 3156
Fragments arranged by size:
3156 653 493 113
KasI G'GCGC_C
Cuts at: 352 1845 2093 352
Size: 1493 248 2674
Fragments arranged by size:
2674 1493 248
KpnI G_GTAC'C
Cuts at: 1435 1435
Size: 4415
MluI A'CGCG_T
Cuts at: 3848 3848
Size: 4415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 192 473 3086 3270 192
Size: 281 2613 184 1337
Fragments arranged by size:
2613 1337 281 184
MscI TGG'CCA
Cuts at: 2176 2176
Size: 4415
MslI CAynn'nnrTG
Cuts at: 112 266 1291 2404 2531 112
Size: 154 1025 1113 127 1996
Fragments arranged by size:
1996 1113 1025 154 127
MspA1I CmG'CkG
Cuts at: 549 750 1110 1200 1335 1526 1840 2200 3213
Size: 201 360 90 135 191 314 360 1013
Cuts at: 3213 3458 549
Size: 245 1506
Fragments arranged by size:
1506 1013 360 360 314 245 201 191
135 90
NaeI GCC'GGC
Cuts at: 1015 2596 1015
Size: 1581 2834
NarI GG'CG_CC
Cuts at: 353 1846 2094 353
Size: 1493 248 2674
Fragments arranged by size:
2674 1493 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4415
NgoMIV G'CCGG_C
Cuts at: 1013 2594 1013
Size: 1581 2834
NspI r_CATG'y
Cuts at: 281 846 858 1095 1468 1968 2875 281
Size: 565 12 237 373 500 907 1821
Fragments arranged by size:
1821 907 565 500 373 237 12
PciI A'CATG_T
Cuts at: 277 842 854 2871 277
Size: 565 12 2017 1821
Fragments arranged by size:
2017 1821 565 12
PfoI T'CCnGG_A
Cuts at: 177 1349 177
Size: 1172 3243
PmeI GTTT'AAAC
Cuts at: 1414 1414
Size: 4415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4383
PpuMI rG'GwC_Cy
Cuts at: 217 217
Size: 4415
PstI C_TGCA'G
Cuts at: 674 749 1044 1109 1181 1205 1462 2147 674
Size: 75 295 65 72 24 257 685 2942
Fragments arranged by size:
2942 685 295 257 75 72 65 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4415
PvuII CAG'CTG
Cuts at: 750 1110 1200 1840 2200 750
Size: 360 90 640 360 2965
Fragments arranged by size:
2965 640 360 360 90
RsrII CG'GwC_CG
Cuts at: 2610 2610
Size: 4415
SacI G_AGCT'C
Cuts at: 1307 1429 1307
Size: 122 4293
SalI G'TCGA_C
Cuts at: 1452 1452
Size: 4415
SapI GCTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
SbfI CC_TGCA'GG
Cuts at: 1462 1462
Size: 4415
ScaI AGT'ACT
Cuts at: 3932 3932
Size: 4415
SfcI C'TryA_G
Cuts at: 33 670 745 1040 1105 1177 1201 1458 2143
Size: 637 75 295 65 72 24 257 685
Cuts at: 2143 3136 3327 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 637 295 257 191 75
72 65 24
SfoI GGC'GCC
Cuts at: 354 1847 2095 354
Size: 1493 248 2674
Fragments arranged by size:
2674 1493 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
SmaI CCC'GGG
Cuts at: 1437 1437
Size: 4415
SmlI C'TyrA_G
Cuts at: 1570 2485 2977 3239 1570
Size: 915 492 262 2746
Fragments arranged by size:
2746 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4415
SphI G_CATG'C
Cuts at: 1468 1968 1468
Size: 500 3915
StuI AGG'CCT
Cuts at: 394 394
Size: 4415
StyI C'CwwG_G
Cuts at: 107 1549 1903 107
Size: 1442 354 2619
Fragments arranged by size:
2619 1442 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1345 1998 2131 2590 2799 1345
Size: 653 133 459 209 2961
Fragments arranged by size:
2961 653 459 209 133
TatI w'GTAC_w
Cuts at: 150 3930 150
Size: 3780 635
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 480 512 3932 3964 480
Size: 32 3420 32 931
Fragments arranged by size:
3420 931 32 32
Tth111I GACn'n_nGTC
Cuts at: 2212 2212
Size: 4415
XbaI T'CTAG_A
Cuts at: 1446 1446
Size: 4415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1910 1910
Size: 4415
XmaI C'CCGG_G
Cuts at: 1435 1435
Size: 4415
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI