Restriction map for
pF1KE6728
(Circular) (Six-base) MAPSORT of: pF1KE6728.seq Check: 6511 from: 1 to: 6038
pF1KE6728 6038 bp
With 182 enzymes: SgfI *
October 20, 2017 17:04 ..
AccI GT'mk_AC
Cuts at: 3076 3076
Size: 6038
Acc65I G'GTAC_C
Cuts at: 3054 3054
Size: 6038
AclI AA'CG_TT
Cuts at: 5878 5878
Size: 6038
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 1259 1355 2899 3863 4295 5042 5310 124
Size: 1135 96 1544 964 432 747 268 852
Fragments arranged by size:
1544 1135 964 852 747 432 268 96
AflII C'TTAA_G
Cuts at: 1641 1641
Size: 6038
AflIII A'CryG_T
Cuts at: 333 2085 3390 4494 5471 333
Size: 1752 1305 1104 977 900
Fragments arranged by size:
1752 1305 1104 977 900
AgeI A'CCGG_T
Cuts at: 4426 4426
Size: 6038
AleI CACnn'nnGTG
Cuts at: 1426 1426
Size: 6038
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1771 1803 2948 2980 1771
Size: 32 1145 32 4829
Fragments arranged by size:
4829 1145 32 32
AlwNI CAG_nnn'CTG
Cuts at: 855 1809 3343 4910 855
Size: 954 1534 1567 1983
Fragments arranged by size:
1983 1567 1534 954
ApaLI G'TGCA_C
Cuts at: 4808 4808
Size: 6038
ApoI r'AATT_y
Cuts at: 635 997 1119 1627 1747 2266 2554 2704 3042
Size: 362 122 508 120 519 288 150 338
Cuts at: 3042 5391 5647 635
Size: 2349 256 1026
Fragments arranged by size:
2349 1026 519 508 362 338 288 256
150 122 120
AseI AT'TA_AT
Cuts at: 20 20
Size: 6038
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6038
AvaI C'yCGr_G
Cuts at: 3058 3058
Size: 6038
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 161 194 161
Size: 33 6005
BamHI G'GATC_C
Cuts at: 3063 3063
Size: 6038
BanI G'GyrC_C
Cuts at: 3054 3468 3716 3751 3054
Size: 414 248 35 5341
Fragments arranged by size:
5341 414 248 35
BanII G_rGCy'C
Cuts at: 389 1499 3052 4082 389
Size: 1110 1553 1030 2345
Fragments arranged by size:
2345 1553 1110 1030
BbeI G_GCGC'C
Cuts at: 3472 3720 3472
Size: 248 5790
BbsI GAAGACnn'nnnn_
Cuts at: 1271 1271
Size: 6038
BbvCI CC'TCA_GC
Cuts at: 191 191
Size: 6038
BciVI GTATCCnnnnn_n'
Cuts at: 162 2468 2982 3936 4697 6038 162
Size: 2306 514 954 761 1341 162
Fragments arranged by size:
2306 1341 954 761 514 162
BclI T'GATC_A
Cuts at: 1518 3558 1518
Size: 2040 3998
BfrBI ATG'CAT
Cuts at: 1842 1842
Size: 6038
BglII A'GATC_T
Cuts at: 3553 5590 3553
Size: 2037 4001
BlpI GC'TnA_GC
Cuts at: 3150 3150
Size: 6038
Bme1580I G_kGCm'C
Cuts at: 3663 3756 4812 3663
Size: 93 1056 4889
Fragments arranged by size:
4889 1056 93
BmgBI CAC'GTC
Cuts at: 334 334
Size: 6038
BmrI ACTGGGnnnn_n'
Cuts at: 2041 3250 3331 3666 5810 2041
Size: 1209 81 335 2144 2269
Fragments arranged by size:
2269 2144 1209 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4405 4405
Size: 6038
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 348
Size: 6038
Bpu10I CC'TnA_GC
Cuts at: 191 191
Size: 6038
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2338 2827 2914 3214 4093 4585 4883 2338
Size: 489 87 300 879 492 298 3493
Fragments arranged by size:
3493 879 492 489 300 298 87
BsaI GGTCTCn'nnnn_
Cuts at: 5742 5742
Size: 6038
BsaAI yAC'GTr
Cuts at: 2477 3391 2477
Size: 914 5124
BsaBI GATnn'nnATC
Cuts at: 6 956 1517 3552 5589 6
Size: 950 561 2035 2037 455
Fragments arranged by size:
2037 2035 950 561 455
BsaHI Gr'CG_yC
Cuts at: 3469 3717 4419 5915 3469
Size: 248 702 1496 3592
Fragments arranged by size:
3592 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3227 3748 4426 4700 4847 5258 3227
Size: 521 678 274 147 411 4007
Fragments arranged by size:
4007 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1365 1395 1365
Size: 30 6008
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 373 376 965 1313 208
Size: 165 3 589 348 4933
Fragments arranged by size:
4933 589 348 165 3
BseYI C'CCAG_C
Cuts at: 2627 3005 3463 4221 4798 5294 2627
Size: 378 458 758 577 496 3371
Fragments arranged by size:
3371 758 577 496 458 378
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 401
Size: 6038
BsiEI CG_ry'CG
Cuts at: 104 3580 3626 4834 104
Size: 3476 46 1208 1308
Fragments arranged by size:
3476 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 443 1499 2354 3052 3830 4020 4812 443
Size: 1056 855 698 778 190 792 1669
Fragments arranged by size:
1669 1056 855 792 778 698 190
BsmI GAATG_Cn'
Cuts at: 865 1171 1446 865
Size: 306 275 5457
Fragments arranged by size:
5457 306 275
BsmBI CGTCTCn'nnnn_
Cuts at: 324 324
Size: 6038
Bsp1286I G_dGCh'C
Cuts at: 389 443 1499 2354 3052 3663 3756 3830 4020
Size: 54 1056 855 698 611 93 74 190
Cuts at: 4020 4082 4812 389
Size: 62 730 1615
Fragments arranged by size:
1615 1056 855 730 698 611 190 93
74 62 54
BspEI T'CCGG_A
Cuts at: 5258 5258
Size: 6038
BspHI T'CATG_A
Cuts at: 2750 5214 2750
Size: 2464 3574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3088 3302 3604 3985 3088
Size: 214 302 381 5141
Fragments arranged by size:
5141 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4330 4384 5868 6033 50
Size: 4280 54 1484 165 55
Fragments arranged by size:
4280 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 443 711 1579 3950 443
Size: 268 868 2371 2531
Fragments arranged by size:
2531 2371 868 268
BsrFI r'CCGG_y
Cuts at: 4036 4217 4426 4036
Size: 181 209 5648
Fragments arranged by size:
5648 209 181
BssSI C'ACGA_G
Cuts at: 4309 4667 4309
Size: 358 5680
BstAPI GCAn_nnn'nTGC
Cuts at: 346 454 1848 2946 3415 346
Size: 108 1394 1098 469 2969
Fragments arranged by size:
2969 1394 1098 469 108
BstBI TT'CG_AA
Cuts at: 4399 4399
Size: 6038
BstXI CCAn_nnnn'nTGG
Cuts at: 579 1428 579
Size: 849 5189
BstYI r'GATC_y
Cuts at: 1 111 2760 3063 3530 3553 3888 4134 5146
Size: 110 2649 303 467 23 335 246 1012
Cuts at: 5146 5232 5244 5590 1
Size: 86 12 346 449
Fragments arranged by size:
2649 1012 467 449 346 335 303 246
110 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 6038
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3988 4169 4332 3988
Size: 181 163 5694
Fragments arranged by size:
5694 181 163
BtsI GCAGTG_nn'
Cuts at: 214 443 739 2902 3281 214
Size: 229 296 2163 379 2971
Fragments arranged by size:
2971 2163 379 296 229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2463 2498 2463
Size: 35 6003
DraI TTT'AAA
Cuts at: 306 1530 2318 3037 306
Size: 1224 788 719 3307
Fragments arranged by size:
3307 1224 788 719
DrdI GACnn_nn'nnGTC
Cuts at: 3744 4602 3744
Size: 858 5180
EaeI y'GGCC_r
Cuts at: 3623 3797 4188 4215 5560 3623
Size: 174 391 27 1345 4101
Fragments arranged by size:
4101 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3623 3623
Size: 6038
EarI CTCTTCn'nnn_
Cuts at: 2675 4061 4271 2675
Size: 1386 210 4442
Fragments arranged by size:
4442 1386 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4246 4556 4702 5848 4246
Size: 310 146 1146 4436
Fragments arranged by size:
4436 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1497 3050 1497
Size: 1553 4485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 124 348 1259 1355 2899 3863 4295 5042 5310
Size: 224 911 96 1544 964 432 747 268
Cuts at: 5310 124
Size: 852
Fragments arranged by size:
1544 964 911 852 747 432 268 224
96
EcoNI CCTnn'n_nnAGG
Cuts at: 2768 2768
Size: 6038
EcoO109I rG'GnC_Cy
Cuts at: 1417 2883 3177 1417
Size: 1466 294 4278
Fragments arranged by size:
4278 1466 294
EcoRI G'AATT_C
Cuts at: 3042 3042
Size: 6038
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1656 4057 1656
Size: 2401 3637
FspI TGC'GCA
Cuts at: 3819 3819
Size: 6038
HaeII r_GCGC'y
Cuts at: 3472 3720 4742 5721 3472
Size: 248 1022 979 3789
Fragments arranged by size:
3789 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 411 443 1043 1075 1365 1397 2745 2777 2787
Size: 32 600 32 290 32 1348 32 10
Cuts at: 2787 2819 3880 3910 3912 3942 411
Size: 32 1061 30 2 30 2507
Fragments arranged by size:
2507 1348 1061 600 290 32 32 32
32 32 30 30 10 2
HincII GTy'rAC
Cuts at: 149 770 1730 1881 3077 149
Size: 621 960 151 1196 3110
Fragments arranged by size:
3110 1196 960 621 151
HpaI GTT'AAC
Cuts at: 149 770 1730 149
Size: 621 960 4457
Fragments arranged by size:
4457 960 621
KasI G'GCGC_C
Cuts at: 3468 3716 3468
Size: 248 5790
KpnI G_GTAC'C
Cuts at: 3058 3058
Size: 6038
MluI A'CGCG_T
Cuts at: 5471 5471
Size: 6038
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1458 4709 4893 1458
Size: 3251 184 2603
Fragments arranged by size:
3251 2603 184
MscI TGG'CCA
Cuts at: 3799 3799
Size: 6038
MslI CAynn'nnrTG
Cuts at: 1426 1837 4027 4154 1426
Size: 411 2190 127 3310
Fragments arranged by size:
3310 2190 411 127
MspA1I CmG'CkG
Cuts at: 190 3149 3463 3823 4836 5081 190
Size: 2959 314 360 1013 245 1147
Fragments arranged by size:
2959 1147 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4219 4219
Size: 6038
NarI GG'CG_CC
Cuts at: 3469 3717 3469
Size: 248 5790
NcoI C'CATG_G
Cuts at: 107 107
Size: 6038
NdeI CA'TA_TG
Cuts at: 1899 1899
Size: 6038
NgoMIV G'CCGG_C
Cuts at: 4217 4217
Size: 6038
NsiI A_TGCA'T
Cuts at: 1844 1844
Size: 6038
NspI r_CATG'y
Cuts at: 1846 1855 1887 2089 3091 3591 4498 1846
Size: 9 32 202 1002 500 907 3386
Fragments arranged by size:
3386 1002 907 500 202 32 9
PciI A'CATG_T
Cuts at: 2085 4494 2085
Size: 2409 3629
PflMI CCAn_nnn'nTGG
Cuts at: 267 479 2580 267
Size: 212 2101 3725
Fragments arranged by size:
3725 2101 212
PfoI T'CCnGG_A
Cuts at: 521 521
Size: 6038
PmeI GTTT'AAAC
Cuts at: 3037 3037
Size: 6038
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5177 5209 5177
Size: 32 6006
PpuMI rG'GwC_Cy
Cuts at: 1417 2883 1417
Size: 1466 4572
PsiI TTA'TAA
Cuts at: 641 1106 2719 2780 641
Size: 465 1613 61 3899
Fragments arranged by size:
3899 1613 465 61
PstI C_TGCA'G
Cuts at: 1814 2909 3085 3770 1814
Size: 1095 176 685 4082
Fragments arranged by size:
4082 1095 685 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6038
PvuII CAG'CTG
Cuts at: 190 3463 3823 190
Size: 3273 360 2405
Fragments arranged by size:
3273 2405 360
RsrII CG'GwC_CG
Cuts at: 4233 4233
Size: 6038
SacI G_AGCT'C
Cuts at: 1499 3052 1499
Size: 1553 4485
SalI G'TCGA_C
Cuts at: 3075 3075
Size: 6038
SapI GCTCTTCn'nnn_
Cuts at: 4061 4271 4061
Size: 210 5828
SbfI CC_TGCA'GG
Cuts at: 3085 3085
Size: 6038
ScaI AGT'ACT
Cuts at: 5555 5555
Size: 6038
SfcI C'TryA_G
Cuts at: 33 1810 2330 2462 2905 3010 3081 3766 4759
Size: 1777 520 132 443 105 71 685 993
Cuts at: 4759 4950 33
Size: 191 1121
Fragments arranged by size:
1777 1121 993 685 520 443 191 132
105 71
SfoI GGC'GCC
Cuts at: 3470 3718 3470
Size: 248 5790
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6038
SmaI CCC'GGG
Cuts at: 3060 3060
Size: 6038
SmlI C'TyrA_G
Cuts at: 1641 2353 2806 2929 3193 4108 4600 4862 1641
Size: 712 453 123 264 915 492 262 2817
Fragments arranged by size:
2817 915 712 492 453 264 262 123
SpeI A'CTAG_T
Cuts at: 67 2515 67
Size: 2448 3590
SphI G_CATG'C
Cuts at: 3091 3591 3091
Size: 500 5538
SspI AAT'ATT
Cuts at: 249 833 1921 249
Size: 584 1088 4366
Fragments arranged by size:
4366 1088 584
StuI AGG'CCT
Cuts at: 2738 2738
Size: 6038
StyI C'CwwG_G
Cuts at: 107 3172 3526 107
Size: 3065 354 2619
Fragments arranged by size:
3065 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1597 2856 3019 3621 3754 4213 4422 1597
Size: 1259 163 602 133 459 209 3213
Fragments arranged by size:
3213 1259 602 459 209 163 133
TatI w'GTAC_w
Cuts at: 294 1386 2062 5553 294
Size: 1092 676 3491 779
Fragments arranged by size:
3491 1092 779 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1929 1961 5555 5587 1929
Size: 32 3594 32 2380
Fragments arranged by size:
3594 2380 32 32
Tth111I GACn'n_nGTC
Cuts at: 3835 3835
Size: 6038
XbaI T'CTAG_A
Cuts at: 1392 3069 1392
Size: 1677 4361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 264 3533 264
Size: 3269 2769
XmaI C'CCGG_G
Cuts at: 3058 3058
Size: 6038
XmnI GAAnn'nnTTC
Cuts at: 250 491 2646 250
Size: 241 2155 3642
Fragments arranged by size:
3642 2155 241
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI ApaI AscI AvrII
BcgI BglI BmtI BsiWI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoRV FseI FspAI HindIII MfeI
NheI NotI NruI PacI PasI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI