Restriction map for
pF1KE6704
(Circular) (Six-base) MAPSORT of: pF1KE6704.seq Check: 1292 from: 1 to: 6296
pF1KE6704 6296 bp
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AatII G_ACGT'C
Cuts at: 1390 1580 1390
Size: 190 6106
AccI GT'mk_AC
Cuts at: 3334 3334
Size: 6296
Acc65I G'GTAC_C
Cuts at: 3312 3312
Size: 6296
AclI AA'CG_TT
Cuts at: 6136 6136
Size: 6296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 897 1932 2089 2323 2374 2716 4121 4553
Size: 695 1035 157 234 51 342 1405 432
Cuts at: 4553 5300 5568 202
Size: 747 268 930
Fragments arranged by size:
1405 1035 930 747 695 432 342 268
234 157 51
AfeI AGC'GCT
Cuts at: 2074 3254 2074
Size: 1180 5116
AflIII A'CryG_T
Cuts at: 1751 3648 4752 5729 1751
Size: 1897 1104 977 2318
Fragments arranged by size:
2318 1897 1104 977
AgeI A'CCGG_T
Cuts at: 572 4684 572
Size: 4112 2184
AleI CACnn'nnGTG
Cuts at: 1405 1405
Size: 6296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 796 2488 796
Size: 1692 4604
AlwNI CAG_nnn'CTG
Cuts at: 636 920 1359 1995 2094 2346 2658 2763 2859
Size: 284 439 636 99 252 312 105 96
Cuts at: 2859 3030 3601 5168 636
Size: 171 571 1567 1764
Fragments arranged by size:
1764 1567 636 571 439 312 284 252
171 105 99 96
ApaI G_GGCC'C
Cuts at: 1455 1455
Size: 6296
ApaLI G'TGCA_C
Cuts at: 2235 5066 2235
Size: 2831 3465
ApoI r'AATT_y
Cuts at: 514 1663 2418 3300 5649 5905 514
Size: 1149 755 882 2349 256 905
Fragments arranged by size:
2349 1149 905 882 755 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6296
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6296
AvaI C'yCGr_G
Cuts at: 166 727 964 2803 3316 166
Size: 561 237 1839 513 3146
Fragments arranged by size:
3146 1839 561 513 237
AvrII C'CTAG_G
Cuts at: 546 3135 546
Size: 2589 3707
BamHI G'GATC_C
Cuts at: 1889 3321 1889
Size: 1432 4864
BanI G'GyrC_C
Cuts at: 1037 2107 2360 2578 2663 3312 3726 3974 4009
Size: 1070 253 218 85 649 414 248 35
Cuts at: 4009 1037
Size: 3324
Fragments arranged by size:
3324 1070 649 414 253 248 218 85
35
BanII G_rGCy'C
Cuts at: 932 953 1004 1455 1514 1936 2887 3310 4340
Size: 21 51 451 59 422 951 423 1030
Cuts at: 4340 932
Size: 2888
Fragments arranged by size:
2888 1030 951 451 423 422 59 51
21
BbeI G_GCGC'C
Cuts at: 2111 3730 3978 2111
Size: 1619 248 4429
Fragments arranged by size:
4429 1619 248
BbsI GAAGACnn'nnnn_
Cuts at: 536 1260 1580 2138 536
Size: 724 320 558 4694
Fragments arranged by size:
4694 724 558 320
BbvCI CC'TCA_GC
Cuts at: 565 2993 565
Size: 2428 3868
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 3143 3177 90
Size: 34 3019 34 3209
Fragments arranged by size:
3209 3019 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 390 4194 4955 6296 390
Size: 3804 761 1341 390
Fragments arranged by size:
3804 1341 761 390
BclI T'GATC_A
Cuts at: 3816 3816
Size: 6296
BglI GCCn_nnn'nGGC
Cuts at: 2882 3182 2882
Size: 300 5996
BglII A'GATC_T
Cuts at: 2699 3811 5848 2699
Size: 1112 2037 3147
Fragments arranged by size:
3147 2037 1112
BlpI GC'TnA_GC
Cuts at: 2376 3172 3408 2376
Size: 796 236 5264
Fragments arranged by size:
5264 796 236
Bme1580I G_kGCm'C
Cuts at: 1455 2239 3921 4014 5070 1455
Size: 784 1682 93 1056 2681
Fragments arranged by size:
2681 1682 1056 784 93
BmgBI CAC'GTC
Cuts at: 1728 1728
Size: 6296
BmrI ACTGGGnnnn_n'
Cuts at: 816 2493 2662 2982 3508 3589 3924 6068 816
Size: 1677 169 320 526 81 335 2144 1044
Fragments arranged by size:
2144 1677 1044 526 335 320 169 81
BmtI G_CTAG'C
Cuts at: 683 683
Size: 6296
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 341 2409 2754 4663 341
Size: 2068 345 1909 1974
Fragments arranged by size:
2068 1974 1909 345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 336 928 1189 1333 1381 1447 1852 2355 2560
Size: 592 261 144 48 66 405 503 205
Cuts at: 2560 2644 2836 2902 3007 336
Size: 84 192 66 105 3625
Fragments arranged by size:
3625 592 503 405 261 205 192 144
105 84 66 66 48
Bpu10I CC'TnA_GC
Cuts at: 565 926 1109 1996 2779 2993 565
Size: 361 183 887 783 214 3868
Fragments arranged by size:
3868 887 783 361 214 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1671 2970 3472 4351 4843 5141 988
Size: 683 1299 502 879 492 298 2143
Fragments arranged by size:
2143 1299 879 683 502 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 691 1810 1860 2058 6000 691
Size: 1119 50 198 3942 987
Fragments arranged by size:
3942 1119 987 198 50
BsaAI yAC'GTr
Cuts at: 1278 2843 3649 1278
Size: 1565 806 3925
Fragments arranged by size:
3925 1565 806
BsaBI GATnn'nnATC
Cuts at: 6 3810 5847 6
Size: 3804 2037 455
Fragments arranged by size:
3804 2037 455
BsaHI Gr'CG_yC
Cuts at: 1387 1577 2108 2204 3727 3975 4677 6173 1387
Size: 190 531 96 1523 248 702 1496 1510
Fragments arranged by size:
1523 1510 1496 702 531 248 190 96
BsaWI w'CCGG_w
Cuts at: 572 1550 3485 4006 4684 4958 5105 5516 572
Size: 978 1935 521 678 274 147 411 1352
Fragments arranged by size:
1935 1352 978 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1963 1993 2017 2038 2047 2068 1963
Size: 30 24 21 9 21 6191
Fragments arranged by size:
6191 30 24 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 322 733 1066 1552 1828 1987 1990 2046 322
Size: 411 333 486 276 159 3 56 4572
Fragments arranged by size:
4572 486 411 333 276 159 56 3
BseYI C'CCAG_C
Cuts at: 219 278 931 1046 1328 2793 2880 3721 4479
Size: 59 653 115 282 1465 87 841 758
Cuts at: 4479 5056 5552 219
Size: 577 496 963
Fragments arranged by size:
1465 963 841 758 653 577 496 282
115 87 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2080 2194 2523 2080
Size: 114 329 5853
Fragments arranged by size:
5853 329 114
BsiEI CG_ry'CG
Cuts at: 104 3838 3884 5092 104
Size: 3734 46 1208 1308
Fragments arranged by size:
3734 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 605 953 1004 1175 1495 2048 2149 2239 2576
Size: 348 51 171 320 553 101 90 337
Cuts at: 2576 2918 3310 4088 4278 5070 605
Size: 342 392 778 190 792 1831
Fragments arranged by size:
1831 792 778 553 392 348 342 337
320 190 171 101 90 51
BsmI GAATG_Cn'
Cuts at: 895 895
Size: 6296
BsmBI CGTCTCn'nnnn_
Cuts at: 1584 1584
Size: 6296
Bsp1286I G_dGCh'C
Cuts at: 605 932 953 1004 1175 1455 1495 1514 1936
Size: 327 21 51 171 280 40 19 422
Cuts at: 1936 2048 2149 2239 2576 2887 2918 3310 3921
Size: 112 101 90 337 311 31 392 611
Cuts at: 3921 4014 4088 4278 4340 5070 605
Size: 93 74 190 62 730 1831
Fragments arranged by size:
1831 730 611 422 392 337 327 311
280 190 171 112 101 93 90 74
62 51 40 31 21 19
BspEI T'CCGG_A
Cuts at: 1550 5516 1550
Size: 3966 2330
BspHI T'CATG_A
Cuts at: 5472 5472
Size: 6296
BspMI ACCTGCnnnn'nnnn_
Cuts at: 609 1351 1430 3346 3560 3862 4243 609
Size: 742 79 1916 214 302 381 2662
Fragments arranged by size:
2662 1916 742 381 302 214 79
BsrBI CCG'CTC
Cuts at: 50 342 4588 4642 6126 6291 50
Size: 292 4246 54 1484 165 55
Fragments arranged by size:
4246 1484 292 165 55 54
BsrDI GCAATG_nn'
Cuts at: 543 2027 4208 543
Size: 1484 2181 2631
Fragments arranged by size:
2631 2181 1484
BsrFI r'CCGG_y
Cuts at: 259 572 2876 4294 4475 4684 259
Size: 313 2304 1418 181 209 1871
Fragments arranged by size:
2304 1871 1418 313 209 181
BsrGI T'GTAC_A
Cuts at: 1409 1409
Size: 6296
BssHII G'CGCG_C
Cuts at: 541 935 541
Size: 394 5902
BssSI C'ACGA_G
Cuts at: 4567 4925 4567
Size: 358 5938
BstAPI GCAn_nnn'nTGC
Cuts at: 3673 3673
Size: 6296
BstBI TT'CG_AA
Cuts at: 4657 4657
Size: 6296
BstXI CCAn_nnnn'nTGG
Cuts at: 156 1146 1185 156
Size: 990 39 5267
Fragments arranged by size:
5267 990 39
BstYI r'GATC_y
Cuts at: 1 233 477 1190 1889 1964 2433 2699 3321
Size: 232 244 713 699 75 469 266 622
Cuts at: 3321 3788 3811 4146 4392 5404 5490 5502 5848
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5848 1
Size: 449
Fragments arranged by size:
1012 713 699 622 469 467 449 346
335 266 246 244 232 86 75 23
12
Bsu36I CC'TnA_GG
Cuts at: 1025 2347 2638 2830 1025
Size: 1322 291 192 4491
Fragments arranged by size:
4491 1322 291 192
BtgI C'CryG_G
Cuts at: 665 743 884 1064 1139 665
Size: 78 141 180 75 5822
Fragments arranged by size:
5822 180 141 78 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4246 4427 4590 4246
Size: 181 163 5952
Fragments arranged by size:
5952 181 163
BtsI GCAGTG_nn'
Cuts at: 1400 1412 1891 2711 3539 1400
Size: 12 479 820 828 4157
Fragments arranged by size:
4157 828 820 479 12
DraI TTT'AAA
Cuts at: 3295 3295
Size: 6296
DraIII CAC_nnn'GTG
Cuts at: 409 795 1307 409
Size: 386 512 5398
Fragments arranged by size:
5398 512 386
DrdI GACnn_nn'nnGTC
Cuts at: 3148 4002 4860 3148
Size: 854 858 4584
Fragments arranged by size:
4584 858 854
EaeI y'GGCC_r
Cuts at: 3881 4055 4446 4473 5818 3881
Size: 174 391 27 1345 4359
Fragments arranged by size:
4359 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3881 3881
Size: 6296
EarI CTCTTCn'nnn_
Cuts at: 829 853 1303 1831 2350 4319 4529 829
Size: 24 450 528 519 1969 210 2596
Fragments arranged by size:
2596 1969 528 519 450 210 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 826 4504 4814 4960 6106 826
Size: 3678 310 146 1146 1016
Fragments arranged by size:
3678 1146 1016 310 146
EcoICRI GAG'CTC
Cuts at: 951 1002 3308 951
Size: 51 2306 3939
Fragments arranged by size:
3939 2306 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 336 897 928 1189 1333 1381 1447 1852
Size: 134 561 31 261 144 48 66 405
Cuts at: 1852 1932 2089 2323 2355 2374 2560 2644 2716
Size: 80 157 234 32 19 186 84 72
Cuts at: 2716 2836 2902 3007 4121 4553 5300 5568 202
Size: 120 66 105 1114 432 747 268 930
Fragments arranged by size:
1114 930 747 561 432 405 268 261
234 186 157 144 134 120 105 84
80 72 66 66 48 32 31 19
EcoNI CCTnn'n_nnAGG
Cuts at: 1534 1693 1810 1927 1534
Size: 159 117 117 5903
Fragments arranged by size:
5903 159 117 117
EcoO109I rG'GnC_Cy
Cuts at: 357 384 1030 1451 1452 1545 1992 2388 2484
Size: 27 646 421 1 93 447 396 96
Cuts at: 2484 2799 2826 2976 3435 357
Size: 315 27 150 459 3218
Fragments arranged by size:
3218 646 459 447 421 396 315 150
96 93 27 27 1
EcoRI G'AATT_C
Cuts at: 1663 3300 1663
Size: 1637 4659
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 475 1242 4315 475
Size: 767 3073 2456
Fragments arranged by size:
3073 2456 767
FspI TGC'GCA
Cuts at: 4077 4077
Size: 6296
HaeII r_GCGC'y
Cuts at: 726 972 2076 2087 2111 3256 3730 3978 5000
Size: 246 1104 11 24 1145 474 248 1022
Cuts at: 5000 5979 726
Size: 979 1043
Fragments arranged by size:
1145 1104 1043 1022 979 474 248 246
24 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2517 2549 4138 4168 4170 4200 2517
Size: 32 1589 30 2 30 4613
Fragments arranged by size:
4613 1589 32 30 30 2
HincII GTy'rAC
Cuts at: 437 561 1742 3335 437
Size: 124 1181 1593 3398
Fragments arranged by size:
3398 1593 1181 124
KasI G'GCGC_C
Cuts at: 2107 3726 3974 2107
Size: 1619 248 4429
Fragments arranged by size:
4429 1619 248
KpnI G_GTAC'C
Cuts at: 3316 3316
Size: 6296
MluI A'CGCG_T
Cuts at: 1751 5729 1751
Size: 3978 2318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2433 2582 3177 4967 5151 2433
Size: 149 595 1790 184 3578
Fragments arranged by size:
3578 1790 595 184 149
MscI TGG'CCA
Cuts at: 4057 4057
Size: 6296
MslI CAynn'nnrTG
Cuts at: 1405 1870 2102 4285 4412 1405
Size: 465 232 2183 127 3289
Fragments arranged by size:
3289 2183 465 232 127
MspA1I CmG'CkG
Cuts at: 282 917 1359 1952 2227 2538 3407 3721 4081
Size: 635 442 593 275 311 869 314 360
Cuts at: 4081 5094 5339 282
Size: 1013 245 1239
Fragments arranged by size:
1239 1013 869 635 593 442 360 314
311 275 245
NaeI GCC'GGC
Cuts at: 2878 4477 2878
Size: 1599 4697
NarI GG'CG_CC
Cuts at: 2108 3727 3975 2108
Size: 1619 248 4429
Fragments arranged by size:
4429 1619 248
NgoMIV G'CCGG_C
Cuts at: 2876 4475 2876
Size: 1599 4697
NheI G'CTAG_C
Cuts at: 679 679
Size: 6296
NspI r_CATG'y
Cuts at: 443 900 1186 1416 3349 3849 4756 443
Size: 457 286 230 1933 500 907 1983
Fragments arranged by size:
1983 1933 907 500 457 286 230
PasI CC'CwG_GG
Cuts at: 239 2280 3087 239
Size: 2041 807 3448
Fragments arranged by size:
3448 2041 807
PciI A'CATG_T
Cuts at: 4752 4752
Size: 6296
PflMI CCAn_nnn'nTGG
Cuts at: 409 409
Size: 6296
PfoI T'CCnGG_A
Cuts at: 944 1390 1658 1688 1967 2936 944
Size: 446 268 30 279 969 4304
Fragments arranged by size:
4304 969 446 279 268 30
PmeI GTTT'AAAC
Cuts at: 3295 3295
Size: 6296
PmlI CAC'GTG
Cuts at: 2843 2843
Size: 6296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5435 5467 5435
Size: 32 6264
PpuMI rG'GwC_Cy
Cuts at: 357 384 1030 1545 2388 2799 357
Size: 27 646 515 843 411 3854
Fragments arranged by size:
3854 843 646 515 411 27
PshAI GACnn'nnGTC
Cuts at: 1386 1386
Size: 6296
PspOMI G'GGCC_C
Cuts at: 1451 1451
Size: 6296
PspXI vC'TCGA_Gb
Cuts at: 166 166
Size: 6296
PstI C_TGCA'G
Cuts at: 865 1407 1928 2080 2400 2513 2677 3019 3343
Size: 542 521 152 320 113 164 342 324
Cuts at: 3343 4028 865
Size: 685 3133
Fragments arranged by size:
3133 685 542 521 342 324 320 164
152 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6296
PvuII CAG'CTG
Cuts at: 282 917 1359 1952 2538 3721 4081 282
Size: 635 442 593 586 1183 360 2497
Fragments arranged by size:
2497 1183 635 593 586 442 360
RsrII CG'GwC_CG
Cuts at: 4491 4491
Size: 6296
SacI G_AGCT'C
Cuts at: 953 1004 3310 953
Size: 51 2306 3939
Fragments arranged by size:
3939 2306 51
SalI G'TCGA_C
Cuts at: 3333 3333
Size: 6296
SanDI GG'GwC_CC
Cuts at: 1030 1030
Size: 6296
SapI GCTCTTCn'nnn_
Cuts at: 829 4319 4529 829
Size: 3490 210 2596
Fragments arranged by size:
3490 2596 210
SbfI CC_TGCA'GG
Cuts at: 3343 3343
Size: 6296
ScaI AGT'ACT
Cuts at: 5813 5813
Size: 6296
SexAI A'CCwGG_T
Cuts at: 386 405 386
Size: 19 6277
SfcI C'TryA_G
Cuts at: 33 492 861 1403 1635 1924 2076 2115 2208
Size: 459 369 542 232 289 152 39 93
Cuts at: 2208 2396 2509 2673 3015 3339 4024 5017 5208
Size: 188 113 164 342 324 685 993 191
Cuts at: 5208 33
Size: 1121
Fragments arranged by size:
1121 993 685 542 459 369 342 324
289 232 191 188 164 152 113 93
39
SfoI GGC'GCC
Cuts at: 2109 3728 3976 2109
Size: 1619 248 4429
Fragments arranged by size:
4429 1619 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6296
SmaI CCC'GGG
Cuts at: 3318 3318
Size: 6296
SmlI C'TyrA_G
Cuts at: 166 1003 1650 2949 3451 4366 4858 5120 166
Size: 837 647 1299 502 915 492 262 1342
Fragments arranged by size:
1342 1299 915 837 647 502 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6296
SphI G_CATG'C
Cuts at: 900 3349 3849 900
Size: 2449 500 3347
Fragments arranged by size:
3347 2449 500
StuI AGG'CCT
Cuts at: 313 313
Size: 6296
StyI C'CwwG_G
Cuts at: 546 828 1496 1937 3059 3135 3430 3784 546
Size: 282 668 441 1122 76 295 354 3058
Fragments arranged by size:
3058 1122 668 441 354 295 282 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 307 1320 1488 1759 2681 3124 3879 4012 4471
Size: 1013 168 271 922 443 755 133 459
Cuts at: 4471 4680 307
Size: 209 1923
Fragments arranged by size:
1923 1013 922 755 459 443 271 209
168 133
TatI w'GTAC_w
Cuts at: 1409 1679 5811 1409
Size: 270 4132 1894
Fragments arranged by size:
4132 1894 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2038 2070 5813 5845 2038
Size: 32 3743 32 2489
Fragments arranged by size:
3743 2489 32 32
Tth111I GACn'n_nGTC
Cuts at: 502 4093 502
Size: 3591 2705
XbaI T'CTAG_A
Cuts at: 3327 3327
Size: 6296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 311 1827 2589 3791 311
Size: 1516 762 1202 2816
Fragments arranged by size:
2816 1516 1202 762
XhoI C'TCGA_G
Cuts at: 166 166
Size: 6296
XmaI C'CCGG_G
Cuts at: 3316 3316
Size: 6296
XmnI GAAnn'nnTTC
Cuts at: 187 823 1603 187
Size: 636 780 4880
Fragments arranged by size:
4880 780 636
ZraI GAC'GTC
Cuts at: 1388 1578 1388
Size: 190 6106
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AloI AscI BaeI BfrBI BsiWI
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NotI NruI NsiI PacI
PsiI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
SwaI