Restriction map for pF1KE6704
 (Circular) (Six-base) MAPSORT of: pF1KE6704.seq  Check: 1292  from: 1  to: 6296



pF1KE6704  6296 bp

 With 182 enzymes: SgfI * 

                        June 19, 2019 14:13  ..

AatII G_ACGT'C

Cuts at:   1390    1580    1390 
   Size:        190    6106
 
AccI GT'mk_AC

Cuts at:   3334    3334 
   Size:       6296
 
Acc65I G'GTAC_C

Cuts at:   3312    3312 
   Size:       6296
 
AclI AA'CG_TT

Cuts at:   6136    6136 
   Size:       6296
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    202     897    1932    2089    2323    2374    2716    4121    4553 
   Size:        695    1035     157     234      51     342    1405     432

Cuts at:   4553    5300    5568     202 
   Size:        747     268     930

  Fragments arranged by size: 

                1405    1035     930     747     695     432     342     268
                 234     157      51
 
AfeI AGC'GCT

Cuts at:   2074    3254    2074 
   Size:       1180    5116
 
AflIII A'CryG_T

Cuts at:   1751    3648    4752    5729    1751 
   Size:       1897    1104     977    2318

  Fragments arranged by size: 

                2318    1897    1104     977
 
AgeI A'CCGG_T

Cuts at:    572    4684     572 
   Size:       4112    2184
 
AleI CACnn'nnGTG

Cuts at:   1405    1405 
   Size:       6296
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    796    2488     796 
   Size:       1692    4604
 
AlwNI CAG_nnn'CTG

Cuts at:    636     920    1359    1995    2094    2346    2658    2763    2859 
   Size:        284     439     636      99     252     312     105      96

Cuts at:   2859    3030    3601    5168     636 
   Size:        171     571    1567    1764

  Fragments arranged by size: 

                1764    1567     636     571     439     312     284     252
                 171     105      99      96
 
ApaI G_GGCC'C

Cuts at:   1455    1455 
   Size:       6296
 
ApaLI G'TGCA_C

Cuts at:   2235    5066    2235 
   Size:       2831    3465
 
ApoI r'AATT_y

Cuts at:    514    1663    2418    3300    5649    5905     514 
   Size:       1149     755     882    2349     256     905

  Fragments arranged by size: 

                2349    1149     905     882     755     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6296
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6296
 
AvaI C'yCGr_G

Cuts at:    166     727     964    2803    3316     166 
   Size:        561     237    1839     513    3146

  Fragments arranged by size: 

                3146    1839     561     513     237
 
AvrII C'CTAG_G

Cuts at:    546    3135     546 
   Size:       2589    3707
 
BamHI G'GATC_C

Cuts at:   1889    3321    1889 
   Size:       1432    4864
 
BanI G'GyrC_C

Cuts at:   1037    2107    2360    2578    2663    3312    3726    3974    4009 
   Size:       1070     253     218      85     649     414     248      35

Cuts at:   4009    1037 
   Size:       3324

  Fragments arranged by size: 

                3324    1070     649     414     253     248     218      85
                  35
 
BanII G_rGCy'C

Cuts at:    932     953    1004    1455    1514    1936    2887    3310    4340 
   Size:         21      51     451      59     422     951     423    1030

Cuts at:   4340     932 
   Size:       2888

  Fragments arranged by size: 

                2888    1030     951     451     423     422      59      51
                  21
 
BbeI G_GCGC'C

Cuts at:   2111    3730    3978    2111 
   Size:       1619     248    4429

  Fragments arranged by size: 

                4429    1619     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    536    1260    1580    2138     536 
   Size:        724     320     558    4694

  Fragments arranged by size: 

                4694     724     558     320
 
BbvCI CC'TCA_GC

Cuts at:    565    2993     565 
   Size:       2428    3868
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124    3143    3177      90 
   Size:         34    3019      34    3209

  Fragments arranged by size: 

                3209    3019      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:    390    4194    4955    6296     390 
   Size:       3804     761    1341     390

  Fragments arranged by size: 

                3804    1341     761     390
 
BclI T'GATC_A

Cuts at:   3816    3816 
   Size:       6296
 
BglI GCCn_nnn'nGGC

Cuts at:   2882    3182    2882 
   Size:        300    5996
 
BglII A'GATC_T

Cuts at:   2699    3811    5848    2699 
   Size:       1112    2037    3147

  Fragments arranged by size: 

                3147    2037    1112
 
BlpI GC'TnA_GC

Cuts at:   2376    3172    3408    2376 
   Size:        796     236    5264

  Fragments arranged by size: 

                5264     796     236
 
Bme1580I G_kGCm'C

Cuts at:   1455    2239    3921    4014    5070    1455 
   Size:        784    1682      93    1056    2681

  Fragments arranged by size: 

                2681    1682    1056     784      93
 
BmgBI CAC'GTC

Cuts at:   1728    1728 
   Size:       6296
 
BmrI ACTGGGnnnn_n'

Cuts at:    816    2493    2662    2982    3508    3589    3924    6068     816 
   Size:       1677     169     320     526      81     335    2144    1044

  Fragments arranged by size: 

                2144    1677    1044     526     335     320     169      81
            
 
BmtI G_CTAG'C

Cuts at:    683     683 
   Size:       6296
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    341    2409    2754    4663     341 
   Size:       2068     345    1909    1974

  Fragments arranged by size: 

                2068    1974    1909     345
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    336     928    1189    1333    1381    1447    1852    2355    2560 
   Size:        592     261     144      48      66     405     503     205

Cuts at:   2560    2644    2836    2902    3007     336 
   Size:         84     192      66     105    3625

  Fragments arranged by size: 

                3625     592     503     405     261     205     192     144
                 105      84      66      66      48
 
Bpu10I CC'TnA_GC

Cuts at:    565     926    1109    1996    2779    2993     565 
   Size:        361     183     887     783     214    3868

  Fragments arranged by size: 

                3868     887     783     361     214     183
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    988    1671    2970    3472    4351    4843    5141     988 
   Size:        683    1299     502     879     492     298    2143

  Fragments arranged by size: 

                2143    1299     879     683     502     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    691    1810    1860    2058    6000     691 
   Size:       1119      50     198    3942     987

  Fragments arranged by size: 

                3942    1119     987     198      50
 
BsaAI yAC'GTr

Cuts at:   1278    2843    3649    1278 
   Size:       1565     806    3925

  Fragments arranged by size: 

                3925    1565     806
 
BsaBI GATnn'nnATC

Cuts at:      6    3810    5847       6 
   Size:       3804    2037     455

  Fragments arranged by size: 

                3804    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1387    1577    2108    2204    3727    3975    4677    6173    1387 
   Size:        190     531      96    1523     248     702    1496    1510

  Fragments arranged by size: 

                1523    1510    1496     702     531     248     190      96
            
 
BsaWI w'CCGG_w

Cuts at:    572    1550    3485    4006    4684    4958    5105    5516     572 
   Size:        978    1935     521     678     274     147     411    1352

  Fragments arranged by size: 

                1935    1352     978     678     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1963    1993    2017    2038    2047    2068    1963 
   Size:         30      24      21       9      21    6191

  Fragments arranged by size: 

                6191      30      24      21      21       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    322     733    1066    1552    1828    1987    1990    2046     322 
   Size:        411     333     486     276     159       3      56    4572

  Fragments arranged by size: 

                4572     486     411     333     276     159      56       3
            
 
BseYI C'CCAG_C

Cuts at:    219     278     931    1046    1328    2793    2880    3721    4479 
   Size:         59     653     115     282    1465      87     841     758

Cuts at:   4479    5056    5552     219 
   Size:        577     496     963

  Fragments arranged by size: 

                1465     963     841     758     653     577     496     282
                 115      87      59
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   2080    2194    2523    2080 
   Size:        114     329    5853

  Fragments arranged by size: 

                5853     329     114
 
BsiEI CG_ry'CG

Cuts at:    104    3838    3884    5092     104 
   Size:       3734      46    1208    1308

  Fragments arranged by size: 

                3734    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    605     953    1004    1175    1495    2048    2149    2239    2576 
   Size:        348      51     171     320     553     101      90     337

Cuts at:   2576    2918    3310    4088    4278    5070     605 
   Size:        342     392     778     190     792    1831

  Fragments arranged by size: 

                1831     792     778     553     392     348     342     337
                 320     190     171     101      90      51
 
BsmI GAATG_Cn'

Cuts at:    895     895 
   Size:       6296
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1584    1584 
   Size:       6296
 
Bsp1286I G_dGCh'C

Cuts at:    605     932     953    1004    1175    1455    1495    1514    1936 
   Size:        327      21      51     171     280      40      19     422

Cuts at:   1936    2048    2149    2239    2576    2887    2918    3310    3921 
   Size:        112     101      90     337     311      31     392     611

Cuts at:   3921    4014    4088    4278    4340    5070     605 
   Size:         93      74     190      62     730    1831

  Fragments arranged by size: 

                1831     730     611     422     392     337     327     311
                 280     190     171     112     101      93      90      74
                  62      51      40      31      21      19
 
BspEI T'CCGG_A

Cuts at:   1550    5516    1550 
   Size:       3966    2330
 
BspHI T'CATG_A

Cuts at:   5472    5472 
   Size:       6296
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    609    1351    1430    3346    3560    3862    4243     609 
   Size:        742      79    1916     214     302     381    2662

  Fragments arranged by size: 

                2662    1916     742     381     302     214      79
 
BsrBI CCG'CTC

Cuts at:     50     342    4588    4642    6126    6291      50 
   Size:        292    4246      54    1484     165      55

  Fragments arranged by size: 

                4246    1484     292     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    543    2027    4208     543 
   Size:       1484    2181    2631

  Fragments arranged by size: 

                2631    2181    1484
 
BsrFI r'CCGG_y

Cuts at:    259     572    2876    4294    4475    4684     259 
   Size:        313    2304    1418     181     209    1871

  Fragments arranged by size: 

                2304    1871    1418     313     209     181
 
BsrGI T'GTAC_A

Cuts at:   1409    1409 
   Size:       6296
 
BssHII G'CGCG_C

Cuts at:    541     935     541 
   Size:        394    5902
 
BssSI C'ACGA_G

Cuts at:   4567    4925    4567 
   Size:        358    5938
 
BstAPI GCAn_nnn'nTGC

Cuts at:   3673    3673 
   Size:       6296
 
BstBI TT'CG_AA

Cuts at:   4657    4657 
   Size:       6296
 
BstXI CCAn_nnnn'nTGG

Cuts at:    156    1146    1185     156 
   Size:        990      39    5267

  Fragments arranged by size: 

                5267     990      39
 
BstYI r'GATC_y

Cuts at:      1     233     477    1190    1889    1964    2433    2699    3321 
   Size:        232     244     713     699      75     469     266     622

Cuts at:   3321    3788    3811    4146    4392    5404    5490    5502    5848 
   Size:        467      23     335     246    1012      86      12     346

Cuts at:   5848       1 
   Size:        449

  Fragments arranged by size: 

                1012     713     699     622     469     467     449     346
                 335     266     246     244     232      86      75      23
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   1025    2347    2638    2830    1025 
   Size:       1322     291     192    4491

  Fragments arranged by size: 

                4491    1322     291     192
 
BtgI C'CryG_G

Cuts at:    665     743     884    1064    1139     665 
   Size:         78     141     180      75    5822

  Fragments arranged by size: 

                5822     180     141      78      75
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   4246    4427    4590    4246 
   Size:        181     163    5952

  Fragments arranged by size: 

                5952     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1400    1412    1891    2711    3539    1400 
   Size:         12     479     820     828    4157

  Fragments arranged by size: 

                4157     828     820     479      12
 
DraI TTT'AAA

Cuts at:   3295    3295 
   Size:       6296
 
DraIII CAC_nnn'GTG

Cuts at:    409     795    1307     409 
   Size:        386     512    5398

  Fragments arranged by size: 

                5398     512     386
 
DrdI GACnn_nn'nnGTC

Cuts at:   3148    4002    4860    3148 
   Size:        854     858    4584

  Fragments arranged by size: 

                4584     858     854
 
EaeI y'GGCC_r

Cuts at:   3881    4055    4446    4473    5818    3881 
   Size:        174     391      27    1345    4359

  Fragments arranged by size: 

                4359    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   3881    3881 
   Size:       6296
 
EarI CTCTTCn'nnn_

Cuts at:    829     853    1303    1831    2350    4319    4529     829 
   Size:         24     450     528     519    1969     210    2596

  Fragments arranged by size: 

                2596    1969     528     519     450     210      24
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    826    4504    4814    4960    6106     826 
   Size:       3678     310     146    1146    1016

  Fragments arranged by size: 

                3678    1146    1016     310     146
 
EcoICRI GAG'CTC

Cuts at:    951    1002    3308     951 
   Size:         51    2306    3939

  Fragments arranged by size: 

                3939    2306      51
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    202     336     897     928    1189    1333    1381    1447    1852 
   Size:        134     561      31     261     144      48      66     405

Cuts at:   1852    1932    2089    2323    2355    2374    2560    2644    2716 
   Size:         80     157     234      32      19     186      84      72

Cuts at:   2716    2836    2902    3007    4121    4553    5300    5568     202 
   Size:        120      66     105    1114     432     747     268     930

  Fragments arranged by size: 

                1114     930     747     561     432     405     268     261
                 234     186     157     144     134     120     105      84
                  80      72      66      66      48      32      31      19
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1534    1693    1810    1927    1534 
   Size:        159     117     117    5903

  Fragments arranged by size: 

                5903     159     117     117
 
EcoO109I rG'GnC_Cy

Cuts at:    357     384    1030    1451    1452    1545    1992    2388    2484 
   Size:         27     646     421       1      93     447     396      96

Cuts at:   2484    2799    2826    2976    3435     357 
   Size:        315      27     150     459    3218

  Fragments arranged by size: 

                3218     646     459     447     421     396     315     150
                  96      93      27      27       1
 
EcoRI G'AATT_C

Cuts at:   1663    3300    1663 
   Size:       1637    4659
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    475    1242    4315     475 
   Size:        767    3073    2456

  Fragments arranged by size: 

                3073    2456     767
 
FspI TGC'GCA

Cuts at:   4077    4077 
   Size:       6296
 
HaeII r_GCGC'y

Cuts at:    726     972    2076    2087    2111    3256    3730    3978    5000 
   Size:        246    1104      11      24    1145     474     248    1022

Cuts at:   5000    5979     726 
   Size:        979    1043

  Fragments arranged by size: 

                1145    1104    1043    1022     979     474     248     246
                  24      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2517    2549    4138    4168    4170    4200    2517 
   Size:         32    1589      30       2      30    4613

  Fragments arranged by size: 

                4613    1589      32      30      30       2
 
HincII GTy'rAC

Cuts at:    437     561    1742    3335     437 
   Size:        124    1181    1593    3398

  Fragments arranged by size: 

                3398    1593    1181     124
 
KasI G'GCGC_C

Cuts at:   2107    3726    3974    2107 
   Size:       1619     248    4429

  Fragments arranged by size: 

                4429    1619     248
 
KpnI G_GTAC'C

Cuts at:   3316    3316 
   Size:       6296
 
MluI A'CGCG_T

Cuts at:   1751    5729    1751 
   Size:       3978    2318
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2433    2582    3177    4967    5151    2433 
   Size:        149     595    1790     184    3578

  Fragments arranged by size: 

                3578    1790     595     184     149
 
MscI TGG'CCA

Cuts at:   4057    4057 
   Size:       6296
 
MslI CAynn'nnrTG

Cuts at:   1405    1870    2102    4285    4412    1405 
   Size:        465     232    2183     127    3289

  Fragments arranged by size: 

                3289    2183     465     232     127
 
MspA1I CmG'CkG

Cuts at:    282     917    1359    1952    2227    2538    3407    3721    4081 
   Size:        635     442     593     275     311     869     314     360

Cuts at:   4081    5094    5339     282 
   Size:       1013     245    1239

  Fragments arranged by size: 

                1239    1013     869     635     593     442     360     314
                 311     275     245
 
NaeI GCC'GGC

Cuts at:   2878    4477    2878 
   Size:       1599    4697
 
NarI GG'CG_CC

Cuts at:   2108    3727    3975    2108 
   Size:       1619     248    4429

  Fragments arranged by size: 

                4429    1619     248
 
NgoMIV G'CCGG_C

Cuts at:   2876    4475    2876 
   Size:       1599    4697
 
NheI G'CTAG_C

Cuts at:    679     679 
   Size:       6296
 
NspI r_CATG'y

Cuts at:    443     900    1186    1416    3349    3849    4756     443 
   Size:        457     286     230    1933     500     907    1983

  Fragments arranged by size: 

                1983    1933     907     500     457     286     230
 
PasI CC'CwG_GG

Cuts at:    239    2280    3087     239 
   Size:       2041     807    3448

  Fragments arranged by size: 

                3448    2041     807
 
PciI A'CATG_T

Cuts at:   4752    4752 
   Size:       6296
 
PflMI CCAn_nnn'nTGG

Cuts at:    409     409 
   Size:       6296
 
PfoI T'CCnGG_A

Cuts at:    944    1390    1658    1688    1967    2936     944 
   Size:        446     268      30     279     969    4304

  Fragments arranged by size: 

                4304     969     446     279     268      30
 
PmeI GTTT'AAAC

Cuts at:   3295    3295 
   Size:       6296
 
PmlI CAC'GTG

Cuts at:   2843    2843 
   Size:       6296
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5435    5467    5435 
   Size:         32    6264
 
PpuMI rG'GwC_Cy

Cuts at:    357     384    1030    1545    2388    2799     357 
   Size:         27     646     515     843     411    3854

  Fragments arranged by size: 

                3854     843     646     515     411      27
 
PshAI GACnn'nnGTC

Cuts at:   1386    1386 
   Size:       6296
 
PspOMI G'GGCC_C

Cuts at:   1451    1451 
   Size:       6296
 
PspXI vC'TCGA_Gb

Cuts at:    166     166 
   Size:       6296
 
PstI C_TGCA'G

Cuts at:    865    1407    1928    2080    2400    2513    2677    3019    3343 
   Size:        542     521     152     320     113     164     342     324

Cuts at:   3343    4028     865 
   Size:        685    3133

  Fragments arranged by size: 

                3133     685     542     521     342     324     320     164
                 152     113
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6296
 
PvuII CAG'CTG

Cuts at:    282     917    1359    1952    2538    3721    4081     282 
   Size:        635     442     593     586    1183     360    2497

  Fragments arranged by size: 

                2497    1183     635     593     586     442     360
 
RsrII CG'GwC_CG

Cuts at:   4491    4491 
   Size:       6296
 
SacI G_AGCT'C

Cuts at:    953    1004    3310     953 
   Size:         51    2306    3939

  Fragments arranged by size: 

                3939    2306      51
 
SalI G'TCGA_C

Cuts at:   3333    3333 
   Size:       6296
 
SanDI GG'GwC_CC

Cuts at:   1030    1030 
   Size:       6296
 
SapI GCTCTTCn'nnn_

Cuts at:    829    4319    4529     829 
   Size:       3490     210    2596

  Fragments arranged by size: 

                3490    2596     210
 
SbfI CC_TGCA'GG

Cuts at:   3343    3343 
   Size:       6296
 
ScaI AGT'ACT

Cuts at:   5813    5813 
   Size:       6296
 
SexAI A'CCwGG_T

Cuts at:    386     405     386 
   Size:         19    6277
 
SfcI C'TryA_G

Cuts at:     33     492     861    1403    1635    1924    2076    2115    2208 
   Size:        459     369     542     232     289     152      39      93

Cuts at:   2208    2396    2509    2673    3015    3339    4024    5017    5208 
   Size:        188     113     164     342     324     685     993     191

Cuts at:   5208      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     685     542     459     369     342     324
                 289     232     191     188     164     152     113      93
                  39
 
SfoI GGC'GCC

Cuts at:   2109    3728    3976    2109 
   Size:       1619     248    4429

  Fragments arranged by size: 

                4429    1619     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6296
 
SmaI CCC'GGG

Cuts at:   3318    3318 
   Size:       6296
 
SmlI C'TyrA_G

Cuts at:    166    1003    1650    2949    3451    4366    4858    5120     166 
   Size:        837     647    1299     502     915     492     262    1342

  Fragments arranged by size: 

                1342    1299     915     837     647     502     492     262
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6296
 
SphI G_CATG'C

Cuts at:    900    3349    3849     900 
   Size:       2449     500    3347

  Fragments arranged by size: 

                3347    2449     500
 
StuI AGG'CCT

Cuts at:    313     313 
   Size:       6296
 
StyI C'CwwG_G

Cuts at:    546     828    1496    1937    3059    3135    3430    3784     546 
   Size:        282     668     441    1122      76     295     354    3058

  Fragments arranged by size: 

                3058    1122     668     441     354     295     282      76
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    307    1320    1488    1759    2681    3124    3879    4012    4471 
   Size:       1013     168     271     922     443     755     133     459

Cuts at:   4471    4680     307 
   Size:        209    1923

  Fragments arranged by size: 

                1923    1013     922     755     459     443     271     209
                 168     133
 
TatI w'GTAC_w

Cuts at:   1409    1679    5811    1409 
   Size:        270    4132    1894

  Fragments arranged by size: 

                4132    1894     270
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2038    2070    5813    5845    2038 
   Size:         32    3743      32    2489

  Fragments arranged by size: 

                3743    2489      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    502    4093     502 
   Size:       3591    2705
 
XbaI T'CTAG_A

Cuts at:   3327    3327 
   Size:       6296
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    311    1827    2589    3791     311 
   Size:       1516     762    1202    2816

  Fragments arranged by size: 

                2816    1516    1202     762
 
XhoI C'TCGA_G

Cuts at:    166     166 
   Size:       6296
 
XmaI C'CCGG_G

Cuts at:   3316    3316 
   Size:       6296
 
XmnI GAAnn'nnTTC

Cuts at:    187     823    1603     187 
   Size:        636     780    4880

  Fragments arranged by size: 

                4880     780     636
 
ZraI GAC'GTC

Cuts at:   1388    1578    1388 
   Size:        190    6106
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NheI     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI    AflII     AhdI     AloI     AscI     BaeI    BfrBI    BsiWI
   BstEII  BstZ17I     ClaI    CspCI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NcoI     NdeI     NotI     NruI     NsiI     PacI
     PsiI     PsrI    SacII     SfiI    SgrAI    SnaBI     SrfI     SspI
     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com