(Circular) (Six-base) MAPSORT of: pF1KE6704.seq Check: 1292 from: 1 to: 6296 pF1KE6704 6296 bp With 182 enzymes: SgfI * June 19, 2019 14:13 .. AatII G_ACGT'C Cuts at: 1390 1580 1390 Size: 190 6106 AccI GT'mk_AC Cuts at: 3334 3334 Size: 6296 Acc65I G'GTAC_C Cuts at: 3312 3312 Size: 6296 AclI AA'CG_TT Cuts at: 6136 6136 Size: 6296 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 202 897 1932 2089 2323 2374 2716 4121 4553 Size: 695 1035 157 234 51 342 1405 432 Cuts at: 4553 5300 5568 202 Size: 747 268 930 Fragments arranged by size: 1405 1035 930 747 695 432 342 268 234 157 51 AfeI AGC'GCT Cuts at: 2074 3254 2074 Size: 1180 5116 AflIII A'CryG_T Cuts at: 1751 3648 4752 5729 1751 Size: 1897 1104 977 2318 Fragments arranged by size: 2318 1897 1104 977 AgeI A'CCGG_T Cuts at: 572 4684 572 Size: 4112 2184 AleI CACnn'nnGTG Cuts at: 1405 1405 Size: 6296 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 796 2488 796 Size: 1692 4604 AlwNI CAG_nnn'CTG Cuts at: 636 920 1359 1995 2094 2346 2658 2763 2859 Size: 284 439 636 99 252 312 105 96 Cuts at: 2859 3030 3601 5168 636 Size: 171 571 1567 1764 Fragments arranged by size: 1764 1567 636 571 439 312 284 252 171 105 99 96 ApaI G_GGCC'C Cuts at: 1455 1455 Size: 6296 ApaLI G'TGCA_C Cuts at: 2235 5066 2235 Size: 2831 3465 ApoI r'AATT_y Cuts at: 514 1663 2418 3300 5649 5905 514 Size: 1149 755 882 2349 256 905 Fragments arranged by size: 2349 1149 905 882 755 256 AseI AT'TA_AT Cuts at: 20 20 Size: 6296 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6296 AvaI C'yCGr_G Cuts at: 166 727 964 2803 3316 166 Size: 561 237 1839 513 3146 Fragments arranged by size: 3146 1839 561 513 237 AvrII C'CTAG_G Cuts at: 546 3135 546 Size: 2589 3707 BamHI G'GATC_C Cuts at: 1889 3321 1889 Size: 1432 4864 BanI G'GyrC_C Cuts at: 1037 2107 2360 2578 2663 3312 3726 3974 4009 Size: 1070 253 218 85 649 414 248 35 Cuts at: 4009 1037 Size: 3324 Fragments arranged by size: 3324 1070 649 414 253 248 218 85 35 BanII G_rGCy'C Cuts at: 932 953 1004 1455 1514 1936 2887 3310 4340 Size: 21 51 451 59 422 951 423 1030 Cuts at: 4340 932 Size: 2888 Fragments arranged by size: 2888 1030 951 451 423 422 59 51 21 BbeI G_GCGC'C Cuts at: 2111 3730 3978 2111 Size: 1619 248 4429 Fragments arranged by size: 4429 1619 248 BbsI GAAGACnn'nnnn_ Cuts at: 536 1260 1580 2138 536 Size: 724 320 558 4694 Fragments arranged by size: 4694 724 558 320 BbvCI CC'TCA_GC Cuts at: 565 2993 565 Size: 2428 3868 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 3143 3177 90 Size: 34 3019 34 3209 Fragments arranged by size: 3209 3019 34 34 BciVI GTATCCnnnnn_n' Cuts at: 390 4194 4955 6296 390 Size: 3804 761 1341 390 Fragments arranged by size: 3804 1341 761 390 BclI T'GATC_A Cuts at: 3816 3816 Size: 6296 BglI GCCn_nnn'nGGC Cuts at: 2882 3182 2882 Size: 300 5996 BglII A'GATC_T Cuts at: 2699 3811 5848 2699 Size: 1112 2037 3147 Fragments arranged by size: 3147 2037 1112 BlpI GC'TnA_GC Cuts at: 2376 3172 3408 2376 Size: 796 236 5264 Fragments arranged by size: 5264 796 236 Bme1580I G_kGCm'C Cuts at: 1455 2239 3921 4014 5070 1455 Size: 784 1682 93 1056 2681 Fragments arranged by size: 2681 1682 1056 784 93 BmgBI CAC'GTC Cuts at: 1728 1728 Size: 6296 BmrI ACTGGGnnnn_n' Cuts at: 816 2493 2662 2982 3508 3589 3924 6068 816 Size: 1677 169 320 526 81 335 2144 1044 Fragments arranged by size: 2144 1677 1044 526 335 320 169 81 BmtI G_CTAG'C Cuts at: 683 683 Size: 6296 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 341 2409 2754 4663 341 Size: 2068 345 1909 1974 Fragments arranged by size: 2068 1974 1909 345 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 336 928 1189 1333 1381 1447 1852 2355 2560 Size: 592 261 144 48 66 405 503 205 Cuts at: 2560 2644 2836 2902 3007 336 Size: 84 192 66 105 3625 Fragments arranged by size: 3625 592 503 405 261 205 192 144 105 84 66 66 48 Bpu10I CC'TnA_GC Cuts at: 565 926 1109 1996 2779 2993 565 Size: 361 183 887 783 214 3868 Fragments arranged by size: 3868 887 783 361 214 183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 988 1671 2970 3472 4351 4843 5141 988 Size: 683 1299 502 879 492 298 2143 Fragments arranged by size: 2143 1299 879 683 502 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 691 1810 1860 2058 6000 691 Size: 1119 50 198 3942 987 Fragments arranged by size: 3942 1119 987 198 50 BsaAI yAC'GTr Cuts at: 1278 2843 3649 1278 Size: 1565 806 3925 Fragments arranged by size: 3925 1565 806 BsaBI GATnn'nnATC Cuts at: 6 3810 5847 6 Size: 3804 2037 455 Fragments arranged by size: 3804 2037 455 BsaHI Gr'CG_yC Cuts at: 1387 1577 2108 2204 3727 3975 4677 6173 1387 Size: 190 531 96 1523 248 702 1496 1510 Fragments arranged by size: 1523 1510 1496 702 531 248 190 96 BsaWI w'CCGG_w Cuts at: 572 1550 3485 4006 4684 4958 5105 5516 572 Size: 978 1935 521 678 274 147 411 1352 Fragments arranged by size: 1935 1352 978 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1963 1993 2017 2038 2047 2068 1963 Size: 30 24 21 9 21 6191 Fragments arranged by size: 6191 30 24 21 21 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 322 733 1066 1552 1828 1987 1990 2046 322 Size: 411 333 486 276 159 3 56 4572 Fragments arranged by size: 4572 486 411 333 276 159 56 3 BseYI C'CCAG_C Cuts at: 219 278 931 1046 1328 2793 2880 3721 4479 Size: 59 653 115 282 1465 87 841 758 Cuts at: 4479 5056 5552 219 Size: 577 496 963 Fragments arranged by size: 1465 963 841 758 653 577 496 282 115 87 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 2080 2194 2523 2080 Size: 114 329 5853 Fragments arranged by size: 5853 329 114 BsiEI CG_ry'CG Cuts at: 104 3838 3884 5092 104 Size: 3734 46 1208 1308 Fragments arranged by size: 3734 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 605 953 1004 1175 1495 2048 2149 2239 2576 Size: 348 51 171 320 553 101 90 337 Cuts at: 2576 2918 3310 4088 4278 5070 605 Size: 342 392 778 190 792 1831 Fragments arranged by size: 1831 792 778 553 392 348 342 337 320 190 171 101 90 51 BsmI GAATG_Cn' Cuts at: 895 895 Size: 6296 BsmBI CGTCTCn'nnnn_ Cuts at: 1584 1584 Size: 6296 Bsp1286I G_dGCh'C Cuts at: 605 932 953 1004 1175 1455 1495 1514 1936 Size: 327 21 51 171 280 40 19 422 Cuts at: 1936 2048 2149 2239 2576 2887 2918 3310 3921 Size: 112 101 90 337 311 31 392 611 Cuts at: 3921 4014 4088 4278 4340 5070 605 Size: 93 74 190 62 730 1831 Fragments arranged by size: 1831 730 611 422 392 337 327 311 280 190 171 112 101 93 90 74 62 51 40 31 21 19 BspEI T'CCGG_A Cuts at: 1550 5516 1550 Size: 3966 2330 BspHI T'CATG_A Cuts at: 5472 5472 Size: 6296 BspMI ACCTGCnnnn'nnnn_ Cuts at: 609 1351 1430 3346 3560 3862 4243 609 Size: 742 79 1916 214 302 381 2662 Fragments arranged by size: 2662 1916 742 381 302 214 79 BsrBI CCG'CTC Cuts at: 50 342 4588 4642 6126 6291 50 Size: 292 4246 54 1484 165 55 Fragments arranged by size: 4246 1484 292 165 55 54 BsrDI GCAATG_nn' Cuts at: 543 2027 4208 543 Size: 1484 2181 2631 Fragments arranged by size: 2631 2181 1484 BsrFI r'CCGG_y Cuts at: 259 572 2876 4294 4475 4684 259 Size: 313 2304 1418 181 209 1871 Fragments arranged by size: 2304 1871 1418 313 209 181 BsrGI T'GTAC_A Cuts at: 1409 1409 Size: 6296 BssHII G'CGCG_C Cuts at: 541 935 541 Size: 394 5902 BssSI C'ACGA_G Cuts at: 4567 4925 4567 Size: 358 5938 BstAPI GCAn_nnn'nTGC Cuts at: 3673 3673 Size: 6296 BstBI TT'CG_AA Cuts at: 4657 4657 Size: 6296 BstXI CCAn_nnnn'nTGG Cuts at: 156 1146 1185 156 Size: 990 39 5267 Fragments arranged by size: 5267 990 39 BstYI r'GATC_y Cuts at: 1 233 477 1190 1889 1964 2433 2699 3321 Size: 232 244 713 699 75 469 266 622 Cuts at: 3321 3788 3811 4146 4392 5404 5490 5502 5848 Size: 467 23 335 246 1012 86 12 346 Cuts at: 5848 1 Size: 449 Fragments arranged by size: 1012 713 699 622 469 467 449 346 335 266 246 244 232 86 75 23 12 Bsu36I CC'TnA_GG Cuts at: 1025 2347 2638 2830 1025 Size: 1322 291 192 4491 Fragments arranged by size: 4491 1322 291 192 BtgI C'CryG_G Cuts at: 665 743 884 1064 1139 665 Size: 78 141 180 75 5822 Fragments arranged by size: 5822 180 141 78 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 4246 4427 4590 4246 Size: 181 163 5952 Fragments arranged by size: 5952 181 163 BtsI GCAGTG_nn' Cuts at: 1400 1412 1891 2711 3539 1400 Size: 12 479 820 828 4157 Fragments arranged by size: 4157 828 820 479 12 DraI TTT'AAA Cuts at: 3295 3295 Size: 6296 DraIII CAC_nnn'GTG Cuts at: 409 795 1307 409 Size: 386 512 5398 Fragments arranged by size: 5398 512 386 DrdI GACnn_nn'nnGTC Cuts at: 3148 4002 4860 3148 Size: 854 858 4584 Fragments arranged by size: 4584 858 854 EaeI y'GGCC_r Cuts at: 3881 4055 4446 4473 5818 3881 Size: 174 391 27 1345 4359 Fragments arranged by size: 4359 1345 391 174 27 EagI C'GGCC_G Cuts at: 3881 3881 Size: 6296 EarI CTCTTCn'nnn_ Cuts at: 829 853 1303 1831 2350 4319 4529 829 Size: 24 450 528 519 1969 210 2596 Fragments arranged by size: 2596 1969 528 519 450 210 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 826 4504 4814 4960 6106 826 Size: 3678 310 146 1146 1016 Fragments arranged by size: 3678 1146 1016 310 146 EcoICRI GAG'CTC Cuts at: 951 1002 3308 951 Size: 51 2306 3939 Fragments arranged by size: 3939 2306 51 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 202 336 897 928 1189 1333 1381 1447 1852 Size: 134 561 31 261 144 48 66 405 Cuts at: 1852 1932 2089 2323 2355 2374 2560 2644 2716 Size: 80 157 234 32 19 186 84 72 Cuts at: 2716 2836 2902 3007 4121 4553 5300 5568 202 Size: 120 66 105 1114 432 747 268 930 Fragments arranged by size: 1114 930 747 561 432 405 268 261 234 186 157 144 134 120 105 84 80 72 66 66 48 32 31 19 EcoNI CCTnn'n_nnAGG Cuts at: 1534 1693 1810 1927 1534 Size: 159 117 117 5903 Fragments arranged by size: 5903 159 117 117 EcoO109I rG'GnC_Cy Cuts at: 357 384 1030 1451 1452 1545 1992 2388 2484 Size: 27 646 421 1 93 447 396 96 Cuts at: 2484 2799 2826 2976 3435 357 Size: 315 27 150 459 3218 Fragments arranged by size: 3218 646 459 447 421 396 315 150 96 93 27 27 1 EcoRI G'AATT_C Cuts at: 1663 3300 1663 Size: 1637 4659 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 475 1242 4315 475 Size: 767 3073 2456 Fragments arranged by size: 3073 2456 767 FspI TGC'GCA Cuts at: 4077 4077 Size: 6296 HaeII r_GCGC'y Cuts at: 726 972 2076 2087 2111 3256 3730 3978 5000 Size: 246 1104 11 24 1145 474 248 1022 Cuts at: 5000 5979 726 Size: 979 1043 Fragments arranged by size: 1145 1104 1043 1022 979 474 248 246 24 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2517 2549 4138 4168 4170 4200 2517 Size: 32 1589 30 2 30 4613 Fragments arranged by size: 4613 1589 32 30 30 2 HincII GTy'rAC Cuts at: 437 561 1742 3335 437 Size: 124 1181 1593 3398 Fragments arranged by size: 3398 1593 1181 124 KasI G'GCGC_C Cuts at: 2107 3726 3974 2107 Size: 1619 248 4429 Fragments arranged by size: 4429 1619 248 KpnI G_GTAC'C Cuts at: 3316 3316 Size: 6296 MluI A'CGCG_T Cuts at: 1751 5729 1751 Size: 3978 2318 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2433 2582 3177 4967 5151 2433 Size: 149 595 1790 184 3578 Fragments arranged by size: 3578 1790 595 184 149 MscI TGG'CCA Cuts at: 4057 4057 Size: 6296 MslI CAynn'nnrTG Cuts at: 1405 1870 2102 4285 4412 1405 Size: 465 232 2183 127 3289 Fragments arranged by size: 3289 2183 465 232 127 MspA1I CmG'CkG Cuts at: 282 917 1359 1952 2227 2538 3407 3721 4081 Size: 635 442 593 275 311 869 314 360 Cuts at: 4081 5094 5339 282 Size: 1013 245 1239 Fragments arranged by size: 1239 1013 869 635 593 442 360 314 311 275 245 NaeI GCC'GGC Cuts at: 2878 4477 2878 Size: 1599 4697 NarI GG'CG_CC Cuts at: 2108 3727 3975 2108 Size: 1619 248 4429 Fragments arranged by size: 4429 1619 248 NgoMIV G'CCGG_C Cuts at: 2876 4475 2876 Size: 1599 4697 NheI G'CTAG_C Cuts at: 679 679 Size: 6296 NspI r_CATG'y Cuts at: 443 900 1186 1416 3349 3849 4756 443 Size: 457 286 230 1933 500 907 1983 Fragments arranged by size: 1983 1933 907 500 457 286 230 PasI CC'CwG_GG Cuts at: 239 2280 3087 239 Size: 2041 807 3448 Fragments arranged by size: 3448 2041 807 PciI A'CATG_T Cuts at: 4752 4752 Size: 6296 PflMI CCAn_nnn'nTGG Cuts at: 409 409 Size: 6296 PfoI T'CCnGG_A Cuts at: 944 1390 1658 1688 1967 2936 944 Size: 446 268 30 279 969 4304 Fragments arranged by size: 4304 969 446 279 268 30 PmeI GTTT'AAAC Cuts at: 3295 3295 Size: 6296 PmlI CAC'GTG Cuts at: 2843 2843 Size: 6296 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5435 5467 5435 Size: 32 6264 PpuMI rG'GwC_Cy Cuts at: 357 384 1030 1545 2388 2799 357 Size: 27 646 515 843 411 3854 Fragments arranged by size: 3854 843 646 515 411 27 PshAI GACnn'nnGTC Cuts at: 1386 1386 Size: 6296 PspOMI G'GGCC_C Cuts at: 1451 1451 Size: 6296 PspXI vC'TCGA_Gb Cuts at: 166 166 Size: 6296 PstI C_TGCA'G Cuts at: 865 1407 1928 2080 2400 2513 2677 3019 3343 Size: 542 521 152 320 113 164 342 324 Cuts at: 3343 4028 865 Size: 685 3133 Fragments arranged by size: 3133 685 542 521 342 324 320 164 152 113 PvuI CG_AT'CG Cuts at: 104 104 Size: 6296 PvuII CAG'CTG Cuts at: 282 917 1359 1952 2538 3721 4081 282 Size: 635 442 593 586 1183 360 2497 Fragments arranged by size: 2497 1183 635 593 586 442 360 RsrII CG'GwC_CG Cuts at: 4491 4491 Size: 6296 SacI G_AGCT'C Cuts at: 953 1004 3310 953 Size: 51 2306 3939 Fragments arranged by size: 3939 2306 51 SalI G'TCGA_C Cuts at: 3333 3333 Size: 6296 SanDI GG'GwC_CC Cuts at: 1030 1030 Size: 6296 SapI GCTCTTCn'nnn_ Cuts at: 829 4319 4529 829 Size: 3490 210 2596 Fragments arranged by size: 3490 2596 210 SbfI CC_TGCA'GG Cuts at: 3343 3343 Size: 6296 ScaI AGT'ACT Cuts at: 5813 5813 Size: 6296 SexAI A'CCwGG_T Cuts at: 386 405 386 Size: 19 6277 SfcI C'TryA_G Cuts at: 33 492 861 1403 1635 1924 2076 2115 2208 Size: 459 369 542 232 289 152 39 93 Cuts at: 2208 2396 2509 2673 3015 3339 4024 5017 5208 Size: 188 113 164 342 324 685 993 191 Cuts at: 5208 33 Size: 1121 Fragments arranged by size: 1121 993 685 542 459 369 342 324 289 232 191 188 164 152 113 93 39 SfoI GGC'GCC Cuts at: 2109 3728 3976 2109 Size: 1619 248 4429 Fragments arranged by size: 4429 1619 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6296 SmaI CCC'GGG Cuts at: 3318 3318 Size: 6296 SmlI C'TyrA_G Cuts at: 166 1003 1650 2949 3451 4366 4858 5120 166 Size: 837 647 1299 502 915 492 262 1342 Fragments arranged by size: 1342 1299 915 837 647 502 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 6296 SphI G_CATG'C Cuts at: 900 3349 3849 900 Size: 2449 500 3347 Fragments arranged by size: 3347 2449 500 StuI AGG'CCT Cuts at: 313 313 Size: 6296 StyI C'CwwG_G Cuts at: 546 828 1496 1937 3059 3135 3430 3784 546 Size: 282 668 441 1122 76 295 354 3058 Fragments arranged by size: 3058 1122 668 441 354 295 282 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 307 1320 1488 1759 2681 3124 3879 4012 4471 Size: 1013 168 271 922 443 755 133 459 Cuts at: 4471 4680 307 Size: 209 1923 Fragments arranged by size: 1923 1013 922 755 459 443 271 209 168 133 TatI w'GTAC_w Cuts at: 1409 1679 5811 1409 Size: 270 4132 1894 Fragments arranged by size: 4132 1894 270 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2038 2070 5813 5845 2038 Size: 32 3743 32 2489 Fragments arranged by size: 3743 2489 32 32 Tth111I GACn'n_nGTC Cuts at: 502 4093 502 Size: 3591 2705 XbaI T'CTAG_A Cuts at: 3327 3327 Size: 6296 XcmI CCAnnnn_n'nnnnTGG Cuts at: 311 1827 2589 3791 311 Size: 1516 762 1202 2816 Fragments arranged by size: 2816 1516 1202 762 XhoI C'TCGA_G Cuts at: 166 166 Size: 6296 XmaI C'CCGG_G Cuts at: 3316 3316 Size: 6296 XmnI GAAnn'nnTTC Cuts at: 187 823 1603 187 Size: 636 780 4880 Fragments arranged by size: 4880 780 636 ZraI GAC'GTC Cuts at: 1388 1578 1388 Size: 190 6106 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AflII AhdI AloI AscI BaeI BfrBI BsiWI BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NotI NruI NsiI PacI PsiI PsrI SacII SfiI SgrAI SnaBI SrfI SspI SwaI