Restriction map for
pF1KE6677
(Circular) (Six-base) MAPSORT of: pF1KE6677.seq Check: 1781 from: 1 to: 4004
pF1KE6677 4004 bp
With 182 enzymes: SgfI *
October 17, 2018 19:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 477 477
Size: 4004
AccI GT'mk_AC
Cuts at: 1042 1042
Size: 4004
Acc65I G'GTAC_C
Cuts at: 275 1020 275
Size: 745 3259
AclI AA'CG_TT
Cuts at: 3844 3844
Size: 4004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 352 1829 2261 3008 3276 274
Size: 78 1477 432 747 268 1002
Fragments arranged by size:
1477 1002 747 432 268 78
AflIII A'CryG_T
Cuts at: 197 1356 2460 3437 197
Size: 1159 1104 977 764
Fragments arranged by size:
1159 1104 977 764
AgeI A'CCGG_T
Cuts at: 2392 2392
Size: 4004
AlwNI CAG_nnn'CTG
Cuts at: 1309 2876 1309
Size: 1567 2437
ApaLI G'TGCA_C
Cuts at: 2774 2774
Size: 4004
ApoI r'AATT_y
Cuts at: 811 951 1008 3357 3613 811
Size: 140 57 2349 256 1202
Fragments arranged by size:
2349 1202 256 140 57
AseI AT'TA_AT
Cuts at: 20 20
Size: 4004
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
AvaI C'yCGr_G
Cuts at: 146 607 1024 146
Size: 461 417 3126
Fragments arranged by size:
3126 461 417
BamHI G'GATC_C
Cuts at: 1029 1029
Size: 4004
BanI G'GyrC_C
Cuts at: 275 1020 1434 1682 1717 275
Size: 745 414 248 35 2562
Fragments arranged by size:
2562 745 414 248 35
BanII G_rGCy'C
Cuts at: 442 785 1018 2048 442
Size: 343 233 1030 2398
Fragments arranged by size:
2398 1030 343 233
BbeI G_GCGC'C
Cuts at: 1438 1686 1438
Size: 248 3756
BciVI GTATCCnnnnn_n'
Cuts at: 1902 2663 4004 1902
Size: 761 1341 1902
Fragments arranged by size:
1902 1341 761
BclI T'GATC_A
Cuts at: 1524 1524
Size: 4004
BglII A'GATC_T
Cuts at: 1519 3556 1519
Size: 2037 1967
BlpI GC'TnA_GC
Cuts at: 1116 1116
Size: 4004
Bme1580I G_kGCm'C
Cuts at: 1629 1722 2778 1629
Size: 93 1056 2855
Fragments arranged by size:
2855 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1216 1297 1632 3776 1216
Size: 81 335 2144 1444
Fragments arranged by size:
2144 1444 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 604
Size: 4004
Bpu10I CC'TnA_GC
Cuts at: 385 385
Size: 4004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1180 2059 2551 2849 337
Size: 843 879 492 298 1492
Fragments arranged by size:
1492 879 843 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 667 3708 667
Size: 3041 963
BsaAI yAC'GTr
Cuts at: 321 1357 321
Size: 1036 2968
BsaBI GATnn'nnATC
Cuts at: 6 1518 3555 6
Size: 1512 2037 455
Fragments arranged by size:
2037 1512 455
BsaHI Gr'CG_yC
Cuts at: 515 1435 1683 2385 3881 515
Size: 920 248 702 1496 638
Fragments arranged by size:
1496 920 702 638 248
BsaWI w'CCGG_w
Cuts at: 1193 1714 2392 2666 2813 3224 1193
Size: 521 678 274 147 411 1973
Fragments arranged by size:
1973 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 241 316 373 387 625 160
Size: 81 75 57 14 238 3539
Fragments arranged by size:
3539 238 81 75 57 14
BseYI C'CCAG_C
Cuts at: 1429 2187 2764 3260 1429
Size: 758 577 496 2173
Fragments arranged by size:
2173 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 463 192
Size: 271 3733
BsiEI CG_ry'CG
Cuts at: 104 1546 1592 2800 104
Size: 1442 46 1208 1308
Fragments arranged by size:
1442 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 170 785 1018 1796 1986 2778 170
Size: 615 233 778 190 792 1396
Fragments arranged by size:
1396 792 778 615 233 190
BsmI GAATG_Cn'
Cuts at: 513 513
Size: 4004
Bsp1286I G_dGCh'C
Cuts at: 170 442 785 1018 1629 1722 1796 1986 2048
Size: 272 343 233 611 93 74 190 62
Cuts at: 2048 2778 170
Size: 730 1396
Fragments arranged by size:
1396 730 611 343 272 233 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3224 3224
Size: 4004
BspHI T'CATG_A
Cuts at: 851 3180 851
Size: 2329 1675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 477 1054 1268 1570 1951 477
Size: 577 214 302 381 2530
Fragments arranged by size:
2530 577 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2296 2350 3834 3999 50
Size: 2246 54 1484 165 55
Fragments arranged by size:
2246 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1916 1916
Size: 4004
BsrFI r'CCGG_y
Cuts at: 2002 2183 2392 2002
Size: 181 209 3614
Fragments arranged by size:
3614 209 181
BsrGI T'GTAC_A
Cuts at: 194 194
Size: 4004
BssSI C'ACGA_G
Cuts at: 2275 2633 2275
Size: 358 3646
BstAPI GCAn_nnn'nTGC
Cuts at: 945 1381 945
Size: 436 3568
BstBI TT'CG_AA
Cuts at: 2365 2365
Size: 4004
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4004
BstYI r'GATC_y
Cuts at: 1 743 1029 1496 1519 1854 2100 3112 3198
Size: 742 286 467 23 335 246 1012 86
Cuts at: 3198 3210 3556 1
Size: 12 346 449
Fragments arranged by size:
1012 742 467 449 346 335 286 246
86 23 12
BtgI C'CryG_G
Cuts at: 260 260
Size: 4004
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 425 1954 2135 2298 425
Size: 1529 181 163 2131
Fragments arranged by size:
2131 1529 181 163
BtsI GCAGTG_nn'
Cuts at: 238 548 1247 238
Size: 310 699 2995
Fragments arranged by size:
2995 699 310
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 905 940 905
Size: 35 3969
DraI TTT'AAA
Cuts at: 1003 1003
Size: 4004
DrdI GACnn_nn'nnGTC
Cuts at: 1710 2568 1710
Size: 858 3146
EaeI y'GGCC_r
Cuts at: 524 881 1589 1763 2154 2181 3526 524
Size: 357 708 174 391 27 1345 1002
Fragments arranged by size:
1345 1002 708 391 357 174 27
EagI C'GGCC_G
Cuts at: 1589 1589
Size: 4004
EarI CTCTTCn'nnn_
Cuts at: 2027 2237 2027
Size: 210 3794
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2212 2522 2668 3814 2212
Size: 310 146 1146 2402
Fragments arranged by size:
2402 1146 310 146
EcoICRI GAG'CTC
Cuts at: 783 1016 783
Size: 233 3771
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 352 604 1829 2261 3008 3276 274
Size: 78 252 1225 432 747 268 1002
Fragments arranged by size:
1225 1002 747 432 268 252 78
EcoO109I rG'GnC_Cy
Cuts at: 1143 1143
Size: 4004
EcoRI G'AATT_C
Cuts at: 811 1008 811
Size: 197 3807
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2023 2023
Size: 4004
FspI TGC'GCA
Cuts at: 1785 1785
Size: 4004
HaeII r_GCGC'y
Cuts at: 1438 1686 2708 3687 1438
Size: 248 1022 979 1755
Fragments arranged by size:
1755 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1846 1876 1878 1908 1846
Size: 30 2 30 3942
Fragments arranged by size:
3942 30 30 2
HincII GTy'rAC
Cuts at: 1043 1043
Size: 4004
KasI G'GCGC_C
Cuts at: 1434 1682 1434
Size: 248 3756
KpnI G_GTAC'C
Cuts at: 279 1024 279
Size: 745 3259
MluI A'CGCG_T
Cuts at: 3437 3437
Size: 4004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2675 2859 2675
Size: 184 3820
MscI TGG'CCA
Cuts at: 526 883 1765 526
Size: 357 882 2765
Fragments arranged by size:
2765 882 357
MslI CAynn'nnrTG
Cuts at: 112 806 856 1993 2120 112
Size: 694 50 1137 127 1996
Fragments arranged by size:
1996 1137 694 127 50
MspA1I CmG'CkG
Cuts at: 234 582 1115 1429 1789 2802 3047 234
Size: 348 533 314 360 1013 245 1191
Fragments arranged by size:
1191 1013 533 360 348 314 245
NaeI GCC'GGC
Cuts at: 2185 2185
Size: 4004
NarI GG'CG_CC
Cuts at: 1435 1683 1435
Size: 248 3756
NgoMIV G'CCGG_C
Cuts at: 2183 2183
Size: 4004
NspI r_CATG'y
Cuts at: 201 1057 1557 2464 201
Size: 856 500 907 1741
Fragments arranged by size:
1741 907 856 500
PasI CC'CwG_GG
Cuts at: 245 245
Size: 4004
PciI A'CATG_T
Cuts at: 197 2460 197
Size: 2263 1741
PmeI GTTT'AAAC
Cuts at: 1003 1003
Size: 4004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3143 3175 3143
Size: 32 3972
PstI C_TGCA'G
Cuts at: 368 693 1051 1736 368
Size: 325 358 685 2636
Fragments arranged by size:
2636 685 358 325
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4004
PvuII CAG'CTG
Cuts at: 234 582 1429 1789 234
Size: 348 847 360 2449
Fragments arranged by size:
2449 847 360 348
RsrII CG'GwC_CG
Cuts at: 2199 2199
Size: 4004
SacI G_AGCT'C
Cuts at: 785 1018 785
Size: 233 3771
SalI G'TCGA_C
Cuts at: 1041 1041
Size: 4004
SapI GCTCTTCn'nnn_
Cuts at: 2027 2237 2027
Size: 210 3794
SbfI CC_TGCA'GG
Cuts at: 1051 1051
Size: 4004
ScaI AGT'ACT
Cuts at: 3521 3521
Size: 4004
SfcI C'TryA_G
Cuts at: 33 364 565 689 1047 1732 2725 2916 33
Size: 331 201 124 358 685 993 191 1121
Fragments arranged by size:
1121 993 685 358 331 201 191 124
SfoI GGC'GCC
Cuts at: 1436 1684 1436
Size: 248 3756
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4004
SmaI CCC'GGG
Cuts at: 148 1026 148
Size: 878 3126
SmlI C'TyrA_G
Cuts at: 352 1159 2074 2566 2828 352
Size: 807 915 492 262 1528
Fragments arranged by size:
1528 915 807 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4004
SphI G_CATG'C
Cuts at: 1057 1557 1057
Size: 500 3504
StuI AGG'CCT
Cuts at: 960 960
Size: 4004
StyI C'CwwG_G
Cuts at: 668 1138 1492 668
Size: 470 354 3180
Fragments arranged by size:
3180 470 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1587 1720 2179 2388 1587
Size: 133 459 209 3203
Fragments arranged by size:
3203 459 209 133
TatI w'GTAC_w
Cuts at: 194 3519 194
Size: 3325 679
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 3972
Tth111I GACn'n_nGTC
Cuts at: 1801 1801
Size: 4004
XbaI T'CTAG_A
Cuts at: 1035 1035
Size: 4004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 586 855 1499 586
Size: 269 644 3091
Fragments arranged by size:
3091 644 269
XmaI C'CCGG_G
Cuts at: 146 1024 146
Size: 878 3126
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsiWI BsmBI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI