Restriction map for
pF1KE6622
(Circular) (Six-base) MAPSORT of: pF1KE6622.seq Check: 3830 from: 1 to: 4031
pF1KE6622 4031 bp
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AccI GT'mk_AC
Cuts at: 1069 1069
Size: 4031
Acc65I G'GTAC_C
Cuts at: 1047 1047
Size: 4031
AclI AA'CG_TT
Cuts at: 242 3871 242
Size: 3629 402
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 761 1856 2288 3035 3303 134
Size: 627 1095 432 747 268 862
Fragments arranged by size:
1095 862 747 627 432 268
AfeI AGC'GCT
Cuts at: 334 334
Size: 4031
AflIII A'CryG_T
Cuts at: 1023 1383 2487 3464 1023
Size: 360 1104 977 1590
Fragments arranged by size:
1590 1104 977 360
AgeI A'CCGG_T
Cuts at: 2419 2419
Size: 4031
AlwNI CAG_nnn'CTG
Cuts at: 1336 2903 1336
Size: 1567 2464
ApaLI G'TGCA_C
Cuts at: 2801 2801
Size: 4031
ApoI r'AATT_y
Cuts at: 377 843 1035 3384 3640 377
Size: 466 192 2349 256 768
Fragments arranged by size:
2349 768 466 256 192
AseI AT'TA_AT
Cuts at: 20 20
Size: 4031
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
AvaI C'yCGr_G
Cuts at: 811 1051 811
Size: 240 3791
BamHI G'GATC_C
Cuts at: 1056 1056
Size: 4031
BanI G'GyrC_C
Cuts at: 1047 1461 1709 1744 1047
Size: 414 248 35 3334
Fragments arranged by size:
3334 414 248 35
BanII G_rGCy'C
Cuts at: 1045 2075 1045
Size: 1030 3001
BbeI G_GCGC'C
Cuts at: 1465 1713 1465
Size: 248 3783
BciVI GTATCCnnnnn_n'
Cuts at: 1929 2690 4031 1929
Size: 761 1341 1929
Fragments arranged by size:
1929 1341 761
BclI T'GATC_A
Cuts at: 1551 1551
Size: 4031
BglI GCCn_nnn'nGGC
Cuts at: 900 900
Size: 4031
BglII A'GATC_T
Cuts at: 303 1546 3583 303
Size: 1243 2037 751
Fragments arranged by size:
2037 1243 751
BlpI GC'TnA_GC
Cuts at: 284 1143 284
Size: 859 3172
Bme1580I G_kGCm'C
Cuts at: 1656 1749 2805 1656
Size: 93 1056 2882
Fragments arranged by size:
2882 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1243 1324 1659 3803 1243
Size: 81 335 2144 1471
Fragments arranged by size:
2144 1471 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 713 2398 713
Size: 1685 2346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 199 259 310 164
Size: 35 60 51 3885
Fragments arranged by size:
3885 60 51 35
Bpu10I CC'TnA_GC
Cuts at: 371 371
Size: 4031
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 683 692 1207 2086 2578 2876 683
Size: 9 515 879 492 298 1838
Fragments arranged by size:
1838 879 515 492 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 3735 3735
Size: 4031
BsaAI yAC'GTr
Cuts at: 1384 1384
Size: 4031
BsaBI GATnn'nnATC
Cuts at: 6 1545 3582 6
Size: 1539 2037 455
Fragments arranged by size:
2037 1539 455
BsaHI Gr'CG_yC
Cuts at: 1462 1710 2412 3908 1462
Size: 248 702 1496 1585
Fragments arranged by size:
1585 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1220 1741 2419 2693 2840 3251 1220
Size: 521 678 274 147 411 2000
Fragments arranged by size:
2000 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 320 1456 2214 2791 3287 320
Size: 1136 758 577 496 1064
Fragments arranged by size:
1136 1064 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1573 1619 2827 104
Size: 1469 46 1208 1308
Fragments arranged by size:
1469 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1045 1823 2013 2805 1045
Size: 778 190 792 2271
Fragments arranged by size:
2271 792 778 190
BsmI GAATG_Cn'
Cuts at: 416 416
Size: 4031
BsmBI CGTCTCn'nnnn_
Cuts at: 402 402
Size: 4031
Bsp1286I G_dGCh'C
Cuts at: 1045 1656 1749 1823 2013 2075 2805 1045
Size: 611 93 74 190 62 730 2271
Fragments arranged by size:
2271 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3251 3251
Size: 4031
BspHI T'CATG_A
Cuts at: 917 3207 917
Size: 2290 1741
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1081 1295 1597 1978 1081
Size: 214 302 381 3134
Fragments arranged by size:
3134 381 302 214
BsrBI CCG'CTC
Cuts at: 50 697 774 2323 2377 3861 4026 50
Size: 647 77 1549 54 1484 165 55
Fragments arranged by size:
1549 1484 647 165 77 55 54
BsrDI GCAATG_nn'
Cuts at: 1943 1943
Size: 4031
BsrFI r'CCGG_y
Cuts at: 2029 2210 2419 2029
Size: 181 209 3641
Fragments arranged by size:
3641 209 181
BssSI C'ACGA_G
Cuts at: 2302 2660 2302
Size: 358 3673
BstAPI GCAn_nnn'nTGC
Cuts at: 520 1408 520
Size: 888 3143
BstBI TT'CG_AA
Cuts at: 2392 2392
Size: 4031
BstYI r'GATC_y
Cuts at: 1 303 1056 1523 1546 1881 2127 3139 3225
Size: 302 753 467 23 335 246 1012 86
Cuts at: 3225 3237 3583 1
Size: 12 346 449
Fragments arranged by size:
1012 753 467 449 346 335 302 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4031
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1981 2162 2325 1981
Size: 181 163 3687
Fragments arranged by size:
3687 181 163
BtsI GCAGTG_nn'
Cuts at: 1274 1274
Size: 4031
DraI TTT'AAA
Cuts at: 1030 1030
Size: 4031
DrdI GACnn_nn'nnGTC
Cuts at: 1737 2595 1737
Size: 858 3173
EaeI y'GGCC_r
Cuts at: 901 1616 1790 2181 2208 3553 901
Size: 715 174 391 27 1345 1379
Fragments arranged by size:
1379 1345 715 391 174 27
EagI C'GGCC_G
Cuts at: 1616 1616
Size: 4031
EarI CTCTTCn'nnn_
Cuts at: 470 2054 2264 470
Size: 1584 210 2237
Fragments arranged by size:
2237 1584 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2239 2549 2695 3841 2239
Size: 310 146 1146 2429
Fragments arranged by size:
2429 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1043 1043
Size: 4031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 164 199 259 310 761 1856 2288 3035
Size: 30 35 60 51 451 1095 432 747
Cuts at: 3035 3303 134
Size: 268 862
Fragments arranged by size:
1095 862 747 451 432 268 60 51
35 30
EcoO109I rG'GnC_Cy
Cuts at: 1170 1170
Size: 4031
EcoRI G'AATT_C
Cuts at: 1035 1035
Size: 4031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 513 682 2050 513
Size: 169 1368 2494
Fragments arranged by size:
2494 1368 169
FspI TGC'GCA
Cuts at: 1812 1812
Size: 4031
HaeII r_GCGC'y
Cuts at: 336 1465 1713 2735 3714 336
Size: 1129 248 1022 979 653
Fragments arranged by size:
1129 1022 979 653 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 210 242 769 801 1873 1903 1905 1935 210
Size: 32 527 32 1072 30 2 30 2306
Fragments arranged by size:
2306 1072 527 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1070 1070
Size: 4031
HindIII A'AGCT_T
Cuts at: 752 752
Size: 4031
KasI G'GCGC_C
Cuts at: 1461 1709 1461
Size: 248 3783
KpnI G_GTAC'C
Cuts at: 1051 1051
Size: 4031
MluI A'CGCG_T
Cuts at: 3464 3464
Size: 4031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2702 2886 2702
Size: 184 3847
MscI TGG'CCA
Cuts at: 1792 1792
Size: 4031
MslI CAynn'nnrTG
Cuts at: 2020 2147 2020
Size: 127 3904
MspA1I CmG'CkG
Cuts at: 249 1142 1456 1816 2829 3074 249
Size: 893 314 360 1013 245 1206
Fragments arranged by size:
1206 1013 893 360 314 245
NaeI GCC'GGC
Cuts at: 2212 2212
Size: 4031
NarI GG'CG_CC
Cuts at: 1462 1710 1462
Size: 248 3783
NcoI C'CATG_G
Cuts at: 107 107
Size: 4031
NgoMIV G'CCGG_C
Cuts at: 2210 2210
Size: 4031
NspI r_CATG'y
Cuts at: 1027 1084 1584 2491 1027
Size: 57 500 907 2567
Fragments arranged by size:
2567 907 500 57
PciI A'CATG_T
Cuts at: 1023 2487 1023
Size: 1464 2567
PflMI CCAn_nnn'nTGG
Cuts at: 192 192
Size: 4031
PfoI T'CCnGG_A
Cuts at: 176 176
Size: 4031
PmeI GTTT'AAAC
Cuts at: 1030 1030
Size: 4031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3999
PstI C_TGCA'G
Cuts at: 209 1078 1763 209
Size: 869 685 2477
Fragments arranged by size:
2477 869 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4031
PvuII CAG'CTG
Cuts at: 1456 1816 1456
Size: 360 3671
RsrII CG'GwC_CG
Cuts at: 2226 2226
Size: 4031
SacI G_AGCT'C
Cuts at: 1045 1045
Size: 4031
SalI G'TCGA_C
Cuts at: 1068 1068
Size: 4031
SapI GCTCTTCn'nnn_
Cuts at: 2054 2264 2054
Size: 210 3821
SbfI CC_TGCA'GG
Cuts at: 209 1078 209
Size: 869 3162
ScaI AGT'ACT
Cuts at: 3548 3548
Size: 4031
SfcI C'TryA_G
Cuts at: 33 205 1074 1759 2752 2943 33
Size: 172 869 685 993 191 1121
Fragments arranged by size:
1121 993 869 685 191 172
SfiI GGCCn_nnn'nGGCC
Cuts at: 900 900
Size: 4031
SfoI GGC'GCC
Cuts at: 1463 1711 1463
Size: 248 3783
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
SmaI CCC'GGG
Cuts at: 1053 1053
Size: 4031
SmlI C'TyrA_G
Cuts at: 698 707 1186 2101 2593 2855 698
Size: 9 479 915 492 262 1874
Fragments arranged by size:
1874 915 492 479 262 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4031
SphI G_CATG'C
Cuts at: 1084 1584 1084
Size: 500 3531
StyI C'CwwG_G
Cuts at: 107 767 1165 1519 107
Size: 660 398 354 2619
Fragments arranged by size:
2619 660 398 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1614 1747 2206 2415 1614
Size: 133 459 209 3230
Fragments arranged by size:
3230 459 209 133
TatI w'GTAC_w
Cuts at: 3546 3546
Size: 4031
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3548 3580 3548
Size: 32 3999
Tth111I GACn'n_nGTC
Cuts at: 1828 1828
Size: 4031
XbaI T'CTAG_A
Cuts at: 1062 1062
Size: 4031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1526 1526
Size: 4031
XmaI C'CCGG_G
Cuts at: 1051 1051
Size: 4031
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BsaXI BseRI BsgI BsiWI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI