Restriction map for
pF1KE6561
(Circular) (Six-base) MAPSORT of: pF1KE6561.seq Check: 8767 from: 1 to: 3593
pF1KE6561 3593 bp
With 182 enzymes: SgfI *
November 28, 2016 14:00 ..
AccI GT'mk_AC
Cuts at: 631 631
Size: 3593
Acc65I G'GTAC_C
Cuts at: 609 609
Size: 3593
AclI AA'CG_TT
Cuts at: 3433 3433
Size: 3593
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 1418 1850 2597 2865 315
Size: 1103 432 747 268 1043
Fragments arranged by size:
1103 1043 747 432 268
AflIII A'CryG_T
Cuts at: 945 2049 3026 945
Size: 1104 977 1512
Fragments arranged by size:
1512 1104 977
AgeI A'CCGG_T
Cuts at: 1981 1981
Size: 3593
AlwNI CAG_nnn'CTG
Cuts at: 898 2465 898
Size: 1567 2026
ApaLI G'TGCA_C
Cuts at: 2363 2363
Size: 3593
ApoI r'AATT_y
Cuts at: 416 597 2946 3202 416
Size: 181 2349 256 807
Fragments arranged by size:
2349 807 256 181
AseI AT'TA_AT
Cuts at: 20 20
Size: 3593
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
AvaI C'yCGr_G
Cuts at: 613 613
Size: 3593
BamHI G'GATC_C
Cuts at: 618 618
Size: 3593
BanI G'GyrC_C
Cuts at: 467 609 1023 1271 1306 467
Size: 142 414 248 35 2754
Fragments arranged by size:
2754 414 248 142 35
BanII G_rGCy'C
Cuts at: 607 1637 607
Size: 1030 2563
BbeI G_GCGC'C
Cuts at: 1027 1275 1027
Size: 248 3345
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3559
BciVI GTATCCnnnnn_n'
Cuts at: 1491 2252 3593 1491
Size: 761 1341 1491
Fragments arranged by size:
1491 1341 761
BclI T'GATC_A
Cuts at: 1113 1113
Size: 3593
BglI GCCn_nnn'nGGC
Cuts at: 529 529
Size: 3593
BglII A'GATC_T
Cuts at: 1108 3145 1108
Size: 2037 1556
BlpI GC'TnA_GC
Cuts at: 705 705
Size: 3593
Bme1580I G_kGCm'C
Cuts at: 1218 1311 2367 1218
Size: 93 1056 2444
Fragments arranged by size:
2444 1056 93
BmgBI CAC'GTC
Cuts at: 144 144
Size: 3593
BmrI ACTGGGnnnn_n'
Cuts at: 805 886 1221 3365 805
Size: 81 335 2144 1033
Fragments arranged by size:
2144 1033 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1960 1960
Size: 3593
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 1648 2140 2438 769
Size: 879 492 298 1924
Fragments arranged by size:
1924 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3297 3297
Size: 3593
BsaAI yAC'GTr
Cuts at: 946 946
Size: 3593
BsaBI GATnn'nnATC
Cuts at: 6 1107 3144 6
Size: 1101 2037 455
Fragments arranged by size:
2037 1101 455
BsaHI Gr'CG_yC
Cuts at: 1024 1272 1974 3470 1024
Size: 248 702 1496 1147
Fragments arranged by size:
1496 1147 702 248
BsaWI w'CCGG_w
Cuts at: 782 1303 1981 2255 2402 2813 782
Size: 521 678 274 147 411 1562
Fragments arranged by size:
1562 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 103 103
Size: 3593
BseYI C'CCAG_C
Cuts at: 1018 1776 2353 2849 1018
Size: 758 577 496 1762
Fragments arranged by size:
1762 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 179 1135 1181 2389 104
Size: 75 956 46 1208 1308
Fragments arranged by size:
1308 1208 956 75 46
BsiHKAI G_wGCw'C
Cuts at: 607 1385 1575 2367 607
Size: 778 190 792 1833
Fragments arranged by size:
1833 792 778 190
BsmI GAATG_Cn'
Cuts at: 335 335
Size: 3593
BsmBI CGTCTCn'nnnn_
Cuts at: 174 174
Size: 3593
Bsp1286I G_dGCh'C
Cuts at: 607 1218 1311 1385 1575 1637 2367 607
Size: 611 93 74 190 62 730 1833
Fragments arranged by size:
1833 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2813 2813
Size: 3593
BspHI T'CATG_A
Cuts at: 2769 2769
Size: 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 380 643 857 1159 1540 380
Size: 263 214 302 381 2433
Fragments arranged by size:
2433 381 302 263 214
BsrBI CCG'CTC
Cuts at: 50 1885 1939 3423 3588 50
Size: 1835 54 1484 165 55
Fragments arranged by size:
1835 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 207 1505 207
Size: 1298 2295
BsrFI r'CCGG_y
Cuts at: 1591 1772 1981 1591
Size: 181 209 3203
Fragments arranged by size:
3203 209 181
BssSI C'ACGA_G
Cuts at: 1864 2222 1864
Size: 358 3235
BstAPI GCAn_nnn'nTGC
Cuts at: 970 970
Size: 3593
BstBI TT'CG_AA
Cuts at: 1954 1954
Size: 3593
BstXI CCAn_nnnn'nTGG
Cuts at: 287 287
Size: 3593
BstYI r'GATC_y
Cuts at: 1 618 1085 1108 1443 1689 2701 2787 2799
Size: 617 467 23 335 246 1012 86 12
Cuts at: 2799 3145 1
Size: 346 449
Fragments arranged by size:
1012 617 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 246 246
Size: 3593
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1543 1724 1887 1543
Size: 181 163 3249
Fragments arranged by size:
3249 181 163
BtsI GCAGTG_nn'
Cuts at: 836 836
Size: 3593
DraI TTT'AAA
Cuts at: 592 592
Size: 3593
DrdI GACnn_nn'nnGTC
Cuts at: 1299 2157 1299
Size: 858 2735
EaeI y'GGCC_r
Cuts at: 449 1178 1352 1743 1770 3115 449
Size: 729 174 391 27 1345 927
Fragments arranged by size:
1345 927 729 391 174 27
EagI C'GGCC_G
Cuts at: 1178 1178
Size: 3593
EarI CTCTTCn'nnn_
Cuts at: 246 373 1616 1826 246
Size: 127 1243 210 2013
Fragments arranged by size:
2013 1243 210 127
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1801 2111 2257 3403 1801
Size: 310 146 1146 1991
Fragments arranged by size:
1991 1146 310 146
EcoICRI GAG'CTC
Cuts at: 605 605
Size: 3593
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 315 1418 1850 2597 2865 315
Size: 1103 432 747 268 1043
Fragments arranged by size:
1103 1043 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 379 379
Size: 3593
EcoO109I rG'GnC_Cy
Cuts at: 732 732
Size: 3593
EcoRI G'AATT_C
Cuts at: 597 597
Size: 3593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1612 1612
Size: 3593
FspI TGC'GCA
Cuts at: 1374 1374
Size: 3593
HaeII r_GCGC'y
Cuts at: 1027 1275 2297 3276 1027
Size: 248 1022 979 1344
Fragments arranged by size:
1344 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1435 1465 1467 1497 1435
Size: 30 2 30 3531
Fragments arranged by size:
3531 30 30 2
HincII GTy'rAC
Cuts at: 632 632
Size: 3593
KasI G'GCGC_C
Cuts at: 1023 1271 1023
Size: 248 3345
KpnI G_GTAC'C
Cuts at: 613 613
Size: 3593
MluI A'CGCG_T
Cuts at: 3026 3026
Size: 3593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2264 2448 2264
Size: 184 3409
MscI TGG'CCA
Cuts at: 1354 1354
Size: 3593
MslI CAynn'nnrTG
Cuts at: 565 1582 1709 565
Size: 1017 127 2449
Fragments arranged by size:
2449 1017 127
MspA1I CmG'CkG
Cuts at: 554 704 1018 1378 2391 2636 554
Size: 150 314 360 1013 245 1511
Fragments arranged by size:
1511 1013 360 314 245 150
NaeI GCC'GGC
Cuts at: 1774 1774
Size: 3593
NarI GG'CG_CC
Cuts at: 1024 1272 1024
Size: 248 3345
NgoMIV G'CCGG_C
Cuts at: 1772 1772
Size: 3593
NspI r_CATG'y
Cuts at: 646 1146 2053 646
Size: 500 907 2186
Fragments arranged by size:
2186 907 500
PciI A'CATG_T
Cuts at: 2049 2049
Size: 3593
PmeI GTTT'AAAC
Cuts at: 592 592
Size: 3593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2732 2764 2732
Size: 32 3561
PstI C_TGCA'G
Cuts at: 296 640 1325 296
Size: 344 685 2564
Fragments arranged by size:
2564 685 344
PvuI CG_AT'CG
Cuts at: 104 179 104
Size: 75 3518
PvuII CAG'CTG
Cuts at: 1018 1378 1018
Size: 360 3233
RsrII CG'GwC_CG
Cuts at: 1788 1788
Size: 3593
SacI G_AGCT'C
Cuts at: 607 607
Size: 3593
SalI G'TCGA_C
Cuts at: 630 630
Size: 3593
SapI GCTCTTCn'nnn_
Cuts at: 1616 1826 1616
Size: 210 3383
SbfI CC_TGCA'GG
Cuts at: 640 640
Size: 3593
ScaI AGT'ACT
Cuts at: 3110 3110
Size: 3593
SfcI C'TryA_G
Cuts at: 33 231 292 636 1321 2314 2505 33
Size: 198 61 344 685 993 191 1121
Fragments arranged by size:
1121 993 685 344 198 191 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 529 529
Size: 3593
SfoI GGC'GCC
Cuts at: 1025 1273 1025
Size: 248 3345
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
SmaI CCC'GGG
Cuts at: 615 615
Size: 3593
SmlI C'TyrA_G
Cuts at: 748 1663 2155 2417 748
Size: 915 492 262 1924
Fragments arranged by size:
1924 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3593
SphI G_CATG'C
Cuts at: 646 1146 646
Size: 500 3093
StyI C'CwwG_G
Cuts at: 380 727 1081 380
Size: 347 354 2892
Fragments arranged by size:
2892 354 347
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 516 1176 1309 1768 1977 516
Size: 660 133 459 209 2132
Fragments arranged by size:
2132 660 459 209 133
TatI w'GTAC_w
Cuts at: 3108 3108
Size: 3593
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 3561
Tth111I GACn'n_nGTC
Cuts at: 1390 1390
Size: 3593
XbaI T'CTAG_A
Cuts at: 624 624
Size: 3593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 421 1088 421
Size: 667 2926
XmaI C'CCGG_G
Cuts at: 613 613
Size: 3593
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsrGI BssHII BstEII
BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI